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POTION: an end-to-end pipeline for positive Darwinian selection detection in genome-scale data through phylogenetic comparison of protein-coding genes. Repositório Alice
HONGO, J. A.; CASTRO, G. M. de; CINTRA, L. C.; ZERLOTINI, A.; LOBO, F. P..
Abstract: Background: Detection of genes evolving under positive Darwinian evolution in genome-scale data is nowadays a prevailing strategy in comparative genomics studies to identify genes potentially involved in adaptation processes. Despite the large number of studies aiming to detect and contextualize such gene sets, there is virtually no software available to perform this task in a general, automatic, large-scale and reliable manner. This certainly occurs due to the computational challenges involved in this task, such as the appropriate modeling of data under analysis, the computation time to perform several of the required steps when dealing with genome-scale data and the highly error-prone nature of the sequence and alignment data structures needed...
Tipo: Artigo de periódico Palavras-chave: Potion; Evolução Darwiniana; Comparative genomics; Positive selection; Gene; Fenótipo; Pesquisa; Genomics; Phylogeny; Genes; Genome.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1027912
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Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Repositório Alice
SANTOS, F. C.; VALLE, C. B. do; CHIARI, L..
Tipo: Artigo de periódico Palavras-chave: Genome organization; Transposable elements; Repetitive DNA.; Metaviridae; Centromeres; Fish; Genetics; Genomics; Grasses; Chromosomes; In situ hybridization; Retrotransposons; Transposons..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1040145
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Genomic selection for genotype performance and environmental stability in Coffea canéfora. Repositório Alice
ADUNOLA, P.; FERRÃO, M. A. G.; FERRÃO, R. G.; FONSECA, A. F. A. da; VOLPI, P. S.; COMÉRIO, M.; VERDIN FILHO, A. C.; MUNOZ, P. R.; FERRÃO, L. F. V..
Coffee is one of the most important beverages and trade products in the world. Among the multiple research initiatives focused on coffee sustainability, plant breeding provides the best means to increase phenotypic performance and release cultivars that could meet market demands. Since coffee is well adapted to a diversity of tropical environments, an important question for those confronting the problem of evaluating phenotypic performance is the relevance of genotype-by-environment interaction. As a perennial crop with a long juvenile phase, coffee is subjected to significant temporal and spatial variations. Such facts not only hinder the selection of promising materials but also cause a majority of complaints among growers. In this study, we hypothesized...
Tipo: Artigo de periódico Palavras-chave: Coffea Canephora; Genomics; Genotype; Plant breeding; Prediction.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159395
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Draft genome sequencing of the Yeast Spathaspora arborariae sp. Repositório Alice
SANTOS, E. de M.; FRANCO, G. R.; STAMBUK, B. J. C. U.; ROSA, C. A.; LOBO, F. P..
This work provides the description of a draft genome of S. arborariae.
Tipo: Anais e Proceedings de eventos Palavras-chave: Sequência de genoma; Yeast Spathaspora arborariae sp.; Sequence analysis; Yeasts; Genomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946642
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GO enrichment of microarray data from maize root under drough. Repositório Alice
NODA, R. W.; LAAT, D. M. de; GUIMARÃES, C. T.; SOUZA, T. C. de; DIAS, L. L. C.; LOBO, F. P.; MAGALHÃES, J. V. de; COIMBRA, R. S.; MAGALHÃES, P. C.; ZERLOTINI, A.; FRANCO, G. M.; CARNEIRO, N. P..
In this study microarray data from roots of a drought tolerant inbred maize line (Embrapa?s breeding program) under two water regimes was analyzed. The analysis revealed that 746 genes were differentially expressed, of which 455 were up- and 291 were down-regulated.
Tipo: Parte de livro Palavras-chave: Genômica; Milho; Microarray technology; Corn; Genomics.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981622
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Introduction to genome editing in plants. Repositório Alice
LOPES FILHO, J. H.; SILVA, V. C. H. da; SANTOS, J. C. dos; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R..
