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| Registros recuperados: 39 | |
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| Zemani, Faouzia; Benisvy, Danielle; Galy Fauroux, Isabelle; Lokajczyk, Anna; Colliec-jouault, Sylvia; Uzan, Georges; Fischer, Anne Marie; Boisson Vidal, Catherine. |
| Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the... |
| Tipo: Text |
Palavras-chave: Fucoidan; FGF 2; Endothelial progenitor cells; Angiogenesis; Alpha 6. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-679.pdf |
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| Boisson-vidal, C; Zemani, F; Calliguiri, G; Galy-fauroux, I; Colliec-jouault, Sylvia; Helley, D; Fischer, A-m. |
| Fucoidans -- sulphated polysaccharides extracted from brown algae - could be beneficial in patients with ischemic diseases. Their antithrombotic and proangiogenic properties promote in animals, neovascularization and angiogenesis which prevent necrosis of ischemic tissue. In 1997, endothelial progenitor cells were first identified in human peripheral blood. They are recruited from bone marrow and contribute to neovascularization after ischemic injury. Mobilization of these cells in ischemic sites is an important step in new vessel formation. It is thought that the progenitors interact with endothelial cells, then extravasate and reach ischemic sites, where they proliferate and differentiate into new blood vessels. Although chemokines, cytokines and... |
| Tipo: Text |
Palavras-chave: Growth factors; Cytokines; Angiogenesis; Neovascularization; Endothelial progenitor cells; Sulphated polysaccharides; Fucoidan. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2168.pdf |
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| Sarlon, G.; Zemani, F.; David, L.; Van Huyen, J. -p. Duong; Dizier, B.; Grelac, F.; Colliec-jouault, Sylvia; Galy-fauroux, Isabelle; Bruneval, P.; Fischer, Anne-marie; Emmerich, J.; Boisson-vidal, Catherine. |
| . Background: Fucoidan, an antithrombotic polysaccharide, can induce endothelial colony-forming cells (ECFC) to adopt an angiogenic phenotype in vitro. Objectives: We evaluated the effect of fucoidan on vasculogenesis induced by ECFC in vivo. Methods: We used a murine hindlimb ischemia model to probe the synergic role of fucoidan-treatment and ECFC infusion during tissue repair. Results: We found that exposure of ECFC to fucoidan prior to their intravenous injection improved residual muscle blood flow and increased collateral vessel formation. Necrosis of ischemic tissue was significantly reduced on day 14, to 12.1% of the gastronecmius cross-sectional surface area compared with 40.1% in animals injected with untreated-ECFC. ECFC stimulation with fucoidan... |
| Tipo: Text |
Palavras-chave: Angiogenesis; Endothelial colony-forming cells; Fucoidan; Ischemia; Revascularization. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00065/17616/18204.pdf |
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| Bouvard, Claire; Galy-fauroux, Isabelle; Grelac, Francoise; Carpentier, Wassila; Lokajczyk, Anna; Gandrille, Sophie; Colliec-jouault, Sylvia; Fischer, Anne-marie; Helley, Dominique. |
| Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were... |
| Tipo: Text |
Palavras-chave: Fucoidan; Angiogenesis; Vasculogenesis; Migration; Signaling; Transcriptomics. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00308/41913/41187.pdf |
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| Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique. |
| The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)... |
| Tipo: Text |
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf |
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| Roa,Ignacio; Cantín,Mario; Vilos,Cristian; Rosas,Carlos; Lemus,David. |
| Although, antineoplastic therapies have now been developed reduction of tumor progression,itis necessarytofind new therapeutic alternatives to suppress angiogenesis.Thus celecoxib (Cx) has been used for its antiangiogenic action in combination with certain polymeric compounds such as poly (lactic co-glycolic acid) (PLGA) acid, which help to improve the bioavailability and avoid effects of long drug administrations. For this purpose we used a murine tumor modelinduced by mammary adenocarcinoma cells resistant to chemotherapy (TA3-MTXR). CX/PLGA inhibits the microvascular density, VEGF expression and cell proliferationinaddition to increased apoptosis (P <0.0001). Cx reduces tumor progression in a concentration of 1000 ppm associated with PLGA, reducing... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Cancer; Celecoxib; PLGA; Apoptosis; VEGF; CellProliferation. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200055 |
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| Rádis-Baptista,G.. |
