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| Registros recuperados: 33 | |
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| Robalino, Javier; Chapman, Robert W.; De La Vega, Enrique; O'Leary, Nuala A.; Gorbach, Danielle M.; Du, Zhi-qiang; Rothschild, Max F.; Browdy, Craig L.; Warr, Gregory; Labreuche, Yannick. |
| This chapter contains sections titled: Introduction EST Collection as an Approach to Gene Discovery in Shrimp Medium- to High-Throughput Studies of Differential Expression and Gene Discovery RNAi-Based Applications in Shrimp Aquaculture: From Reverse Genetics to Control of Diseases Markers, Genetic Maps, and Large Insert Genomic Libraries in Shrimp Analytical Challenges in Genomics and Genetics of Shrimp Concluding Remarks Acknowledgments References |
| Tipo: Text |
Palavras-chave: Penaeid shrimp EST collection; Gene discovery gene expression “snapshot” gametogenesis abiotic stress responses differential expression cloning RNAi-based applications; Shrimp targeted gene silencing RNAi; And gene function genetic markers in breeding. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00070/18149/15775.pdf |
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| Garnier, Matthieu; Labreuche, Yannick; Nicolas, Jean-louis. |
| Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within... |
| Tipo: Text |
Palavras-chave: Polyphasic approach; Summer mortalities; Crassostrea gigas; Pathogen; French isolates; Vibrio aestuarianus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4961.pdf |
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| Lemire, Astrid; Goudenege, David; Versigny, Tiphaine; Petton, Bruno; Calteau, Alexandra; Labreuche, Yannick; Le Roux, Frederique. |
| Disease in oysters has been steadily rising over the past decade, threatening the long-term survival of commercial and natural stocks. Our understanding and management of such diseases are of critical importance as aquaculture is an important aspect of dealing with the approaching worldwide food shortage. Although some bacteria of the Vibrio genus isolated from diseased oysters have been demonstrated to be pathogenic by experimental infection, direct causality has not been established. Little is known about the dynamics of how the bacterial population hosted by oysters changes during disease progression. Combining experimental ecology, a high-throughput infection assay and genome sequencing, we show that the onset of disease in oysters is associated with... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00244/35565/34107.pdf |
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| Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis. |
| Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf |
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| Bruto, Maxime; James, Adele; Petton, Bruno; Labreuche, Yannick; Chenivesse, Sabine; Alunno-bruscia, Marianne; Polz, Martin F.; Le Roux, Frederique. |
| Vibrios are frequently associated with oyster mortality; however whether they are the primary causative agent or secondary opportunistic colonizers is not well understood. Here we combine analysis of natural infection dynamics, population genomics and molecular genetics to ask (i) to what extent oysters are passively colonized by Vibrio population present in the surrounding water, (ii) how populations turn over during pathogenicity events and (iii) what genetic factors are responsible for pathogenicity. We identified several populations of Vibrio preferentially associated with oyster tissues. Among these, Vibrio crassostreae is particularly abundant in diseased animals while nearly absent in the surrounding water, and its pathogenicity is correlated with... |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00360/47137/49044.pdf |
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| Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
| Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
| Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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| Labreuche, Yannick; Pallandre, Laurane; Ansquer, Dominique; Herlin, Jose; Wapotro, Billy; Le Roux, Frederique. |
| Two recurring syndromes threaten the viability of the shrimp industry in New Caledonia, which represents the second largest export business. The "Syndrome 93" is a cold season disease due to Vibrio penaeicida affecting all shrimp farms, while the "Summer Syndrome" is a geographically restricted vibriosis caused by a virulent lineage of Vibrio nigripulchritudo. Microbiological procedures for diagnosis of these diseases are time-consuming and do not have the ability to discriminate the range of virulence potentials of V. nigripulchritudo. In this study, we developed a multiplex PCR method to simultaneously detect these two bacterial species and allow for pathotype discrimination. The detection limits of this assay, that includes an internal amplification... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00063/17398/15169.pdf |
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| Labreuche, Yannick; Veloso, Artur; De La Vega, Enrique; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.. |
| Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both... |
| Tipo: Text |
Palavras-chave: Shrimp; Litopenaeus vannamei; RNAi; DsRNA; Argonaute; Sid-1; Antiviral immunity. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12652/9587.pdf |
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| Beliaeff, Benoit; Laugier, Thierry; Chim, Liet; Coatanea, Denis; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Lemaire, Pierrette; Lemonnier, Hugues; Morvan, Patrice; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Soulard, Benoit; Wabete, Nelly; Walling, Emilie; Ansquer, Dominique; Castex, Mathieu; Lucas, Ronan; Reynaud, Yann; Ifremer, LEAD-NC,. |
| Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces,... |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00199/31006/35663.pdf |
