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| Registros recuperados: 225 | |
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| Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle. |
| The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses.... |
| Tipo: Text |
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf |
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| Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l1. Parasites were collected after 12, 24 and 48 h of... |
| Tipo: Text |
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf |
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| Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno. |
| Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés. |
| Tipo: Text |
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf |
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| Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.. |
| The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,... |
| Tipo: Text |
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf |
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| Registros recuperados: 225 | |
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