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| Registros recuperados: 2.860 | |
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| Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da. |
| Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025 |
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| Ferreira,Suelen Regina; Murakami,Alice Eiko; Silveira,Thais Gomes Verzignassi; Santos,José Maurício Gonçalves dos; Fernandes,Jovanir Inês Muller. |
| The aim of this work was to evaluate the effect of broiler breeders' age and yeast wall (YW) levels on broilers' performance and macrophage activitys. The experiment consisted in a completely randomized design and a 2 X 5 factorial arrangement and two controls of two broiler breeders age (34 and 57 weeks of age) and five YW levels (zero, one, two, three or four kg of YW/ton of diet). They received sorghum diet compared to a control corn/soybean meal diet. The age of the broiler breeders influenced the performance, but did not affect macrophage activity. At 34 weeks age broiler breeders' progeny, all diets were similar considering the average weight. For 57 weeks age broiler breeders' progeny, at 21 days, only average weight of sorghum diet supplemented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcass yield; Mananoligossacarydes; Phagocytosis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200019 |
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| Anselmini,Justina Inês; Zanette,Flávio. |
| The objectives of this work were to describe the development and growth rate of the pine cones of Araucaria angustifolia during and after the pollination period, to identify the period of maximum growth of the cones and the period of pollination and maturation of the pine nuts. The adult individuals were found at the Setor de Ciencias Agrarias of the UFPR, in Curitiba - PR. Collections and measurements of pine cones were made in 2003 and 2005. The diameter and the length of the pine cones were measured 15 different times in both the years. In the studied environmental conditions, the period between the pollination and maturation of the pine nuts was 20 months. The maximum pine cone growth was achieved between October and January after the pollination, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parana-pine; Strobili; Pollination. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400003 |
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| Lima,Eleonidas Moura; Lopes,Otávio Sérgio; Soares,Leonardo Ferreira; Arruda,Talitta Dantas; Gigek,Carolina Oliveira; Melo,Cynthia Germoglio Farias; Smith,Marilia Arruda Cardoso; Oliveira,João Ricardo Gonçalves; Medeiros,Arnaldo; Delatorre,Plínio; Burbano,Rommel Rodriguez. |
| Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DSASP method; Genotyping; Gastric Cancer. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414 |
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| Pires,Ana Christina Duarte; Melo,Vander de Freitas; Lima,Valmiqui Costa; Motta,Antônio Carlos Vargas. |
| The aim of this work was to evaluate the mineralogical and chemical characteristics of most representative soils of the Region of Curitiba, Paraná State. Samples were collected at different depths. The results showed: (a) the quartz was the only identified mineral at the silt and sand fractions. The dominant clay mineral was Kaolinite, with contents ranging from 676.7 to 820.8 g kg-1. The gibbsite was also an important constituent of the most weathered horizons and the hematite and goethite contents were low, mainly in the Histosol; (b) at the C horizon of the Inceptisol, high intensity of vermiculite/smectite reflections were detected (X-ray diffraction), justifying the high capacity of expansion and contraction, normally showed for this soil horizon; (c)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kaolinite; Crystalline and amorphous Fe and Al oxides; 2:1 minerals; XRD. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200001 |
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| Al-Olayan,Ebtesam M; Mohamed,Aly F; El-Khadraqy,Manal F; Shebl,Rania I; Yehia,HanryM. |
| ABSTRACT Vaccine improvement depends on the formulation, adjuvant type and inactivant used. The type of formulation may interfere with immunogenicity. The present work aimed to evaluate the inactivation activity and related immune potential of the Cobra venom-derived LAO enzyme compared to the currently used inactivants (BPL and formalin) for both animal and human vaccines. The RVF virus was completely inactivated within 6 hrs, 4 hrs and 2 hrs after treatment with Formalin, LAO and BPL, respectively. The vaccine potency [ED50] was arranged in a descending order from formalin (0.016) to BPL (0.005) and LAO (0.002). The total IgG levels, Neutralizing Index (NI) and Interferon levels were significantly increased compared to those detected after immunization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rift Valley fever; Inactivant; Humoral immunity; Interferon; Interleukins; LAO. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100345 |
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| Registros recuperados: 2.860 | |
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