SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Raya Montaño, Yurixhi Atenea.

Laelia halbingeriana pertenece a la familia Orchidaceae y debido a su descubrimiento relativamente reciente, no se ha caracterizado morfológica, cromosómica ni molecularmente. Con la finalidad de conocer la diversidad de esta especie y su posible relación con L. anceps, L. superbiens y L. autumnalis, la caracterización molecular mediante el análisis de RAPDs se utilizaron 16 iniciadores, cuatro iniciadores mostraron productos de amplificación aceptable. Se observó 60.97 % de polimorfismo que denota gran variabilidad entre las especies de Laelia. Por otra parte se observó que el número cromosómico más común es de 2n=36. Además, en un tercer estudio se desarrolló una metodología para la propagación in vitro de L. halbingeriana se utilizó el medio de MS...

Palavras-chave: Laelia halbingeriana; Marcadores moleculares; Cromosomas; Micropropagación; Aclimatación; Molecular markers; Chromosomae; Micropropagation; Genética; Doctorado; Acclimation.

Ano: 2013

URL: http://hdl.handle.net/10521/2049

Organogénesis directa de novo en Musa AAA 'Enano gigante' y 'FHIA 23'.

López Barrios, Zeila Yaneth.

Nuestro país se encuentra entre las primeras 12 naciones productoras de banano (Musa sp), y en el ámbito nacional este cultivo ocupa el tercer lugar en importancia, además de estar incluido dentro del rubro de frutas de exportación, que lo convierte en un cultivo altamente rentable. El banano tiene gran demanda en el mercado nacional, con un consumo per cápita de 23 kg anuales, debido a que se encuentra disponible durante todo el año (Daquita, 2006). La Sigatoka negra (Micosphaerella fijiensis) es la principal enfermedad que limita la producción de este frutal. La transformación genética de genotipos es una técnica de vanguardia exitosa que puede permitir incorporar genes que confieran tolerancia a enfermedades por hongos, una vez que se disponga de un...

Palavras-chave: Organogénesis de novo; Zeatina; Cinetina; Thidiazuron; Micropropagación; Zeatin; Kinetin; Micropropagation; Maestría; Fruticultura.

Ano: 2010

URL: http://hdl.handle.net/10521/343

Cultivo in vitro de Stevia rebaudiana Bertoni.

Vázquez Baxcajay, Liberia.

Stevia es un género de plantas fanerógamas perteneciente a la familia de las asteráceas, tiene 407 especies; una de sus especies es Stevia rebaudiana Bertoni, nombrada así por Moisés de Santiago Bertoni. Para el establecimiento in vitro de Stevia rebaudiana se aplicaron cinco tratamientos de desinfestación y se obtuvo al usar Cloro® (1.8 % cloro activo), Ridomil y Fungimycin previo a la siembra in vitro y al agregar al medio Cefotaxima (500 mgL-1) se obtuvo un porcentaje de contaminación de 50 % permitiendo una supervivencia de 47 %. En la inducción de callo se mostro que al combinar 1.5 mgL-1 2,4-D/AIA la capacidad de formación de callo del explante de hoja es de 25 %. El tratamiento de cinetina (1.3 gL-1) en el proceso micropropagación de Stevia mostro...

Palavras-chave: In vitro; Micropropagación; Aclimatación; Stevia; Micropropagation; Acclimatization; Maestría; Fisiología Vegetal.

Ano: 2012

URL: http://hdl.handle.net/10521/751

Germinación y propagación de Tillandsia macdougallii L. B Sm. y Tillandsia violacea Baker (Bromeliaceae) con fines de aprovechamiento sustentable.

Vázquez Hurtado, Nora Berenice.

Los procesos sustentables son adoptados con mayor frecuencia por comunidades rurales de México. La conveniencia de conservar y aprovechar de una forma sostenible los recursos naturales, especialmente las bromelias (con potencial ornamental), pueden generar opciones y beneficios para las familias. Como una opción para evitar el saqueo, así como contribuir al manejo y protección, de las bromelias .En esta investigación se plantearon los siguientes objetivo:1) se evaluó el porcentaje de germinación y viabilidad en las semillas de Tillandsia macdougallii y Tillandsia violacea, 2) se probó el efecto de los tratamientos pregerminativos, AG₃ y NKO₃ en la germinación y desarrollo de plántulas, 3) se estudiaron los efectos de diferentes sustratos para el...

Palavras-chave: Germinación; Viabilidad; Sustratos; Micropropagación; Sobrevivencia; Germination; Viability; Substrates; Micropropagation; Survival; Botánica; Maestría.

Ano: 2014

URL: http://hdl.handle.net/10521/2298

Morfogénesis in vitro de cactáceas.

Gene expression of a putative glutathione S-transferase is responsive to abiotic stress in embryogenic cell cultures of Cyclamen persicum

Téllez Román, Janeth.

