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| Registros recuperados: 44 | |
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| LeBlanc-Straceski,Janine; Sobrado,Pablo; Betz,Sharon; Zerfas,Julie; Morgan,Karen. |
| A PCR based strategy was developed to screen a Xenopus oocyte λgt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, which are used as template for PCR. The integrity of the plaques on the low-density plates is preserved. Once a positive LP has been identified,... |
| Tipo: Journal article |
Palavras-chave: CDNA; Lambda; Non-radioactive; PCR; Plaque; Screening. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012 |
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| Inoue-Nagata,Alice K; Nagata,Tatsuya; Ávila,Antonio C de; Giordano,Leonardo de B. |
| Due to failures of infection after mechanical inoculation of begomovirus on tomato (Lycopersicon esculentum Mill.) plants, an efficient and reliable begomovirus inoculation method was developed using the whitefly vector (Bemisia tabaci). Virus acquisition was carried out using a 'Tube-cage', made of a polypropylene tube, containing one to two day-old whiteflies and a tomato plant apex infected with a begomovirus isolate. After 48 h of acquisition access period, inoculation was done using a 'Ring-cage', containing viruliferous whiteflies, attached to the abaxial side of the leaf of young tomato plants. 48 hours after acquisition, the whiteflies were eliminated and the inoculated plants were incubated for 28 days under greenhouse conditions. Three... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Lycopersicon esculentum; Inoculation; Resistance; Screening; Geminivirus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000300024 |
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| Silva,Alysson J da; Oliveira,Gustavo HF de; Pastoriza,Rhuan JG; Maranhão,Eduardo HA; Pedrosa,Elvira MR; Maranhão,Sandra RVL; Boiteux,Leonardo S; Pinheiro,Jadir B; Carvalho Filho,José Luiz S de. |
| ABSTRACT Meloidogyne enterolobii (=M. mayaguensis) is an emerging plant pathogen capable of inducing root galls and yield reduction in a wide range of host species. This pathogen has also been reported as a global threat for tomato (Solanum lycopersicum) crop production mainly due to its ability to overcome the resistance meditated by the Mi-1 gene. Despite the potential importance of this nematode, sources of resistance to M. enterolobii are not yet available for breeding purposes. The main objective of the present work was to evaluate a large Solanum (section Lycopersicum) germplasm (comprising nine species and one botanic variety) aiming to identify useful sources of resistance to M. enterolobii. In the first screening assay, 101 accessions and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum spp.; Germplasm; Screening; Root-knot nematode. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200188 |
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| Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida. |
| Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic... |
| Tipo: Text |
Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf |
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| Lalegerie, Fanny; Lajili, Sirine; Bedoux, Gilles; Taupin, Laure; Stiger-pouvreau, Valerie; Connan, Solène. |
| To cope with the biotic and abiotic stresses experienced within their environment, marine macroalgae have developed certain defence mechanisms including the synthesis of photo-protective molecules against light and particularly harmful UV radiation. The aim of this study was to screen selected red algae, a highly diverse phylogenetic group, for the production of photo-protective molecules. The pigment content and composition (i.e. chlorophyll-a, phycobiliproteins and carotenoids) and the composition of mycosporine-like amino acids (MAAs) were studied in 40 species of red macroalgae collected in Brittany (France), at two distinct periods (i.e. February and July 2017). A high inter-specific variability was demonstrated in terms of pigment content and MAA... |
| Tipo: Text |
Palavras-chave: Algae; Diversity; HPLC; MAAs; Metabolites; Photo-protection; Pigments; Rhodophyta; Screening. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00488/59929/63133.pdf |
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| Molinari, Nicolas; Abou-badra, Mathieu; Marin, Gregory; Ky, Chin-long; Amador, Noemi; Gamez, Anne Sophie; Vachier, Isabelle; Bourdin, Arnaud. |
| Background: COPD is largely under-diagnosed and once diagnosed usually at a late stage. Early diagnosis is thoroughly recommended but most attempts failed as the disease is marginally known and screening marginally accepted. It is a rare cause of concern in primary care and spirometry is not very common. Exhaled carbon monoxide (eCO) is a 5-seconds easy-to-use device dedicated to monitor cigarette smoke consumption. We aimed to assess whether systematic eCO measurement in primary care is a useful tool to improve acceptance for early COPD diagnosis. Methods: This was a two-center randomized controlled trial enrolling 410 patients between March and May, 2013. Whatever was the reason of attendance to the clinic, all adults were proposed to measure eCO during... |
| Tipo: Text |
Palavras-chave: COPD; Screening; Exhaled CO; Smoking; Behaviour. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38142/36280.pdf |
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| Wiernasz, Norman; Cornet, Josiane; Cardinal, Mireille; Pilet, Marie-france; Passerini, Delphine; Leroi, Francoise. |