Present and future challenges of modern agriculture. Gene editing tools for precision crop breeding. Genome editing methodologies. Origin of the CRISPR/Cas technology. Overview of mechanisms and enzymes. Application of CRISPR in agriculture. Productivity increase. Quality improvement. Resistance to biotic factors. Resistance to abiotic factors. Accelerated and de novo domestication of wild species. Perspectives and conclusions.
Tipo: Parte de livro Palavras-chave: Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein; Repetições palindrômicas agrupadas e regularmente espaçadas; Edição genômica; Genômica; Sistema CRISPR/Cas; Técnicas de edição de genomas; Transcription Activator-Like Effector Nuclease; Zinc Finger Nucleases; Agricultura; Biologia Molecular; Melhoramento Genético Vegetal; Agriculture; Genetic improvement; Genomics.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131965
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Genome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer). Repositório Alice
LOBO, F. P.; CINTRA, L. C.; VARELA, E. S.; ALVES, A. L.; SILVA, N. M. A. da; IANELLA, P.; PAIVA, S. R.; CAETANO, A. R..
The South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were...
Tipo: Anais e Proceedings de eventos Palavras-chave: Bioinformática; Genômica; Sequenciamento de genoma; Bioinformatics; Genomics; Genome assembly; Sequence analysis.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1038884
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Genome-wide association study of cassava starch paste properties. Repositório Alice
SANTOS, C. S. DOS; SOUSA, M. B.; BRITO, A. C.; OLIVEIRA, L. A. de; CARVALHO, C. W. P. de; OLIVEIRA, E. J. de.
An understanding of cassava starch paste properties (CSPP) can contribute to the selection of clones with differentiated starches. This study aimed to identify genomic regions associated with CSPP using different genome-wide association study (GWAS) methods (MLM, MLMM, and Farm-CPU). The GWAS was performed using 23,078 single-nucleotide polymorphisms (SNPs). The rapid viscoanalyzer (RVA) parameters were pasting temperature (PastTemp), peak viscosity (PeakVisc), hot-paste viscosity (Hot-PVisc), cool-paste viscosty (Cold-PVisc), final viscosity (FinalVis), breakdown (BreDow), and setback (Setback). Broad phenotypic and molecular diversity was identified based on the genomic kinship matrix. The broad-sense heritability estimates (h2) ranged from moderate to...
Tipo: Artigo de periódico Palavras-chave: Fécula; Mandioca; Genoma; Cassava starch; Food paste; Genomics.
Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150198
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KOMODO2 - Large-scale genomic inference. Repositório Alice
LOBO, F.; HONGO, J..
ISCB-Latin America 2014. Pôster D16.
Tipo: Anais e Proceedings de eventos Palavras-chave: Genômica; Genomics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008419
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A comparison of regression methods based on dimensional reduction for genomic prediction. Repositório Alice
COSTA, J. A. da; AZEVEDO, C. F.; NASCIMENTO, M.; SILVA, F. F. e; RESENDE, M. D. V. de; NASCIMENTO, A. C. C..
multicollinearity and high dimensionality problems, making it impossible to obtain stable estimates through the traditional method of estimation based on ordinary least squares. To overcome such challenges, dimensionality reduction methods have been proposed, because of their simple theory and easy application. We compared three dimensionality reduction methods: Principal Components Regression (PCR), Partial Least Squares (PLS), and Independent Components Regression (ICR). An important step for dimensionality reduction and prediction is selecting the number of components, as it affects the linear combinations of the explanatory variables. The linear combinations are inserted into the model to predict the response based on a reduced number of parameters. We...
Tipo: Artigo de periódico Palavras-chave: Regression analysis; Genomics.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139234
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In silico identification of coffee genome expressed sequences potentially associated with resistance to diseases. Repositório Alice
ALVARENGA, S. M.; CAIXETA, E. T.; HUFNAGEL, B.; THIEBAUT, F.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; SAKIYAMA, N. S..