En esta investigación se estudiaron las condiciones de cultivo in vitro y las respuestas morfogénicas de tres especies de cactáceas: Echinopsis chamaecereus Friedrich & Glaetzle “aurea”, Gymnocalicium mihanovichii cv. Hibotan y Mammillaria plumosa Weber ornamentales de importancia económica y la última en peligro de extinción. Con la regeneración de brotes, se establecieron protocolos eficientes para la propagación masiva in vitro a partir de la vía organogénica directa e indirecta de E. chamaecereus aurea y M. plumosa. Finalmente, se evaluaron varios tipos de uniones para el establecimiento de microinjertos compuestos de estas dos especies (injertos) e Hylocereus undatus (portainjerto). Durante la micropropagación, los explantes utilizados en todas...

Palavras-chave: Cactácea; Micropropagación; Organogénesis indirecta; Organogénesis directa; Microinjertos; Cactus; Micropropagation; Indirect organogenesis; Direct organogenesis; Micrografts; Fisiología Vegetal; Doctorado.

Ano: 2012

URL: http://hdl.handle.net/10521/1680

Perspectives on the application of biotechnology to assist the genetic enhancement of plantain and banana (Musa spp.)

Hoenemann,Claudia; Ambold,Juliane; Hohe,Annette.

Background: Cyclamen persicum is an economically important ornamental crop that is propagated exclusively through seeds as vegetative propagation using cuttings is not possible. Therefore a micropropagation method through somatic embryogenesis is of high interest; however the method suffers from low reliability concerning quality and quantity of the produced plantlets. A crucial step of the protocol is the removal of plant growth regulators (PGRs) that triggers embryo development. In order to get a better insight in this crucial step of the propagation process, a gene expression analysis has been set up using five different genes of glutathione S-transferases (GST) as these are known to be auxin responsive as well as stress reactive. Results: One out of...

Tipo: Journal article

Palavras-chave: Auxin; Callus; Micropropagation; Semisolid medium; Suspension culture.

Ano: 2012

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100006

Crouch,Jonathan H.; Vuylsteke,Dirk; Ortiz,Rodomiro.

Bananas and plantains (Musa spp.) are the most important tropical fruit crops. They form an integral component of the farming systems in the humid agroecological zones of the tropics. A broad array of applied cell and molecular techniques are increasingly being used worldwide to facilitate and enhance the handling and improvement of plantain and banana germplasm. Tissue culture is used for germplasm exchange, conservation and rapid multiplication, while in vitro seed germination (based on embryo culture or rescue) plays a critical role in generating hybrid plants. DNA marker systems have been developed in Musa to assist germplasm management, selection within the breeding pool or gene introgression from wild species, and for disease diagnosis. Likewise,...

Tipo: Journal article

Palavras-chave: Banana; Improvement; Micropropagation; DNA; Markers; Plantain.

Ano: 1998

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100002

An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis

Sharry,Sandra; Cabrera Ponce,José Luis; Herrera Estrella,Luis; Rangel Cano,Rosa María; Lede,Silvia; Abedini,Walter.

A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight...

Tipo: Journal article

Palavras-chave: Callus induction; Clonal forestry; Embryos conversion; Micropropagation; Persian lilac; Somatic embryos.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300003

Establishment of an in vitro micropropagation protocol for Mecardonia tenella

Alderete,Liliana Marisol; Mori,Marcela; Kato,Adriana; Salvio Escandón,Alejandro.

Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with...

Tipo: Journal article

Palavras-chave: In vitro; Micropropagation; Scrophulariaceae.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Piña-Escutia,José Luis; Vázquez-García,Luis Miguel; Arzate-Fernández,Amaury Martín.

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.

Tipo: Journal article

Palavras-chave: Genetic integrity; Micropropagation; Oceloxóchitl.

Ano: 2010

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007

Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis

In vitro establishment of pepper-rosmarin nodal segments

Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M.

The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant....

Tipo: Journal article

Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid.

Ano: 2005

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010

Horticultura Brasileira

Blank,Arie F; Costa,Andréa S da; Arrigoni-Blank,Maria de Fátima; Mendonça,Aline B de; Ledo,Ana da S.

Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Lippia sidoides; Medicinal plant; Micropropagation; Growth regulator.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000200025

In vitro culture of jambu with different growth regulators

Horticultura Brasileira

Almeida,Susan P; Souza,Joanne MM; Amorim,Andredy MT; Gusmão,Sérgio AL de; Souza,Rodrigo ORM; Santos,Alberdan S.

ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige & Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Acmella oleracea; Micropropagation; Callus induction; BAP; 2.4-D; Nodal segments.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200134

Physiological development of zygotic embryos of heliconias propagated in vitro and conventionally

Horticultura Brasileira

Ulisses,Cláudia; Morais,Marciana; Barbosa,Marta R; Albuquerque,Cynthia C; Willadino,Lilia; Camara,Terezinha R.

