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Registros recuperados: 341
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Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124) BABT
Mendonça,Analucia Longman; Mariano,Rosa de Lima Ramos; Araújo,Janete Magali de; Cavalcante,Uided Maaze T..
Forty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Genetic improvement; Bacillus spp.; B. cereus; Esterase.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011
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New aspects on atrazine biodegradation BABT
Sene,Luciane; Converti,Attilio; Secchi,Geslaine Aparecida Ribeiro; Simão,Rita de Cássia Garcia.
The world practice of using agrochemicals for long periods, in an indiscriminated and abusive way, has been a concern of the authorities involved in public health and sustainability of the natural resources, as a consequence of environmental contamination. Agrochemicals refer to a broad range of insecticides, fungicides and herbicides, and among them stands out atrazine, a herbicide intensively used in sugarcane, corn and sorghum cultures, among others. Researches have demonstrated that atrazine has toxic effects in algae, aquatic plants, aquatic insects, fishes and mammals. Due to the toxicity and persistence of atrazine in the environment, the search of microbial strains capable of degrading it is fundamental to the development of bioremediation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atrazine; Biodegradation; Bacteria; Fungi; Microbial consortium.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200030
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Indoor Air Quality in the Hospital: The Influence of Heating, Ventilating and Conditioning Systems BABT
Božić,Jelena; Ilić,Predrag; ilić,Svetlana.
Abstract Clean, fresh air is the most important requirement for good indoor air quality (IAQ) in all buildings, but it is especially important with regard to the environments within hospitals and other healthcare facilities. The literature indicates that buildings with heating, ventilating and air conditioning (HVAC) systems may have an increased risk of sick building syndrome (SBS) and building-related illness (BRI) if they are not well maintained. Microorganisms are brought into hospitals by people, air currents, water, construction materials and equipment. The main objective of this study was to assess the degree of fungal and bacterial contamination in the University Clinical Centre in Banja Luka, which is directly connected to the HVAC system....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indoor air quality; Hospital; HVAC system; Bacteria; Fungi.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100607
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Antifouling effect of bioactive compounds from marine sponge Acanthella elongata and different species of bacterial film on larval attachment of Balanus amphitrite (cirripedia, crustacea) BABT
Ganapiriya,Viswambaran; Maharajan,Athisuyambulingam; Kumarasamy,Pothiappan.
The antifouling activity of bioactive compounds from marine sponge Acanthella elongata (Dendy) and five species of bacterial biofilm were studied. Larvae of Balanus amphitrite (Cyprids and nauplii) were used to monitor the settlement inhibition and the extent to which inhibition was due to toxicity. The crude extract and partially purified fractions of A.elongata showed significant inhibition over the settlement individually, and with the interaction of bacterial species. No bacterial film stimulated the barnacle settlement. The high but variable levels of antifouling activity in combination with less amount of toxicity showed the potential of these metabolites in environmentally-friendly antifouling preparations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofouling; Bacterial film; Bacteria; Barnacles; Cyprids; B. amphitrite.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300010
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Decontamination of Pig Carcasses Using Water Pressure and Lactic Acid BABT
Brustolin,Jean Carlos; Dal Pisol,Andreia; Steffens,Juliana; Toniazzo,Geciane; Valduga,Eunice; Di Luccio,Marco; Cansian,Rogério Luis.
The objective of this work was to evaluate the effect of different water pressures and concentrations of lactic acid on microbial counts (mesophilic bacteria, enterobacteria and Salmonella) on pig carcasses without contamination and contaminated carcasses, before and after the last shower and before being cooled. The tests were carried out using 4, 3 and 2 bar water pressure, and 2, 1 and 0% lactic acid concentration. In general, both the pressure in the shower and lactic acid had a positive effect by reducing the microbial count. The interaction between the pressure and lactic acid caused the largest reduction in carcasses surface count for mesophiles. With regard to enterobacteria on contaminated carcasses, the most important variable was the lactic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig carcasses; Decontamination; Water pressure; Lactic acid; Bacteria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600954
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Hospital gowns as a vehicle for bacterial dissemination in an intensive care unit BJID
Pilonetto,Marcelo; Rosa,Edvaldo Antonio Ribeiro; Brofman,Paulo Roberto Slud; Baggio,Daniela; Calvário,Francine; Schelp,Cristiane; Nascimento,Aguinaldo; Messias-Reason,Iara.
