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| Registros recuperados: 97 | |
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| Garry, Pascal; Le Saux, Jean-claude; Hervio Heath, Dominique; Le Guyader, Soizick. |
| La Microbiologie sanitaire, thème de recherche fédérant notre laboratoire, nous a permis d’obtenir des résultats intéressants qui sont présentés dans ce rapport et ont été valorisés par des publications ou la participation des membres de l’équipe à des congrès scientifiques. Cette thématique nous permet surtout des collaborations variées au travers des projets de recherche portés par l’équipe. L’élargissement de nos activités en intégrant la coordination REMI constitue un point important à souligner complétant nos actions de référence, d’expertise et de recherche. L’attrait et l’intérêt scientifique suscité par la microbiologie sanitaire a été démontré par le succès rencontré par le colloque organisé à Brest en lien avec la fin du projet européen... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00380/49138/49574.pdf |
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| Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. |
| The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... |
| Tipo: Text |
Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. |
| Ano: 2000 |
URL: https://archimer.ifremer.fr/doc/00507/61879/65953.pdf |
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| Le Saux, Jean-claude; Serais, Ophelie; Krol, Joanna; Parnaudeau, Sylvain; Salvagnac, P.; Delmas, G.; Cicchelero, V.; Claudet, J.; Pothier, P.; Balay, K.; Fiandrino, Annie; Pommepuy, Monique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish have long been identified. Over the past century, various strategies have been set up in shellfish growing areas throughout the world to guarantee the sanitary quality of shellfish and to protect consumers. However despite sanitary improvements, human enteric viruses - especially Hepatitis A virus and norovirus– have been found to be associated with shellfish outbreaks. A recent example demonstrated the impact of storm events. Following heavy rain and sewage overflow, shellfish beds were contaminated and the shellfish from them were marketed after depuration. However, since viruses persist longer than fecal contamination indicator bacteria, several clusters of gastroenteritis cases were... |
| Tipo: Text |
Palavras-chave: Virus; Shellfish; Contamination events. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17736/15257.pdf |
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| Pommepuy, Monique; Le Saux, Jean-claude; Hervio Heath, Dominique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish like oysters, mussels, cockles and clams, have long been identified. Bacterial diseases such as cholera and typhoid fever were the first to be suspected of being linked to consumption of contaminated shellfish (Butt et al., 2004). During the past century, various strategies have been established in shellfish growing areas throughout the world to assure the sanitary quality of shellfish. More recently, despite sanitary surveys, Vibrio parahaemolyticus, Vibrio vulnificus and enteric viruses - especially Hepatitis A virus (HAV) and norovirus (NoV) – were found to be associated in outbreaks of human illness. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12607/9485.pdf |
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| Loisy, Fabienne; Atmar, R; Le Saux, Jean-claude; Cohen, J; Caprais, Marie-paule; Pommepuy, Monique; Le Guyader, Soizick. |
| Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue)... |
| Tipo: Text |
Palavras-chave: Seawater; Viral diseases; Bioaccumulation; Depuration; Contamination; Model; Pancreas; Regression analysis; Shellfish; Phages; Virus. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1233.pdf |
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| Verhoef, L; Depoortere, E; Boxman, I; Duizer, E; Van Duynhoven, Y; Harris, J; Johnsen, C; Kroneman, A; Le Guyader, Soizick; Lim, W; Maunula, L; Meldal, H; Ratcliff, R; Reuter, G; Schreier, E; Siebenga, J; Vainio, K; Varela, C; Vennema, H; Koopmans, M. |
| In June 2006, reported outbreaks of norovirus on cruise ships suddenly increased; 43 outbreaks occurred on 13 vessels. All outbreaks investigated manifested person-to-person transmission. Detection of a point source was impossible because of limited investigation of initial outbreaks and data sharing. The most probable explanation for these outbreaks is increased norovirus activity in the community, which coincided with the emergence of 2 new GGII.4 variant strains in Europe and the Pacific. As in 2002, a new GGII.4 variant detected in the spring and summer corresponded with high norovirus activity in the subsequent winter. Because outbreaks on cruise ships are likely to occur when new variants circulate,. an active reporting system could function as an... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3942.pdf |
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| Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.. |
| An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to... |
| Tipo: Text |
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf |
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| De Graaf, Miranda; Beck, Relja; Caccio, Simone M.; Duim, Birgitta; Fraaij, Pieter L. A.; Le Guyader, Soizick; Lecuit, Marc; Le Pendu, Jacques; De Wit, Emmie; Schultsz, Constance. |
| Bacterial, viral and parasitic zoonotic pathogens that transmit via the fecal-oral route have a major impact on global health. However, the mechanisms underlying the emergence of such pathogens from the animal reservoir and their persistence in the human population are poorly understood. Here, we present a framework of human-to-human transmission of zoonotic pathogens that considers the factors relevant for fecal-oral human-to-human transmission route at the levels of host, pathogen, and environment. We discuss current data gaps and propose future research directions. |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00358/46959/46873.pdf |