ABSTRACT The aim of this study is to compare physiological development of Heliconia bihai cv. Lobster Claw Two plants derived from in vitro culture of zygotic embryos and conventional propagation. Heliconias obtained from rhizomes and from in vitro multiplication were evaluated every 30 days during ten months under greenhouse conditions. The experimental design was completely randomized, with ten repetitions, and the 2x10 factorial arrangement consisted of two plant multiplication methods and ten evaluations performed at different times. The analyzed biometric parameters were plant height, number of leaves, number of tillers, leaf area, and color intensity in the bracts. Plants derived from in vitro culture showed significant differences in the development...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Heliconia bihai; Micropropagation; Rhizome; Vegetative propagation.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200229

Produção de material vegetativo de videira livre de virus no submédio São Francisco.

LEAO, P. C. de S.; MELO, N. F. de; PIRES, E. da L..

Tem como objetivo produzir material vegetativo livre de virus das principais cultivares de videira de interesse comercial para o submedio Sao Francisco, proporcionando aos produtores e viveiristas da Regiao Nordeste do Brasil a possibilidade de disporem de mudas certificadas sadias e de elevada qualidade, evitando a disseminacao de viroses e, com isto, permitindo a implantacao de vinhedos homogeneos e de elevada produtividade.

Tipo: Comunicado Técnico (INFOTECA-E)

Palavras-chave: Videira; Submedio Sao Francisco; Grape; Diseases; Resistance.; Uva; Biotecnologia; Doença; Muda; Meristema; Micropropagação; Resistência; Vírus.; Biotechnology; Meristems; Micropropagation; Seedlings..

Ano: 1997

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/133465

Protocolo de micropropagação para as espécies Piper hispidinervum e P. aduncum.

Efeito de reguladores de crescimento na multiplicação in vitro de brotos de abacaxizeiro.

RAPOSO, A.; TEIXEIRA, R. B.; MANFIO, C. E.; SILVA, D. A. da; MOREIRA, N. R. da S.; MACIEL, S. de A..

Este trabalho apresenta o protocolo de cultivo in vitro para as espécies Piper hispidinervum e P. aduncum, utilizando material oriundo de mudas obtidas por meio da germinação in vitro de sementes.

Tipo: Circular Técnica (INFOTECA-E)

Palavras-chave: Técnicas de laboratorio.; Cultivo in vitro; Micropropagación; Pimenta de macaco.; Micropropagação; Cultura in vitro; Pimenta longa; Piper hispidinervum; Piper longum; Micropropagation; In vitro culture; Laboratory techniques.; Piper aduncum.

Ano: 2012

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/943350

Jardins in vitro : tecnologia e criatividade em pequenos recipientes.

LEMOS, O. F. de; SILVA, S. P. G. da; ALBIM, E. de M. e S.; LAMEIRA, O. A.; REGO, J. C.; MENEZES, I. C. de.

bitstream/item/57407/1/CPATU-BP204.pdf

Tipo: Séries anteriores (INFOTECA-E)

Palavras-chave: Cabeça de onça; Belém; Pará; Brasil; Growth rate.; Abacaxi; Ananás Comosus; Micropropagação; Regulador de Crescimento.; Amazonia; Micropropagation; Pineapples; Varieties..

Ano: 1998

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/374240

Ensaios preliminares para o estabelecimento de um protocolo de assepsia, visando a micropropagação de cultivares de mangueira.

bitstream/item/77595/1/Folder-Jardins-in-vitro-2012.pdf

Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)

Palavras-chave: Ornamental Grass.; Micropropagação; Reprodução vegetal; Planta ornamental; Micropropagation; Plant reproduction..

Ano: 2012

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/951625

CORDEIRO, M. C. R.; CID, L. P. B.; PINTO, A. C. de Q.; GOMES, A. C.; ANDRADE, S. R. M. de; VARGAS RAMOS, V. H..

RESUMO: Visando ao estabelecimento de um protocolo de assepsia para a micropropagação de diferentes cultivares de mangueira, foram coletadas no campo, por um período de dois anos (2000-2001), microestacas e folhas jovens e tratadas no laboratório com hipoclorito de sódio (v/v) 2% com Tween 80 a 0,025% por 15 minutos no caso de microestacas e, 0,5; 1,0; 1,5 e 2% com Tween 80 a 0,06 por 5 minutos no caso de folhas jovens. As microestacas, depois da assepsia com hipoclorito de sódio foram tratadas também com Benomyl 200mg/L por 30 minutos, ficando, em seguida, em magentas com vermiculita umedecidas em água estéril. As folhas jovens depois da assepsia foram colocadas em placas de Petri, contendo meio nutritivo básico SP com polivinilpirrolidona, Benomyl e...

Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)

Palavras-chave: Mango; Desinfectants.; Cultura de Tecido; Desinfecção; Explante; Manga; Mangifera Indica; Micropropagação; Reprodução Vegetal.; Explants; Plant propagation; Micropropagation; Tissue culture..

Ano: 2002

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/555088

Micropropagação da mandioca mediante ápices caulinares e segmentos nodais.