The microbiota from the uniforms of 31 professionals from the general intensive care unit was analyzed. The samples were collected in duplicate at the beginning and at the end of the work period. Total viable counts of microorganisms were determined; there was a significant increase in the counts at the end of the period, when compared with those obtained at the beginning. No significant difference was observed between the first and second counts obtained from the cuffs. However, differences were observed for the samples from the abdominal region. Among the isolated pathogens 11/18 were Staphylococcus aureus, 2/18 were Acinetobacter baumannii, 2/18 were Klebsiela pneumoniae and 1/18 were Serratia rubidae. Some of these isolates were multi-resistant to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gown; Bacteria; Hospital infection; Cross contamination.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300003
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Morbimortality study of infection in patients undergoing different types of dialysis in a renal replacement therapy center BJID
Curty,Natália Fabiane Ridão; Martins,Lucilene Fagundes da Silva; Ito,Carmen Antônia Sanches; Schafranski,Marcelo; Brites,Dorelayne Aparecida; Busato,César Roberto.
INTRODUCTION: Renal replacement therapy is the treatment of end-stage chronic kidney disease and can be performed through dialysis catheters, arteriovenous fistulas/grafts, and peritoneal dialysis. Patients are usually immunocompromised and exposed to invasive procedures, leading to high rates of infection and increased mortality.OBJECTIVES: To compare the prevalence of infection and related deaths, as well as the sensitivity profile of the putative bacteria in patients treated with peritoneal dialysis, arteriovenous fistula hemodialysis and catheter hemodialysis.METHODS: This is case-control study. Six hundred forty-four patients undergoing renal replacement therapy were selected. Patients were divided into three groups according to the modality of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hospital infection; Morbimortality; Renal replacement therapy center; Hemodialysis; Bacteria; Sensitivity profiles.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300281
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Evaluation of different detection methods of biofilm formation in the clinical isolates BJID
Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad.
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002
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Catheter-related infections in a northwestern São Paulo reference unit for burned patients care BJID
Campos Júnior,Cláudio Penido; Sanches,Patrícia; Tedokon,Elizabete Aparecida; Souza,Ana Carolina Remondi; Machado,Ricardo Luiz Dantas; Rossit,Andréa Regina Baptista.
Despite improvements in care and rehabilitation of burned patients, infections still remain the main complication and death cause. Catheter-related infections are among the four most common infections and are associated with skin damage and insertion site colonization. There are few studies evaluating this kind of infection worldwide in this special group of patients. Padre Albino Hospital Burn Care Unit (PAHBCU) is the only reference center in the Northwestern São Paulo for treatment of burned patients. This paper presents the results of a retrospective study aiming at describing the epidemiological and clinical features of catheter-related infections at PAHBCU.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catheter; Infections; Burns; Bacteria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200009
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Identifying and controlling a multiresistant pseudomonas aeruginosa outbreak in a latin-american cancer centre and its associated risk factors BJID
Cortes,Jorge Alberto; Cuervo,Sonia Isabel; Urdaneta,Ana María; Potdevin,Guillermo; Arroyo,Patricia; Bermúdez,Diana; Correa,Adriana; Villegas,María Virginia.
Pseudomonas aeruginosa is an important and frightening microorganism for patients suffering from cancer. Multiresistant P. aeruginosa (MRPA) may appear as a consequence of exposure to multiple antibiotics or from a breakdown in infection control practices. This article reports an MRPA outbreak in a cancer treatment centre and the consequent case control study. Mechanical ventilation was identified as being the main risk factor for developing MRPA colonisation or infection; molecular analysis confirmed the outbreak. A multifaceted strategy was adopted, involving reinforcing hand-washing practices, contact isolation, antibiotic restriction and suction devices for mechanically-ventilated patients. MRPA was controlled and the outbreak ended. Such strategy may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Epidemiology; Multiple drug resistance; Bacteria; Risk factor.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200005
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Bacterial contamination in milk kitchens in pediatric hospitals in Salvador, Brazil BJID
Cairo,Romilda Castro; Silva,Luciana Rodrigues; Andrade,Carol Ferreira de; Barberino,Maria Goreth de Andrade; Bandeira,Antônio Carlos; Santos,Kleber Pimentel; Diniz-Santos,Daniel R..
