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Palavra-chave Biotransformation (31) Bioaccumulation (3) Limonene (3) Aroma (2) Genotoxicity (2) Marine flatfish (2) PBDEs (2) Perillyl alcohol (2) 2-Phenylethanol (1) 2.12.00.00-9 prebiotics (1) AIDS/treatment (1) Acetylcholinesterase (1) Acetylcholinesterase inhibitors (1) Allyl Phenyl Carbinol (1) Alpha-pinene (1) Anti-asthmatics (1) Antioxidants (1) Antiretroviral drugs (1) Aroma quality (1) Ascorbic acid (1) Mais... Tipo do documento Info:eu-repo/semantics/article (23) Text (5) Journal article (2) Info:eu-repo/semantics/other (1) Ano 2017 (2) 2016 (1) 2015 (3) 2014 (4) 2012 (2) 2011 (3) 2010 (4) 2009 (1) 2008 (1) 2007 (1) 2006 (1) 2005 (1) 2003 (1) 2002 (2) 2000 (4) Mais... País Brazil (24) France (5) Chile (2) Idioma Inglês (30) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 31 Primeira ... 1 2 ... Última Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production Biological Sciences Deffert, Flávia; Agustini, Bruna Carla; Picheth, Geraldo; Bonfim, Tania Maria Bordin. Fructooligosaccharides are catalyzed by β–fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L-1 sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v-1), and Saccharomyces... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.12.00.00-9 prebiotics; Biotransformation; Identification; PCR. Microbiologia. Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34140 A New Acetylcholinesterase Inhibitor from Green Glycosylation of Trachyloban-19-oic Acid by Mucor plumbeus Anais da ABC (AABC) SANTOS,GABRIEL F. DOS; TAKAHASHI,JACQUELINE A.. ABSTRACT The in vitro metabolism of a widespread natural product, trachyloban-19-oic acid (1), by the fungal species Mucor plumbeus was studied in a sucrose-yeast liquid medium. Two products were isolated, and their structures were determined by spectroscopic means as 7β-hydroxytrachyloban-19-oic acid (5) and trachyloban-19-O-β-D-glucopyranosyl ester (6). To the best of our knowledge, compound 6 is herein reported by the first time in the literature. These compounds were assayed for acetylcholinesterase inhibition along with some related compounds. Compound 6 showed the highest acetylcholinesterase inhibitory activity at 10000 µg/mL among the tested compounds, a result (92.89%) comparable to the activity of the positive control, galanthamine (94.21%).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase inhibitors; Biotransformation; Green glycosylation; Mucor plumbeus; Trachyloban-19-oic acid. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000501961 Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus Anais da ABC (AABC) Ferreira,Nelson R.; Sarquis,Maria Inez M.; Alves,Cláudio N.; Santos,Alberdan S.. The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28"C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l-1 in 120g l-1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Biotransformation; Kojic acid; Secondary metabolite; Fructose-isomerase. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004 Isolation and screening of microorganisms with potential for biotransformation of terpenic substrates BABT Bier,Mário César Jucoski; Poletto,Sabrina; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Medeiros,Adriane Bianchi Pedroni. The objective of the present work was to isolate and select strains with potential to perform the biotransformation of terpenic substrates. Microorganisms obtained from a collection culture and also isolated from a natural source of terpene substrate were tested. Seventeen strains were selected by their resistance to terpenes in potato dextrose agar containing up to 1% of limonene or α-pinene and β-pinene (1:1). Subsequently, 10 strains were selected by their capacity of using these terpenes as sole carbon source in a mineral medium. The biotransformation capacity of these strains was tested and the products obtained were identified by GC-MS. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma; Flavour; Limonene; Β-pinene; Biotransformation. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500020 Biotransformation of Benzaldehyde to L-Phenylacetylcarbinol (L-PAC) by Free Cells of Torulaspora delbrueckii in presence of Beta-Cyclodextrin BABT Shukla,Vilas. B.; Kulkarni,Pushpa R.. Studies were carried out to explore the possibility of decreasing the toxic and inhibitory effects of the substrate benzaldehyde during its biotransformation to L-PAC by free cells of Torulaspora delbrueckii using beta -cyclodextrin (beta -CD). Use of various levels of benzaldehyde and acetaldehyde in presence of 2% of beta -CD showed that, in presence of beta -CD, the organism could tolerate higher levels of benzaldehyde and acetaldehyde. Semi-continuous feeding of benzaldehyde and acetaldehyde was found to increase the yield of L-PAC in comparison with one time feeding. Tipo: Info:eu-repo/semantics/article Palavras-chave: Benzaldehyde; Biotransformation; Cyclodextrin; L-phenylacetycarbinol; Torulaspora delbrueckii. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300003 Enzymatic systems involved in D-limonene biooxidation BABT Menéndez,Pilar; García,Carlos; Rodríguez,Paula; Moyna,Patrick; Heinzen,Horacio. The biooxidation of limonene by an Aspergillus strain resulted in the production of perillyl alcohol and short chain fatty acids. Addition of ketoconazole, a known inhibitor of cytochrome P450 oxydase, eliminated the production of free acids, but did not affect biotransformation to perillyl alcohol. Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Biotransformation; Perillyl alcohol; Cytochrome oxydase; Inhibitors. