Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 112
Primeira ... 123456 ... Última
Imagem não selecionada

Imprime registro no formato completo
Quantitative determination by HPLC of iridoids in the bark and latex of himatanthus sucuuba Acta Amazonica
Silva,Jefferson Rocha de Andrade; Amaral,Ana Claudia Fernandes; Silveira,Cleoni Virgínio da; Rezende,Claudia Moraes; Pinto,Angelo Cunha.
The main iridoids from the bark and latex of Himatanthus sucuuba were isolated and characterised by spectroscopic methods. HPLC was used for the quantitative analyses of these iridoids and the chromatograms of bark and latex showed a similar iridoid composition. Both parts of the plant are used in folk medicine for the treatment of various ailments.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apocynaceae; HPLC; Himatanthus sucuuba; Iridoids; Quantitative determination.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000100014
Imagem não selecionada

Imprime registro no formato completo
ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES Acta biol.Colomb.
OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda.
In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017
Imagem não selecionada

Imprime registro no formato completo
Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate Agronomy
Guedes, Mayara Neves Santos; Pio, Rafael; Maro, Luana Aparecida Castilho; Lage, Fabíola Fonseca; Abreu, Celeste Maria Patto de; Saczk, Adelir Aparecida.
Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 total phenols; Free radicals; HPLC; Rubus spp. Fitotecnia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/28413
Imagem não selecionada

Imprime registro no formato completo
Gestational diabetes: a risk of puerperal hypovitaminosis A? Anais da ABC (AABC)
RESENDE,FERNANDA B.S.; LIRA,LARISSA Q. DE; GRILO,EVELLYN C.; LIMA,MAYARA S.R.; DIMENSTEIN,ROBERTO.
The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colostrum; Gestational diabetes; HPLC; Hypovitaminosis A; Postpartum.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100463
Imagem não selecionada

Imprime registro no formato completo
Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach. Anais da ABC (AABC)
EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN.
Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621
Imagem não selecionada

Imprime registro no formato completo
Quantification of anti-nutritional factors and their correlations with protein and oil in soybeans Anais da ABC (AABC)
BUENO,RAFAEL D.; BORGES,LEANDRO L.; GOD,PEDRO I.V. GOOD; PIOVESAN,NEWTON D.; TEIXEIRA,ARLINDO I.; CRUZ,COSME DAMIÃO; BARROS,EVERALDO G. DE.
ABSTRACT Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Carbohydrates; Anti-nutritional factors; Quantitative analysis; HPLC; NIR spectroscopy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100205
Imagem não selecionada

Imprime registro no formato completo
Acaricidal activity of leaves of Morus nigra against the cattle tick Rhipicephalus microplus Arq. Bras. Med. Vet. Zootec.
Dantas,A.C.S.; Freire,D.P.; Souza,G.R.; Almeida,J.R.G.S.; Rolim,L.A.; Castro,R.N.; Horta,M.C..
ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tick control; Acaricide; Plant extract; Mulberry; HPLC.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300523
Imagem não selecionada

Imprime registro no formato completo
Caseinomacropeptide index in UHT whole milk stored under different conditions of temperature and time Arq. Bras. Med. Vet. Zootec.
Villanoeva,C.N.B.C.; Andrade,E.H.P.; Baffa Junior,J.C.; Souza,M.R.; Cerqueira,M.M.O.P.; Fonseca,L.M.; Penna,C.F.A.M.; Leite,M.O..
Caseinomacropeptide (CMP) index is a method used to detect adulteration of milk by addition of cheese whey, since CMP is a glycopeptide characteristic produced during cheesemaking, and soluble in the whey phase. The objective of this work was to evaluate the caseinomacropeptide index of UHT milk stored under different temperatures. Six batches of recently processed UHT milk were collected and stored under three temperatures (21ºC, 6ºC, and -12ºC) and analyzed by HPLC in the day of the milk collection (day 0) and at 30, 60, 90, and 120 days of storage. The experiment was run as a randomized block design with a 3x5 factorial arrangement, and the Student-Newman-Keuls (SNK) method was used as the post-hoc test (p = 0.05). There was a progressive increase of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: UHT whole milk; Caseinomacropeptide; HPLC.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100039
Imagem não selecionada

