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| Registros recuperados: 12 | |
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| Fogaça,Cláudia Martellet; Sant' Anna-Santos,Bruno Francisco; Cordeiro,Delaine Correia; Correia,Teresa Drummond; Finger,Fernando Luiz; Otoni,Wagner Campos; Cargnin,Adeliano. |
| Objetivou-se induzir o processo de microtuberização in vitro de cultivares de mandioca (Manihot esculenta Crantz.), em dois tipos de meio, sob a ação de diferentes reguladores de crescimento e concentrações de sacarose, e verificar as alterações estruturais decorrentes do processo de tuberização. Foram analisadas a influência do tipo de meio, a ação do BAP e ANA e doses de sacarose (3%, 6% e 8% p/v) através das características comprimento da parte aérea, o número médio de raízes, o padrão morfológico das raízes tuberiformes, bem como características anatômicas. Verifica-se que as cultivares de mandioca respondem diferentemente à microtuberização in vitro. A cultivar Parazinha responde melhor à indução de raízes tuberiformes, tendo o tratamento constituído... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta (Crantz); Cultura in vitro; Regulador de crescimento; Tuberização. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000300004 |
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| Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos. |
| The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001 |
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| Batista,Diego Silva; Dias,Leonardo Lucas Carnevalli; Rêgo,Mailson Monteiro do; Saldanha,Cleber Witt; Otoni,Wagner Campos. |
| ABSTRACT: The influence of flask sealing and explant source on the in vitro morphogenesis of pepper, were evaluated in Capsicum annuum. Seeds were sterilized and inoculated in Murashige and Skoog media supplemented with vitamins, myo-inositol, sucrose, and agar. Gas exchange was evaluated in the germination stage by comparing 3 flask-sealing systems: rigid polypropylene lids (PLs) without vents, PLs with 1 vent, and PLs with 2 vents covered with membranes. In the regeneration stage, cotyledon and hypocotyl segments were transferred to the organogenesis-inducing media, being the different sealing types also tested in a factorial scheme. Photosynthetic pigments, morphological and hystological analyses were conducted for each treatment. Plants maintained in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Gas exchange; Ornamental plant; Capsicum annuum. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300402 |
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| Oliveira,Maria Luiza Peixoto de; Costa,Marcio Gilberto Cardoso; Silva,Crislene Viana da; Otoni,Wagner Campos. |
| The objective of this study was to evaluate the effects of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) combinations, basal media and beta-lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige and Skoog (MS) medium containing the following BAP/NAA concentrations: 0.0/0.0; 0.25/0.0; 0.25/0.25; 0.5/0.0; 0.5/0.5; 1.0/0.0; 2.0/0.0; 2.0/0.25; 2.0/0.5; and 2.0/1.0 mg L-1. In order to test the influence of the culture media on shoot-bud induction, (MS), Murashige and Tucker (MT), and woody plant medium (WPM) formulations were evaluated, associated with the best... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus limonia; C. sinensis; Adult tissues; Antibiotics; Organogenesis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700004 |
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| Alves,Elisa Cristina Soares de Carvalho; Xavier,Aloísio; Otoni,Wagner Campos. |
| O objetivo deste trabalho foi avaliar os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tia-diazol-5-il)uréia], BAP (6-benzilaminopurina) e ANA (ácido naftalenoacético) no desempenho da propagação in vitro por organogênese de explante foliar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla. Houve resposta diferenciada dos clones quanto a intensidade, textura e coloração dos calos, em razão dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento dos três genótipos foram observados nos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1) e ANA (0,1 mg L-1), obtendo-se 100% de calejamento no explante foliar. Os piores resultados de calejamento foram observados nos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagação; Reguladores de crescimento; Propagação in vitro. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000500003 |
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| Moreira,Bruno Coutinho; Prates Junior,Paulo; Jordão,Thuany Cerqueira; Silva,Marliane de Cássia Soares da; Ribeiro,Ana Paula Ferreira; Stürmer,Sidney Luiz; Salomão,Luiz Carlos Chamhum; Otoni,Wagner Campos; Kasuya,Maria Catarina Megumi. |
| ABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative to supply the demand for the production of glomerospores. This study aimed to multiply and evaluate AMF inoculum originating from isolated species (including Rhizophagus clarus, Claroideoglomus etunicatum) versus native AMF from pineapple and coffee plantations multiplied by the on-farm method on the colonization in pineapple plantlets. Initially,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycorrhizal inoculant; Spore viability; AMF; Rhizophagus clarus; Claroideoglomus etunicatum. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832019000100409 |
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| Alexandre,Rodrigo Sobreira; Lopes,José Carlos; Tiradentes,Aristhotelis Tadeu; Bruckner,Claudio Horst; Otoni,Wagner Campos. |
| O objetivo foi avaliar a metodologia de enxertia pela modalidade garfagem no topo hipocotiledonar em fenda cheia, utilizando miniestacas adultas de segmentos apicais de P. edulis f. flavicarpa, P. edulis, P. alata, P. mucronata e P. foetida e internodais de P. mucronata. O experimento foi conduzido em casa de vegetação equipada com sistema de irrigação com nebulização intermitente, do Centro Universitário Norte do Espírito Santo (CEUNES), da Universidade Federal do Espírito Santo (UFES). Os índices de pegamento nas combinações de P. edulis f. flavicarpa/P. edulis f. flavicarpa, P. edulis/P. edulis f. flavicarpa, P. alata/P. edulis f. flavicarpa, P. mucronata/P. edulis f. flavicarpa, P. foetida/P. edulis f. flavicarpa foram de 90; 90; 68; 80 e 90,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora spp; Propagação; Garfagem. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000100040 |
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| Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner Campos. |
| Abstract The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seed germination; Vegetative propagation; Cuttings; Grafting; Tissue culture. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201002 |
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| Registros recuperados: 12 | |
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