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200001 Biotransformation of the monoterpene, limonene, by Fusarium verticilloides BABT Oliveira,Brás Heleno de; Strapasson,Rogério Antônio. Limonene, the main constituent of the orange peel oil, was used as substrate for the biotransformation by the fungus Fusarium verticilloides. A hydroxylated derivative of limonene was isolated from the culture broth and characterized by spectroscopic methods. It was identified as perillyl alcohol, an important anti-cancer compound. Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Perillyl alcohol; Biotransformation; Fusarium verticilloides. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100002 Production of allyl phenyl carbinol (APC) by biotrans-formation using Rhizopus arrhizus BABT Shukla,Vilas B.; Kulkarni,Pushpa R.. The objective of the present work was to study the stereoselective synthesis of homo-allylic alcohol using stereoselective hydrolysis by Rhizopus arrhizus and also to study the stereoselective synthesis of allyl phenyl carbinol (APC) or 1-Phenyl-3-butene-1-o by combination of chemical synthesis and biotransformation from cheap raw materials such as benzaldehyde and allyl bromide. Stereospecific synthesis of allyl phenyl carbinol (APC) was achieved by acetate hydrolysis by R. arrhizus giving R (+) enantiomer. Maximum enantiomeric excess of APC was obtained at 16 h where as maximum yield of it was obtained at 48 h of biotransformation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Allyl Phenyl Carbinol; Biotransformation; Rhizopus arrhizus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300001 Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation BABT Guelfi,Marieli; Maioli,Marcos Antonio; Tavares,Marco Aurélio; Mingatto,Fábio Erminio. ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fipronil; Hepatotoxicity; Bioenergetics; Biotransformation; Calcium homeostasis. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600843 Effects of pregnancy and protein-energy malnutrition on monooxygenase O-dealkylation activity in rat liver microsomes BJMBR Kuriyama,S.N.; De-Oliveira,A.C.A.X.; Fidalgo-Neto,A.A.; Paumgartten,F.J.R.. Xenobiotic metabolism is influenced by a variety of physiological and environmental factors including pregnancy and nutritional status of the individual. Pregnancy has generally been reported to cause a depression of hepatic monooxygenase activities. Low-protein diets and protein-energy malnutrition have also been associated with a reduced activity of monooxygenases in nonpregnant animals. We investigated the combined effects of pregnancy and protein-energy malnutrition on liver monooxygenase O-dealkylation activity. On pregnancy day 0 rats were assigned at random to a group fed ad libitum (well-nourished, WN) or to a malnourished group (MN) which received half of the WN food intake (12 g/day). WN and MN rats were killed on days 0 (nonpregnant), 11 or 20... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytochrome P450; Monooxygenases; Xenobiotic metabolism; Undernutrition; Gestation; Biotransformation. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100014 Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites BJM Mercimek,Hatice Aysun; Dincer,Sadik; Guzeldag,Gulcihan; Ozsavli,Aysenur; Matyar,Fatih; Arkut,Afet; Kayis,Fikret; Ozdenefe,Melis Sumengen. Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitroexperiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas sp; Biotransformation; Nitroaromatic explosives; TNT. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100103 Selection of filamentous fungi of the Beauveria genus able to metabolize quercetin like mammalian cells BJM Costa,Eula Maria de M. B.; Pimenta,Fabiana Cristina; Luz,Wolf Christian; Oliveira,Valéria de. Microbial biotransformations constitute an important alternative as models for drug metabolism study in mammalians and have been used for the industrial synthesis of chemicals with pharmaceutical purposes. Several microorganisms with unique biotransformation ability have been found by intensive screening and put in commercial applications. Ten isolates of Beauveria sp genus filamentous fungi, isolated from soil in the central Brazil, and Beauveria bassiana ATCC 7159 were evaluated for their capability of quercetin biotransformation. Biotransformation processes were carried out for 24 up to 96 hours and monitored by mass spectrometry analyses of the culture broth. All strains were able to metabolize quercetin, forming mammalian metabolites. The results were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Beauveria bassiana; Biotransformation; Quercetin. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200036 Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2 BJM Khanolkar,Dnyanada S.; Naik,Milind Mohan; Dubey,Santosh Kumar. A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM). Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed... Tipo: Info:eu-repo/semantics/article Palavras-chave: TBTCl; Morphological alteration; Biotransformation; DBTCl2; Bioremediation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400014 Whole cells in enantioselective reduction of benzyl acetoacetate BJM Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da; Souza,Rodrigo Octavio Mendonça Alves de. The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotransformation; Chiral reduction; Β-ketoester; Kluyveromyces marxianus; Immobilization. