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| Registros recuperados: 44 | |
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| Pedersen,Nina; Tuxen Poulsen,Thomas; Skovgaard Poulsen,Hans. |
| For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requires use of restriction enzymes and ligation reactions. One obstacle is the nucleotide polymorphisms of eukaryotic genomic DNA, which has the consequence that a sequence often differs from published sequences. Therefore sequencing, rigorous restriction enzyme analysis or introduction of suitable sites has to be performed prior to cloning and subcloning. In addition, conventional methods using restriction enzymes, insert purifications and ligations is expensive and labour demanding. We have developed a fast, efficient and inexpensive Cre recombinase-loxP based method, which allows cloning of promoter regions and subcloning of these into a variety of vectors in... |
| Tipo: Journal article |
Palavras-chave: Cloning; Cre recombinase; Plasmid; Reporter genes; Therapeutic genes. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200013 |
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| Dixon,Brian. |
| Aquaculture still faces serious economic impacts due to the loss of animals to disease. A conservative estimate of 5% losses due to disease means that the finfish aquaculture industry loses over $1 billion annually on a global scale. One proven way to prevent costly disease outbreaks is to vaccinate fish against common or known pathogens. Current vaccination schemes still result in losses, however, and this may be due in part to vaccine design. Vaccines are currently designed using state of the art knowledge of immune responses, which is based primarily on mammalian studies. Just how applicable is this information to fish immunity and vaccine design, however? This review discusses what is currently known for teleost fish about two key processes that drive... |
| Tipo: Journal article |
Palavras-chave: Antibody; Antigen presentation; Cloning; Cytokine; Immunity; Major histocompatibility complex receptors. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500014 |
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| Bustami,Yazmin; Yahya,Ahmad Ramli Mohd; Muhammad,Tengku Sifzizul Tengku; Shu-Chien,Alexander Chong; Abdullah,Amirul Al-Ashraf; Noor,Mohd Azizan Mohd; Arip,Yahya Mat. |
| Human erythropoietin (huEPO) is a glycoprotein with important physiological functions, such as erythropoiesis, angiogenesis, and wound healing. A therapeutic protein, huEPO is commonly used to treat patients suffering from renal and non-renal anemia. Recombinant human erythropoietin (rhuEPO) and endogenous huEPO are similar with respect to their biological and chemical properties. In this study, we describe the construction of synthetic huEPO gene to produce rhuEPO. The synthetic huEPO gene was constructed by overlapping oligonucleotides assembly and amplified by polymerase chain reaction (PCR). Twenty oligonucleotide sets, covering the huEPO gene sequence and two newly introduced restriction enzyme sites, were pulled together and amplified using Pfu DNA... |
| Tipo: Journal article |
Palavras-chave: Cloning; Erythropoietin; Oligonucleotide assembly. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300011 |
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| Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando. |
| The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,... |
| Tipo: Journal article |
Palavras-chave: Cloning; Genome; Social representations; Transgenic. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003 |
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| NASCIMENTO FILHO, F. J. do; ATROCH, A. L.; CRAVO, M. da S.. |
| O objetivo e discriminar os clones quanto a produtividade e ao numero de colheitas baseado nos resultados referentes aos ensaios avancados, ou seja, aqueles que antecedem a recomendacao dos materiais, no periodo de 1985 a 1994. Para isso, foram realizadas analises de variancia individuais, para cada experimento, e uma analise de variancia conjuntas, para cada experimento, e uma analise combinada, envolvendo todos os clones, experimentos e anos. |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Melhoramento genetico; Brasil; Amazonas; Manaus; Genetic gain; Cloning; Harvest.; Colheita; Clonagem; Guaraná; Paullinia Cupana.. |
| Ano: 2000 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/670823 |
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| Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V. |
| The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No... |
| Tipo: Text |
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf |
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| Venegas,Felipe; Guillomot,Michel; Vignon,Xavier; Servely,Jean-Luc; Audouard,Chistophe; Montiel,Enrique; Le Bourhis,Daniel; Perón,Sandrine; Soto,Pilar; Rojas,Mariana. |
| The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid... |
| Tipo: Journal article |
Palavras-chave: Pudu deer; Cervidae; Ruminant; Cloning. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026 |
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| Bordon,Karla C F; Wiezel,Gisele A.; Amorim,Fernanda G.; Arantes,Eliane C.. |
| AbstractHyaluronidases are enzymes that mainly degrade hyaluronan, the major glycosaminoglycan of the interstitial matrix. They are involved in several pathological and physiological activities including fertilization, wound healing, embryogenesis, angiogenesis, diffusion of toxins and drugs, metastasis, pneumonia, sepsis, bacteremia, meningitis, inflammation and allergy, among others. Hyaluronidases are widely distributed in nature and the enzymes from mammalian spermatozoa, lysosomes and animal venoms belong to the subclass EC 3.2.1.35. To date, only five three-dimensional structures for arthropod venom hyaluronidases (Apis mellifera and Vespula vulgaris) were determined. Additionally, there are four molecular models for hyaluronidases fromMesobuthus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hyaluronidase; Scorpion; Spider; Caterpillar; Hymenoptera; Insects; Cloning; Heterologous expression; PEGylation; Biotechnological applications. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100214 |
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| Wendling,Ivar; Sturion,José Alfredo; Stuepp,Carlos André; Reis,Cristiane Aparecida Fioravante; Ramalho,Magno Antonio Patto; Resende,Marcos Deon Vilela de. |
| Abstract: The objective of this work was to evaluate the feasibility of early selection of open-pollinated yerba mate (Ilex paraguariensis) progenies, and to classify the best parents and candidates for clones. The germplasm is composed of 140 progenies collected from areas within the natural distribution of the species in Southern Brazil and a commercial genotype (control). The experiment was established in Ivaí, in the state of Paraná, Brazil, in March 1997, in randomized complete blocks, with ten replicates, and linear plots of six plants. The commercial mass (kg per plant) of leaves and branches smaller than 7 mm was harvested at 2.5, 4.5, 6.5, and 18.7 years of age. The statistical evaluation was performed using the mixed model procedure with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ilex paraguariensis; Cloning; Genetic correlation; Progeny x harvesting-age interaction. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300279 |
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| Registros recuperados: 44 | |
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