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Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports BJM
Przystaś,Wioletta; Zabłocka-Godlewska,Ewa; Grabińska-Sota,Elżbieta.
Abstract Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization. The best growth of fungal strains was observed on the washer, brush, grid and sawdust supports. Based on the results of dye adsorption, the brush and the washer were selected for further study. These solid supports adsorbed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Decolorization; Fungi; Azo dyes; Triphenylmethane dyes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200285
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Microbial populations of crude oil spill polluted soils at the Jordan-Iraq desert (the Badia region) BJM
Saadoun,Ismail; Mohammad,Munir J.; Hameed,Khalid M.; Shawaqfah,Mo'ayyad.
Microbial populations' inhabitants in crude petroleum contaminated soils were analyzed in relation with the soil characteristics. A noticeable greater decline of bacterial counts and diversity but a prevalence of the genus Pseudomonas over the other identified genera in the fresh contaminated soils as compared to the old ones was observed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Crude oil; Fungi; Soil; Streptomycetes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300008
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Isolation of fungi from dung of wild herbivores for application in bioethanol production BJM
Makhuvele,Rhulani; Ncube,Ignatious; Jansen van Rensburg,Elbert Lukas; La Grange,Daniël Coenrad.
ABSTRACT Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides. Due to the presence of residual partially degraded lignocellulose in the gut, the dung of herbivores can be considered as a natural source of pre-treated lignocellulose. A total of 101 fungi were isolated (36 yeast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignocellulase; Biofuel; Fungi; Xylose; Fermentation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400648
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Dermatophytes and other associated fungi in patients attending to some hospitals in Egypt BJM
Abd Elmegeed,Al Shimaa M.; Ouf,S.A.; Moussa,Tarek A.A.; Eltahlawi,S.M.R..
Dermatophytes are keratinophilic fungi that infect keratinized tissues causing diseases known as dermatophytoses. Dermatophytes are classified in three genera, Epidermophyton, Microsporum, and Trichophyton. This investigation was performed to study the prevalence of dermatomycosis among 640 patients being evaluated at the dermatology clinics at Kasr elainy, El-Husein and Said Galal hospitals in Cairo and Giza between January 2005 and December 2006. The patients were checked for various diseases. Tinea capitis was the most common clinical disease followed by tinea pedis and tinea corporis. Tinea cruris and tinea unguium were the least in occurrence. Tinea versicolor also was detected. The most susceptible persons were children below 10 years followed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytosis; Dermatophytes; Fungi; Keratinophilic; Prevalence; Dermatology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300799
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Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil BJM
Silva,Lidiane Roberta Cruz da; Souza,Odacy Camilo de; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Coelho,Rosalie Reed Rodrigues; Souza-Motta,Cristina Maria.
Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shrimp; Fungi; Water; Protease; Aflatoxin B.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100007
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Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis BJM
Tigano,Myrian Silvana; Aljanabi,Salah; Mello,Sueli Corrêa Marques de.
The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities computed from RAPD markers showed two distinct genetic groups of isolates related to both species. RAPD analysis proved to be an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in S. obtusifolia, and also for distinguishing Alternaria species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Senna obtusifolia; Bioherbicide; Microbial control.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200005
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Fusarium and Aspergillus mycotoxins contaminating wheat silage for dairy cattle feeding in Uruguay BJM
del Palacio,Agustina; Bettucci,Lina; Pan,Dinorah.
Abstract Wheat is one of the most important cultivated cereals in Uruguay for human consumption; however, when harvest yields are low, wheat is usually used in ensiling for animal feeding. Ensiling is a forage preservation method that allows for storage during extended periods of time while maintaining nutritional values comparable to fresh pastures. Silage is vulnerable to contamination by spoilage molds and mycotoxins because ensilage materials are excellent substrates for fungal growth. The aim of the study was to identify the mycobiota composition and occurrence of aflatoxins and DON from wheat silage. A total of 220 samples of wheat were collected from four farms in the southwest region of Uruguay were silage practices are developed. The main fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat silage; Mycotoxins; Fungi.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401000
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Mycotoxicological and palynological profiles of commercial brands of dried bee pollen BJM
Deveza,Michele Valadares; Keller,Kelly Moura; Lorenzon,Maria Cristina Affonso; Nunes,Lucila Maria Teixeira; Sales,Érika Oliveira; Barth,Ortrud Monika.
