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Gill tissues of the mussel Mytilus edulis chilensis: A new source for antimicrobial peptides Electron. J. Biotechnol.
Mercado,Luis; Schmitt,Paulina; Marshall,Sergio H; Arenas,Gloria.
Antimicrobial peptides are small-sized, cationic and amphipathic molecules able to neutralize pathogenic microorganisms. Their antimicrobial effects tie them to mechanisms of immune defense, which is why they have been normally purified from immune cells. We describe an apparently new group of antimicrobial peptides from gill tissues of the mussel Mytilus edulis chilensis. 20 specimens yielded 40 g of gills which produced 16 mg of an enriched fraction with antimicrobial activity as low as 0.045 µg/µl over reference strains. Considering the chemical nature of these molecules we used an acid extraction procedure followed by consecutive cationic exchange and hydrophobic interaction chromatography steps for peptide enrichment. The resulting...
Tipo: Journal article Palavras-chave: Antimicrobial peptides; Biochemical characterization; Gill tissues; In vitro activity; Mussels.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300008
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Сравнительная оценка степени загрязнения мидий и донных осадков акваторий Карадагских и Севастопольских бухт хлорорганическими соединениями IBSS Repository
Жерко, Н. В.; Малахова, Л. В.; Бочко, О. Ю..
В 1981-1991 годах проводили экологические исследования в заповедной зоне Карадага и Севастопольских бухтах. Изучали степень загрязнения мидий (Mytilus galloprovincialis) и донных осадков полихлорированными бифенилами и хлорорганическими пестицидами. Полученные данные свидетельствуют о различной степени загрязнения этими соединениями исследуемых бухт. Наблюдения за содержанием хлорорганических соединений, проведенные в последние годы, показали тенденцию к снижению концентраций ПХБ и пестицидов в изучаемых объектах.
Tipo: Journal Contribution Palavras-chave: Черное море; Хлорорганические пестициды; Полихлорированные бифенилы; Донные осадки; Мидии; The Black Sea; Chlororganic pesticides; Mussels; Polychlorated biphenils.
Ano: 2002 URL: http://repository.ibss.org.ua/dspace/handle/99011/416
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Результаты гидролого-гидрохимического мониторинга мидийной фермы в прибрежной зоне южного берега Крыма (бухта Ласпи) IBSS Repository
Куфтаркова, Е. А.; Щуров, С. В.; Родионова, Н. Ю..
Results of the hydrological-hydrochemical monitoring carried out in 2007–2009 at the mytilus farm are given. The lowered salinity, nutrients and the raised pH in comparison with the period 1983–1987 are caused by prevalence of downwelling circulation and insufficient dynamic activity of waters.
Tipo: Book Section Palavras-chave: Mussels; Hydrochemical structure; Circulation of waters.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1852
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Потоки нефтяных углеводородов через поселение мидий, обитающих на южном молу Севастопольской бухты (Чёрное море). IBSS Repository
Соловьёва, О. В..
На южном молу Севастопольской бухты численность мидии на бетонных тетраподах 380±99 экз./м2, на каменной наброске – 128±71 экз./м2. Объём воды, фильтруемой мидиями, обитающими на подводной части исследуемого мола составляет 53 тыс.м3/сут, что позволяет за сутки отфильтровать 3.3 кг нефти.
Tipo: Journal Contribution Palavras-chave: Мидии; Биофильтр; Гидротехнические сооружения; Нефтяные углеводороды; Чёрное море; Mussels; Biofilter; Hydraulic engineering structures; Petrol hydrocarbons; Black Sea; Мідії; Біофільтр; Гідротехнічні спорудження; Нафтові вуглеводні; Чорне море.
Ano: 2007 URL: http://repository.ibss.org.ua/dspace/handle/99011/858
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Активность ферментов пероксидного комплекса тканей мидии Mytilus galloprovincialis Lam в норме и в условиях естественного окислительного стресса IBSS Repository
Гостюхина, О. Л.; Солдатов, А. А.; Головина, И. В..
