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Sistema cromatográfico miniaturizado para la determinación de coenzima Q10 en plasma, músculo y plaquetas ABCL
Contin,Mario; Martinefski,Manuela; Lucangioli,Silvia; Tripodi,Valeria.
La coenzima Q10 (CoQ10) es un componente esencial de la cadena respiratoria que interviene en la producción celular de energía. A su vez, es considerada un potente antioxidante que actúa en la prevención del daño oxidativo del ADN, membranas biológicas y lipoproteínas. En los últimos tiempos se ha demostrado que su deficiencia está implicada en varias patologías como enfermedades mitocondriales, cardíacas y musculares, cáncer y durante el tratamiento con estatinas. Como la terapia con CoQ10 resulta beneficiosa en dichas patologías, sus niveles deben ser monitoreados durante el tratamiento. La determinación de los niveles de CoQ10 para el diagnóstico de su deficiencia y control post tratamiento representa un gran desafío analítico puesto que es una molécula...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Coenzima Q10; Sistema miniaturizado de cromatografía líquida de alta resolución; Plaquetas; Músculo; Plasma.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000200004
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Balanço de nitrogênio e estimativas de perdas endógenas em vacas lactantes alimentadas com dietas contendo palma forrageira e teores crescentes de uréia e mandioca - DOI: 10.4025/actascianimsci.v28i1.848 Animal Sciences
Soares Cruz, Maria Cláudia; CEFET-Petrolina; Véras, Antonia Sherlânea Chaves; UFRPE; Ferreira, Marcelo de Andrade; UFRPE; Batista, Angela Maria Vieira; UFRPE; Santos, Djalma Cordeiro dos; IPA; Coelho, Marcelo Iran de Souza; CEFET-Pernambuco.
Foram avaliados os efeitos da substituição do farelo de soja pela mistura uréia mais mandioca, em rações constituídas de palma, silagem de sorgo e concentrado, sobre concentrações de uréia, balanço de nitrogênio (N) e estimativas de perdas endógenas totais em 8 vacas Girolando em lactação, distribuídas em dois quadrados latinos simultâneos (4x4). As concentrações de uréia e N-uréico no plasma e leite, em mg/dL e N-uréico no leite (g/dia) não foram afetadas pelo aumento do nível de uréia na ração, apresentando médias de 31,37; 14,62; 37,43; 17,44; 5,37. As excreções urinárias de uréia e N-uréico também não foram influenciadas, com médias de 249,45 e 116,24 mg/kg PV. Igualmente, o balanço de N não foi afetado pelos tratamentos, cujos valores médios foram de...
Palavras-chave: 5.04.00.00-2 Zootecnia bovinos; Leite; Plasma; Uréia; Urina. 5.04.00.00-2 Zootecnia.
Ano: 2006 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/848
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Plasma miR-1273g-3p acts as a potential biomarker for early Breast Ductal Cancer diagnosis Anais da ABC (AABC)
Guo,Huan; Zeng,Xiangting; Li,Haining; Guo,Yanxiang; Wang,Tao; Guo,Hongyun; Zhu,Gongjian; Wang,Lan; Zhou,Haihong; Liu,Kedan; Chen,Xiaoyi; Wang,Haitao; Zhao,Xinke; Su,Haixiang; Li,Yingdong.
Abstract Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer’s disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast ductal carcinoma; Plasma; MiR-1273g-3p; Circulating miRNA; Biomarker.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100801
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Cálcio, fósforo e proteína total no sangue de frangos de corte em função de níveis de balanço eletrolítico da ração Arq. Bras. Med. Vet. Zootec.
Vieites,F.M.; Fraga,A.L.; Moraes,G.H.K.; Vargas Júnior,J.G.; Nalon,R.P.; Corrêa,G.S.S.; Nunes,R.V..
Determinaram-se os melhores valores do balanço eletrolítico (BE) de dietas para frangos quanto aos teores de cálcio, fósforo e proteína total no sangue de frangos de corte aos 42 dias de idade. Foram utilizadas 1728 aves de marca comercial, machos. As aves foram alimentadas com duas dietas basais, 20 ou 23% de proteína bruta (PB), na fase inicial, e uma única ração basal, 20% de PB, na fase de crescimento, combinadas com níveis de balanço eletrolítico de 0; 50; 100; 150; 200; 250; 300 e 350mEq/kg. Foi utilizado delineamento experimental inteiramente ao acaso, em arranjo fatorial, com seis repetições de 18 aves cada e 96 unidades experimentais. Foi escolhida uma ave em cada unidade experimental para a coleta de sangue. Foram avaliados os teores de cálcio,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicultura industrial; Equilíbrio eletrolítico; Homeostasia; Minerais; Plasma.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400014
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Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents BABT
Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035
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Chromosome damage in underground coal miners: detection by conventional cytogenetic techniques and by submitting lymphocytes of unexposed individuals to plasma from at-risk groups Braz. J. Genet.