Imprime registro no formato completo
Liberação intraocular de ofloxacina associada a lente de contato biossintética em ceratite bacteriana experimental em cães Arq. Bras. Med. Vet. Zootec.
Cremonini,D.N.; Ranzani,J.J.T.; Cunha,M.L.R.S.; Tonin,F.G.; Brandão,C.V.S.; Messadeq,Y..
Avaliou-se a concentração de ofloxacina liberada por uma lente de contato de membrana de celulose biossintética, para tratamento de ceratite bacteriana experimental em cães, pela inoculação de Staphylococcus aureus intraestromal. Comparou-se o tratamento com a lente de contato biossintética impregnada com ofloxacina à terapia tópica convencional. Realizou-se avaliação microbiológica e dosagem de ofloxacina no humor aquoso por meio do método de cromatografia líquida de alto rendimento (HPLC). Houve diferença estatística na contagem de colônias bacterianas entre os olhos com ceratite e os demais grupos, no primeiro dia de coleta. O biomaterial, impregnado com ofloxacina, promoveu liberação gradual durante o período de avaliação, aos três e sete dias; no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cão; Ceratite bacteriana; Membrana biossintética; Ofloxacina; HPLC.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400004
Imagem não selecionada

Imprime registro no formato completo
HPLC identification of isoniazid residues in bovine milk Arq. Bras. Med. Vet. Zootec.
Leite,R.M.H.; Leite,R.C.; Lima,J.A.; Fóscolo,C.B.; Mota,P.M.P.C.; Lobato,F.C.F.; Lage,A.P..
The high pressure liquid chromatography (HPLC) was used for the identification of isoniazid (isonicotinic acid hydrazide) in the milk of cattle treated with a dose of 25 mg/kg/day in alternated days. The effect of milk pasteurization on the isoniazid residue concentration was also studied. The drug excretion presented a cyclic variation, with higher levels in the first day after administration (aa), a mean of 1104.48µg/l, and a decrease two days aa, with a mean of 104.12µg/l. Four days after the last administration of the drug it was not possible to identify residues of isoniazid in the milk of treated animals. Body weight and milk yield influenced the amount of the excreted drug, and pasteurization decreased (mean 47.07%) the concentration of isoniazid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Tuberculosis; Isoniazid; Milk; HPLC.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600018
Imagem não selecionada

Imprime registro no formato completo
Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography Arq. Bras. Med. Vet. Zootec.
Lasmar,M.M.; Leite,M.O.; Fonseca,L.M.; Souza,M.R.; Cerqueira,M.M.O.P.; Penna,C.F.A.M.; Couto,C.N.B.; Ferreira,J.M..
High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Raw milk; Bronopol; Cheese whey detection; HPLC.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600037
Imagem não selecionada

Imprime registro no formato completo
Detection of cheese whey and caseinomacropeptide in fermented milk beverages using high performance liquid chromatography Arq. Bras. Med. Vet. Zootec.
Andrade,E.H.P.; Souza,M.R.; Fonseca,L.M.; Penna,C.F.A.M.; Cerqueira,M.M.O.P.; Roza,T.; Seridan,B.; Resende,M.F.S.; Pinto,F.A.; Villanoeva,C.N.B.C.; Leite,M.O..
Cheese whey level and caseinomacropeptide (CMP) index of fermented milk beverages added with four levels of cheese whey (0, 10, 20, and 40%) and stored at 8-10oC for 0, 7, 14 and 21 days were determined by high performance liquid chromatography-gel filtration (HPLC-GF). Additionally, the interference of the starter culture and the storage time on the detection of cheese whey and CMP were investigated. Refrigerated storage up to 21 days did not affect (P>0.05) cheese whey and CMP amounts in milk (0% of cheese whey) and in fermented milk beverages added with 10 and 20% of cheese whey (P>0.05). However, cheese whey and CMP amounts were higher than expected (P<0.05) in fermented milk beverages added with 40% of cheese whey and stored for 21 days.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented milk beverages; Cheese whey; CMP; HPLC.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300959
Imagem não selecionada

Imprime registro no formato completo
Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments Biocell
Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan.
Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Salicylic acid; UV-B.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006
Imagem não selecionada

Imprime registro no formato completo
In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica Biol. Res.
Moukette,Bruno Moukette; Pieme,Constant Anatole; Njimou,Jacques Romain; Biapa,Cabral Prosper Nya; Marco,Bravi; Ngogang,Jeanne Yonkeu.
BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose...
Tipo: Journal article Palavras-chave: Antioxidant; Radical scavenging; HPLC; Monodora myristica; Non-timber forest product.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100015
Imagem não selecionada