| Integrins encompass a family of transmembrane heterodimeric proteins of adhesion that maintain cells attached to other cells and to the extracellular matrix (ECM). Integrins work as bi-directional mechanotransducers, conveying mechanical signal from outside to inside the cell through a cascade of phosphorylation signals. On the other hand, the signal from inside to outside controls the strength and affinity of integrin adhesion. As proteins of focal contact, integrins are involved in diverse cell functions, such as cell activation, migration, growth, and survival. In the development of neoplastic disease and metastatic tumor, integrins can influence cancer invasiveness and progression, as well as mediate the formation of new blood vessels (angiogenesis).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Integrin; Angiogenesis; Cancer; Apoptosis; Snake toxin; Snake venom C-type lectin; Metalloprotease; Disintegrin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300002 |
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| SHIMIZU, Takashi; IIJIMA, Koji; SASADA, Hiroshi; SATO, Eimei; 清水, 隆. |
| Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histological observation of the capillary network. Granulosa cells and thecal tissues in small (<4 mm), medium (4-5 mm) or large (>5 mm) individual follicles were collected for detection of mRNA expression of HGF, Ang-1 and Ang-2 in granulosa cells, and HGF receptor... |
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Palavras-chave: Follicular Development; Angiogenesis; Angiopoietins; HGF; Pig. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4055 |
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| Barros,Luiz F.M.; Belfort-Jr,Rubens. |
| The purpose of this study was to evaluate the effects of the use of the subconjunctival injection of bevacizumab (Avastin®) on angiogenesis in the rat cornea. Corneas of 20 Wistar male rats were cauterized with silver nitrate crystal. Animals were divided in four groups: control group (GC) that received subconjunctivally 0.02 ml of 0.9% saline solution on the day of the lesion; group GO that received subconjunctivally 0.02 ml of bevacizumab just after the lesion; group G3 that received bevacizumab on day 3 and group G5 that received bevacizumab on day 5 after lesion. Animals were euthanized on day 7. The newly formed vessels were quantified after China Ink perfusion and photographs were obtained and analyzed in a computerized system (Image Pro-Plus®). In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bevacizumab; Cornea; Neovascularization; Angiogenesis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000300004 |
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| Santos,G.J.L.; Ferreira,T.C.; Rodrigues,A.L.M.; Freitas,J.C.C.; Morais,S.M.; Girão,V.C.C.; Nunes-Pinheiro,D.C.S.. |
| ABSTRACT This study aimed to evaluate Himatanthus drasticus latex in a mice wound healing experimental model. Animals were divided into four groups (n=7) according to the treatments: GI - saline 0.9% (control), GII - mineral oil (vehicle), GIII - H. drasticus commercial latex (HdCL) and GIV - H. drasticus mixed isolated fraction (MIF, 1 mg/mL). The treatments were applied topically once daily, 50 µL for 14 consecutive days. Macroscopic lesions were evaluated, considering parameters such as swelling, redness, granulation tissue and reepithelialization. VEGF+, CD68+ expressions and mast cells (Toluidin blue stain) were evaluated. HdCL induced higher contraction and exuberant granulation tissue (P > 0.05). HdCL showed a mild inflammatory process while MIF... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Latex; Apocinaceae; Wound healing; Angiogenesis; Inflammatory infiltrate. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300513 |
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| Souza,C; Carvalho,L.F.; Ferreira,M.A.N.D; Pesquero,J.L.; Cassali,G.D. |
| A proteína Kint3-4 (PKint3-4), codificadora da angiostatina, é reconhecida por sua potencialidade antiangiogênica. O presente estudo teve como objetivo avaliar o efeito da proteína Kint3-4 no crescimento do tumor sólido de Ehrlich. Para isso, foram analisados a curva de desenvolvimento tumoral, o índice apoptótico e a dosagem de hemoglobina, a fim de se avaliar a angiogênese, em 20 camundongos Swiss fêmeas, inoculadas com o tumor sólido de Ehrlich em seus coxins plantares. Os resultados demonstraram a participação de PKint3-4 na indução à apoptose de células neoplásicas, na diminuição da concentração de hemoglobina e, principalmente, na diminuição do desenvolvimento tumoral. Sugere-se que a ação antitumoral, determinada pela sequência proteica utilizada,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Camundongo; Tumor de Ehrlich; Kint3-4; Apoptose; Angiogênese; Caspase-3; Tumor de Ehrlich; Mice; Ehrlich tumor; Kint3-4; Apoptosis; Angiogenesis; Caspase-3. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300036 |
