|
|
|
| Registros recuperados: 2.463 | |
|
|
| Dantas,Liliane G.; Guerra,Marcelo. |
| A comparative analysis of mitotic chromosomes of Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) was performed aiming to identify cytological differences between the two most important species of this genus. Both species have symmetric karyotypes, with 2n = 20 metacentric chromosomes ranging in size from 2.00 to 1.19 µm (cacao) and from 2.21 to 1.15 mm (cupuaçu). The interphase nuclei of both species were of the arreticulate type, displaying up to 20 chromocentres, which were more regularly shaped in cacao than in cupuaçu. Prophase chromosomes of both species were more condensed in the proximal region, sometimes including the whole short arm. Both species exhibited only one pair of terminal heterochromatic bands, positively stained with chromomycin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cacao; Cupuaçu; Heterochromatin; Karyotype; RDNA sites; Theobroma. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100017 |
| |
|
|
| Campos,Carlos F.; Pereira,Boscolli B.; Campos-Junior,Edimar O. de; Sousa,Eduardo F.; Souto,Henrique N.; Morelli,Sandra. |
| Abstract Pollutants have adverse effects on human health and on other organisms that inhabit or use water resources. The aim of the present study was to assess the environmental quality of three watercourses in Monte Carmelo, MG, Brazil, using the micronucleus test on Tradescantia. For each treatment, 15 plants were exposed to water samples for 24 h. The control group was exposed to formaldehyde (0.2%) and the negative control to Hoagland solution. Subsequently the plants were placed in Hoagland solution for 24 h to recover. Cells were stained with 2% acetic carmine and examined by light microscopy. Three hundred tetrads were analyzed per slide. The frequency of genotoxic alterations was expressed as the number of micronuclei per 100 tetrads, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomonitoring; Genotoxicity; Micronucleus; Tradescantia pallida. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400507 |
| |
|
|
| Maia,Ivan G.; Leite,Adilson. |
| The N-linked glycosylation of secretory and membrane proteins is the most complex posttranslational modification known to occur in eukaryotic cells. It has been shown to play critical roles in modulating protein function. Although this important biological process has been extensively studied in mammals, much less is known about this biosynthetic pathway in plants. The enzymes involved in plant N-glycan biosynthesis and processing are still not well defined and the mechanism of their genetic regulation is almost completely unknown. In this paper we describe our first attempt to understand the N-linked glycosylation mechanism in a plant species by using the data generated by the Sugarcane Expressed Sequence Tag (SUCEST) project. The SUCEST database was... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100030 |
| |
|
|
| Diniz-Filho,José Alexandre F.; Santos,Thiago; Rangel,Thiago Fernando; Bini,Luis Mauricio. |
| Several metrics have been developed for estimating phylogenetic signal in comparative data. These may be important both in guiding future studies on correlated evolution and for inferring broad-scale evolutionary and ecological processes (e.g., phylogenetic niche conservatism). Notwithstanding, the validity of some of these metrics is under debate, especially after the development of more sophisticated model-based approaches that estimate departure from particular evolutionary models (i.e., Brownian motion). Here, two of these model-based metrics (Blomberg's K-statistics and Pagel's λ) are compared with three statistical approaches [Moran's I autocorrelation coefficient, coefficients of determination from the autoregressive method (ARM), and phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autocorrelation; Blomberg's K; Pagel's lambda; Autoregressive method; Moran's I; Phylogenetic eigenvector regression. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400019 |
| |
|
| |
|
|
| Tomazett,Mariana Vieira; Zanoelo,Fabiana Fonseca; Bailão,Elisa Flávia Cardoso; Bailão,Alexandre Melo; Borges,Clayton Luiz; Soares,Célia Maria de Almeida. |
| Abstract Carbonic anhydrases (CA) belong to the family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the present work, we characterized the cDNAs of four Paracoccidioides CAs (CA1, CA2, CA3, and CA4). In the presence of CO2, there was not a significant increase in fungal ca1, ca2 and ca4 gene expression. The ca1 transcript was induced during the mycelium-to-yeast transition, while ca2 and ca4 gene expression was much higher in yeast cells, when compared to mycelium and mycelium-to-yeast transition. The ca1 transcript was induced in yeast cells recovered directly from liver and spleen of infected mice, while transcripts for ca2 and ca4 were down-regulated. Recombinant CA1 (rCA1) and CA4 (rCA4), with 33... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carbonic anhydrases; Paracoccidioides; CO2; Gene expression; Enzyme assay. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300416 |
