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Registros recuperados: 34 | |
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Castro,Juliana P.; Moraes,Ana Paula; Chase,Mark W.; Santos,Angeline M. S.; Batista,Fabiane R. C.; Felix,Leonardo P.. |
ABSTRACT Cactaceae species are karyotypically well-known with x = 11 and chromosome number variation due mainly to polyploidization. However, both assumptions are based on descriptive observations without taking an evolutionary framework of Cactaceae into account. Aiming to confirm these hypotheses in an evolutionary context, we obtained chromosome numbers for 20 species of Cactoideae, performed an extensive review of chromosome number for the family, and analyzed these data using a phylogenetic approach. The karyotypes presented here were characterized by CMA/DAPI banding, and for six species 5S and 45S rDNA sites were located. Our data, along with a survey of the literature, reinforce the long-standing hypothesis of a x = 11 as the base chromosome number... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Base chromosome number; CMA/DAPI; Dysploidy; Heterochromatin; Polyploidy; RDNA. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100135 |
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Chiarini,Franco E; Gauthier,Martha J. |
The chromosomes of Solanum nelsonii, S. sandwicense (endemic to the Hawaii islands) and S. vespertilio (from the Canary islands) were studied by means of classical staining, CMA/DAPI banding and FISH with probes for the 18-5.8-26S and 5S rDNA genes. The aim of this study was to test for chromosomal changes (chromosome number, karyotype, heterochromatin pattern, rDNA loci) during the evolution of these taxa with respect to their continental relatives. An apparent chromosome stasis was confirmed in all three species, in regards to chromosome number and karyotype morphology. However, there was also evidence of cryptic, cumulative sequence changes. Speciation in these species is not likely associated with large, obvious chromosome rearrangements or polyploidy,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome stasis; Heterochromatin; Ribosomal DNA; Solanum subgen; Leptostemonum. |
Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000200009 |
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Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando. |
Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005 |
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Nogueira,Lídia; Zanoni,Juliani Bruna; Solé,Mirco; Affonso,Paulo Roberto Antunes de Mello; Siqueira,Sérgio; Sampaio,Iracilda. |
Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amphibians; Chromosomes; Fluorochromes; Heterochromatin; RDNA. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156 |
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Dantas,Liliane G.; Guerra,Marcelo. |
A comparative analysis of mitotic chromosomes of Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) was performed aiming to identify cytological differences between the two most important species of this genus. Both species have symmetric karyotypes, with 2n = 20 metacentric chromosomes ranging in size from 2.00 to 1.19 µm (cacao) and from 2.21 to 1.15 mm (cupuaçu). The interphase nuclei of both species were of the arreticulate type, displaying up to 20 chromocentres, which were more regularly shaped in cacao than in cupuaçu. Prophase chromosomes of both species were more condensed in the proximal region, sometimes including the whole short arm. Both species exhibited only one pair of terminal heterochromatic bands, positively stained with chromomycin... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cacao; Cupuaçu; Heterochromatin; Karyotype; RDNA sites; Theobroma. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100017 |
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Marinho,Anne C.T.A.; Vasconcelos,Santelmo; Vasconcelos,Emanuelle V.; Marques,Daniela A.; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina. |
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. Therefore, we analyzed six Jatropha species through fluorochrome staining (CMA/DAPI), fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes and genome size estimation by flow cytometry. Our results revealed several chromosome markers by both CMA/DAPI and FISH for the analyzed species. Five Jatropha species (J. curcas, J. gossypiifolia, J. integerrima, J. multifida and J. podagrica) showed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotaxonomy; DNA C-value; Heterochromatin; Physic nut; RDNA. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300442 |
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Cunha,Marina Souza da; Travenzoli,Natália Martins; Ferreira,Riudo de Paiva; Cassinela,Edson Kuatelela; Silva,Henrique Barbosa da; Oliveira,Francisco Plácido Magalhães; Salomão,Tânia Maria Fernandes; Lopes,Denilce Meneses. |
