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| Registros recuperados: 45 | |
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| McKinney,Emily A.; Oliveira,Marcos T.. |
| The field of mitochondrial DNA (mtDNA) replication has been experiencing incredible progress in recent years, and yet little is certain about the mechanism(s) used by animal cells to replicate this plasmid-like genome. The long-standing strand-displacement model of mammalian mtDNA replication (for which single-stranded DNA intermediates are a hallmark) has been intensively challenged by a new set of data, which suggests that replication proceeds via coupled leading-and lagging-strand synthesis (resembling bacterial genome replication) and/or via long stretches of RNA intermediates laid on the mtDNA lagging-strand (the so called RITOLS). The set of proteins required for mtDNA replication is small and includes the catalytic and accessory subunits of DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA replication; Mitochondria; MtSSB; Pol γ; Twinkle. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300002 |
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| Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia. |
| We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005 |
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| Viblanc, Vincent A.; Schull, Quentin; Roth, Jeffrey D.; Rabdeau, Juliette; Saraux, Claire; Uhlrich, Pierre; Criscuolo, François; Dobson, F. Stephen. |
| 1.Oxidative stress has been proposed as a central causal mechanism underlying the life history trade-off between current and future reproduction and survival in wild animals. 2.Whereas mixed evidence suggests that maternal oxidative stress may act both as a constraint and a cost to reproduction, some studies have reported a lack of association between reproduction and maternal oxidative stress. 3.The oxidative shielding hypothesis offers an alternative explanation, suggesting that mothers may pre-emptively mitigate the oxidative costs of reproduction by increasing antioxidant defences prior to reproduction. 4.We tested the oxidative constraint, cost, and shielding hypotheses using a longitudinal field study of oxidative stress levels in a species that... |
| Tipo: Text |
Palavras-chave: Antioxidant; Body condition; Cost of reproduction; Life history; Mammal; Mitochondria; Trade-off. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00415/52679/53542.pdf |
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| Kraffe, E; Tremblay, R; Belvin, S; Le Coz, Jean-rene; Marty, Y; Guderley, H. |
| In scallops, gametogenesis and spawning can diminish the metabolic capacities of the adductor muscle and reduce escape response performance. To evaluate potential mechanisms underlying this compromise between reproductive investment and escape response, we examined the impact of reproductive stage (pre-spawned, spawned and reproductive quiescent) of the giant scallop, Placopecten magellanicus, on behavioural (i.e., escape responses), physiological (i.e., standard metabolic rates and metabolic rates after complete fatigue) and mitochondrial capacities (i.e., oxidative rates) and composition. Escape responses changed markedly with reproductive investment, with spawned scallops making fewer claps and having shorter responses than pre-spawned or... |
| Tipo: Text |
Palavras-chave: Placopecten magellanicus; Sterols; Fatty acids; Plasmalogens; Phospholipids; Cytochrome C oxidase; Metabolic rate; Escape response; Muscle; Scallops; Reproduction; Mitochondria. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4786.pdf |
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| Dudognon, Tony; Soudant, Philippe; Seguineau, Catherine; Quere, Claudie; Auffret, Michel; Kraffe, Edouard. |
| Sessile animals that live on the foreshore undergo tidal cycles, and have to face variations in physical and chemical parameters such as oxygen concentration. During emersion, availability of dissolved oxygen can be lowered for bivalves, which have only a small reserve of seawater inside their closed shell. Differences in oxygen concentration are thus expected to lead to modifications of the metabolism, including changes in mitochondrial activity. Previous studies investigated air exposure under extreme conditions, which do not always reflect environmental conditions these invertebrates have to cope with. In this study, oxidative capacities of gill mitochondria of the oyster Crassostrea gigas were studied during a tidal cycle period, by comparing oysters... |
| Tipo: Text |
Palavras-chave: Mitochondria; Oxygen consumption; ATP production; Respiratory chain inhibitor; Crassostrea gigas. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26854/24981.pdf |
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| Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
| Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
| Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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| Grunau, Christoph; Voigt, Susanne; Dobler, Ralph; Dowling, Damian K.; Reinhardt, Klaus. |
| Cytoplasmic components and their interactions with the nuclear genome may mediate patterns of phenotypic expression to form a joint inheritance system. However, proximate mechanisms underpinning these interactions remain elusive. To independently assess nuclear genetic and epigenetic cytoplasmic effects, we created a full-factorial design in which representative cytoplasms and nuclear backgrounds from each of two geographically disjunct populations of Drosophila melanogaster were matched together in all four possible combinations. To capture slowly-accumulating epimutations in addition to immediately occurring ones, these constructed populations were examined one year later. We found the K4 methylation of histone H3, H3K4me3, an epigenetic marker... |
| Tipo: Text |