| |
|
|
| Nassar,Nagib M.A.. |
| Wild species of Manihot are progenitors of cassava. They constitute valuable genetic reservoirs presenting genes that show new characters. Screening of these species showed some of them to have a notably high percentage of protein combined with a low percentage of hydrocyanic acid. Study of natural habitats revealed resistance to drought and excessive soil aluminum toxicity as well as adaptation to low temperature. Some of the hybrids obtained showed high root productivity and resistance to stem borers. Apomixis was discovered in the wild and transferred successfully to the cultivate species. |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100035 |
| |
|
| |
|
|
| Belanova,Anna A.; Smirnov,Dmitry S.; Makarenko,Maxim S.; Belousova,Mariya M.; Mashkina,Elena V.; Aleksandrova,Anzhela A.; Soldatov,Alexander V.; Zolotukhin,Peter V.. |
| Abstract Pathway activity assessment-based approaches are becoming highly influential in various fields of biology and medicine. However, these approaches mostly rely on analysis of mRNA expression, and total mRNA from a given locus is measured in the majority of cases. Notably, a significant portion of protein-coding genes produces more than one transcript. This biological fact is responsible for significant noise when changes in total mRNA transcription of a single gene are analyzed. The NFE2L2/AP-1 pathway is an attractive target for biomedical applications. To date, there is a lack of data regarding the agreement in expression of even classical target genes of this pathway. In the present paper we analyzed whether transcript variants of GPX2, NQO1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NFE2L2/AP-1 pathway; Interactomics; Transcript variants expression control. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300515 |
| |
|
|
| Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui. |
| This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013 |
| |
|
|
| Santos,Marcelo dos; Schwantes,Maria Luiza B.; Schwantes,Arno Rudi. |
| The scale number in lateral sets (SNS) of Mugil sp. (Mugilidae, Perciformes) collected in the lagoon-estuarine region of Cananéia, State of São Paulo ranges from 33 to 39. Electrokinetic, kinetic and thermostability properties of lactate dehydrogenase (LDH) were tested to determine if individuals with different SNS correspond to different species or populations of mullet. As in many other teleosts, LDH-A*, LDH-B*, and LDH-C* loci were detected. Through a two-fold serial dilution method applied to 10 different tissues of Mugil sp., a bidirectionally divergent expression of these loci was suggested. No association among LDH electrophoretic pattern, thermal inactivation, kinetic responses and different SNS was observed. The apparent Km (pyr) values obtained... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100026 |
| |
|
|
| Hou,Zhaoqi; Jia,Bing; Li,Fei; Liu,Pu; Liu,Li; Ye,Zhenfeng; Zhu,Liwu; Wang,Qi; Heng,Wei. |
| Abstract The plant genes encoding ABCGs that have been identified to date play a role in suberin formation in response to abiotic and biotic stress. In the present study, 80 ABCG genes were identified in ‘Dangshansuli’ Chinese white pear and designated as PbABCGs. Based on the structural characteristics and phylogenetic analysis, the PbABCG family genes could be classified into seven main groups: classes A-G. Segmental and dispersed duplications were the primary forces underlying the PbABCG gene family expansion in ‘Dangshansuli’ pear. Most of the PbABCG duplicated gene pairs date to the recent whole-genome duplication that occurred 30~45 million years ago. Purifying selection has also played a critical role in the evolution of the ABCG genes. Ten PbABCG... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pear; Russet mutant; ABCG; Evolution; Gene expression. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100137 |
| |
|
|
| Montalván,Ricardo; Ando,Akihiko. |