Agostini,J.M.S.; Otto,P.A.; Wajntal,A..
Chromosome abnormalities and the mitotic index in lymphocyte cultures and micronuclei in buccal mucosa cells were investigated in a sample of underground mineral coal miners from Southern Brazil. A decreased mitotic index, an excess of micronuclei and a higher frequency of chromosome abnormalities (fragments, polyploidy and overall chromosome alterations) were observed in the miners when compared to age-paired normal controls from the same area. An alternative assay for clastogenesis in occupational exposition was tested by submitting lymphocytes from non-exposed individuals to a pool of plasmas from the exposed population. This assay proved to be very convenient, as the lymphocytes obtained from the same individuals can be used as target as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome damage; Underground coal miners; Cytogenetic; Lymphocytes; Plasma; At-risk groups.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400021
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Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder BJMBR
Song,Y.R.; Wu,B.; Yang,Y.T.; Chen,J.; Zhang,L.J.; Zhang,Z.W.; Shi,H.Y.; Huang,C.L.; Pan,J.X.; Xie,P..
Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolar disorder; Plasma; Proteomic; Carbonic anhydrase 1; Apolipoprotein.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100973
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Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA BJMBR
Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A..
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019
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Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens BJMBR
Veloso,D..
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 <FONT FACE="Symbol">®</FONT> stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004
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Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment BJMBR
Turell,L.; Carballal,S.; Botti,H.; Radi,R.; Alvarez,B..
Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~80% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thiol; Sulfhydryl; Albumin; Sulfenic acid; Mixed disulfides; Plasma.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400001
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Development of a new HPLC method for in vitro and in vivo studies of haloperidol in solid lipid nanoparticles BJPS
Yasir,Mohd; Sara,Udai Vir Singh; Som,Iti.
ABSTRACT A simple and sensitive HPLC method was developed and validated for the quantification of haloperidol in solid lipid nanoparticles (SLNs). The developed method was used for detection of shelf life of haloperidol in SLNs. Calibration curve of haloperidol was also constructed in rat plasma using loratidine as internal standard. In vivo studies were performed on rats and concentration of haloperidol in brain and blood was measured for the determination of various pharmacokinetic and hence brain targeting parameters. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate-acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haloperidol/quantification; Haloperidol/pharmacokinetic; Haloperidol/in vitro study; Haloperidol/in vivo study; High performance liquid chromatography/solid lipid nanoparticles; Plasma; Validation; Brain targeting..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200623
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Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination BJPS
Razzaq,Syed Naeem; Ashfaq,Muhammad; Khan,Islam Ullah; Mariam,Irfana; Razzaq,Syed Saleem; Mustafa,Ghulam; Zubair,Muhammad.
Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gatifloxacin; Dexamethasone; High performance liquid chromatography; Validation; Plasma; Degradation products..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100607
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Determination of Gemifloxacin in human plasma by high performance liquid chromatography using Ultra Violet detector and its application to a bioequivalence study BJPS
Mousavi,Syed Husain Hashemi.
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610
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Effects of diets with increasing levels of citrus pulp on the blood parameters linked to energy metabolism in horses Ciência e Agrotecnologia
Menezes,Madalena Lima; Brandi,Roberta Ariboni; Bueno,Ives Cláudio da Silva; Balieiro,Júlio Cesar de Carvalho; Moreira,Camilla Garcia; Nascimento,Olivia Carmen de Araújo.
Currently, the use of alternative energy ingredients for horses has increased because these foods contain "superfibers", making safer diet for these animals. To study the influence of diets containing increasing levels of citrus pulp on albumin, triglycerides, cholesterol, glucose, insulin and short chain fatty acid (SCFA) concentrations in the blood, five animals were used, with an average age of 3.5 years and a live weight of 460.66±76.86 kg, they were fed twice a day, at 7:00 am and 4:00 pm. The diets were formulated to meet the requirements of the animals at maintenance. 60% of the energy was obtained from forage, and 40% from concentrate, containing the following inclusion levels of citrus pulp (0, 7, 14, 21 and 28%). The area under the curve (AUC)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soluble fiber; Nutrition; Pectin; Plasma.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000600008
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Phytase in rations of growing pigs: performance, blood parameters and bone mineral content Ciência e Agrotecnologia
Silva,Hunaldo Oliveira; Fialho,Elias Tadeu; Freitas,Rilke Tadeu Fonseca de; Lima,José Augusto de Freitas; Logato,Priscila Rosa Vieira; Schoulten,Neudi Artemio.
