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| Registros recuperados: 425 | |
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| Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso. |
| Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium; PCR; Identification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013 |
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| Oliveira,L.P.; Cardozo,G.P.; Santos,E.V.; Mansur,M.A.B.; Donini,I.A.N.; Zissou,V.G.; Roberto,P.G.; Marins,M. |
| The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Babesia canis vogeli; Ehrlichia canis; Ticks; RRNA gene; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200006 |
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| Velázquez-Márquez,Noé; Jiménez-Aranda,Lucio Jaime; Sánchez-Alonso,Patricia; Santos-López,Gerardo; Reyes-Leyva,Julio; Vallejo-Ruiz,Verónica. |
| Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300027 |
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| Carvalho,Rosangela Nunes; Oliveira,Antonio Nonato de; Mesquita,Albenones José de; Rezende,Cíntia Silva Minafra e; Mesquita,Adriano Queiroz de; Romero,Rolando Alfredo Mazzoni. |
| Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157®. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100002 |
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| Silva,Wladimir Padilha da; Silva,Jorge Adolfo; Macedo,Márcia Raquel Pegoraro de; Araújo,Márcia Ribeiro de; Mata,Márcia Magalhães; Gandra,Eliezer Avila. |
| Sixty-five strains of coagulase positive staphylococci (Staphylococcus aureus, S. intermedius and S. hyicus) were identified at species level by PCR amplification of the coa gene, specific for S. aureus, and of the nuc gene, specific for S. intermedius and for S. hyicus. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase positive staphylococci; PCR; Coa gene; Nuc gene. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500043 |
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| Matrone,M.; Keid,L.B.; Rocha,V.C.M.; Vejarano,M.P.; Ikuta,C.Y.; Rodriguez,C.A.R.; Ferreira,F.; Dias,R.A.; Ferreira Neto,J.S. |
| The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detection of B. abortus in clinical samples collected from aborted fetuses or calves born from cows challenged with the 2308 B. abortus strain. Therefore, two gold standard groups were built based on classical bacteriology, formed from: 32 lungs (17 positives), 26 spleens (11 positives), 23 livers (8 positives) and 22 bronchial lymph nodes (7 positives). All samples were submitted to three DNA extraction protocols, followed by the same amplification process with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Brucellosis; Abortion; PCR; DNA extraction. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010 |
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| Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa. |
| The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013 |
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| Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz. |
| Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279 |
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| Marinho,Sandra Aparecida; Teixeira,Alice Becker; Santos,Otávio Silveira; Cazanova,Ricardo Flores; Ferreira,Carlos Alexandre Sanchez; Cherubini,Karen; Oliveira,Sílvia Dias de. |
| The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgarTM Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germ-tubes, 73.7% produced chlamydoconidia, and 63.2% showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida spp; Identification; PCR; Phenotypic tests. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200004 |
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| Carvalho,Claudemir de; Freitas,Antonio Carlos de; Brunner,Olga; Góes,Luiz Gustavo Bentim; Cavalcante,Andréa Yaguiu; Beçak,Willy; Santos,Rita de Cassia Stocco dos. |
| Papillomaviruses are described selectively infecting epithelial tissues and are associated with many forms of cancer in different species. Considering the widespread dissemination of papillomatosis in livestock, interest is being centred on possible forms of viral transmission and respective mechanisms. In the present study, we report the detection of bovine papillomavirus (BPV) DNA sequences in female reproductive tract tissues, fluids and oocytes from slaughtered bovines not afflicted by cutaneous papillomatosis. BPV-2 DNA sequences were found in ovarian and uterine tissues as well as in oocytes, cumulus cells and uterine flushings. The presence of papillomavirus sequences in reproductive organ tissues and fluids shows that viral infection in organisms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BPV-2; Papillomatosis; Gametes; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500028 |
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| Ribeiro,Simone Alves Mendes; Paiva,Jaqueline Boldrin de; Zotesso,Fábio; Lemos,Manoel Victor Franco; Berchieri Júnior,Ângelo. |
| S. Pullorum (SP) and S. Gallinarum (SG) are very similar. They are the agents of pullorum disease and fowl typhoid, respectively, and the two diseases are responsible for economic losses in poultry production. Although SP and SG are difficult to be differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve this aim. While SP is able to decarboxylate this amino acid, SG is not. However, the isolation of strains showing atypical biochemical behavior has made this differentiation difficult. One of the genes associated with the metabolization of the amino acid ornithine is called speC, and is found in both serovars. The analysis of 21 SP and 15 SG strains by means of PCR did not enable... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Pullorum; Salmonella Gallinarum; Differentiation; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100032 |
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| Marchi,A. M.; Juttel,I. D.; Kawacubo,E. M.; Dalmarco,E. M.; Blatt,S. L.; Cordova,C. M. M.. |
| Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients suspected of having pulmonary tuberculosis, for whom physicians had ordered a baciloscopy test. Baciloscopy was performed using the Ziehl-Neelsen method, and culture was performed by incubation of treated samples in Lowenstein-Jensen's medium at 37ºC for eight weeks. For PCR, DNA was amplified with a specific pair of primers to the M. tuberculosis complex, with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Baciloscopy; Culture; PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400003 |
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| Gkaravela,Lamprini; Papadimitriou-Olivgeris,Matthaios; Foka,Antigoni; Kolonitsiou,Fevronia; Spiliopoulou,Anastasia; Charokopos,Nikolaos; Voulgaridis,Apostolos; Tsiamita,Maria; Marangos,Markos; Anastassiou,Evangelos D.; Spiliopoulou,Iris. |
| ABSTRACT Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus). PCR in the clinical specimens showed excellent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; PCR; GenoType MTBDRplus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400785 |
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| Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya Madureira; Timenetsky,Jorge. |
| Mycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini and M. hyorhinis, cultivated in a SP4 media. Two contamination tests were performed with BHK21 and Vero cells and one of them applied different concentrations of mycoplasma. In the first one, mycoplasma was applied at the day zero and, in the second one, the contamination was performed after the monolayer establishment. The both cellular cultures... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth reduction; PCR; Biotechnological products. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400048 |
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| Dil-Afroze,; Mir,Abdul Waheed; Kirmani,Altaf; Shakeel-ul-Rehman,; Eachkoti,Rafiqa; Siddiqi,Mushtaq A.. |
| Central nervous system (CNS) tuberculosis is a serious clinical problem, the treatment of which is sometimes hampered by delayed diagnosis. Clearly, prompt laboratory diagnosis is of vital importance as the spectrum of disease is wideand abnormalities of the cerebrospinal fluid (CSF) are incredibly variable. Since delayed hypersensitivity is the underlying immune response, bacterial load is very low. The conventional bacteriological methods rarely detect Mycobacterium tuberculosis in CSF and are of limited use in diagnosis of tuberculous meningitis (TBM). This double blind study was, therefore, directed to the molecular analysis of CNS tuberculosis by an in-house-developed PCR targeted for amplification of a 240bp nucleotidesequence coding for MPB64... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CNS tuberculosis; Tuberculous meningitis (TBM); Intracranial tuberculomas; PCR; Mycobacterium tuberculosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200002 |
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| Registros recuperados: 425 | |
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