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| Registros recuperados: 63 | |
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| SAKATE,M.; OLIVEIRA,P. C. LUCAS DE. |
| Toads (order: Anura; family: Bufonidae; genus: Bufo) are distributed throughout the world, but more species are found in areas of tropical and humid temperate climates. Although toads do not have a venom inoculation system, they are venomous animals because the glands covering the whole surface of their bodies secrete a milk-like venom of which composition is not yet completely known. Some of these glands are the bilateral glands located in post-orbital position. These glands, which are somewhat diamond-shaped and can be seen by the naked eye, are known as parotids. Toad envenoming in dogs may cause local and systemic alterations and may cause death by cardiac ventricular fibrillation. The electrocardiographic alterations observed consist of gradual... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toad; Bufo; Venom; Dogs; Envenoming. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000100003 |
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| LIMA,P. R. M. DE; BROCHETTO-BRAGA,M. R.; CHAUD-NETTO,J.. |
| Some properties of a Africanized honeybee venom proteases were determined by enzymatic assays in solution, electrophoresis in SDS-PAGE, and gel filtration. Bee venom extracts were obtained by reservoir disruption, selective dialysis (cut off 12 kDa) to eliminate small components, such as the protease inhibitor present in the venom, and then fractionation of the dialyzed extract by gel filtration on a Sephadex G-100 column. The optimal conditions for the caseinolytic assays were pH 9.5, 2-hour digestion at 37 °C, and 1% casein concentration. The proteolytic activity was also determined by electrophoresis in SDS-PAGE with co-polymerized gelatin with three major bands of 66.0, 41.6, and 25.1 kDa. A principal serine-protease-like mechanism was revealed in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venom; Africanized bee; Apis mellifera; Proteolytic activity; Gel filtration chromatography. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000100004 |
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| SILVA,R. J. DA; FECCHIO,D.; BARRAVIERA,B.. |
| The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10<img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"> tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1<img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"> , 4<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 7<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 10<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venom; Inflammation; Tumor growth; Crotalus durissus terrificus. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008 |
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| Segura-Puertas,L.; Avila-Soria,G.; Sánchez-Rodríguez,J.; Ramos-Aguilar,M. E.; Burnett,J. W.. |
| Aurelia aurita is a scyphozoan, abundant in the Mexican Caribbean during summer. Although usually innocuous, there is evidence of it causing harm to humans. This work investigates the biological activities of crude and fractionated extracts of A. aurita. Live specimens were collected between July and September 1999 from the Mexican Caribbean. The tentacular margin was dissected immediately and frozen at -50ºC. A nematocyst suspension was prepared, discharged, and the supernatants lyophilized. Hemolytic assay was performed with lyophilized crude extract on bovine, sheep, and human red blood cells. Erythrocyte sensitivity to the toxin was ranked in descending order: human, sheep, and bovine. Toxic activity on Artemia nauplii was evaluated using the same... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aurelia aurita; Sting; Hemolytic activity; Crustacean lethality; Venom. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200007 |
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| BARRAVIERA,B.; SARTORI,A.; PEREIRA DA SILVA,M. F.; KANENO,R.; PERAÇOLI,M. T. S.. |
| A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme-linked immunosorbent assay; Crotalus durissus terrificus; Venom; Antivenom; Antibodies; Cerebrospinal fluid. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100003 |
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| Ribeiro,Mara Fernandes; Oliveira,Felipe Leite de; Souza,Aline Moreira; Machado,Thelma de Barros; Cardoso,Priscilla Farinhas; Patti Sobrinho,Andrea; Nascimento,Angélica Silveira; Souza,Cláudio Maurício Vieira de; Elias,Sabrina Calil. |
| ABSTRACT Background: Accidents caused by spiders of the genus Loxosceles constitute an important public health problem in Brazil. The venom of Loxosceles sp induces dermonecrosis at the bite site and systemic disease in severe cases. Traditional medicine based on plant-derived products has been proven to reduce the local effects of envenomation. The present study verified the healing effects of copaiba oil on lesions induced by the venom of L. intermedia. Methods: Cutaneous lesions were induced on the backs of rabbits by intradermal injection of L. intermedia venom. Copaiba oil was applied topically 6 hours after injection; the treatment was repeated for 30 days, after which animal skins were removed and processed for histopathological analysis. Blood... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loxosceles intermedia; Venom; Skin lesion; Copaiba oil; Topical treatment. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100310 |
