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| Registros recuperados: 4.144 | |
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| Hashimoto, Makoto; Nabeta, Kensuke; Murakami, Kentaro. |
| 3-Trifluoromethylphenyldiazirine based oleic acids derivatives are synthesized to elucidate the functions of specific activation of protein kinase C (PKC) with oleic acid. The synthetic route is based on the alkylation of phenolic derivative with oleic acid equivalent and the post-functionalization. of the compound to achieve radiolabeling. Several compounds have biological activity for PKC with similar efficacy with that of oleic acid. The results indicated that the diaizinyl oleic acid derivatives should be useful to study the specific functions of oleic acid for PKC. (C) 2003 Elsevier Science Ltd. All rights reserved. |
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Palavras-chave: PHENYLALANINE; DIAZIRINE; RESPONSES; ANALOGS; PROBES. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1324 |
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| Khukhuu, A; Sivakumar, T; Alhassan, A; Ota, N; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男. |
| Recently, Babesia bovis genome project identified a novel gene, which is homologous to the gene encoding sporozoite P36 antigen in Plasmodium parasites. In the present study, we isolated the P36 homologous gene from the genomic DNA of B. bovis and designated it as the BvP36 gene. The sequence of the BvP36 gene was identical to that reported previously. This gene was expressed in an Escherichia coli system to produce a recombinant protein (rBvP36). The rBvP36 protein was produced as a highly soluble form in E. coli and was detected by western blot analyses of sera collected from cattle experimentally infected with B. bovis and B. bigemina merozoites. In indirect immunofluorescence tests, sera collected from mice immunized with rBvP36 showed strong reactions... |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2385 |
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| 高橋, 裕司; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKAHASHI, Yuji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
| CM-Toyopearl陽イオン交換カラムクロマトグラフィーおよびSephacryl S-200ゲルろ過カラ ムクロマトグラフィーによって、常温性菌核病菌Sclerotinia sclerotiorumの20℃培養抽出物 のポリガラクツロナーゼ活性画分からポリガラクツロナーゼⅠを単離した。ポリガラクツ ロナーゼⅠ精製標品(P4-4-2)の比活性は3 662U/mgであり、粗抽出液から全体として116倍に 精製した。精製標品はポリアクリルアミドゲル電気泳動によって、均一であることが示さ れた。また、SDS存在下ポリアクリルアミドゲル電気泳動によって本酵素の分子質量は 39.8±1.10kDa(S.E.)、さらに等電点電気泳動によって本酵素の等電点はpH8.70と見積もられた。 Polygaracturonasehas been isolated from the mesophilic white mold Sclerotinia Sclerotiorum by cation-exchange chromatography on a column of CM-Toyopearl followed by gel filtration on a column of Sephacryl S-200. Purified enzyme preparation(P4-4-2) had a specific activity of 3 662 units per mg of protein, that is, 116-fold purification over the crude extract of the wheat bran culture of the mesophilic fungus S.... |
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Palavras-chave: Sclerotinia sclerotiorum; 常温菌; 菌核病菌; ポリガラクツロナーゼ; 細胞壁分解酵素; Mesophile; White mold; Polygalacturonase; Cell wall degrading enzyme. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/106 |
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| KAWASHIMA, Chiho; MUNAKATA, Megumi; SHIMIZU, Takashi; MIYAMOTO, Akio; KIDA, Katsuya; MATSUI, Motozumi; 川島, 千帆; 清水, 隆; 宮本, 明夫; 木田, 克弥; 松井, 基純. |
| This study aimed to investigate the effects of insulin resistance (IR) during the close-up dry period on the metabolic status and performance of dairy cows as well as to determine the effects on body weight (BW) and metabolic status of their calves. An insulin tolerance test (ITT) was conducted by administering 0.05 IU/kg BW of insulin to 34 multiparous Holstein cows at 3 weeks prepartum. Blood samples were collected at 0, 30, 45 and 60 min after insulin injection, and cows were divided into two groups based on the time required for glucose to reach the minimum levels [non-IR (NIR), 45 min (n=28); and IR, 60 min (n=6)]. Blood or milk sampling and body condition score (BCS) estimation were performed twice weekly during the experimental period. Blood samples... |
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Palavras-chave: Dairy cow; Dry period; Insulin tolerance test; Metabolic status; Postpartum performance. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4363 |
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| 平田, 昌弘; 板垣, 希美; 内田, 健治; 花田, 正明; 河合, 正人; HIRATA, Masahiro; ITAGAKI, Nozomi; UCHIDA, Kenji; HANADA, Masaaki; KAWAI, Masahito. |
| 本研究は，BC1200～BC300年頃に編纂されたVeda文献/Pāli聖典をテキストに用い，古代インドの乳製品を再現・同定し，それらの乳加工技術の起原について推論することを目的とした．再現実験の結果，dadhi/dadhiは酸乳，navanīta/navanīta・nonītaはバター，takra/takkaはバターミルク，ājya/—はバターオイル，āmikṣā/—はカッテージチーズ様の乳製品，vājina/—はホエイと同定された．sarpiṣ/sappihaはバターオイル，sarpirmaṇḍa/sappimaṇḍaはバターオイルからの唯一派生する乳製品として低級脂肪酸と不飽和脂肪酸の含有量が多い液状のバターオイルであると類推された．Veda文献・Pāli聖典は，「kṣīra/khīraからdadhi/dadhiが，dadhi/dadhiからnavanīta/navanītaが，navanīta/navanītaからsarpiṣ/sappiが，sarpiṣ/sappiからsarpirmaṇḍa/sappimaṇḍaが生じる」と説明する．再現実験により示唆されたことは，この一連の加工工程は「生乳を酸乳化し，酸乳をチャーニングしてバターを，バターを加熱することによりバターオイルを加工し，静置することにより低級脂肪酸と不飽和脂肪酸とがより多く含有した液状のバターオイルを分離する」ことである．さらに，ユーラシア大陸の牧畜民の乳加工技術の事例群と比較検討した結果，Veda文献・Pāli聖典に記載された乳加工技術の起原は西アジアであろうことが推論された. |
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Palavras-chave: Historical document; Indo-Aryan; Milk product; Reproduction experiment; South Asia. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3864 |
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| Registros recuperados: 4.144 | |
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