| Seeds of rice cultivar IAC-1246 received single and combined treatments of 10 or 20 Krad gamma-rays and 0.5 mM sodium azide (SA). The experiments were carried out to assess the effect of treatments on the mean and variance in second generation plants of the following quantitative traits: number of days to flowering (NDF), culm length (CL) and tiller number (TN). In general, the mutagenic treatments increased variance, but did not change the mean for the characters NDF and CL in the M2 generation. There was no increase in the mean or variance of TN. The combined treatments of gamma-rays and SA resulted in larger variance in CL than for the treatments with gamma-rays alone, but not higher than obtained with SA alone. Combined treatments with gamma-rays and... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100014 |
| |
|
|
| Cheng,Yuanzhi; Wang,Rixin; Sun,Yuena; Xu,Tianjun. |
| To understand the phylogenetic position of Larimichthys polyactis within the family Sciaenidae and the phylogeny of this family, the organization of the mitochondrial genome of small yellow croaker was determined herein. The complete, 16,470 bp long, mitochondrial genome contains 37 mitochondrial genes (13 protein-coding, 2 ribosomal RNA and 22 transfer RNA genes), as well as a control region (CR), as in other bony fishes. Comparative analysis of initiation/termination codon usage in mitochondrial protein-coding genes of Percoidei species, indicated that COI in Sciaenidae entails an ATG/AGA codon usage different from other Percoidei fishes, where absence of a typical conserved domain or motif in the control regions is common. Partitioned Bayesian analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Larimichthys polyactis; Mitochondrial genome; Control region; Partitioned Bayesian analysis; Sciaenidae. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100027 |
| |
|
|
| Pereira Júnior,Hélio Rubens Jacintho; Jorge,Wilham; Costa,Maria Elvira Loyola Teixeira da. |
| Anteaters belong to the Order Xenarthra / Family Myrmecophagidae and are the only members without teeth. There are three genera with four living species in the family Myrmecophagidae: Myrmecophaga tridactyla (giant anteater), Tamandua tetradactyla (southern lesser anteater), Tamandua mexicana (northern lesser anteater), and Cyclopes didactylus (silky anteater). The karyotypes of M. tridactyla (2n = 60), T. tetradactyla (2n = 54) and C. didactylus (2n = 64) have already been described. In the present paper, three female and two male specimens of giant anteater and one lesser anteater male were analyzed. The results indicate the existence of a new karyotype in the genus Tamandua, with 2n = 56 chromosomes, which can represent a new lesser anteater species.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anteater; Chromosomes; Myrmecophagidae. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300014 |
| |
|
|
| Gimenes,Marcos Aparecido; Lopes,Catalina Romero. |
| There are more than 200 races of maize (Zea mays L.) divided into three groups (ancient commercial races, the recent commercial races, and indigenous races). Although the indigenous races have no commercial value, they have many important characteristics which can be incorporated into maize breeding programs. Most Brazilian indigenous germplasm race stocks were collected at least 40 years ago, and nothing is known of the genetic variability present in this germplasm. The genetic variability was assayed in 15 populations from four indigenous races of maize (Caingang, Entrelaçado, Lenha and Moroti) and five indigenous cultivars, using five isoenzymatic systems encoded by 14 loci. The analysis revealed a low level of variability among the samples studied.... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200023 |
| |
|
|
| Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda. |
| Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brain tumors; Epigenetics; Methylation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001 |
| |
|
| |
|
| |
|
|
| Iorio,Mario; Vincenti,Donatella; Annunziata,Mario; Rullo,Rosario; Bonamassa,Raffaele; Di Luccia,Aldo; Pieragostini,Elisa. |
| On 398 river buffalo samples, randomly collected in distinct breeding areas of the Campania region, high-resolution analytical systems were used to identify both qualitative and quantitative variations of the Hb phenotype. Polyacrylamide gel isoelectric focusing and HPLC were used to determine the ratio between HBA1 and HBA2 globin chains; restriction endonuclease analysis was performed to assess whether quantitative variations in Hb bands were related to an unusual number of a-globin genes. In the two buffalo subpopulations, allele frequencies of the alpha and beta globin systems were calculated, and F statistics (FIS, FIT and FST) were estimated as parameters of genetic diversity. The results suggest that: i) as shown by RFLP analysis, only a couple of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenotype; RFLP; Globin genes; Haplotype; Gene frequencies. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200007 |
| |
| Registros recuperados: 2.463 | |
|
|
|
