SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Different swine production systems can shape slurry resistome at mechanism and class levels based on swine manure evaluation.

DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B..

Abstract The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway...

Tipo: Artigo de periódico

Palavras-chave: Antimicrobial resistance; Coagulase-negative staphylococci; Methicillin-resistant S aureus; PFGE; Staphylococci.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118368

Detection of biofilm-forming Staphylococcus aureus isolated from bovine mastitis and association with antimicrobial resistance in Brazil.

BELTRAME, L. C.; ZAMPARETTE, C. P.; FELTRIN, C.; CUNHA, C. R. da; COLTRO, E. P.; ATHAYDE, G. S. da S.; BENETTI FILHO, V.; TÁPPARO, D. C.; MONTEIRO, J.; KICH, J. D.; PALMEIRO, J. K.; WAGNER, G.; FONGARO, G.; ZÁRATE-BLADÉS, C. R.; SINCERO, T. C. M..

Tipo: Artigo de periódico

Palavras-chave: Resistência antimicrobiana; Resistência aos antibióticos; Sequenciamento; Metagênomica; Resistoma; Antimicrobial resistance; MinION nanopore device®; Swine manure; NGS-next-generation sequencing; Chorume; Suíno; Dejeto; Esterco; Sus Domesticus; Animal husbandry; Pig manure; Metagenomics; One Health initiative.

Ano: 2022

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144728

Risk factors associated with the antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis.

PÉREZ, V. K. C.; MAGOSSI, E. M.; OLIVEIRA, P. F. R. e; CUSTODIO, D. A. C.; CRUZ, H. F. DA; ABREU, J. P. DE; HEINEMANN, M. B.; GUIMARÃES, A. S.; DORNELES, E. M..

Tipo: Separatas

Palavras-chave: Antimicrobial resistance; Slime-producing; Bovine mastitis.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104635

Antimicrobial resistance on Staphylococcus aureus isolated from cases subclinical mastitis in goats.

BEURON, D. C.; CORTINHAS, C. S.; BOTARO, B. G.; MACEDO, S. N.; GONÇALVES, J. L.; BRITO, M. A. V. P. e; SANTOS, M. V..

Tipo: Separatas

Palavras-chave: Antimicrobial resistance; Treatment; Management practices; In vitro susceptibility.; Staphylococcus Aureus.; Mastitis; Risk factors..

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1014001

Antimicrobial resistance profile of Staphylococcus aureus isolated in goat milk.

MENEZES, L. M. F; ÂNGELO, F. F.; ALCINDO, J. F.; SÁ, D. M. A.; LIMA, A. R.; ARAUJO, R. F.; MESQUITA, F. L. T.; SOUZA, V. de.

Caprine mastitis is one of the most common diseases in dairy herds, causing damage mainly due to the decrease in milk production after the invasion of the infectious agent in the secretory epithelium of the mammary gland. Species Staphylococcus aureus is the most pathogenic agent in the mammary gland, having important implications for public health, since it produces enterotoxins that survive the heat treatments applied to milk. The use of proper antibiotics is an important tool for the treatment of the disease. However, they should be used judiciously, since the main cause of failure in therapy is bacterial resistance to the drug. This study uses an antibiogram to evaluate the resistance of microorganisms isolated from goat milk samples with subclinical...

Tipo: Anais e Proceedings de eventos

Palavras-chave: Resistance to antibiotics; Resistance to chemicals; Antimicrobial resistance; Tratamento químico; Resistência a medicamento; Mamite; Caprino; Cabra Leiteira; Doença Animal; Antibiótico; Resistência Química; Goat diseases; Goat milk; Chemical treatment; Public health; Drug resistance.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114014

Pulsed-field gel electrophoresis and antimicrobial resistance profiles of Campylobacter jejuni isolated from Brazilian broiler farms.

ÂNGELO, F. F.; SCHWAAB, R. D. C.; REZENDE, A. P. M.; LOPES, M. F.; AGUIAR, D. M.; SOUZA, V. de; MELO JUNIOR, A. M.; FERREIRA, M. H..