Palavras-chave: Epigenetics; Mitochondria; Mito-nuclear coevolution; Wolbachia. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00478/58935/62427.pdf |
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| Motoyama,Aracy A.; Watanabe,Ii-sei; Ogawa,Koichi. |
| The characteristics of intrinsic muscle fibers in the anterior third of the adult rat tongue were studied employing light microscopy, SEM and high-resolution SEM (HRSEM) methods. For light microscopy, specimens were fixed in Bouin's solution and embedded in paraffin. In order to identify the muscle fibers and bundles of collagen fibers, frontal sections were stained with hematoxylin-eosin, azo-carmin and picro-sirius. The results showed that muscle fibers near the lamina propria are fixed with connective tissue, constituting several groups. The bundles of muscle fibers are arranged in longitudinal, vertical and transversal directions. For SEM, specimens were fixed in modified Karnovsky solution and freeze-fractured in liquid nitrogen revealed the collagen... |
| Tipo: Journal article |
Palavras-chave: Muscle fibers; Tongue; Rat; HRSEM; Mitochondria; Basement membrane. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400006 |
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| Battah,Khairat A; Badran,Darwish H; Shraideh,Ziad A. |
| The liver is one of the major organs that is indirectly affected by cigarette smoke. The aim of this project is to define the histologic and ultrastructural changes in normal liver cells after exposing animals to cigarette smoke. Thirty albino rats were exposed to cigarette smoke for 90 days, followed by morphologic examination of their livers under light microscope and electron microscope. The liver cells of cigarette smoke exposed rats showed mild swelling with increased eosinophilia. Ultrastructural examination of these cells demonstrated cytoplasm with highly proliferated and crowded mitochondria. There were many electron dense mitochondria. These mitochondria were pleomorphic in shape compared to mitochondrias of control rats. Also, loss of... |
| Tipo: Journal article |
Palavras-chave: Liver; Smoking; Mitochondria; Ultrastructure. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400011 |
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| Grajeda y Ortega,María de los Angeles; Ortiz Ordoñez,Esperanza; Favari,Liliana; Shibayama,Mineko; Silva Olivares,Angélica; López López,Eugenia. |
| The effects of sublethal concentrations of cadmium (0.64 µg/L), iron (0.043 mg/L) and zinc (0.31 mg/L) and a mixture of these metals on succinate dehydrogenase (SD) and alkaline phosphatase (AP) activity and on structural changes in the mitochondria of epithelium cells of the digestive tract were examined in the oligochaete Limnodrillus hoffmeisteri after 96 h of exposure in artificial sediments. SD activity was significantly inhibited, particularly in treatments with Cd alone (92.57%), while AP increased its activity with Cd alone (73.23%). However, when this metal was mixed with Fe and Zn, the inhibition of SD activity was lower (67.82%) than with Cd alone, showing an antagonistic effect and AP increased its activity (73.26%). Mitochondria were... |
| Tipo: Journal article |
Palavras-chave: Heavy metals; Mixture; Alkaline phosphatase; Succinate dehydrogenase activity; Limnodrillus hoffmeisteri; Epithelium cells; Mitochondria. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200018 |
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| Masatani, Tatsunori; Asada, Masahito; Hakimi, Hassan; Hayashi, Kei; Yamagishi, Junya; Kawazu, Shin-ichiro; Xuan, Xuenan. |
| Cysteine-based peroxidases, known as peroxiredoxins (Prx) or thioredoxin peroxidases (TPx), are important antioxidant enzymes that prevent oxidative damage caused by reactive oxygen species (ROS). In this study, we identified a novel mitochondrial 2-Cys Prx, BbTPx-2, from a bovine Babesia parasite, B. bovis. BbTPx-2 complementary DNA (cDNA) encodes a polypeptide of 254 amino acid residues. This protein has a mitochondrial targeting peptide at the N-terminus and two conserved cysteine residues of the typical 2-Cys Prx. By using a thiol mixed-function oxidation assay, the antioxidant activity of recombinant BbTPx-2 was revealed, and its antioxidant activity was comparable to that of a cytosolic 2-Cys Prx from B. bovis, BbTPx-1. Notably, we confirmed that... |
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Palavras-chave: Antioxidant activity; Babasia bovis; Mitochondria; Peroxiredoxin. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4487 |
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| Smaili,Soraya; Hirata,Hanako; Ureshino,Rodrigo; Monteforte,Priscila T.; Morales,Ana P.; Muler,Mari L.; Terashima,Juliana; Oseki,Karen; Rosenstock,Tatiana R.; Lopes,Guiomar S.; Bincoletto,Claudia. |
| Transient increase in cytosolic (Cac2+) and mitochondrial Ca2+ (Ca m2+) are essential elements in the control of many physiological processes. However, sustained increases in Ca c2+ and Ca m2+ may contribute to oxidative stress and cell death. Several events are related to the increase in Ca m2+, including regulation and activation of a number of Ca2+ dependent enzymes, such as phospholipases, proteases and nucleases. Mitochondria and endoplasmic reticulum (ER) play pivotal roles in the maintenance of intracellular Ca2+ homeostasis and regulation of cell death. Several lines of evidence have shown that, in the presence of some apoptotic stimuli, the activation of mitochondrial processes maylead to the release of cytochrome c followed by the activation of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calcium; Apoptosis; Bax; Mitochondria; Endoplasmic reticulum; Neurodegeneration and aging. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300011 |
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| Registros recuperados: 45 | |
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