| As fragile food commodities, microbial and organoleptic qualities of fishery and seafood can quickly deteriorate. In this context, microbial quality and security improvement during the whole food processing chain (from catch to plate), using hurdle technology, a combination of mild preserving technologies such as biopreservation, modified atmosphere packaging and superchilling, are of great interest. Thirty-five lactic acid bacteria (LAB) belonging to the species Carnobacterium maltaromaticum, Carnobacterium inhibens, Lactococcus piscium, Leuconostoc gelidum, Vagococcus fluvialis, Vagococcus penaei and Aerococcus viridans, known to possess interesting antimicrobial activity, were chosen for their potential application as bioprotective agents as a part of... |
| Tipo: Text |
Palavras-chave: Antimicrobial activity; Spoilage; Screening; Chitosan; Fish juice; Safety assessment. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00382/49342/49732.pdf |
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| Dufourcq, R.; Chalkiadakis, Eletherios; Fauchon, M.; Deslandes, Eric; Kerjean, V.; Chanteau, S.; Petit, Elodie; Guezennec, Jean; Dupont-rouzeyrol, M.. |
| Marine bacteria are a rich source of bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of New Caledonia. We obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). Ten out of the most promising strains were cultured, fractionated and screened for antibacterial activity. Four of them (NC282, NC412, NC272 and NC120) showed at least an activity against reference and... |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Characterization; Marine bacteria; New Caledonia; Screening. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00174/28552/26994.pdf |
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| 相内, 大吾; 馬場, ゆき子; 稲見, 圭吾; 新屋, 良治; 谷, 昌幸; 倉持, 勝久; 堀江, 早弥佳; 小池, 正徳. |
| In a previous study, protoplast fusion was performed among 3 strains of the entomopathogenic fungi Verticillium lecanii, as a result of which 174 hybrid strains were obtained. The aim of the present study was to screen desirable hybrid strains that have a wide host range or durable effects. Initially, 43 hybrid strains were used in bioassays against the cotton aphid. Of these strains, 30 induced equal or higher mortality as compared to Vertalec (41.9%). Secondly, 50 hybrid strains were used in bioassays against the greenhouse whitefly. Of these strains, 37 exhibited an equal or higher infection rate as compared to that of Mycotal (36.2%). Finally, 50 hybrid strains were applied to cucumber leaves in order to test strain viability under low humidity... |
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Palavras-chave: Biological control; Lecanicillium spp; Protoplast fusion; Screening; Verticillium lecanii. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2587 |
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| Hendrikse, George W.J.; Hu, Yamei. |
| Various studies show that agricultural cooperatives behave differently than their investor-owned counterparts. One explanation may be that the internal decision making process differs in these two governance structures. A model is developed to explore how endogenous screening rules affect efficient organizational choices and industrial structures. It is shown that screening level choice may outweigh architecture choice and that screening rules are strategic substitutes. Conditions are derived under which cooperatives are efficient organizational forms. It is also shown that competition may increase the attractiveness of investor-owned firms and circumstances are determined in which cooperatives and investor owned firms coexist in equilibrium. |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Architecture; Screening; Cooperatives; Duopoly; Agribusiness; Q13. |
| Ano: 2005 |
URL: http://purl.umn.edu/24522 |
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| Bourzama,G; Ouled-Haddar,H; Marrouche,M; Aliouat,A. |
| ABSTRACT The Metal pollution is one of the major risks in the world nowadays. Iron is an essential metal for growth and proliferation of a vast majority of organisms, but it can be toxic to human health and other living beings in the environment at high concentrations due to its increased industrial activity. Fungi have a remarkable capacity to uptake and detoxify iron metal using different mechanisms such as bioaccumulation. Thus, the aim of this work is to study the ability of iron uptake by the fungal strains isolated from Arcelor Mittal -Annaba- in Algeria. Three strains were screened at high concentration of iron (1 g/l) and their capacity to uptake iron has been studied on Czapek Yeast Agar medium. The amounts of uptaken iron ions were estimated in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Iron; Screening; Toxic; Uptake. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100313 |
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| Duenpen Wongsorn; Siviali Sirimungkararat; Weerasak Saksirirat. |
| The selection of eri silkworm ecorace with high yield and distinct in morphological character is necessary for variety improvement. Five eri silkworm ecoraces, SaKKU1, SaKKU2, SaKKU3, SaKKU4 and SaKKU5 were surveyed and derived in country or from academic cooperation in international level. All ecoraces were cultured using castor leaves of TCO101 variety as food plant at 25±2 °C, 80±5%R.H. Based on morphological characters, there were similar except body of the 5th instar larva of SaKKU1 was covered with white powder and ripe larva showing shiny dominant yellow color. Life cycles were also similar durations, 46-53 days (SaKKU1), 42-53 days (SaKKU2), 42-52 days (SaKKU3), 40-56 days (SaKKU4) and 41-52 days (SaKKU5). SaKKU1 had highest survival rate at larval... |
| Tipo: PhysicalObject |
Palavras-chave: Ecorace eri; Samiaricini; Silkworm; Variety improvement; Screening; Morphological; Growth; Yields; ไหมอีรี่; แมลง; การปรับปรุงพันธุ์; การคัดเลือก; ลักษณะทางสัณฐานวิทยา; การเจริญเติบโต; ความแข็งแรง; ผลผลิต; การอยู่รอด. |
| Ano: 2013 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5771 |
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| Registros recuperados: 44 | |
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