Abstract Pollen is used in the human diet as a food supplement because of its high nutritional value; however, this product is prone to fungal contamination that could potentially generate toxins that are harmful to human health. This study aimed to verify the floral diversity of commercial brands of bee pollen and their mycotoxicological safety for human consumption. A total of 27 bee pollen samples were analyzed; these samples represented commercial brands, either showing an inspection seal or not, marketed in the State of Rio de Janeiro. The analyzed parameters included floral diversity through palynological analysis, water activity, fungal counts, identification and toxigenic profiles. The palynological analysis identified nine plant families, of which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bee products; Fungi; Mycotoxins; Pollen loads; Public health.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401171
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Microbiological monitoring mf mineral water commercialized in Brazil BJM
Pontara,André Venturini; Oliveira,Christianne Dezuani Dias de; Barbosa,Amir Horiquini; Santos,Rafael Aparecido dos; Pires,Regina Helena; Martins,Carlos Henrique Gomes.
The quality of mineral water commercialized in Brazil regarding the microbial content was analyzed and the results were compared with the standards established by the current legislation. Results demonstrated there was no bacterial contamination, but several types of fungi were found. Therefore, bottled mineral water could be considered a possible route for the transmission of filamentous fungi and yeasts.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mineral water; Microbial contamination; Membrane filter technique; Fungi.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200020
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Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis BJM
Gontia-Mishra,Iti; Deshmukh,Dhanshree; Tripathi,Niraj; Bardiya-Bhurat,Khushboo; Tantwai,Keerti; Tiwari,Sharad.
Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytase; Phytate; Fungi; 18S rRNA gene; Aspergillus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100046
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Biodegradation of polycyclic aromatic hydrocarbons by soil fungi BJM
Clemente,Andrea R.; Anazawa,Tania A.; Durrant,Lucia R..
Thirteen deuteromycete ligninolytic fungal strains were grown in media containing polycyclic aromatic hydrocarbons (PAHs), for 6 and 10 days. The PAHs were added directly with the inocula or on the third day of cultivation. A selection of the best strains was carried out based on the levels of degradation of the PAHs and also on the ligninolytic activities produced by the fungi. The selected strains were cultivated for 3, 6, 9, 12 and 15 days in the PAHs-containing media. Degradation of PAHs, as measured by reversed-phase HPLC on a C18 column, varied with each strain as did the ligninolytic enzymes present in the culture supernatants. Highest degradation of naphthalene (69%) was produced by the strain 984, having Mn-peroxidase activity, followed by strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Ligninolytic enzymes; Polycyclic aromatic hydrocarbons; Fungi.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400001
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Microscopic fungi in the Atlantic Rainforest in Cubatão, São Paulo, Brazil BJM
Schoenlein-Crusius,Iracema Helena; Milanez,Adauto I.; Trufem,Sandra F.B.; Pires-Zottarelli,Carmen L.A.; Grandi,Rosely A. Piccolo; Santos,Maria L.; Giustra,Kátia C..
This article presents a survey of fungi obtained from soil, water and mixed leaf litter samples taken from the Atlantic Rainforest in the municipality of Cubatão, in the State of São Paulo, during the years of 1993 to 1995. Using different techniques for the isolation of microscopic fungi, a total of 280 taxa was obtained (66 zoosporic fungi, 40 Mucorales, 45 Glomales, 125 anamorphs, three Ascomycota and one Basidiomycota), with 23 species being reported for the first time in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Diversity; Atlantic Rainforest; Brazil.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300014
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A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies BJM
Burgdorf,R.J.; Laing,M.D.; Morris,C.D.; Jamal-Ally,S.F..
Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA) and non-metric multidimensional scaling (NMDS) were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM). Yeast band analysis of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophyte; Fungi; DNA; Surface sterilization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300030
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Long-term cryopreservation of basidiomycetes BJM
Linde,Giani Andrea; Luciani,Alana; Lopes,Ana Daniela; Valle,Juliana Silveira do; Colauto,Nelson Barros.
Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures. Therefore, this study aimed to collect information on the main conditions for long-term cryopreservation of basidiomycetes in the last 20 years. Scientific articles on cryopreservation of basidiomycetes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Preservation; Substrate; Fungi; Mycelial viability; Cryoprotectant.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200220
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Fungal flora of the digestive tract of Rhodnius prolixus, Rhodnius neglectus, Diptelanogaster maximus and Panstrongylus megistus, vectors of Trypanosoma cruzi, Chagas, 1909 BJM
Moraes,Aurea Maria Lage de; Junqueira,Angela Cristina Vieira; Celano,Valéria; Costa,Gisela Lara da; Coura,José Rodrigues.
This paper reports a study on the mycobiota in the digestive tract of four important species of triatomines: Rhodnius prolixus, R. neglectus, Diptelanogaster maximus and Panstrongylus megistus. The digestive tracts of 90 adults and 425 nymphs of these four triatomine species were studied and 365 fungal strains were isolated. The genera with the greatest number of species were Aspergillus, Penicillium (14 species in each genus), Acremonium and Cladosporium (three species in each genus), and the most frequent species, in decreasing order, were Aspergillus awamori, Penicillium corylophilum, Cladosporium herbarum and Aspergillus niger. It was concluded that, among the isolated fungi, Aspergillus niger and Penicillium corylophilum might be part of the natural...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus spp; Fungi; Insect's digestive tract; Mycoflora; Penicillium spp; Triatomines.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300003
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Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin BJM
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Nascimento,João Paulo; Laranjeira,Delson.
Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Fungi were evaluated according to their capacity to hydrolyse inulin and the variation in the diversity of these fungi during the plant's life cycle was also accessed. Forty-nine species of filamentous fungi were isolated. Penicillium and Aspergillus were the genera that presented higher number of species, nine and seven, respectively. At the end of the sunflower life cycle, cultivated both in field and in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Rhizosphere; Soil; Inulinase; Sunflower.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300018
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The occurrence of molds, yeasts and mycotoxins in pre-cooked pizza dough sold in Southern Rio Grande do Sul BJM
Pinho,Beatriz Helena; Furlong,Eliana Badiale.
The quality of pre-cooked pizza dough was investigated by assessing the occurrence of molds, yeasts and mycotoxins. Random sampling of commercial pre-cooked pizza cakes was done in different stores in the cities of Rio Grande and Pelotas, RS, between 1996 and 1997. The products were analysed on the sampling day and after storage at room (22-30ºC) or refrigerated temperature (7ºC) following the shelf life stated by the manufacturer (25,30 and 45 days). The results showed that mold and yeast contamination was frequently above the maximum limits (10³CFU/g-1) established by Brazilian guide lines, even in samples kept at refrigerated temperatures up to the end of shelf life. Although no mycotoxin contamination was detected, a strain of the Penicillium genus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pre-cooked pizza dough; Fungi; Mycotoxins.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200007
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Microbial diversity in Brazilian mangrove sediments: a mini review BJM
Ghizelini,Angela Michelato; Mendonça-Hagler,Leda Cristina Santana; Macrae,Andrew.
The importance and protection of mangrove ecosystems has been recognized in Brazilian Federal law since 1965. Being protected in law, however, has not always guaranteed their protection in practice. Mangroves are found in coastal and estuarine locations, which are prime real estate for the growth of cities, ports and other economic activities important for Brazilian development. In this mini-review we introduce what mangroves are and why they are so important. We give a brief overview of the microbial diversity found in mangrove sediments and then focus on diversity studies from Brazilian mangroves. We highlight the breadth and depth of knowledge about mangrove microbial communities gained from studying Brazilian mangroves. We report on the exciting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangroves; Biodiversity; Bacteria; Fungi; Brazil; Conservation; Ecology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400002
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Risk assessment of the occurrence of aflatoxin and fungi in peanuts and cashew nuts BJPS
Kujbida,Paula; Maia,Patrícia Penido; Araújo,Ariadne Naama de; Mendes,Leonardo Daniel; Oliveira,Mariana Lepri de; Silva-Rocha,Walicyranison Plinio; Brito,George Queiroz de; Chaves,Guilherme Maranhão; Martins,Isarita.
In the present study, the occurrence of fungi and aflatoxins (AFs) in peanut and cashew nut samples was investigated. Mycological analysis revealed the presence of fungi in 58.8% of samples, and assessment of AFs by chromatographic methods revealed that 52.9% were contaminated by AFs. AFB1 was the principal component in all AF-contaminated samples, with a mean level of 14.0, and 1.08 µg/kg in peanut and cashew nut, respectively. Eleven samples (32.4%) exceeded the total AF maximum level (4 μg/kg) and 8 samples (23.5%) exceeded the AFB1 (2 μg/kg) established by the European Commission. Our findings suggest that the incidence of AFs emphasizes the need for regular monitoring and a more stringent food safety system to control AFs at the lowest possible levels...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Peanut; Cashew nut; Fungi; Risk assessment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100502
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Effect of powdered spice treatments on mycelial growth, sporulation and production of aflatoxins by toxigenic fungi Ciência e Agrotecnologia
Chalfoun,Sára Maria; Pereira,Marcelo Cláudio; Resende,Mario Lúcio V.; Angélico,Caroline Lima; Silva,Rozane Aparecida da.
The effect of ten powdered spice plants was evaluated at the concentration of 1, 2, 3 and 4% to observe the mycelial growth and sporulation of Aspergillus niger and Eurotium repens. The spices were added to the culture media PDA and CYA20S. Clove completely inhibited the mycelial growth of the tested fungi. The other spices: cinnamon, garlic, thyme, mint, anis, oregano and onion were, in a decreasing order, promising antifungals. Bay leaf and basil did not show a pronounced fungistatic effect. The antitoxigenic potential of the spices was tested against one aflatoxin-producing strain of AspergiIIus flavus. The spices were tested at the same concentrations previously mentioned and were added to the culture medium YES, appropriate for the production of those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spices; Aflatoxins; Control; Fungi.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000400018
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