Исследовано содержание продуктов перекисного окисления липидов (ПОЛ) и состояние ферментативного пероксидного комплекса (ПК) (глутатионпероксидазы (ГП), каталазы и пероксидазы) в гепатопанкреасе, жабрах и ноге черноморского моллюска Mytilus galloprovincialis Lam. в условиях физиологического покоя и нереста. Последнее рассматривали как модель естественного окислительного стресса. Изучали особей двух цветовых морф - черной и коричневой. Выявлена тканевая специфика активностей ферментов и распределения продуктов ПОЛ в состоянии нормы, а также особенности ответных реакций тканей моллюсков обеих морф при действии окислительной нагрузки. Наиболее чувствительной к действию перекисных продуктов в условиях естественного окислительного стресса оказались особи черной...
Tipo: Journal Contribution Palavras-chave: Ферменты; Каталаза; Пероксидаза; Глутатионпероксидаза; Перекисное окисление липидов; Окислительный стресс; Мидии; Цветовые морфы; Нерест; Enzymes; Catalase; Peroxidase; Glutathione peroxidase; Lipid peroxidation; Spawning; Oxidative stress; Mussels; Color morphs Spawning Enzymes Lipids Spawning Enzymes Lipids http://aims.fao.org/aos/agrovoc/c_14490 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_4362.
Ano: 2005 URL: http://repository.ibss.org.ua/dspace/handle/99011/226
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Вертикальное распределение моллюсков Mytilus Galloprovincialis Lam. и Mytilaster lineatus Gmel. на каменной наброске гидротехнического сооружения IBSS Repository
Соловьёва, О. В..
The number and size of mussels Mitilus Galloprovincialis Lam. and Mytilaster Lineatus Gmel. on the riprap of a hydraulic structure (southern pier of Sevastopol bay) was analyzed. It is shown that at this riprap, the distribution of mussels over depth is more determined by abundance of mytilidae larva in the water column, and less – by hydrodynamic effects.
Tipo: Book Section Palavras-chave: Mussels; Mytilidae; Hydrotechnical buildings; Hydraulic engineering.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1890
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Концентрация ртути в воде, донных отложениях и мидии Mytilus galloprovincialis Lam. на шельфе Крыма (Черное море) IBSS Repository
Рябушко, В. И.; Козинцев, А. Ф.; Костова, С. К.; Парчевская, Д. С.; Шинкаренко, В. К..
Исследовано содержание ртути в мягких тканях и раковинах мидий Mytilus galhprovinciulis Lam. в зависимо­сти от индивидуального возраста моллюсков из акваторий мыса Тарханкут, Карадагского природного запо­ведника, г. Ялты, в т.ч. глубоководного выброса хозяйственно-бытовых и сточных вод. Линейные размеры раковины мидии в зависимости от их возраста хорошо апроксимируются степенной функцией, а концентра­ция ртути в теле и раковине - квадратичной параболой. Акватория г. Ялты характеризуется высоким уровнем загрязнения мидий ртутью (выше предельно-допустимой концентрации, установленной требованиями сани­тарных норм для пищевых продуктов), начиная с самых мелких животных. Фоновые значения содержания Hg в воде во всех районах исследований ниже ПДК для природных...
Tipo: Journal Contribution Palavras-chave: Ртуть; Вода; Донные отложения; Мидия; Черное море; Mercury; Water; Bottom sediment; Mussels; Black Sea Mercury Bottom water Sediments Mercury http://aims.fao.org/aos/agrovoc/c_4751.
Ano: 2005 URL: http://repository.ibss.org.ua/dspace/handle/99011/221
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Сезонная динамика личинок двустворок и их вертикальное распределение в прибрежном планктоне внешнего рейда Севастопольской бухты (Черное море) IBSS Repository
Казанкова, И. И..