Sequences potentially associated with coffee resistance to diseases were identified by in silico analyses using the database of the Brazilian Coffee Genome Project (BCGP). Keywords corresponding to plant resistance mechanisms to pathogens identified in the literature were used as baits for data mining. Expressed sequence tags (ESTs) related to each of these keywords were identified with tools available in the BCGP bioinformatics platform. A total of 11,300 ESTs were mined. These ESTs were clustered and formed 979 EST-contigs with similarities to chitinases, kinases, cytochrome P450 and nucleotide binding site-leucine rich repeat (NBS-LRR) proteins, as well as with proteins related to disease resistance, pathogenesis, hypersensitivity response (HR) and...
Tipo: Artigo de periódico Palavras-chave: Data mining; ESTs.; Coffea; Genomics; Bioinformatics..
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880494
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Selection signatures in Canchim beef cattle Repositório Alice
URBINATI, I.; STAFUZZA, N. B.; OLIVEIRA, M. T.; CHUD, T. C. S.; HIGA, R. H.; REGITANO, L. C. de A.; ALENCAR, M. M. de; BUZANSKAS, M. E.; MUNARI, D. P..
Background: Recent technological advances in genomics have allowed the genotyping of cattle through single nucleotide polymorphism (SNP) panels. High-density SNP panels possess greater genome coverage and are useful for the identification of conserved regions of the genome due to selection, known as selection signatures (SS). The SS are detectable by different methods, such as the extended haplotype homozygosity (EHH); and the integrated haplotype score (iHS), which is derived from the EHH. The aim of this study was to identify SS regions in Canchim cattle (composite breed), genotyped with high-density SNP panel. Results: A total of 687,655 SNP markers and 396 samples remained for SS analysis after the genotype quality control. The iHS statistic for each...
Tipo: Artigo de periódico Palavras-chave: Genômica; Polimorfismo de nucleotídeo único; Extended haplotype homozygosity; Gado de corte; Composite breeds; Genomics; Single nucleotide polymorphism; Beef cattle; Quantitative trait loci.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061492
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New single sucleotide polymorphisms in Guzerá breed revealed by whole-genome re-sequencing. Repositório Alice
CRUZ, I. R.; GERALDO, J. A.; OLIVEIRA, F. S. de; LEITE, L. R.; ARAÚJO, F.; ZERLOTINI, A.; LOPES, B. C.; ARBEX, W. A.; MACHADO, M. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; MARTINS, M. F.; SILVA, M. V. G. B.; COIMBAR, R. S.; CARVALHO, M. R. S.; OLIVEIRA, G..
The objective of our work was to sequence and assemble the Guzerá genome in order to identify race-­specific variations that might be used in breeding programs.
Tipo: Anais e Proceedings de eventos Palavras-chave: Polimorfismo de nucleotídeo único; Criação de animais; Análise de sequência de genomas; Single nucleotide polymorphism; Animal breeding; Sequence analysis; Genomics.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/973157
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POTION: a massive parallel program for identification of homologous genes under positive selection on genomic scale datasets. Repositório Alice
HONGO, J.; CASTRO, G.; SILVA, F.; CINTRA, L.; ZERLOTINI, A.; LOBO, F..
The search for positive selection is also highly parallelizable, since each group of homologous can be analyzed independently. Here we describe POTION (POsitive selecTION), a massively parallel software to automatically detect positive selection on genomic scale data, ideal for analysis of large datasets in cluster infrastructures
Tipo: Anais e Proceedings de eventos Palavras-chave: Bioinformática; Genômica; Bioinformatics; Computer software; Genomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946632
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Genômica e biotecnologia aplicadas a adaptação a mudanças climáticas. Repositório Alice
GERHARDT, I. R.; DANTE, R. A..
Mudanças climáticas globais e seus impactos na agricultura. Pesquisa e desenvolvimento de tecnologias transgênicas. Genômica funcional para descoberta de genes de valor agronômico. Bancos de dados genômicos. Genômica comparativa. Análise filogenética de famílias gênicas. Aplicações de tecnologias de sequenciamento de próxima geração. Seleção em larga escala de eventos transgênicos. A Unidade Mista de Pesquisa em Genômica Aplicada a Mudanças Climáticas (UMiP GenClima).
Tipo: Parte de livro Palavras-chave: Mudanças climáticas; Genômica; Biotecnologia; Climate change; Genomics; Biotechnology.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010699
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Elaeis oleifera genome draft - genomics of american oil palm. Repositório Alice
LIMA, R. S.; LOBO, F. P.; CARAZZOLLE, M. F.; COSTA, G. G. L.; RODRIGUES, E. L. de C.; ALVES, A. A.; YAMAGISHI, M. E. B.; SOUZA JUNIOR, M. T.; FORMIGHIERI, E. F..
In that scenario, Embrapa Agroenergy is making efforts to improve bioenergy generation and accelerate selection of superior genotypes, through NGS and large-scale genotyping technologies. Some colleagues have already estimated of Elaeis species genome size using flow cytometry, and in parallel, we sequenced one lane of Illumina HiSeq 2000 for three E. oleifera and one E. guineensis accessions, for initial comparison.
Tipo: Anais e Proceedings de eventos Palavras-chave: Genoma de palmeira; Óleo de palma.; Elaeis Guineensis.; Palm oils; Genomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946637
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Selection signatures in Canchim beef cattle. Repositório Alice
URBINATI, I.; STAFUZZA, N. B.; OLIVEIRA, M. T.; CHUD, T. C. S.; HIGA, R. H.; REGITANO, L. C. de A.; ALENCAR, M. M. de; BUZANSKAS, M. E.; MUNARI, D. P..
Tipo: Artigo de periódico Palavras-chave: SNP; Cruzamento; Cruzamento Animal; Genótipo; Genomics.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1047351
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On the accuracy of threshold genomic prediction models for leaf miner and leaf rust resistance in arabica coffee. Repositório Alice
CARVALHO, H. F.; FERRÃO, L. F. V.; GALLI, G.; NONATO, J. V. A.; PADILHA, L.; MALUF, M. P.; RESENDE JR., M. F. R. de; FRITSCHE-NETO, R.; GUERREIRO-FILHO, O..
Obtaining resistance cultivars for leaf miner and leaf rust are the main important strategy of Brazil?s national coffee breeding program. The narrow genetic basis, and founder effect consequences, lead to challenges in quantifying and detecting genetic diversity for these traits. Biotechnology tools allied with classical breeding strategies are powerful in detecting variability and deploying a precision selection. The selection based on the genetic merit of an individual obtained from thousands of single nucleotide polymorphism effects is known as genomic selection. The ordinal scale principally makes the resistance evaluation of the leaf rust and leaf miner of the score, categorizing the phenotypes following the discrete (ordinal) distribution. Hence,...
Tipo: Artigo de periódico Palavras-chave: Coffea arabica var. arabica; Leucoptera; Hemileia; Leaf rust; Genomics.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156023
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Genomic analysis of soybean accessions for allelic variations identification in brazilian germplasm. Repositório Alice
SANTOS, J. V. M. dos; JOSHI, T.; KHAN, S. M.; LIU, Y.; WANG, J.; VUONG, T. D.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, M. F. de; VALLIYODAN, B.; XU, D.; NGUYEN, H. T.; ABDELNOOR, R. V..
Tipo: Parte de livro Palavras-chave: Soja; Genoma; Melhoramento genético vegetal; Soybeans; Genomics.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1018778
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Using genomic signature to detect cases of coevolution in viral/host systems - the Poxviridae family. Repositório Alice
LOBO, F.; COUTINHO, T..
ISCB-Latin America 2014. Pôster D15.
Tipo: Anais e Proceedings de eventos Palavras-chave: Genômica; Poxviridae; Genomics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008417
Registros recuperados: 97
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