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| Registros recuperados: 2.463 | |
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| Borges,Andreia A.; Humann,Fernanda C.; Campos,Lucio A. Oliveira; Tavares,Mara G.; Hartfelder,Klaus. |
| In Hymenoptera, homozygosity at the sex locus results in the production of diploid males. In social species, these pose a double burden by having low fitness and drawing resources normally spent for increasing the work force of a colony. Yet, diploid males are of academic interest as they can elucidate effects of ploidy (normal males are haploid, whereas the female castes, the queens and workers, are diploid) on morphology and life history. Herein we investigated expression levels of ten caste-related genes in the stingless bee Melipona quadrifasciata, comparing newly emerged and 5-day-old diploid males with haploid males, queens and workers. In diploid males, transcript levels for dunce and paramyosin were increased during the first five days of adult... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stingless bee; Real time PCR; Caste; Diploid male; Differential gene expression. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400025 |
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| Bevilacqua,Roberta A.U.; Corvello,Cassandra M.; Duarte,Ana Paula; Simpson,Andrew J.G.. |
| A crucial role for the adenomatous polyposis colonic (APC) gene in colorectal carcinogenesis has been conclusively established, but, the role of APC in gastric tumors remains controversial. APC mutations have been detected at a relatively high frequency in gastric tumors of Japanese patients, yet such mutations have been reported to be extremely rare in British patients and patients from north-central-Italy. We here report the analysis of 40 primary sporadic gastric adenocarcinomas and 35 primary sporadic colon adenocarcinomas (from patients resident in São Paulo, Brazil), for mutations in the APC gene between codons 686 and 1693 using the protein truncation test. Although 19 truncating mutations were detected in 35 colon adenocarcinomas (54.2%) none were... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200001 |
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| Oshiro,Elisa E.; Tavares,Milene B.; Suzuki,Celso F.; Pimenta,Daniel C.; Angeli,Claudia B.; Oliveira,Julio C.F. de; Ferro,Maria I.T.; Ferreira,Luis C.S.; Ferreira,Rita C.C.. |
| In this study we investigated the prevalence of the oppA gene, encoding the oligopeptide binding protein (OppA) of the major bacterial oligopeptide uptake system (Opp), in different species of the genus Xanthomonas. The oppA gene was detected in two Xanthomonas axonopodis strains among eight tested Xanthomonas species. The generation of an isogenic oppA-knockout derivative of the Xac 306 strain, showed that the OppA protein neither plays a relevant role in oligopeptide uptake nor contributes to the infectivity and multiplication of the bacterial strain in leaves of sweet orange (Citrus sinensis) and Rangpur lime (Citrus limonia). Taken together these results suggest that the oppA gene has a recent evolutionary history in the genus and does not contribute... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xanthomonas; Xac; OppA; Oligopeptide uptake system; ABC transporter. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200023 |
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| Nogueira,Douglas Willian; Maluf,Wilson Roberto; Figueira,Antonia dos Reis; Maciel,Gabriel Mascarenhas; Gomes,Luiz Antonio Augusto; Benavente,Cesar Augusto Ticona. |
| The aim was to assess heterosis in a set of 16 summer-squash hybrids, and evaluate the combining capacity of the respective parental lines, which differed as to the degree of parthenocarpy and resistance to PRSV-W (Papaya Ringspot Virus-Watermelon strain). The hybrids were obtained using a partial diallel cross design (4 x 4). The lines of parental group I were 1 = ABX-037G-77-03-05-01-01-bulk, 2 = ABX-037G-77-03-05-03-10-bulk, 3 = ABX-037G77-03-05-01-04-bulk and 4 = ABX-037G-77-03-05-05-01-bulk, and of group II, 1' = ABX-037G-77-03-05-04-08-bulk, 2' = ABX-037G-77-03-05-02-11-bulk, 3' = Clarice and 4' = Caserta. The 16 hybrids and eight parental lines were evaluated for PRSV-W resistance, parthenocarpic expression and yield in randomized complete-block... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucurbita pepo; Heterosis; Hybrids; Potyvirus; Papaya ringspot virus. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400014 |
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| Mendes-Bonato,Andréa Beatriz; Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do. |
| Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (R genome) and a natural apomictic tetraploid accession of B. brizantha (B genome). Chromosomes associated predominantly as bivalents. From this phase to the end of meiosis, chromosomes presented irregular segregation and abnormal arrangement in the metaphase plate. During metaphase I, in 27.8% of meiocytes, bivalents were distributed in two metaphase plates. In anaphase I, two distinct and typical bipolar spindles were formed. In 29.7% of pollen mother cells, one genome did not divide synchronically, with chromosomes lagging behind or not segregating at all. The second division was very irregular, resulting in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria; Forage grass; Interspecific hybridization; Meiotic rhythm; Microsporogenesis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400021 |
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| Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo. |
| The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018 |
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| Nascimento,Moysés; Cruz,Cosme Damião; Peternelli,Luiz Alexandre; Campana,Ana Carolina Mota. |
| The efficiency of simulated annealing algorithms and rapid chain delineation in establishing the best linkage order, when constructing genetic maps, was evaluated. Linkage refers to the phenomenon by which two or more genes, or even more molecular markers, can be present in the same chromosome or linkage group. In order to evaluate the capacity of algorithms, four F2 co-dominant populations, 50, 100, 200 and 1000 in size, were simulated. For each population, a genome with four linkage groups (100 cM) was generated. The linkage groups possessed 51, 21, 11 and 6 marks, respectively, and a corresponding distance of 2, 5, 10 and 20 cM between adjacent marks, thereby causing various degrees of saturation. For very saturated groups, with an adjacent distance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Better order; Genetic mapping; Genomic analyses; Stochastic optimization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200032 |
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| Hiragi,Cássia de Oliveira; Miranda-Vilela,Ana Luisa; Rocha,Dulce Maria Sucena; ,Silviene Fabiana de; Hatagima,Ana; Klautau-Guimarães,Maria de Nazaré. |
| Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution of these variants. We described the distribution frequency of CAT (21A/T), SOD2 (Ala9Val), GPX1 (Pro198Leu), GSTM1 and GSTT1 polymorphisms in three Brazilian population groups: Kayabi Amerindians (n = 60), Kalunga Afro-descendants (n = 72), and an urban mixed population from Federal District (n = 162). Frequencies of the variants observed in Kalunga... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; PCR-RFLP; Gene polymorphisms; Brazilian ethnicities; Population genetics; Pharmacogenetics. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100003 |
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| Jolodar,Abbas; Miller,David J.. |
| Two constructions in pHS164 vector were designed to permit expression of OV7A and OV4A inserts encoding the N-terminal and C-terminal portion of an aspartic protease from Onchocerca volvulus, respectively. A novel 39 kD protein ompA-OV7A fusion protein was stably expressed as ompA fusion in a modified strain of Salmonella typhimurium strain SL5000 and E.coli strain JM109. Expression of the fusion protein in bacterial strains harboring the constructs were evaluated by western blotting. E.coli and Salmonella lysates were fractionated by 10% SDS-PAGE gel and then immobilized to nitrocellulose membrane by electroblotting. Primary polyclonal antibody generated in rats against the GST-OV7A fusion protein was used in the Western blots. It remains to be seen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Filarial parasite; Nematode; Salmonella typhimurium. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100018 |
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| Osorio,Marina Borges; Bücker-Neto,Lauro; Castilhos,Graciela; Turchetto-Zolet,Andreia Carina; Wiebke-Strohm,Beatriz; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia. |
| Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drought; Gene expression; Glycine max; Phylogeny; Plant-microbe interactions. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200005 |
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| Registros recuperados: 2.463 | |
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