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| Miura, Takayuki; Parnaudeau, Sylvain; Grodzki, Marco; Okabe, Satoshi; Atmar, Robert L.; Le Guyader, Soizick. |
| Norovirus is the most common agent implicated in food-borne outbreaks and is frequently detected in environmental samples. These viruses are highly diverse, and three genogroups (genogroup I [GI], GII, and GIV) infect humans. Being noncultivable viruses, real-time reverse transcription-PCR (RT-PCR) is the only sensitive method available for their detection in food or environmental samples. Selection of consensus sequences for the design of sensitive assays has been challenging due to sequence diversity and has led to the development of specific real-time RT-PCR assays for each genogroup. Thus, sample screening can require several replicates for amplification of each genogroup (without considering positive and negative controls or standard curves). This... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00162/27313/25544.pdf |
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| Butot, Sophie; Le Guyader, Soizick; Krol, Joanna; Putallaz, Thierry; Amoroso, Richard; Sanchez, Gloria. |
| Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 NoV samples, including different subtypes of NoV genogroup I and II. The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized kits evaluated failed to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3,... |
| Tipo: Text |
Palavras-chave: Norovirus; Real-time RT-PCR; Commercial kit. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11760/8473.pdf |
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| Zidane, Mohamed; Le Guyader, Soizick; Pommepuy, Monique. |
| During the winter 2000/2001, several outbreaks associated with the consumption of shellfish were declared in France. Following these events, a close cooperation was organized between administrations and laboratories (DGAL, DPMA, InVS, AFSSA, Ifremer, CHU-Dijon and CNC) to gather competences and to manage the situation. This report synthesizes the information collected during these outbreaks. From the outset, the epidemiologic investigations directed the research towards viral contamination and shellfish as contamination source. Several outbreaks were investigated. Oysters and stools samples from ill patients were analysed by RT-PCR for viruses (enterovirus, rotavirus, norovirus and astrovirus). The genic amplification was done by using the same primers in... |
| Tipo: Text |
Palavras-chave: Épidémie; Gastro-entérites; Huîtres; Contamination virale; RT-PCR; Norovirus; TIAC. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00068/17941/15476.pdf |
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| Bougeard, Morgane; Le Saux, Jean-claude; Perenne, Nicolas; Le Guyader, Soizick; Pommepuy, Monique. |
| During rainstorms, watersheds can introduce large amounts of faecal pollution into the rivers and sea, leading to shellfish contamination. In this study, we assessed Escherichia coli fluxes from a catchment, and their impact on estuarine water quality, using two assembled models. For the catchment, the agro-hydrological model SWAT was implemented integrating land uses, soil, topography, rainfall and other climatic data on Daoulas watershed (France). Initially, the SWAT model was calibrated and validated for river flow rates, and results were found satisfactory. Subsequently, different faecal contamination sources were integrated into the model: point sources (WWTP discharges into rivers) and non-point sources (manure spreading on fields). During rainfall... |
| Tipo: Text |
Palavras-chave: Modeling; SWAT; MARS; E. coli; Shellfish; Catchment; Estuary. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17748/15270.pdf |
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| Sima, Laura C.; Schaeffer, Julien; Le Saux, Jean-claude; Parnaudeau, Sylvain; Elimelech, Menachem; Le Guyader, Soizick. |
| To evaluate membrane bioreactor wastewater treatment virus removal, a study was conducted in southwest France. Samples collected from plant influent, an aeration basin, membrane effluent, solid sludge, and effluent biweekly from October 2009 to June 2010 were analyzed for calicivirus (norovirus and sapovirus) by real-time reverse transcription-PCR (RT-PCR) using extraction controls to perform quantification. Adenovirus and Escherichia coli also were analyzed to compare removal efficiencies. In the influent, sapovirus was always present, while the norovirus concentration varied temporally, with the highest concentration being detected from February to May. All three human norovirus genogroups (GI, GII, and GIV) were detected in effluent, but GIV was never... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15378/12810.pdf |
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| Vaillant, Véronique; Jourdan-da Silva, Nathalie; Quilici, Marie-laure; Couturier, Elisabeth; Le Guyader, Soizick; Delmas, Gilles; Le Saux, Jean-claude. |
| Shellfish filter large volumes of water to meet their nutritional requirements and thus ingest phytoplankton, but also concentrate microorganisms naturally present in water (vibrios, phycotoxins) or of fecal origin (enteric viruses, enterobacteria, protozoan). The analysis of data from systems contributing to the surveillance of foodborne illnesses associated with shellfish consumption and from published outbreak investigations shows that, in France, foodborne illnesses associated with shellfish consumption are mainly of viral origin, mostly due to norovirus followed by hepatitis A virus. The risk linked to phycotoxins appears low and the one related to enterobacteria and vibrios very low. The mandatory notification (MN) of foodborne outbreaks remains the... |
| Tipo: Text |
Palavras-chave: Toxi-infections alimentaires; Coquillages; Phycotoxines; Risques biologiques; Surveillance épidémiologique; Déclaration obligatoire; France; Foodborne illnesses; Shellfish; Phycotoxins; Biological risks; Epidemiological surveillance; Mandatory notification; France. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00092/20362/18023.pdf |
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| Registros recuperados: 97 | |
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