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| Candido,D. M.; Lucas,S.. |
| This work presents the experience of the Laboratory of Arthropods at Instituto Butantan, which maintains scorpions in captivity in order to obtain the venom used in the production of anti-arachnid serum. Between 1993 and 2000, the laboratory received 24.781 specimens of Tityus serrulatus in order to obtain poison for the production of anti-scorpion serum. In the first extraction, performed by electrical stimulation, the animals gave an average quantity of 0.4 mg venom per specimen. Animal acquisition and involved professional safety are considered. In addition, the captivity, handling and feeding techniques are described, as well as the method and equipment used for venom extraction. It shows the importance of regular campaigns, offering information to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpions; Tityus; Maintenance; Venom. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100007 |
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| Jalali,A; Pipelzadeh,M H; Seyedian,R; Rahmani,A H; Omidian,N. |
| The available Razi Institute antivenom is still, empirically, used by intramuscular (IM) administration for the treatment of scorpion stings in humans by six medically dangerous species including Hemiscorpius lepturus (H. lepturus). The aim of this study was to assess the neutralizing ability and effectiveness of the antivenom in inhibiting hemoglobinuria, biochemical changes, increased microalbuminuria and urinary lactate dehydrogenase (LDH) following H. lepturus sting. Simultaneous intramuscular administration of 10 μL and 100 μL of antivenom, after 24 hours, had no significant preventive effect on the extent and degree of hemoglobinuria or proteinuria produced in venom-treated rats. After IM administration of antivenom, no significant changes in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemiscorpius lepturus; Scorpion; Venom; Hemolysis; Antivenom. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200004 |
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| Troiano,J. C.; Gould,E. G.; Gould,I.. |
| A comparative study on the sensitivity of erythrocytes from different vertebrate species (avian, mammalian and reptilian) to the hemolytic action caused by cardiotoxin isolated from Naja naja atra venom was carried out. Cardiotoxin was able to induce direct hemolysis in washed erythrocytes from several animals, except for llama. The EC50 values from hemolysis of the most sensitive (cat) and the most resistant (snake) animal varied approximately tenfold. According to the cell behavior, it was possible to characterize four types of behavior: The first was observed in cat, horse and human cells; the second in rat, rabbit and dog erythrocytes; and the third only in llama erythrocytes, which were resistant to cardiotoxin concentrations up to 300 µg/ml. Finally,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemolysis; Cobra; Venom; Erythrocytes; Animals. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100004 |
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| Tang,MS; Vejayan,J; Ibrahim,H. |
| Two-dimensional gel electrophoresis (2DE) is an important tool for investigating the complexity of snake venom proteomes. Apart from applications based on whole proteome analysis, we suggest that 2DE can be used as an assay to guide the progress of protein purification. The aim of this study was to prove the feasibility of this concept by using it to purify rhodocetin from Calloselasma rhodostoma venom. Rhodocetin (α subunit) spot on the 2DE profile of C. rhodostoma venom was first identified and confirmed by mass spectrometry, with a molecular mass of 16 kDa and calculated pI of 5.16. Rhodocetin was subsequently purified by successive anion-exchange and gel filtration chromatography. Every peak from both chromatography profiles was collected and tested on... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein fingerprinting; Liquid chromatography; Proteomics; Venom. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400011 |
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| Ramírez-Avila,J.; Quevedo,B. E.; López,E.; Renjifo,J. M.. |
| Components with phospholipase A2 activity were isolated by gel filtration and cationic exchange chromatography from the venom of Bothrops asper snakes from Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE). Fractions II and VII exhibited an optimal activity at pH 9 and 52ºC. The optimum calcium concentration for fraction II was 48 mM and for fraction VII, 384 mM. Both fractions showed thermal stability. Fraction II was stable at pH values between 2.5 and 9, and fraction VII, between 2.5... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phospholipase; Isoenzymes; Snake; Venom; Bothrops asper; Colombia. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300005 |
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| Registros recuperados: 63 | |
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