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| Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro. |
| Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of... |
| Tipo: Journal article |
Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009 |
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| Castagnaro,Atilio Pedro; Filippone,María Paula; Noguera,Aldo Sergio; Perera,María Francisca; Vellicce,Gabriel Ricardo; Mamaní,Alicia Inés; Ontivero,Marta Inés; Arias,Marta Eugenia; Racedo,Josefina; García,María Gabriela; Paz,Nora; Díaz,María Elena; Sendín,Lorena Noelia; Pardo,Esteban Mariano; Orce,Ingrid Georgina; Rocha,Carla María Lourdes; Sepúlveda Tusek,Mlena; Martínez Zamora,Gustavo; Salazar,Sergio Miguel; Dantur,Karina Inés; Rigano,Luciano; Torres,Pablo; Chalfoun,Nadia Regina; Maraño,María Rosa; Vojnov,Adrián; Ploper,Leonardo Daniel; Díaz Ricci,Juan Carlos. |
| En 1994 se conformó un grupo de investigación científica y desarrollo tecnológico guiado por la demanda del sector agroindustrial de la región del noroeste argentino, que además de las consideraciones económicas intrínsecas del proceso productivo, tuvo en cuenta para sus investigaciones, aspectos sociales y ambientales. Las premisas conceptuales del trabajo científico fueron el estudio, la preservación y el aprovechamiento de la biodiversidad, en conjunción con la búsqueda continua de alternativas de manejo fitosanitario lo menos reñidas posible con la salud humana y ambiental. Fue así que en 1997 se inició el primer Programa Nacional de Mejoramiento Genético de la Frutilla en Argentina, que desde el INSIBIO (CONICET-UNT) en Tucumán coordinó el trabajo de... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bioproductos; Caña de azúcar; Citrus; Cultivo de tejidos y micropropagación; EEAOC; Frutilla; INSIBIO; Marcadores moleculares; Mejoramiento genético molecular; Soja; Transformación de plantas. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000100016 |
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| Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro. |
| ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; RDNA; SSR; Infection courts. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200 |
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| La O,María; Perera,María Francisca; Bertani,Romina Priscila; Acevedo,Ricardo; Arias,Marta Eugenia; Casas,Mario Alberto; Pérez,Juana; Puchades,Yaquelín; Rodríguez,Eida; Alfonso,Isabel; Castagnaro,Atilio Pedro. |
| ABSTRACT: Multiple pathogens affect sugarcane, among them Puccinia melanocephala, the causal agent of brown rust. This disease was first reported in Cuba in 1979 when it was responsible for a severe attack on the main sugarcane variety B4362. The aims of the present study were to give an overview of sugarcane brown rust in Cuba and show the current disease situation in the country. A retrospective analysis regard to sugarcane cultivar composition resistant to brown rust in Cuba was carried out. In addition 154 genotypes, including the most used progenitors in the breeding program and commercial varieties were evaluated under natural infection conditions. The identity of P. melanocephala was verified by Polymerase Chain Reaction (PCR) and by sequencing the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Puccinia melanocephala; Varietal mosaic; Resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300233 |
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