Abstract: The present work aimed to study the antimicrobial resistance profile of Staphylococcus aureus isolated from goal milk. Two hundred and twelve samples of goat milk were used for the microbiological analyses. In ten (4.7%) of these samples, strains of Staphylococcus aureus were isolated. The susceptibility of the strains of S. aureus was assayed by the disc diffusion method. The antibiotics tested were: Ampicillin-10?g (AMP), Chloramphenicol-30?g (CHL), Tetracycline-30?g (TET), Gentamicin-10?g (GEN), Trimethoprim-Sulfamethoxazole-25?g (SXT), Erythromycin-30?g (ERY), Cefoxitin-30?g (CFO), Ciprofloxacin-05?g (CIP), Penicillin-10? (PEN)), Clindamycin-02?g (CLI), Azithromycin-15?g (AZM), Rifampicin-05?g (RIF) and Linezolid 30?g (LZD). The resistance of...

Tipo: Anais e Proceedings de eventos

Palavras-chave: Antimicrobial sensitivity; Antimicrobial resistance; Resistance to chemicals; Antimicrobiano; Caprino; Cabra Leiteira; Doença Animal; Mamite; Bactéria; Dairy goats; Bacterial infections; Mastitis.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113856

Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure.

VAZ, C. S. L.; RECH, D. V.; LOPES, L. dos S.; SILVA, V. S..

We studied the on-farm variety of thermotolerant Campylobacter strains isolated from field samples received over a 12-mo period from four broiler-producing companies in southern Brazil. In total, 515 of 807 (63.8%) of analyzed samples were found to be Campylobacter-positive. Campylobacter jejuni and C. coli were detected; the first one being more frequently isolated (98.2%). The highest antimicrobial resistance levels in a subset of 80 C. jejuni strains were found against enrofloxacin (89.5%) and ciprofloxacin (83.3%). Most of the 28 DNA macrorestriction profiles identified in C. jejuni were unique to each sampled broiler company. Genetically related C. jejuni populations were detected on different broiler farms integrated to the same company on different...

Tipo: Artigo de periódico

Palavras-chave: Resistência antimicrobiana; Antimicrobial resistance; Ave Domestica; Análise Microbiológica; Poultry; Campylobacter jejuni.

Ano: 2021

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133195

Surveillance programs for detection and characterization of emergent pathogens and antimicrobial resistance: results from the Division of Infectious Diseases, UNIFESP

RESENDE, J. A.; SILVA, V. L.; OLIVEIRA, T. L. R. de; FORTUNATO, S. de O.; CARNEIRO, J. da C.; OTENIO, M. H.; DINIZ, C. G..

Tipo: Separatas

Palavras-chave: Biogas Biofertilizer; Dairy cattle manure; Antimicrobial resistance; Pathogenic bacteria.; Anaerobic digestion..

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/988214

Anais da ABC (AABC)

Colombo,Arnaldo L.; Janini,Mario; Salomão,Reinaldo; Medeiros,Eduardo A. S.; Wey,Sergio B.; Pignatari,Antonio C. C..

Several epidemiological changes have occurred in the pattern of nosocomial and community acquired infectious diseases during the past 25 years. Social and demographic changes possibly related to this phenomenon include a rapid population growth, the increase in urban migration and movement across international borders by tourists and immigrants, alterations in the habitats of animals and arthropods that transmit disease, as well as the raise of patients with impaired host defense abilities. Continuous surveillance programs of emergent pathogens and antimicrobial resistance are warranted for detecting in real time new pathogens, as well as to characterize molecular mechanisms of resistance. In order to become more effective, surveillance programs of...

Phenotypic and genotypic characterization of Salmonella spp. isolated from foods and clinical samples in Brazil

Palavras-chave: Emerging infectious diseases; HIV; AIDS; Candidemia; Antimicrobial resistance; Bacteremia; Sepsis; Nosocomial infectious.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300020

Anais da ABC (AABC)

MIRANDA,ANDREA L.; CORDEIRO,SORAIA M.; REIS,JOICE N.; CARDOSO,LUCAS G.; GUIMARÃES,ALAÍSE G..

ABSTRACT Increasing antimicrobial resistance in Salmonella species has been a serious problem for public health worldwide. This study examines Salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and PFGE genotypes. It identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. Salmonella Infantis (16.5%) and Salmonella Enteritidis (13.7%) are the most common among food isolates, whereas Salmonella Enteritidis (29.0%) and Salmonella Typhimurium (16.0%) mainly causes human salmonellosis. Antimicrobial susceptibility data showed that 63.0% of the isolates were fully susceptible to 12 antibiotics tested. Nalidixic acid showed high resistance rates, 32.7% and...

Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals

Palavras-chave: Salmonella; Salmonella Infantis; Salmonella Enteritidis; Antimicrobial resistance.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301143

Carneiro,V.C.; Lessa,D.A.B.; Guttmann,P.M.; Magalhaes,H.; Aquino,M.H.C.; Cunha,L.E.R.; Arais,L.R.; Cerqueira,A.M.F..

ABSTRACT Respiratory diseases are common in young horses but little is known about such infections in mule foals. This study aimed to characterize Escherichia coli and Klebsiella sp. isolated from tracheal wash (TW) and fecal samples (FS) of mule foals, with or without cytological evidence of respiratory disease. Strains were analyzed against 13 antimicrobials, for presence of Extended spectrum beta-lactamase (ESBL), and virulence genes. Phylogrouping and Randomic (RAPD)-PCR profiles were used to evaluate their genetic relatedness. E. coli strains from TW and FS showed greatest resistance to tetracycline, while Klebsiella strains were mainly resistant to ampicillin; multidrug resistance and ESBL production were also detected. The blaCTX gene prevailed...

Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

Palavras-chave: Escherichia coli; Klebsiella; Respiratory disease; Virulence; Antimicrobial resistance.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501073

Bourguignon,E.; Viçosa,G.N.; Corsini,C.M.M.; Moreira,M.A.S.; Nero,L.A.; Conceição,L.G..

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderma and evaluate their resistance profiles. Lesions from 25 dogs with pyoderma were sampled. Bacterial isolates were subjected to phenotypic and genotypic analysis for identification of the causative agent. S. pseudintermedius isolates were subjected to SmaI macrorestriction analysis and PFGE for genetic grouping, and PCR to identify the presence of the mecA gene. Their resistance profiles against 12 antimicrobials were also assessed. According to the...

Virulence factors, antimicrobial resistance, and plasmid content of Escherichia coli isolated in swine commercial farms

Palavras-chave: Staphylococcus pseudintermedius; Canine pyoderma; MecA; PFGE; Antimicrobial resistance.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200299

Costa,M.M.; Drescher,G.; Maboni,F; Weber,S.S.; Schrank,A.; Vainstein,M.H.; Schrank,I.S.; Vargas,A.C..

Virulence factors and antimicrobial resistance patterns of Escherichia coli isolates were evaluated. A total of 80 E. coli isolates were evaluated, being 64 from clinical samples (intestinal content and fragments of organs from diarrheic piglets), seven from feces of clinically healthy piglets and sows, and nine environmental samples (five from facilities, two from feed, one from insect, and one from waste). Molecular characterization was performed by PCR detection of fimbriae and toxin genes and plasmid content determination. The isolates were also characterized according to their resistance or sensitivity to the following drugs: ampicillin, trimethoprim:sulfamethoxazole, tetracycline, amikacine, colistin, norfloxacin, florfenicol, enrofloxacin,...

Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil

Palavras-chave: Swine; Colibacillosis; Fimbriae; Toxin; Antimicrobial resistance.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000100004

Paneto,B.R.; Schocken-Iturrino,R.P.; Macedo,C.; Santo,E.; Marin,J.M..

The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid...

Resistência bacteriana e ação das bacteriocinas de Lactobacillus spp em Staphylococcus aureus isolados de mastite bovina

Palavras-chave: Escherichia coli; Cheese; VTEC; ETEC; Antimicrobial resistance.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200035

Pribul,B.R.; Pereira,I.A.; Soares,L.C.; Coelho,S.M.O.; Barberis,I.L.; Pascual,L.; Souza,M.M.S..

Staphylococcus aureus is the major pathogen causing intramammary infections in dairy cattle worldwide. Among the factors that contribute to its spread and infectious potential is the ability to overcome the mechanisms of antimicrobials activity. The present work investigated the antimicrobial resistance pattern and sensibility to bacteriocins produced by strains of Lactobacillus spp of 30 isolates of S. aureus from mastitis. From this, 29 are beta-lactamase producers. Eight isolates (26.6%) showed resistance to at least four antibiotics being considered multiresistent. All of them were mecA-positive. Otherwise, all isolates tested showed sensibility to at least one of the four bacteriocin producer strains. Due to the significant depletion of the efficacy...

Antimicrobial susceptibility of coagulase-negative Staphylococci isolated from mastitic cattle in Brazil

Palavras-chave: Staphylococcus aureus; Bovine mastitis; Antimicrobial resistance; Bacteriocins; Lactobacillus spp.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300029

Machado,T.R.O.; Correa,M.G.; Marin,J.M..