Abstract The genus Melipona is subdivided into four subgenera based on morphological characteristics, and two groups based on cytogenetic patterns. The cytogenetic information on this genus is still scarce, therefore, the goal of this study was to characterize Melipona paraensis, Melipona puncticollis, and Melipona seminigra pernigra using the following techniques: C-banding, DAPI/CMA3 fluorochromes, and FISH with an 18S rDNA probe. Melipona paraensis (2n=18) and M. seminigra pernigra (2n=22) were classified as high heterochromatin content species (Group II). Their euchromatin is restricted to the ends of the chromosomes and is CMA3+; the 18S rDNA probe marked chromosome pair number 4. Melipona puncticollis (2n=18) is a low heterochromatin content species... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal evolution; DAPI/CMA3 fluorochromes; Fluorescent in situ Hybridization (FISH); Heterochromatin; Meliponini. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500806 |
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Moreira,Camila Nascimento; Di-Nizo,Camilla Bruno; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Ventura,Karen. |
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including chromosome banding and molecular fluorescent in situ hybridization using telomeric sequences and the whole X-chromosome of its sister clade Holochilus brasiliensis (HBR) as probes. A case of remarkable autosomal heteromorphism due to the presence of a whole heterochromatic arm leading to the variability of FN is reported, as well as the occurrence of regions of homology between the X and Y chromosomes... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Fluorescent in situ hybridization; Heterochromatin; Oryzomyini. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200010 |
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Souza,Luiz G.R.; Crosa,Orfeo; Winge,Helga; Guerra,Marcelo. |
The genus Nothoscordum Kunth comprises approximately 20 species native to South America. Karyologically, the genus is remarkable for its large chromosomes and Robertsonian translocations. Variation in chromosome number has been recorded in a few polyploid species and it is unknown among diploids. This study presents the chromosome number and morphology of 53 individuals of seven populations of N. arenarium Herter (2n = 10). In addition, karyotype analyses after C-banding, staining with CMA and DAPI, and in situ hybridization with 5S and 45S rDNA probes were performed in six individuals from one population. All individuals exhibited 2n = 10 (6M + 4A), except for one tetraploid (2n = 20, 12M + 8A) and one triploid (2n = 15, 9M + 6A) plant. C-banding revealed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CMA/DAPI staining; 5S and 45S rDNA; Heterochromatin; Nothoscordum; Robertsonian translocation. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100016 |
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Fregonezi,Jéferson N.; Torezan,José M.D.; Vanzela,André L.L.. |
The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CMA3; FISH; Heterochromatin; Polyploidy; 45S rDNA. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200016 |
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Selivon,Denise; Sipula,Fabiana Maria; Rocha,Lincoln S.; Perondini,André Luiz P.. |
The species of Anastrepha are arranged into 17 intrageneric groups. Recently, it was proposed that two species of the striata group, Anastrepha striata and A. bistrigata, might be realocated to serpentina group. Anastrepha bistrigata and A. serpentina have an X1X2Y/X1X1 X2X2 sex chromosome system while A. striata has a XY/XX system. It was previously proposed that the karyotype of A. bistrigata could be derived from that of A. striata by an Y:A fusion, and that the karyotype of A. serpentina would be derived from another, hypothetical karyotype. In the present report sequential staining with DAPI and chromomycin A3 (CMA3), followed by C-banding, revealed that the C-banded heterochromatic blocks of the sex chromosomes of A. bistrigata have different... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flies; Mitotic chromosomes; Fluorochromes; Heterochromatin. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600009 |
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Fernandes,Thiago; Rego,Letícia do Nascimento Andrade de Almeida; Nardy,Mariana; Yuyama,Priscila Mary; Vanzela,André Luís Laforga. |
The karyotypes of four South American species of Cestrum (C. capsulare, C. corymbosum, C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA+ bands and 45S rDNA were located predominantly in terminal regions. The C-CMA+/DAPI+ bands appeared in interstitial and terminal regions, and the C-DAPI+ bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cestrum; Chromosome banding; FISH; Heterochromatin; Physical maps; 45S and 5S rDNA. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200019 |
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Silva,Guilherme Messias da; Bione,Edgar Guimarães; Cabral-de-Mello,Diogo Cavalcanti; Moura,Rita de Cássia de; Simões,Zilá Luz Paulino; Souza,Maria José de. |
Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome rearrangements; Heterochromatin; Karyotype; NORs; FISH. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012 |
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Registros recuperados: 34 | |
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