Imprime registro no formato completo
Growth, delayed fluorescence and pigment composition of four Prorocentrum minimum strains growing at two salinities Biol. Res.
BERDEN-ZRIMEC,MAJA; FLANDER-PUTRLE,VESNA; DRINOVEC,LUKA; ZRIMEC,ALEXIS; MONTI,MARINA.
Prorocentrum minimum is a potentially harmful and widely distributed marine dinoflagellate. Several P. minimum strains have already been studied, showing phylogenetical relations of strains isolated from the same geographical regions. Similarity among the strains was further examined on the basis of their physiology. Pigment composition and concentration, as well as delayed fluorescence (DF) decay kinetics and intensity, were measured in four P. minimum strains isolated from the Baltic and Adriatic Seas. The strains were grown at two salinities characteristic of the Baltic (8 PSU) and North Adriatic Seas (32 PSU). Strain differences in DF decay kinetics and growth did not always follow their genetic relations. While two strains showed similarities to the...
Tipo: Journal article Palavras-chave: Adriatic Sea; Baltic Sea; Delayed fluorescence; HPLC; Prorocentrum minimum; Strains.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100003
Imagem não selecionada

Imprime registro no formato completo
Altered dopamine levels induced by the parasite Profilicollis antarcticus on its intermediate host, the crab Hemigrapsus crenulatus Biol. Res.
ROJAS,JOSÉ MIGUEL; OJEDA,F. PATRICIO.
A serotonergic pathway is apparently involved in parasite-host interactions. Previous studies conducted in our laboratory showed increased rates in oxygen consumption and alterations in body posture in the crab Hemigrapsus crenulatus parasitized by the acanthocephalan, Profilicollis antarcticus. Such changes may be related to the functions described for biogenic amines in crustaceans. During the infective stage the acanthocephalans live freely in the hemocelomic cavity, suggesting that the possible alteration induced by biogenic amines may be related to their neurohormonal function in crustaceans. To test whether the presence of P. antarcticus produced neurohormonal changes in its intermediate host, H. crenulatus, we analyzed serotonin and dopamine levels...
Tipo: Journal article Palavras-chave: Acanthocephalans; Biogenic amines; HPLC; Parasite-host interaction; Parasite transmission.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200015
Imagem não selecionada

Imprime registro no formato completo
Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey Biol. Res.
Eyduran,Sadiye Peral; Akin,Meleksen; Ercisli,Sezai; Eyduran,Ecevit; Maghradze,David.
BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz....
Tipo: Journal article Palavras-chave: Table grape; Biochemical composition; HPLC; Spectrophotometer; Germplasm characterization.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100002
Imagem não selecionada

Imprime registro no formato completo
Phenolic compounds and vitamins in wild and cultivated apricot (Prunus armeniaca L.) fruits grown in irrigated and dry farming conditions Biol. Res.
Kan,Tuncay; Gundogdu,Muttalip; Ercisli,Sezai; Muradoglu,Ferhad; Celik,Ferit; Gecer,Mustafa Kenan; Kodad,Ossama; Zia-Ul-Haq,Muhammad.
BACKGROUND: Turkey is the main apricot producer in the world and apricots have been produced under both dry and irrigated conditions in the country. In this study, phenolic compounds and vitamins in fruits of one wild (Zerdali) and three main apricot cultivars ('Cataloglu', 'Hacihaliloglu' and 'Kabaasi') grown in both dry and irrigated conditions in Malatya provinces in Turkey were investigated. RESULTS: The findings indicated that higher content of phenolic compounds and vitamins was found in apricot fruits grown in irrigated conditions. Among the cultivars, 'Cataloglu' had the highest rutin contents both in irrigated and dry farming conditions as 2855 µg in irrigated and 6952 µg per 100 g dried weight base in dry conditions and the highest chlorogenic...
Tipo: Journal article Palavras-chave: Apricot; Phytochemicals; Ripening; Irrigation; HPLC.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100046
Imagem não selecionada

Imprime registro no formato completo
A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee BABT
Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
Imagem não selecionada

Imprime registro no formato completo
Production of specific polyclonal antibodies anti-human factor IX BABT
Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010
Registros recuperados: 112
Primeira ... 123456 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional