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| Registros recuperados: 40 | |
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| Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele. |
| During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "old" and 2006 for "modern". In total, 84 samples were obtained. Each experimental... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00485/59662/62721.pdf |
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| Lepinay, Alexandra; Turpin, Vincent; Mondeguer, Florence; Grandet-marchant, Quentin; Capiaux, Herve; Baron, Regis; Lebeau, Thierry. |
| A first insight on the interactions between bacteria isolated from monospecific cultures of the marine diatom Haslea ostrearia and this microalgae is described in this work. From 28 bacterial isolates, the maximal growth rate and the maximal cell concentration were on average 58% and 27% higher (max. 67% for isolate B22). Isolates B5, B10 (both Saccharospirillum sp.) and B22 (Rhodobacter sp.) were the most efficient. The geographical origin of H. ostrearia from which bacteria were isolated had no impact on the algal growth performances, and a given bacterial isolate led to the same algal growth irrespective of the geographical origin of H. ostrearia. To explain the results obtained, a comprehensive integrative strategy was conducted that analyzed the... |
| Tipo: Text |
Palavras-chave: Bacterial selection; Plant-growth-promoting bacteria; Phycosphere; Metabolomics. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54036/55340.pdf |
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| Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp. |
| The bioactivity of phytoplankton toxins that accumulate in shellfish is almost always tested on animal model (mice assay). In spite of these advantages, the ability of this test to explain the nature of the bioactivity remains limited. Besides, the current sanitary control based on targeted methods of identification and quantification (LC-MS/MS) of known toxins, do not permit to detect unknown toxins. In order to cope with this need of identification of unknown toxic substances, a next approach based on global and differential metabolomic profiling was proposed. The first results obtained from extracts that have shown a positive toxicity in mice without the substances potentially responsible for these toxic effects have been measured by targeted methods,... |
| Tipo: Text |
Palavras-chave: Métabolomique; LC-HRMS; Biotoxines marines; Biomarqueur. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00077/18784/16352.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf |
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| Haure, Joel; Hussenot, Jerome; Buzin, Florence; Dupuy, Beatrice; Palvadeau, Hubert; Penisson, Christian; Papin, Mathias; Nourry, Max; Lassus, Patrick; Marcaillou-le Baut, Claire; Mondeguer, Florence; Royer, F.; Amzil, Zouher; Belin, Catherine; Cardinal, Mireille; Le Grel, L.; Le Bihan, V.; Jaouen, P.; Massé, A.; Castaing, Jean-baptiste; Sabiri, Ne.; Pontié, M.. |
| La contamination des denrées d’origine marine par des toxines algales (phycotoxines) a pris depuis quelques années une importance considérable. Ce phénomène est largement lié à l’augmentation du nombre de zones géographiques touchées ainsi qu’à la diversification des espèces toxinogènes et à l’identification de nouveaux composés toxiques. Les données épidémiologiques sont cependant encore peu nombreuses car elles sont liées à la mise en place de contrôles systématiques récents. L’Union Européenne a établi un Laboratoire Communautaire de référence pour les phycotoxines, situé à Vigo (Espagne) qui coordonne les activités des laboratoires Nationaux de références correspondants (en France c’est le LNR Phycotoxines de l’AFSSA qui assure cette mission). C’est... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00092/20277/17922.pdf |
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| Fux, E; Marcaillou-le Baut, Claire; Mondeguer, Florence; Bire, R; Hess, Philipp. |
| It has been demonstrated that polymeric resins can be used as receiving phase in passive samplers designed for the detection of lipophilic marine toxins at sea and was referred to as solid phase adsorption toxin tracking (SPATT). The present study describes the uptake and desorption behaviour of the lipophilic marine toxins okadaic acid (OA) and dinophysistoxin-1 (DTX1) from Prorocentrum lima cultures by five styrene-divinylbenzene based polymeric resins Sepabeads (R) SP850, Sepabeads (R) SP825L, Amberlite (R) XAD4, Dowex (R) Optipore (R) L-493 and Diaion (R) HP-20. All resins accumulated OA and DTX1 from the P. lima culture with differences in adsorption rate and equilibrium rate. Following statistical evaluation, HP-20, SP850 and SP825L demonstrated... |
| Tipo: Text |
Palavras-chave: Polymeric resin; Dinophysistoxin 1; Okadaic acid; SPATT; Prorocentrum lima. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4739.pdf |
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| Mondeguer, Florence; Sibat, Manoella; Reubrecht, Sébastien; Amzil, Zouher; Bormans, Myriam; Hess, Philipp; Briand, Enora. |
| Cyanobacterial proliferation is one of the most harmful hazards, in both freshwater and marine ecosystems. Cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. By integrating LC-MS/MS molecular networking and an innovative experimental design, we... |
| Tipo: Text |
Palavras-chave: Cyanobacteria; Cyanotoxins; Plankton interactions; Co_culture; LC_HRMS; Molecular Network; Metabolomics. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00405/51666/52215.pdf |
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| Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi. |
| Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés..... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf |
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| Poirier, Laurence; Amiard, Jean-claude; Mondeguer, Florence; Quiniou, Francoise; Ruiz, Nicolas; Pouchus, Yves Francois; Montagu, Monique. |
| Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found. |
| Tipo: Text |
Palavras-chave: Matched calibration; Matrix; Electrospray ionization ion trap mass spectrometry ESI IT MS; Marine fungal contamination; Trichoderma sp.; Fungal peptide metabolites. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2990.pdf |
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| Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp. |
| La bioactivité des toxines du phytoplancton qui s’accumulent dans les coquillages est presque toujours évaluée sur modèle animal (test souris). Malgré ses avantages, la capacité de ce test à expliquer la nature de cette bio-activité reste limitée. Par ailleurs, le contrôle sanitaire actuellement basé sur une méthode ciblée d’identification et de quantification d’un ensemble de toxines connues ne permet pas de détecter des toxines encore inconnues. Afin de répondre à ce besoin de caractérisation de substances toxiques inconnues, une nouvelle approche de profilage chimique différentiel et non ciblé, de type métabolomique, a été proposée. Les 2 extraits sélectionnés sont ceux ayant montré une toxicité positive chez la souris, sans que les substances... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00179/29042/27480.pdf |
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| Guitton, Yann; Mondeguer, Florence; Nicolau, Elodie; Royer, Florence; Hilbert, Jean-louis; Fournier, Isabelle; Wisztorski, Maxence. |
| MALDI Mass Spectrometry Imaging (MALDI-MSI) is a two-dimensional MALDI mass spectrometric technique used to visualize the spatial distribution of a large variety of biological molecules (from small metabolites to large proteins) without extraction, purification, separation or labelling of biological samples. Since its introduction by Caprioli et al in 1997, it has become one of the most important molecular histology methods for biomarker hunting and for understanding the spatial distribution of biomolecules in various tissues. MALDI-MSI is thus ideal for complementing the expanding field of metabolomics. Despite the fact that MALDI-MSI have been very successfully applied in divers studies (even in clinical applications) its use remains far from routine,... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00179/29041/27479.pdf |
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| Mohamed Benkada, M; Montagu, M; Biard, J; Mondeguer, Florence; Verite, P; Dalgalarrondo, M; Bissett, J; Pouchus, Y. |
| The production of peptaibols by a marine-related Trichoderma longibrachiatum strain was studied using electrospray ionisation multiple-stage ion trap mass spectrometry (ESI-MSn-IT) and gas chromatography/electron impact mass spectrometry (GC/EI-MS). Two major groups of peptaibols were identified, those with long sequences (20 amino acids) and others with short sequences (11 amino acids). This paper describes the methodology used to establish the sequences of short peptaibols in a mixture without previous individual separation. Nine peptaibols were identified. Among them, eight are new, namely as trichobrachin A I-IV (Aib(9)-Pro(10) sequence) and as trichobrachin B I-IV (Val(9)-Pro(10) sequence). Original Pro(6)-Val(7) and Val(9)-Pro(10) sequences have to... |
| Tipo: Text |
Palavras-chave: Trichoderma longibrachiatum; Marine fungi; Marine products; Electrospray ionisation multiple stage mass spectrometry ion trap (ESI MSn IT); Short peptaibols. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1550.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf |
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| Poyer, Salome; Loutelier-bourhis, Corinne; Coadou, Gael; Mondeguer, Florence; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos. |
| The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX... |
| Tipo: Text |
Palavras-chave: Saxitoxin; Ion mobility-mass spectrometry; Isomers; Multidimensional separation; HILIC. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00249/36067/34924.pdf |
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| Mondeguer, Florence. |
| Metabolomics is a new approach to assess the contamination of organisms, environmental compartments or foodstuffs, and the impact marine pollution, (natural or anthropogenic), can have on them. Through the analysis of the variation of the metabolite profiles present in the cell, tissues, organs or even the whole organism it is possible to define a physiological state, a stage of development, or the level and nature of a contamination. Generally, analysis of metabolites correlates to experimental design built around a clear biological or environmental question, needs to be supported by sensitive and reproducible tools, and requires access to libraries "house" or international. Often, the non-targeted approach allows for evaluation of many hypotheses. Thus,... |
| Tipo: Text |
Palavras-chave: Biotoxines; Biomarqueurs; Métabolomique; Microalgues; Moules; Spectrométrie de masse haute résolution (HRMS); Workflow. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00358/46892/46777.pdf |
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| Briand, Enora; Reubrech, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam. |
| Freshwater cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. We tested if the production of a range of peptides by co-existing species could be regulated through interspecific interactions. Using a combined approach of co-cultures and analyses... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00374/48515/48794.pdf |
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| Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois. |
| Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques... |
| Tipo: Text |
Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf |
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| Registros recuperados: 40 | |
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