Um total de 109 cepas de Staphylococci coagulase-negativa foi isolado de leite de vacas com mastite clínica e subclínica, em 35 fazendas, situadas em nove estados brasileiros, no período de fevereiro a maio de 2005. Os isolados foram investigados em relação a susceptibilidade in vitro a diversos agentes antimicrobianos. A resistência à penicilina foi a observação mais freqüente (93,5%), seguida por sulfonamida (88,9%), novobiocina (88,6%) e ampicilina (85,3%). Todas as cepas examinadas mostraram resistência a pelo menos uma das drogas antimicrobianas testadas. Cepas apresentando resistência múltipla foram extremamente comuns, com 10,0% dos microrganismos isolados apresentando resistência a todas as drogas antimicrobianas. Os resultados obtidos indicaram...

Enterococcus spp. Resistant to Multiple Antimicrobial Drugs and Determination of Fecal Contamination Levels in Mangrove Oysters (Crassostrea rhizophorae)

Palavras-chave: Coagulase-negative Staphylococci; Antimicrobial resistance; Bovine mastitis.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100041

BABT

Rubião,Cynthia Annes; Franco,Robson Maia; Mesquita,Eliana de Fátima Marques de; Miguel,Marco Antonio Lemos; Cabral,Claudius Couto; Fonseca,Ana Beatriz Monteiro.

ABSTRACT The aim of this study was to determine and compare the Most Probable Number (MPN) of Total Coliforms (TC), Escherichia coli and Enterococcus spp. and to characterize the antimicrobial resistance profiles of Enterococcus spp. isolated from oysters collected in the Barra de Guaratiba Mangrove, Rio de Janeiro, Brazil. The enumeration of E. coli has been used to indicate fecal contamination and hygienic-sanitary conditions of bivalve molluscs. Enterococci are capable to transfer several antimicrobial resistance genes to pathogenic bacteria, including those from Gram-negative group. The oysters were bought from local fishermen and a total of 123 individuals were analyzed. The TC, E. coli and Enterococcus spp. MPN mean were 26,300/100 g, 3,260/100 g and...

Evaluation of Medicinal Values of Gymnopetalum chinense (Lour.) Merr., a Lesser Known Cucurbit from Eastern Ghats of India

Palavras-chave: Water pollution; Escherichia coli; Enterococcus spp.; Antimicrobial resistance; Bivalve molluscs.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100308

BABT

Kumar,Sanjeet; Das,Gitishree; Shin,Han-Seung; Kumar,Pradeep; Patra,Jayanta Kumar.

ABSTRACT >The in vitro antimicrobial activity of Gymnopetulum chinense (Cucurbitaceae), a lesser known climber of Eastern Ghats and recently recorded species from Odisha, India was studied against five selected human pathogenic bacteria (Streptococcus mutans, S. pyogenes, Vibrio cholerae, Shigella flexneri and Salmonella typhi), by agar well diffusion (AWD), disc diffusion (DD) and broth dilution methods. Different solvent extracts were prepared from the leaves of G. chinense using various solvents viz. n-hexane, chloroform, methanol, acetone and aqueous. The nomenclature, detailed description and photographs of the plant are provided to facilitate its easy identification. The leaf extract showed presence of phenolic compounds, tannin, saponin,...

MRSA from Santa Catarina State, Southern Brazil: intriguing epidemiological differences compared to other Brazilian regions

Palavras-chave: Antibacterial activity; Gymnopetulum chinense; Cucurbits; Streptococcus pyogenes; Antimicrobial resistance.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100438

BJID

Silveira,Alessandro C.O.; Cunha,Gabriela R.; Caierão,Juliana; Cordova,Caio M. de; d'Azevedo,Pedro A..

Methicillin-resistant Staphylococcus aureus(MRSA) is one of the most frequently isolated agents in both nosocomial and community settings. It is a constant challenge for antibacterial therapy. Therefore, it becomes essential to understand the epidemiology of MRSA isolates in the institution and/or region to guide empirical therapy. The objective of this study was to evaluate the epidemiological characteristics of MRSA isolates in the state of Santa Catarina, Brazil, and determine if there is a clonal spread. We evaluated 124 clinical isolates of MRSA obtained from various anatomical sites from patients in the state of Santa Catarina in Southern Brazil. The antimicrobial susceptibility proﬁle was evaluated by disk diffusion and minimum inhibitory...