It was intended with the present work to verify the effect of phytase on the performance, bone mineralization and content of plasma urea of swine. 80 swine (30 ± 3.1 kg/LW), crossbred (LD x LW) males and females, allocated to a randomized block design (RBD) with four treatments and five replicates. The treatments consisted of a growing swine diet on the basis of corn, soybean meal and defatted rice bran (DRB) supplemented with four levels of phytase (0, 400, 800 and 1200 FTU/kg) The diet was formulated to meet the requirements of growing swine except for available calcium and phosphorus, the levels of which were 0.64 and 0.25%, respectively. The enzyme phytase utilized was Natuphos 5000. Over the experimental period every eight days, blood of two animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme; Minerals; Plasma; Urea.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600028
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Eletroforese das proteínas plasmáticas em emas (Rhea americana) de diferentes faixas etárias Ciência Rural
Conrado,Alexandre de Carvalho; Lopes,Sonia Terezinha dos Anjos; Martins,Danieli Brolo; Duarte,Marta Frescura; Mortari,Antonio Carlos; Flores,Maristela Lovato; Barasuól,Luciane.
A rheacultura vem crescendo em todo o Brasil. Porém, ainda há uma escassez de informações sobre esta espécie e de médicos veterinários especializados na área. Este trabalho teve como objetivo verificar as diferenças existentes na eletroforese plasmática (gel de agarose) em idades distintas de emas (Rhea americana). Utilizaram-se 45 emas, separadas em quatro grupos: grupo 1 (n=10), animais com 15 dias de idade; grupo 2 (n=10), animais com 30 dias; grupo 3 (n=10), animais com 45 dias e grupo 4 (n=15), animais com 1 ano. Verificou-se homogeneidade nos valores eletroforéticos analisados nas aves dentro de cada faixa etária. Houve diferença entre grupos etários em valores de proteína plasmática total, albumina, globulinas e relação albumina/globulinas. Também...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eletroforese; Plasma; Rhea americana; Idade.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400018
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Utilização de plasma frio no aumento da molhabilidade de produtos à base de madeira. Infoteca-e
CADEMARTORI, P. H. G. de; SOUZA JUNIOR, L. R. de; MUNIZ, G. I. B. de; MAGALHAES, W. L. E..
Tipo: Folhetos Palavras-chave: Madeira; Plasma.
Ano: 2016 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1052592
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AN IN VITRO STUDY OF THE EFFECTS OF VENOM OF AUSTRALIAN ELAPIDS ON MURINE SKELETAL MUSCLE AND THE PROTECTIVE EFFECT OF HOMOLOGOUS PLASMA J. Venom. Anim. Toxins
BUTLER,T. L.; JACOBSEN,P. F.; MIRTSCHIN,P. J.; KAKULAS,B. A..
The venom of many dangerous Australian snakes has a myotoxic component and some are strongly myolytic. The myotoxicity of venom of seven Australian elapid snakes was studied to determine their relative in vitro potency in causing cell death of C2C12 cells, a myoblast cell line, and murine myotubes in mixed cell culture. The venom of Pseudechis australis proved to be the most myotoxic, Austrelaps superbus and Pseudechis porphyriacus venoms also exhibited high myotoxicity relative to the other venoms tested. The specificity of Pseudechis porphyriacus venom was tested using the human glioma cell line TC3 and was shown to exhibit a general cytotoxicity. Myotoxicity, however, was the predominant action of the venom. It has long been known that certain animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom antidote; Plasma; Myotoxicity; Murine skeletal muscle; Australian elapids.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100004
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Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy &amp; Gaimard, 1824 and) Leporinus obtusidens (Valenciennes, 1837) Neotropical Ichthyology
Loro,Vania Lucia; Glusczak,Lissandra; Moraes,Bibiana S.; Leal,Claudio A. M.; Menezes,Charlene; Murussi,Camila R.; Leitemperger,Jossiele; Schetinger,Maria Rosa C.; Morsch,Vera Maria.
Rhamdia quelen (silver catfish) and Leporinus obtusidens (piava) were exposed to a commercial formulation Roundup(r), a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase) activities. Plasmatic glucose levels were reduced in both species, whereas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Mucus; Nucleotidases; Plasma; Protein carbonyl.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100229
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Changes of Progesterone and Endothelin Concentrations in the Peripheral Plasma of Female Calves and Cycling Cows: Effects of PGF2α Injection OAK
OHTANI, Masayuki; OKI, Naoko; TANIMURA, Miyune; KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; MIYAMOTO, Akio; 宮本, 明夫.
It is well known that an injection of prostaglandin F2α (PGF2α) or its analogue during the mid luteal phase of the estrous cycle induces a rapid decrease in plasma progesterone (P4) concentration, followed by luteolysis in the cow. There is evidence that a potent vasoactive peptide, endothelin-1 (ET-1), is produced in the bovine corpus luteum (CL), and that it is directly involved in luteolysis. We previously found that ET-1 concentrations in the peripheral plasma increase during the period of luteolysis and ovulation in cows. However, it is not clear whether the elevation of peripheral plasma ET-1 concentration observed during luteolysis and ovulation originates exclusively from the ovary and/or CL. Such a profile of plasma ET-1 concentration may be...
Palavras-chave: Endothelin; Progesterone; Plasma; Calves; Cows.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2973
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