Проанализирована сезонная динамика численности личинок массовых видов Bivalvia, в том числе Anadara inaequivalvis - недавнего вселенца в Черное море. Весной отмечена высокая концентрация велигеров мидий. Пик численности поздних великонх мидий наблюдали в декабре - вблизи берега и в марте - в удалении от берега в горизонте 10 - 25 м. Выявлена неравномерность в вертикальном распределении личинок Bivalvia, благодаря чему возможно их использования в качестве идентификаторов водных масс.
Tipo: Journal Contribution Palavras-chave: Сезонная динамика; Мидии; Seasonal dynamics; Mussels.
Ano: 2002 URL: http://repository.ibss.org.ua/dspace/handle/99011/410
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Межгодовая изменчивость возрастной структуры популяции мидий Mytilus galloprovincialis в северо-западной части Черного моря IBSS Repository
Шурова, Н. М..
Проведен анализ многолетней изменчивости размерно-возрастной структуры поселений мидий в северо-западной части Черного моря. Выявленные межгодовые различия свидетельствуют о том, что в современных экологических условиях на северо-западном шельфе Черного моря продолжаются негативные процессы, которые увеличивают смертность моллюсков и приводят к неуклонному омоложению возрастной структуры их поселений. Это вызывает уменьшение продолжительности жизни черноморской мидии и снижение ее репродукционного потенциала.
Tipo: Journal Contribution Palavras-chave: Population; Mussels; Long-term changes; The Black Sea; Популяция; Мидии; Черное море Population structure Population structure http://aims.fao.org/aos/agrovoc/c_6115.
Ano: 2003 URL: http://repository.ibss.org.ua/dspace/handle/99011/489
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Management of oyster and mussel culture ArchiMer
Heral, Maurice; Drinkwaard, A.; Bohle, B.; Figueras, Antonio; Lavoie, R.; Maurer, Daniele.
The world aquaculture production (fresh and marine waters) achieved 11.1 million tonnes in 1986 (FAO, 1989). The molluscs represented 21 % of the total production. Marine aquaculture with 5.45 million tonnes reached 49% of the total production. The marine cultivated mollusc production was 2.25 million tonnes due mainly to oysters (36%), mussels (34%), clams (16%), and scallops (6%). As the aquaculture production is clearly identified in the statistics only since 1984, the evolution of the production of oysters and mussels for the last 15 years has been analyzed with the FAO data for the total catch (fisheries and culture). By comparison of the two statistics for the same year (1986), the fisheries represented only 17% for oysters and 16% for mussels. Fig....
Tipo: Text Palavras-chave: Culture; Mussels; Oysters; Panel report.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-3020.pdf
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Relations entre apports terrigènes et conchyliculture dans les Pertuis Charentais ArchiMer
Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Guesdon, Stephane; Bechemin, Christian.
This work presents interactions between quantitative and qualitative river freshwater inputs and the shellfish farming (oyster and mussel) in the Pertuis Charentais. The quantity of freshwater (i.e. salinity) seems to have a weak influence on the shellfish farming contrarily to its quality determined by particulate and dissolved matters contained in the water. In autumn and winter, large precipitations have a "globally positive" effect amending the coastal ecosystem. Associated dissolved nutriments and the organic matter largely determine the quality of the coming spring growth for bred shellfish, itself controlling in turn the annual yield efficiencies. However, in winter their effects are postponed because of strong mineral load, low luminosity and...
Tipo: Text Palavras-chave: Conchyliculture; Huîtres; Moules; Apports terrigènes; Zone côtière; Pertuis Charentais.; Shellfish aquaculture; Oysters; Mussels; Terrestrial inputs; Coastal zone; Pertuis Charentais..
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35964/34490.pdf
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Genome mapping in shellfish aquaculture species ArchiMer
Lapegue, Sylvie.
