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Registros recuperados: 30 | |
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Jiang, Lijing; Liu, Xuewen; Dong, Chunming; Huang, Zhaobin; Cambon-bonavita, Marie-anne; Alain, Karine; Gu, Li; Wang, Shasha; Shao, Zongze. |
The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. exoculata from the relatively unexplored South Mid-Atlantic Ridge. A nearly complete genome of a Deltaproteobacteria epibiont was binned from the assembled metagenome. Whole-genome phylogenetic analysis reveals that it is affiliated with the genus Desulfobulbus, representing a potential novel species for which the name "Candidatus Desulfobulbus rimicarensis" is proposed. Genomic and transcriptomic analyses reveal that this bacterium utilizes... |
Tipo: Text |
Palavras-chave: Deltaproteobacteria; Rimicaris exoculata; Wood-Ljungdahl pathway; Epibiont; Sulfur disproportionation. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73600/76007.pdf |
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Alain, Karine; Harder, Jens; Widdel, Friedrich; Zengler, Karsten. |
Aromatic hydrocarbons are among the main constituents of crude oil and represent a major fraction of biogenic hydrocarbons. Anthropogenic influences as well as biological production lead to exposure and accumulation of these toxic chemicals in the water column and sediment of marine environments. The ability to degrade these compounds in situ has been demonstrated for oxygen- and sulphate-respiring marine micro-organisms. However, if and to what extent nitrate-reducing bacteria contribute to the degradation of hydrocarbons in the marine environment and if these organisms are similar to their well-studied freshwater counterparts has not been investigated thoroughly. Here we determine the potential of marine prokaryotes from different sediments of the... |
Tipo: Text |
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Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00120/23114/21171.pdf |
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Gaboyer, Frederic; Tindall, Brian J.; Ciobanu, Maria Cristina; Duthoit, Frederique; Le Romancer, Marc; Alain, Karine. |
A novel Gram-stain-negative, strictly aerobic, heterotrophic bacterium, designated 306(T), was isolated from near-surface (109 cm below the sea floor) sediments of the Gulf of Lions, in the Mediterranean Sea. Strain 306(T) grew at temperatures between 4 and 32 degrees C (optimum 17-22 degrees C), from pH 6.5 to 9.0 (optimum 8.0-9.0) and between 0.5 and 6.0% (w/v) NaCl (optimum 2.0%). Its DNA G+C content was 58.8 mol%. On the basis of 16S rRNA gene sequence similarity, the novel isolate belongs to the class Alphaproteobacteria and is related to the genus Phaeobacter. It shares 98.7% 16S rRNA sequence identity with Phaeobacter arcticus, its closest phylogenetic relative. It contained Q-10 as the only respiratory quinone, C-18:1 omega 7c and C-16:0 as major... |
Tipo: Text |
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Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00166/27750/25981.pdf |
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Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne. |
A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex... |
Tipo: Text |
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf |
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Simon-colin, Christelle; Alain, Karine; Colin, Sebastien; Cozien, Joelle; Costa, B; Guezennec, Jean; Raguenes, Gerard. |
Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of... |
Tipo: Text |
Palavras-chave: Pseudomonas; Polyhydroxyalkanaote; Microbial mat; Kopara. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3858.pdf |
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Dalmasso, Cécile; Oger, Philippe; Courtine, Damien; Georges, Myriam; Takai, Ken; Maignien, Lois; Alain, Karine. |
We report the genome sequence of Thermococcus superprofundus strain CDGST, a new piezophilic and hyperthermophilic member of the order Thermococcales isolated from the world’s deepest hydrothermal vents, at the Mid-Cayman Rise. The genome is consistent with a heterotrophic, anaerobic, and piezophilic lifestyle. |
Tipo: Text |
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Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00345/45616/45237.pdf |
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Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne. |
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences... |
Tipo: Text |
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf |
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Aslanian, Daniel; Moulin, Maryline; Schnurle, Philippe; Klingelhoefer, Fraucke; Leroux, Estelle; Rabineau, Marina; Gailler, Audrey; Bache, Francois; Gorini, Christian; Kuroda, J.; Eguchi, N.; Droxler, Andre W.; Alain, Karine; Roure, François; Haq, B.. |
Tipo: Text |
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Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00149/26055/24160.pdf |
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Registros recuperados: 30 | |
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