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| Le Roux, Frederique; Labreuche, Yannick; Davis, Brigid M.; Iqbal, Naeem; Mangenot, Sophie; Goarant, Cyrille; Mazel, Didier; Waldor, Matthew K.. |
| Vibrioses are the predominant bacterial infections in marine shrimp farms. Vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp Litopenaeus stylirostris in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic V. nigripulchritudo isolates from New Caledonia all cluster into a monophyletic clade and contain a small plasmid, pB1067. Here, we report that a large plasmid, pA1066 (247 kb), can also serve as a marker for virulent V. nigripulchritudo, and that an ancestral version of this plasmid was likely acquired prior to other virulence-linked markers. Additionally, we demonstrate that... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14196/11469.pdf |
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| Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine. |
| Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional... |
| Tipo: Text |
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf |
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| Huvet, Arnaud; Degremont, Lionel; Labreuche, Yannick; Daniel, Jean-yves; Dubrunfaut, Thomas; Boudry, Pierre; Haure, Joel; Bedier, Edouard; Ropert, Michel; Herpin, Amaury; Cunningham, Charles; Moal, Jeanne; Samain, Jean-francois. |
| Summer mortality associated with juveniles of the oyster Crassostrea gigas is the result of a complex interaction between the host, pathogens and environment. Divergent selection based on summer survival have been applied to produce families with resistant and susceptible progeny. Here, we describe the use of suppression subtractive hybridization to generate 150 clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these 150 clones was determined. Among the 25 unique sequences that matched with the products of known genes, those putatively implicated in energy generation (12%) and immune function (16%), systems suspected to be implicated in summer mortality, were extended and analyzed by real... |
| Tipo: Text |
Palavras-chave: Selection; Gene; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3447.pdf |
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| Oyanedel, Daniel; Labreuche, Yannick; Bruto, Maxime; Amraoui, Hajar; Robino, Etienne; Haffner, Philippe; Rubio, Tristan; Charrière, Guillaume; Le Roux, Frederique; Destoumieux-garzón, Delphine. |
| A major debate in evolutionary biology is whether virulence is maintained as an adaptive trait and/or evolves to non‐virulence. In the environment, virulence traits of non‐obligatory parasites are subjected to diverse selective pressures and trade‐offs. Here we focus on a population of Vibrio splendidus that displays moderate virulence for oysters. A MARTX (Multifunctional‐autoprocessing repeats‐in‐toxin) and a type‐six secretion system (T6SS) were found to be necessary for virulence toward oysters, while a region (wbe) involved in O‐antigen synthesis is necessary for resistance to predation against amoebae. Gene inactivation within the wbe region had major consequences on the O‐antigen structure, conferring lower immunogenicity, competitive advantage and... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00619/73070/72194.pdf |
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| Aguirre-guzman, Gabriel; Labreuche, Yannick; Ansquer, Dominique; Espiau, Benoit; Levy, Peva; Ascencio, Felipe; Saulnier, Denis. |
| The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus stylirostris; Vibrio nigripulchritudo; Vibrio penaeicida. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2807.pdf |
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| Labreuche, Yannick; Le Roux, Frederique; Henry, Joel; Zatylny, Celine; Huvet, Arnaud; Lambert, Christophe; Soudant, Philippe; Mazel, Didier; Nicolas, Jean-louis. |
| Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; Metalloprotease; Crassostrea gigas; Oyster; Hemocytes; Extracellular products. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12649/9588.pdf |
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| Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.. |
| injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection... |
| Tipo: Text |
Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf |
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| Piel, Damien; Bruto, Maxime; James, Adele; Labreuche, Yannick; Lambert, Christophe; Janicot, Adrian; Chenivesse, Sabine; Petton, Bruno; Wegner, K. Mathias; Stoudmann, Candice; Blokesch, Melanie; Le Roux, Frederique. |
| Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00510/62129/66343.pdf |
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| Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly. |
| New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government... |
| Tipo: Text |
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf |
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| Charme, Marion; Ansquer, Dominique; Vourey, Elodie; Walling, Emilie; Beliaeff, Benoit; Labreuche, Yannick. |
| Experimental infections together with epidemiological studies have shown that pathogenic and non‐pathogenic isolates of V. nigripulchritudo co‐existed in shrimp farm environment (2, 3). Moreover, obtained results also demonstrated that the “summer syndrome”was caused by a single, possibly emerging, cluster of virulent strains. Consequently, it was hypothesized that pathogenic strains of V. nigripulchritudo may persist from one year to the next in the shrimp farm environment and re‐develop inside the grow‐out system at the following rearing cycle (3). This study was therefore aimed at determining whether V. nigripulchritudo isolates may survive, or not, in a shrimp pond bottom soil during a 18‐week drying period. To this end, V.nigripulchritudo mapping was... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00197/30874/29242.pdf |
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| Registros recuperados: 33 | |
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