The development of fish and shellfish farming is facing several challenges linked to the infancy of the industry: i.e. domestication, genetic improvement of disease resistance, genetic improvement of feed efficiency to fish meal and fish oil substitution by vegetal products, development of adapted vaccines, supply of fry or spat at high quality and high robustness, adaptation to environmental change, production of healthy, high quality consumer products and limitation of environmental impact of the activity and escapees. Over recent years, the EU and individual countries have supported the development of genomic tools for some aquaculture species (e.g. rainbow trout, sea bass, sea bream, turbot pacific oyster, Atlantic salmon). New perspectives arise...
Tipo: Text Palavras-chave: Mussels; Oysters; Domestication; Genome mapping; Genetic.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3819.pdf
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The mass mortality of blue mussels (Mytilus spp) from the Atlantic coast of France is associated with heavy genomic abnormalities as evidenced by flow cytometry ArchiMer
Benabdelmouna, Abdellah; Ledu, Christophe.
Since 2014, France’s blue mussel industry has been facing heavy mortality outbreaks (90-100%) affecting both juveniles and adults. This report presents evidence of heavy genomic abnormalities associated with mortality outbreaks in blue mussels, Mytilus edulis-galloprovincialis, from the Atlantic coast of France. In this study, ploidy characteristics of hemic cells were investigated using Flow CytoMetry (FCM), revealing an unusual, broad continuum of ploidy distribution from hypodiploidy to tetraploidy. FCM was additionally used to evaluate, at individual and populations levels, different thresholds of genomic abnormality (GA%) using the percentage of non-diploid nuclei. Individual mussels were considered to be abnormal when more than 10% of hemocytes in...
Tipo: Text Palavras-chave: Mussels; Mortality; Cytogenetic quality; Hemocytes; Ploidy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00341/45171/44566.pdf
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Les activités de production - conchyliculture ArchiMer
Deschamps, Gerard; Miossec, Dominique; Pannier, Thierry; Huet, Jerome; Lorance, Pascal.
These studies of halieutic ecology produced at the request of Electricité de France by the French Institute of Research for Exploitation of the Sea in the Normand Breton Gulf must constitute one of the elements making it possible to come to a conclusion or not on the feasibility of the tidal power plant construction project; these studies have three parts: The first part discusses halieutic zooplankton. In the first place, the main species that constitute the exploitable living resources are considered, observed from the first stages of their life, in the egg or at different larval stages preceding the juvenile state. Also of interest are certain holoplanktonic groups. The whole of these observations establishes the status of zooplanktonic populations...
Tipo: Text Palavras-chave: Whelk; Mussels; Oysters; Shellfish farming; Professional fishing; Holoplankton; Zooplankton; France; Conchyliculture; Buccin; Pêche professionnelle; Usine marémotrice; Zooplancton; Holoplancton; Ecologie Halieutique.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/rapport-2333.pdf
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Contamination de moules (Mytilus sp.) en milieu marin par des substances pharmaceutiques et produits de soin ArchiMer
Bachelot, Morgane.
Pharmaceutical and personal care products used for human health protection are detected in all aquatic ecosystems. Among them, some compounds have physico-chemical or pharmacological caracteristics that suggest a bioaccumulation capability. It’s the case of the UV filters, EHMC and OC, with log Kow higher than 5 or pharmaceuticals like diazepam and tetrazepam, with log Kow higher than 2.82. The aim of these Phd works was to assess marine mussels contamination for UV filters and benzodiazepines. So, a new analytical method was developed and validated for the determination of these compounds in mussels. EHMC and OC analysis realized in mussels sampled in french coastal waters showed that mainly contamination came from recreational activities in summer...
Tipo: Text Palavras-chave: Benzodiazépines; Filtres UV; Médicaments; Moules; Contamination marine; Surveillance des eaux côtières; Benzodiazepine; UV filters; Pharmaceuticals; Mussels; Marine contamination; Coastal water monitoring.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12259/9028.pdf
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Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais ArchiMer
Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf
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Estimation des stocks de moules dans le Pertuis Breton en 1987 ArchiMer
Mazurie, Joseph; Dardignac-corbeil, Marie-jose.