Milk may represent an important source of infectious agents to hospitalized pediatric patients. To describe the bacterial microflora isolated from the hands, stools, pharynx of all workers at milk kitchens in pediatric hospitals in the city of Salvador, Brazil, as well as in the formulas prepared by them, we carried out this cross-sectional study with all 91 workers from the 20 milk kitchens of all the public and private hospitals in Salvador, Brazil. Hand and pharynx swabs and stool samples were collected from all workers, as well as samples of the milk and formulas delivered by the kitchens. All samples were cultured for the detection of pathogenic and non-pathogenic bacteria. Pathogenic bacteria were isolated from 20 (22.0%) and 8 (8.8%) cultures of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Microflora; Hands; Faeces; Pharynx; Milk; Contamination; Hospital; Children.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300011
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Trends in bacterial resistance in a tertiary university hospital over one decade BJID
Góngora Rubio,Fernando; Oliveira,Viviane Decicera Colombo; Rangel,Regina Mara Custódio; Nogueira,Mara Corrêa Lelles; Almeida,Margarete Teresa Gottardo.
The objective of this study was to investigate bacterial resistance trends, infection sites and the relationship between resistance and admittance to the intensive care unit (ICU). A total of 53,316 bacteria identified between 1999 and 2008 were evaluated. Multidrug resistance was characterized when gram-negative bacilli (GNB) presented resistance to two or more classes of antibiotics. Gram-positive cocci (CPC) were assessed for resistance to penicillin, oxacillin and vancomycin. GNB were the most common (66.1%) isolate. There was a 3.7-fold overall increase in multidrug resistant GNB over the study period; Acinetobacter baumanii and Staphylococcus aureus were the most prevalent. Highest increases were recorded for Kleb siella pneumoniae (14.6-fold) and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Multiresistance; Bacteria; Trends.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400014
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IL-6 and IL-8 in cerebrospinal fluid from patients with aseptic meningitis and bacterial meningitis: their potential role as a marker for differential diagnosis BJID
Pinto Junior,Vitor Laerte Laerte; Rebelo,Maria Cristina; Gomes,Rachel Novaes; Assis,Edson Fernandes de; Castro-Faria-Neto,Hugo C; Bóia,Marcio Neves.
Cytokines are molecules that act as mediators of immune response; cerebral spinal fluid (CSF) IL-6 is found in all meningeal inflammatory diseases, but IL-8 is associated with acute bacterial meningitis (ABM). A case control study was done to ascertain the discriminatory power of these cytokines in differentiating ABM from aseptic meningitis (AM); IL-6 and IL-8 CSF concentrations were tested through ELISA in samples collected from patients who underwent investigation for meningitis. Sixty patients, 18 with AM, nine with bacteriologic confirmed ABM and 33 controls, assisted in 2005 (MA and controls) and 2007 (ABM) were included. Differently from controls, IL-6 concentrations were increased both in MA and ABM patients (p < 0.05). CSF IL-8 levels were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CSF; Inflammation meningitis; Aseptic meningitis; Bacteria; IL-6; IL-8.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200011
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Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil BJMBR
Pisa,G.; Magnani,G.S.; Weber,H.; Souza,E.M.; Faoro,H.; Monteiro,R.A.; Daros,E.; Baura,V.; Bespalhok,J.P.; Pedrosa,F.O.; Cruz,L.M..
Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire bacterial communities in the environment. In the present study, we have used this approach to assess the bacterial community in the rhizosphere soil of sugarcane at different times and under different nitrogen fertilization conditions. At the high taxonomic level, few...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rRNA; Biodiversity; Nitrogen fixation; Bacteria; Sugarcane; Soil.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200004
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Comparison of serum PCT and CRP levels in patients infected by different pathogenic microorganisms: a systematic review and meta-analysis BJMBR
Tang,Jun-Hua; Gao,Dong-Ping; Zou,Peng-Fei.
To avoid the abuse and misuse of antibiotics, procalcitonin (PCT) and C-reactive protein (CRP) have been used as new approaches to identify different types of infection. Multiple databases were adopted to search relevant studies, and the articles that satisfied the inclusion criteria were included. Meta-analyses were conducted with Review Manager 5.0, and to estimate the quality of each article, risk of bias was assessed. Eight articles satisfied the inclusion criteria. The concentrations of both PCT and CRP in patients with bacterial infection were higher than those with non-bacterial infection. Both PCT and CRP levels in patients with G− bacterial infection were higher than in those with G+ bacterial infection and fungus infection. In the G+ bacterial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pulmonary infection; PCT; CRP; Bacteria; Fungus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700610
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Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria BJM
Castro,S.B.R.; Leal,C.A.G.; Freire,F.R.; Carvalho,D.A.; Oliveira,D.F.; Figueiredo,H.C.P..