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300023 Biotransformation of a cage-like diels-alder adduct and derivatives by Mucor ramosissimus samutsevitsch BJM Ito,Felicia Megumi; Mena,Ana Elisa Maciel; Marques,Maria Rita; Lima,Dênis Pires de; Beatriz,Adilson. The present study aimed to evaluate the ability for biotransformation of the Diels-Alder adduct tricyclo[6.2.1.0(2,7)]undeca-4,9-dien-3,6-dione (1) and two synthetic derivatives by the saprobe fungus Mucor ramosissimus Samutsevitsch. Products from oxidation, isomerization and, regioselective and enantioselective reduction were achieved. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucor ramosissimus; Biotransformation; Diels-Alder adduct. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300019 Biotransformation of poly (epsilon-caprolactone) and poly (vinyl chloride) blend BJM Campos,Adriana de; Franchetti,Sandra Mara Martins; Agnelli,José Augusto Marcondes. Poly (caprolactone) is a famous biodegradable polymer and miscible with PVC, a commercial synthetic polymer, thermal susceptible and non-biodegradable. This blend is important concerning its mechanical properties and biodegradability. In this work, we testified the biomodification of blend films of PVC/PCL by UV-Vis. spectroscopy. The results show us that there is an interaction between the PVC/PCL film and the microrganisms. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotreatment; UV-Vis; PVC/PCL blend; Biotransformation. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500038 Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola BJM Takahashi,Jacqueline Aparecida; Barroso,Henrique de Almeida; Oliveira,Alaíde Braga de. Parameters for a more efficient biotransformation of diterpene-like compounds by the fungus Cephalosporium aphidicola were established by carrying out microscale feedings at several conditions. Experiments were guided by thin layer chromatography and gas chromatography analysis. It was observed that the substrate should be added in ethanol at concentrations between 15 to 30 mg per 100 ml of medium. The extraction of the product showed to be more efficient when carried out from both mycelia and broth and using ethylacetate as the extracting solvent. The experiment should be stopped six days after feeding the substrate to the fungus for the best product yield. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cephalosporium aphidicola; Biotransformation; Diterpene. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200003 Twenty-six years of HIV science: an overview of anti-HIV drugs metabolism BJPS Andrade,Carolina Horta; Freitas,Lenis Medeiros de; Oliveira,Valéria de. From the identification of HIV as the agent causing AIDS, to the development of effective antiretroviral drugs, the scientific achievements in HIV research over the past twenty-six years have been formidable. Currently, there are twenty-five anti-HIV compounds which have been formally approved for clinical use in the treatment of AIDS. These compounds fall into six categories: nucleoside reverse transcriptase inhibitors (NRTIs), nucleotide reverse transcriptase inhibitors (NtRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), cell entry inhibitors or fusion inhibitors (FIs), co-receptor inhibitors (CRIs), and integrase inhibitors (INIs). Metabolism by the host organism is one of the most important determinants of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS/treatment; Drugs/anti-HIV; Drugs/metabolism; Antiretroviral drugs; Biotransformation. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200003 Microbial biotransformation of beclomethasone dipropionate by Aspergillus niger BJPS Ahmad,Saeed; Khaliq,Farhan Hameed; Madni,Asadullah; Shahid,Muhammad Nabeel; Pervaiz,Irfan. In the present research, the steroidal anti-asthmatic drug beclomethasone dipropionate was subjected to microbial biotransformation by Aspergillus niger. Beclomethasone dipropionate was transformed into various metabolites first time from microbial transformation. New drug metabolites produced can act as new potential drug molecules and can replace the old drugs in terms of safety, efficacy, and least resistance. They were purified by preparative thin layer chromatography technique, and their structures were elucidated using modern spectroscopic techniques, such as 13C NMR, 1H NMR, HMQC, HMQC, COSY, and NOESY, and mass spectrometry, such as EI-MS. Four metabolites were purified: (i) beclomethasone 17-monopropionate, (ii) beclomethasone 21-monopropionate,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-asthmatics; Aspergillus niger/use in biotransformation; Beclomethasone dipropionate/microbial biotransformation; Steroids/microbial transformation; Biotransformation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400903 β-carotene biotransformation to obtain aroma compounds Ciênc. Tecnol. Aliment. Uenojo,Mariana; Pastore,Glaucia Maria. Carotenoids are important constituents of food due to their color and because their degradation products generate important volatile compounds in foods. Aroma compounds derived from carotenoids are widely distributed in nature, and they are precursors of many important aromas in foods such as fruits and in flowers as well. They present high aromatic potential and are therefore of great interest to the industries of aromas and fragrances. In this study, more than 300 previously isolated microorganisms with potential for biotransformation of β-carotene present in the culture medium were selected using the plate method; about 80 strains presented capacity to produce aroma compounds and 7 strains were selected by an untrained panel of tasters to generate aroma... Tipo: Info:eu-repo/semantics/article Palavras-chave: Carotenoids; Biotransformation; Β-carotene; Β-ionone; Aroma. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000300039 Registros recuperados: 31 Primeira ... 1 2 ... Última

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