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| Pazzini,J.M.; Serafim,E.L.; Uscategui,R.R.A.; Almeida,V.T.; Oliva,C.A.C.; Gärtner,F.; Amorim,I.; Faria,F.; Rêma,A.; Reis Filho,N.P.; Ferreira,M.G.P.A.; Silva,A.C.; Huppes,R.R.; Moraes,P.C.; Oliveira,J.A.; Nardi,A.B.. |
| ABSTRACT The aim of this study was to evaluate of the efficacy of PRP employment associated with surgical sponges to improve the integration of the graft in the recipient bed. It was held at the Veterinary Hospital UNESP, Campus of Jaboticabal - SP, a study of 64 rabbits, divided into eight groups with eight animals. The groups were divided in control with saline solution 0,9%, control with PRP both without the sponge, surgical sponge with PRP, surgical sponge without PRP, and were used mesh and layer grafts in the respective groups. The data were submitted to statistical analysis (paired t-test, Kruskal-Wallis test, with subsequent use of the multiple comparison tests of Dunn, analysis of variance (F) test, Tukey test, P< 0.05). Edema and exudate with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabbit; Skin grafts; Compressive dressing; Angiogenesis; PRP. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501339 |
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| Rosas,Carlos; Sinning,Mariana; Ferreira,Arturo; Fuenzalida,Marcela; Lemus,David. |
| BACKGROUND: During the last few years it has been shown in several laboratories that Celecoxib (Cx), a non-steroidal anti-inflammatory agent (NSAID) normally used for pain and arthritis, mediates antitumor and antiangiogenic effects. However, the effects of this drug on a tumor cell line resistant to chemotherapeutical drugs used in cancer have not been described. Herein we evaluate the angiogenic and antitumor effects of Cx in the development of a drug-resistant mammary adenocarcinoma tumor (TA3-MTXR). RESULTS: Cx reduces angiogenesis in the chick embryonic chorioallantoic membrane assay (CAM), inhibits the growth and microvascular density of the murine TA3-MTXR tumor, reduces microvascular density of tumor metastases, promotes apoptosis and reduces... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Celecoxib; Tumor. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100027 |
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| ARANDA,EVELYN; OWEN,GARETH I. |
| Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; EA.hy926; HUVEC; Endothelial; Formula; In vitro assay; Matrigel. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012 |
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| Garrido,Osvaldo; Letelier,René; Rosas,Carlos; Fuenzalida,Marcela; Ferreira,Arturo; Lemus,David. |
| Tumor resistance to traditional cancer treatments poses an important challenge to modern science. Thus, angiogenesis inhibition is an important emerging cancer treatment. Many drugs are tested and corticosteroids have shown interesting results. Herein we investigate the effect on microvessel density, survival time and tumoral volume of mice with TA3-MTX-R tumors. Twenty six mice were inoculated with lxlO6 tumor cells, 4-5 days after injection, six mice were injected with PBS (group A) and twenty mice were treated with p-met (group B). All animals from Group A died on day 22. Group B was divided into Bl (treated discontinued) and B2 (treated daily) and observed until day 88. All mice were processed for histo-immunohistochemical analysis and the blood... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Betamethasone treatment; Metastasis. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300008 |
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| ILLANES,JULIO; DABANCENS,ALFREDO; ACUÑA,OLGA; FUENZALIDA,MARCELA; GUERRERO,ANIBAL; LOPEZ,CLAUDIA; LEMUS,DAVID. |
| In this study, we showed the effect of the betamethasone, sulindac and quinacrine alone or combined, on the inflammatory angiogenesis promoted by polyurethane sponge on mice. The main finding reported here is that the formation of new blood vessels was strongly inhibited by low concentration of betamethasone, sulindac or quinacrine, whether alone or in combination. It is known that steroidal anti-inflammatory drugs inhibit the enzymes required for the production of prostaglandins through a nuclear glucocorticoid receptor (GR) mediated mechanism. This mechanism may occur in endothelial cells as well. Considering that activity of cyclo-oxigenases 1 and 2 is inhibited by sulindac, and that these enzymes are located in the stromal tissue, we propose that the... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Antiangiogenesis; Betamethasone; Sulindac; Quinacrine. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300008 |
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| Registros recuperados: 39 | |
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