The aim of this work was to evaluate the antibacterial activity of Brazilian plants extracts against fish pathogenic bacteria. Forty six methanolic extracts were screened to identify their antibacterial properties against Streptococcus agalactiae, Flavobacterium columnare and Aeromonas hydrophila. Thirty one extracts showed antibacterial activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant extracts; Bacteria; Fish.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400030
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Bacterioplancton in different tides of the Perequê tidal creek, Pontal do Sul, Paraná, Brazil BJM
Kolm,Hedda Elisabeth; Andretta,Lucimar.
The influence of tidal phase on bacteria abundance in surface waters was studied in five stations along the Perequê tidal creek (Paraná State, Brazil), at spring and neap low and high tides in July 1999. The stations were located from the mouth (St.1) to the creek's head (St. 5). The following parameters were analyzed: salinity, temperature, pH, dissolved oxygen, total carbon dioxide, seston, particulate organic matter, pluviosity, chlorophyll "a", total heterotrophic bacteria, bacterial biomass, cultivable heterotrophic aerobic halophilic and halophobic bacteria, total coliforms and Escherichia coli. The results showed that the highest values of salinity, pH and dissolved oxygen at stations 1 to 4 were registered during the spring high tide. The amount of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Tide; Tidal creek; Perequê; Paraná State.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200002
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Phylogenetic analysis on the soil bacteria distributed in karst forest BJM
Zhou,JunPei; Huang,Ying; Mo,MingHe.
Phylogenetic composition of bacterial community in soil of a karst forest was analyzed by culture-independent molecular approach. The bacterial 16S rRNA gene was amplified directly from soil DNA and cloned to generate a library. After screening the clone library by RFLP, 16S rRNA genes of representative clones were sequenced and the bacterial community was analyzed phylogenetically. The 16S rRNA gene inserts of 190 clones randomly selected were analyzed by RFLP and generated 126 different RFLP types. After sequencing, 126 non-chimeric sequences were obtained, generating 113 phylotypes. Phylogenetic analysis revealed that the bacteria distributed in soil of the karst forest included the members assigning into Proteobacteria, Acidobacteria, Planctomycetes,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karst forest; Bacteria; 16S rRNA gene.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400013
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Protozoans bacterivory in a subtropical environment during a dry/cold and a rainy/warm season BJM
Hisatugo,Karina F.; Mansano,Adrislaine S.; Seleghim,Mirna H.R..
In aquatic ecosystems, bacteria are controlled by several organisms in the food chain, such as protozoa, that use them as food source. This study aimed to quantify the ingestion and clearance rates of bacteria by ciliates and heterotrophic nanoflagellates (HNF) in a subtropical freshwater reservoir (Monjolinho reservoir -São Carlos -Brazil) during one year period, in order to verify their importance as consumers and controllers of bacteria in two seasons, a dry/cold and a rainy/warm one. For this purpose, in situ bacterivory experiments were carried out bimonthly using fluorescently labeled bacteria with 5-(4,6 diclorotriazin-2yl) aminofluorescein (DTAF). Although ciliates have shown the highest individual ingestion and clearance rates, bacterivory was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bacteria; Grazing; Ciliates; Nanoflagellates; Microbial food web.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100020
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Bacterial diversity in soil in response to different plans, phosphate fertilizers and liming BJM
Silva,Paulo da; Nahas,Ely.
The diversity of bacterial isolates from soil in response to different plants (control, Brachiaria ruziziensis and Cajanus cajan), fertilization (control, simple superphosphate and rock phosphate) and liming (with and without lime) was evaluated. Phenotypic and physiological characteristics of the isolates were recorded and organized in a file to identify the bacteria. Among the isolates, 95% were Gram-positive and 5% Gram-negative rods. Soil cultivated with B. ruziziensis favored the nonsporing Gram-positive and Gram-negative rods compared to soils with C. cajan or uncultivated. Number of spore-forming Gram-positive rods were higher in plots with superphosphate than in unfertilized soil or soil fertilized with rock phosphate. In unfertilized plots, larger...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Phosphate; Brachiaria ruziziensis; Cajanus cajan; Liming.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400005
Registros recuperados: 341
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