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Registros recuperados: 37 | |
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Souza,Valéria Nogueira de; Martins,Maurício Laterça; Moraes,Flávio Ruas de; Kronka,Sérgio do Nascimento. |
Tropical fishes like Leporinus macrocephalus Garavello & Britski, 1988 ("piauçu") and the hybrid "tambacu" (Piaractus mesopotamicus Holmberg, 1887 "pacu" male x Colossoma macropomum Cuvier, 1818 "tambaqui" female) were experimentally infected with four inocula of trophonts of Ichthyophthirius multifiliis Fouquet, 1876 (Protozoa, Ciliophora) and the infection level was evaluated. Each one of twelve experimental units consisted of a tank with 200 liters of water and two specimens of each kind of fish. The following treatments were designed to each tank, in triplicate: 8, 16,32 and 64 trophonts. After 8 days of exposure the fishes were killed and the number of trophonts was counted at a stereoscopic microscope. This assay showed to be feasible at... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ichthyophthirius multifiliis; Tropical fish; Experimental infection; Susceptibility. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000300016 |
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PINHEIRO, R. R; SANTA ROSA, J.. |
Na Embrapa.CNPC foi realizado um trabalho com o objetivo de identificar algumas plantas toxicas para a especie caprina. Neste experimento foram testados a Salsa (Ipomoea asarifolia), Canudo (Ipomoea fistulosa), Mata zombando (Schultesia sp.), Pau moco (Luetzelburgia auriculata), Angico preto (Piptadenia macrocarpa), Tinqui (Mascagnia rigida) e Timbauba (Enterolobium contortisilignuum). Destas, as comprovadamente toxicas foram a salsa, o pau moco, a timbauba e o canudo. De acordo com varios autores a ingestao da Ipomoea asarifolia causa intoxicacao com sintomas de ordem nervosa em bovinos, ovinos e caprinos, o que conduz a perdas economicas decorrentes da morte de caprinos jovens principalmente nas epocas de escassez de alimentos. Isto ocorre em virtude da... |
Tipo: Parte de livro |
Palavras-chave: Ipomoea asarifolia; Intoxication.; Experimental infection; Caprino; Intoxicação; Planta tóxica; Salsa; Goats; Poisonous plants; Toxicity.. |
Ano: 1996 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/514752 |
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SCHWARZ, D. G. G.; PIETRALONGA, P. A. G.; SOUZA, M. C. C.; CARVALHO, I. A.; CRUZEIRO, R. S.; MALAQUIAS, J. V.; BENJAMIM, L. A.; SILVA JÚNIOR, A.; MOREIRA, M. A. S.. |
Resumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e... |
Tipo: Separatas |
Palavras-chave: Johne’s disease; Experimental infection; Doença de Johne; Murinos; Infecção experimental.; Mycobacterium avium; Mice.. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036032 |
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GALIZA, Y. S. de; ELOY, A. M. X.; PINHEIRO, R. R.; PEIXOTO, R. M.; LIMA, A. M. C.; BARROSO, M. L. da S.; FONSECA, L. M.. |
Abstract: Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host?s immune system, they are not sufficient to be detected by serological tests. Acute infections begin with an incubation period, during which the viral genome replicates and host innate responses are initiated. Matrix metalloproteinases (MMPs) are enzymes that play an important role in the physiological and pathological processes of tissue remodeling. The present study aimed to evaluate the expression of MMPs and their activity in the blood serum of male goats experimentally infected with caprine arthritis encephalitis virus (CAEV). Five dairy male... |
Tipo: Artigo de periódico |
Palavras-chave: MMPs; Zimografia; Encefalite artrite caprina; Infecção experimental; CAEV; Zymography; Caprine arthritis encephalit virus; Experimental infection; Caprino; Doença Animal; Protease; Diagnostico; Virose Animal; Small ruminants; Goats; Goat diseases; Infectious diseases; Lentivirus; Caprine arthritis-encephalitis; Caprine arthritis encephalitis virus; Disease diagnosis; Semiarid zones; Brazil. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127176 |
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PINHEIRO, R. R.; SANTA ROSA, J.. |
O objetivo do trabalho foi de avaliar as alteracoes no hemograma, na urinalise e nos niveis sericos de proteina total, albumina, glicose, ureia, creatinina, aspartato aminotransferase (AST), bilirrubina e fosfatase alcalina em caprinos intoxicadosm experimentalmente, com a Ipomoea asarifolia. |
Tipo: Parte de livro |
Palavras-chave: Urinálise; Bioqímica sérica; Hemogram; Serum biochemistry; Experimental infection; Intoxication.; Caprino; Intoxicação; Planta tóxica; Salsa; Hemograma; Goats; Poisonous plants; Toxicity; Urinalysis.. |
Ano: 1996 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/514753 |
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Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC. |
This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%... |
Tipo: Resistencia a infecção |
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology. |
Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471 |
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Cadioli,F.A.; Marques,L.C.; Machado,R.Z.; Alessi,A.C.; Aquino,L.P.C.T.; Barnabé,P A.. |
Five adult donkeys were experimentally infected with Brazilian strain of Trypanosoma evansi originally isolated from a naturally infected dog to study the hematological biochemical and histopathological alterations during the evolution of the disease. The course of the experimental infection was followed up to 145 days. Hematological analyses of the infected donkeys revealed a marked decline in hemoglobin, packed-cell volume, and erythrocyte count. Anemia was observed after successive peaks of parasitemia. Biochemical analyses showed increased levels of icterus index, serum globulins and decreased serum albumin and glucose values. All infected donkeys revealed enlargement of spleen and its white pulp, enlargement of mediastinal lymph nodes and lungs... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Donkey; Experimental infection; Trypanosoma evansi; Trypanosomiasis. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500008 |
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Falkowski,G.J.S.; Sandri,P.F.; Tiyo,R.; Aleixo,D.L.; Araújo,S.M.. |
The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain - Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7th-11th and 15th-18th days of infection. There were significant differences (P<0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P>0.05) between the groups in food consumption, exploration of self-cleaning and skin... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clinical analysis; Chagas disease; Experimental infection; Mice; Trypanosoma cruzi. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600020 |
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Martins,M.L.; Ghiraldelli,L.; Garcia,F.; Onaka,E.M.; Fujimoto,R.Y.. |
This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental infection; Nematoda; Camallanus; Crustacea; Notodiaptomus. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200018 |
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Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W.. |
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001 |
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Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies. |
Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015 |
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Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.. |
The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004 |
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Chaves,Guilherme M.; Cavalcanti,Maria Auxiliadora de Q.; Carneiro-Leão,Ana Maria dos A.; Lopes,Silvia L.. |
With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL-1) activity. The other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase (Pz=0.482) and proteinase (0.780 U.mL-1) activity. The strains were inoculated via intraperitoneum in immunosuppressed and non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and kidneys were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Extracellular enzymes; Virulence; Experimental infection. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300010 |
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Registros recuperados: 37 | |
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