Une estimation de la biomasse de moules présente sur les bouchots du Pertuis Breton situés au sud de la Sèvre niortalse a été réalisée en 1987 . Le but était de mettre au point une stratégie d'échantillonnage adaptée à ce mode de culture . La méthode utilisée est décrite et une optimisation de l'effort d'échantillonnage réalisée ; elle détermine pour différentes précisions l'effort à consentir. La biomasse étudiée a été estimée à 4 722 tonnes avec une précision de 14 %.
Tipo: Text Palavras-chave: Moules; Bouchots; Biomasse; Echantillonnage; Pertuis Breton; Mussels; Biomass; Sampling; Pertuis Breton.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00045/15589/12991.pdf
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Estimation du stock de moules sur bouchots en baie du Mont Saint-Michel ArchiMer
Gerard, Denis.
The quantity of mussels cultivated on wooden poles called « bouchots », in Mont Saint-Michel bay, has been estimated in april 2002, by volume, number and weight. To do so, a sampling strategy has been conceived, operated and then optimized, taking into account the spatial distribution of this stock and the material and human constraints (especially the amount of persons and working days available). The sampling plan uses procedures of stratification, degrees, regression, so as to reach the optimum precision related to cost. The operation took a great advantage of the efficient help of mussel farmers. During the first day, the task consisted to estimate the number and position of poles occupied by mussels, with the help of 5 persons on boat. The following 2...
Tipo: Text Palavras-chave: Mont Saint Michel; Sampling; Biomass; Stock; Mussels; Mont Saint Michel; Échantillonnage; Biomasse; Bouchot; Stock; Moules.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/rapport-5304.pdf
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Chemical environment of the hydrothermal mussel communities in the Lucky Strike and Menez Gwen vent fields, Mid Atlantic ridge ArchiMer
Sarradin, Pierre-marie; Caprais, Jean-claude; Riso, Ricardo; Kerouel, Roger; Aminot, Alain.
Water samples were collected around the communities of a hydrothermal mussel, Bathymodiolus sp., in the Lucky Strike (1700 m depth) and Menez Gwen (850 m depth) areas, Mid Atlantic Ridge, and analysed for chemical constituents. The environment surrounding the organisms consists in sea water (88 to 100%) mixed with hydrothermal fluid with pH between 6.2 and 8, Sigma S (H2S + HS-) concentrations from 0 to 62 mu mol l(-1). High dissolved organic carbon (DOC) concentrations suggest a highly productive ecosystem. Production of ammonium and DOG, consumption of nitrate and sulfide in the vicinity of the organisms are among the environmental changes found in relation with local biological activity. Concentrations of Cu and Pb were high, implying that the organisms...
Tipo: Text Palavras-chave: Environment; Sea water; Hydrothermal vents; Mussels.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00147/25847/23968.pdf
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Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid ArchiMer
Litaker, R. Wayne; Stewart, Thomas N.; Eberhart, Bich-thuy L.; Wekell, John C.; Trainer, Vera L.; Kudela, Raphael M.; Miller, Peter E.; Roberts, Alice; Hertz, Cassandra; Johnson, Tyler A.; Frankfurter, Greg; Smith, G. Jason; Schnetzer, Astrid; Schumacker, Joe; Bastian, Jonnette L.; Odell, Anthony; Gentien, Patrick; Le Gal, Dominique; Hardison, I. Ransom; Tester, Patricia A..
Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting. diarrhea, and in more severe cases confusion. loss of memory. disorientation. and even coma or death. This paper describes the development and validation of a rapid. sensitive. enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography. fluorenylmetbox-yearbonyl high performance liquid chromatography, or liquid chromatography-mass spectrometry methods. It has a linear range from 1.1-3 ppb and was used successfully to measure DA...
Tipo: Text Palavras-chave: Test kit; Razor clams; Scallops; Mussels; ELISA; Domoic acid poisoning; ASP.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6253.pdf
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