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| Registros recuperados: 44 | |
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| Kouakou, Christelle; Berge, Jean-pascal; Baron, Regis; Lethuaut, Laurent; Prost, Carole; Cardinal, Mireille. |
| The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10g of D-xylose added to 1kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used, i.e. enzyme inactivation at 95°C for 30min, hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein... |
| Tipo: Text |
Palavras-chave: Salmon by-product; Hydrolysate; Maillard reaction; Sensory characteristics; Off-flavor; Soluble protein. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00156/26702/24778.pdf |
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| Dumay, Justine; Donnay-moreno, Claire; Barnathan, G; Jaouen, P; Berge, Jean-pascal. |
| Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 degrees C). After hydrolysis, three fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids and phospholipids were quantified and molecular weights of aqueous phase peptides were determined. Under these conditions, the degree of hydrolysis (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) and to 3.3% (Alcalase). Dry matter distribution indicated that all hydrolysis performed increased solubilisation of proteins: protein recovery for each hydrolysis ranged from 57.4% to 61.2%. Furthermore, the total lipid content in the liquid parts (oily and aqueous phases) increased (at least 85% of lipids... |
| Tipo: Text |
Palavras-chave: Fish protein hydrolysate FPH; Sardine; By product; Lipid; Hydrolysis; Phospholipid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2004.pdf |
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| Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella. |
| Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show... |
| Tipo: Text |
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| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf |
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| Bourseau, Patrick; Chabeaud, Aurélie; Vandanjon, Laurent; Masse, Anthony; Jaouen, Pascal; Fleurence, Joël; Berge, Jean-pascal. |
| This review reports some interesting works on the recovery and fractionation by membrane techniques of marine compounds resulting from hydrolysis of fish by-products such as proteins or peptides, lipids or fatty acids, and carbohydrates. Ultrafiltration (UF) appears as the most widely used technique for such applications. It must be noted that only a few works deal with the optimization or the enhancement of processes for the recovery of added value molecules. Indeed, most of works use UF as a convenient and simple tool for solute fractionation, for this reason the influence of operating parameters on membrane separation efficiency is unfortunately seldom considered. When attention is paid in a study to the process development, it is specified in this... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7408.pdf |
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| Kechaou, Emna; Durnay, Justine; Donnay-moreno, Claire; Jaouen, Pascal; Gouygou, Jean-paul; Berge, Jean-pascal; Ben Arnar, Raja. |
| Total lipid and phospholipid recovery as well as amino acid quality and composition from cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) were compared. Enzymatic hydrolyses were performed using the three proteases Protamex, Alcalase, and Flavourzyme by the pH-stat method (24 h, pH 8, 50 degrees C). Three fractions were generated: an insoluble sludge, a soluble aqueous phase, and an oily phase. For each fraction, lipids, phospholipids, and proteins were quantified. Quantitative and qualitative analyses of the raw material and hydrolysates were performed. The degree of hydrolysis (DH) for cuttlefish viscera was 3.2% using Protamex, 6.8% using Flavourzyme, and 7% using Alcalase. DH for sardine viscera was 1.9% (using Flavourzyme), 3.1% (using... |
| Tipo: Text |
Palavras-chave: Sardine; Viscera; Proteolysis; Marine lipids; Cuttlefish; By products. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6366.pdf |
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| Picot, Laurent; Ravallec, Rozenn; Fouchereau-peron, Martine; Vandanjon, Laurent; Jaouen, Pascal; Chaplain-derouiniot, Maryse; Guerard, Fabienne; Chabeaud, Aurelie; Legal, Yves; Martinez Alvarez, Oscar; Berge, Jean-pascal; Piot, Jean-marie; Batista, Irineu; Pires, Carla; Thorkelsson, Gudjon; Delannoy, Charles; Jakobsen, Greta; Johansson, Inger; Bourseau, Patrick. |
| BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing... |
| Tipo: Text |
Palavras-chave: Fish protein hydrolysate; Ultrafiltration; Nanofiltration; Membrane separation; Fractionation process; Bioactive peptide. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12217/9259.pdf |
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| Sylla, K. S. B.; Musabyemariya, B.; Berge, Jean-pascal; Seydi, Mg. |
| The by-products (viscera, heads, backbone and skins) of tongue sole coming from a Senegal frozen fisherie plant, were hydrolized with the Protamex® enzyme.Degrees of hydrolysis of 30 and 19% were obtained respectively with 40°C and 50°C.The results also showed that the proteins contained in these by-products are completely solubilized by the action of this protease at the end of 180 minutes of hydrolysis. The biochemical characterization showed that these hydrolysates are rich in proteins (61%) and minerals (10%). However, the hydrolysates contain peptides of lower molecular sizes 1760Da.Lastly, these hydrolysates have high nutritional value in order to set up table fowls because they have in big quantity 9 essential amino acids for the poultry feed. |
| Tipo: Text |
Palavras-chave: Co-produits; Hydrolyse enzymatique; Protéines; By-products; Enzymatic hydrolysis; Proteins. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00015/12621/9506.pdf |
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| Randriamahatody, Zo; Sylla, K. S. B.; Nguyen, Thi-my-huong; Donnay-moreno, Claire; Razanamparany, L.; Bourgougnon, N.; Berge, Jean-pascal. |
| To generate and recover elements from shrimp heads, proteolysis was performed with commercial acidic or alkaline proteases for 22 h. The resulting phases were characterized for protein and lipid content and amino acid composition while the molecular profiles of soluble peptides were established. Whatever the protease used, more than the half of the initial dry matter was found into aqueous phase including most of the proteins while lipids were found to remain insoluble. Hydrolysates were mainly constituted by small peptides (480% below 1000 Da) with up to 14–15 amino acids identified. Moreover, all the hydrolyses have led to an increase of the amount of essential amino acids into the hydrolysates, including lysine. Thus, such proteolysis of shrimp heads can... |
| Tipo: Text |
Palavras-chave: By-products; Shrimp; Proteolysis; Hydrolysate. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00055/16646/14196.pdf |
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| Fargeas, Valérie; Baalouch, Myriam; Metay, Estelle; Baffreau, Jerôme; Menard, Dominique; Gosselin, Pascal; Berge, Jean-pascal; Barthomeuf, Chantal; Lebreton, Jacques. |
| A simple and efficient methodology to introduce an 1,3-diketone motif from various aldehyde precursors in three steps with good overall yields is described using beta-ketosulphone 7 as masked equivalent of acetone. |
| Tipo: Text |
Palavras-chave: 1; 3 diketones Alkylation Sulfones Aldehydes. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-829.pdf |
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| Berge, Jean-pascal. |
| La présence de substances immunologiquement apparentées aux O/CCK de vertébrés est détectée chez les crustacés décapodes (Favrel,1988; Turrigiano G.G,1989). Un protocole de purification est même établi pour Palaemon serratus et Nephrops norvegicus (Favrel,1988). Dans un premier temps, nous allons nous attacher à la purification des G/CCK immunologiquement apparentées aux hormones peptidiques de vertébrés (O/CCK"like") chez les deux espèces précitées. Dans un deuxième temps, à l'aide des fractions purifiées, le rôle biologique des G/CCK"Iike" et particulièrement leur action sur la synthèse protéique vont être étudiés. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00136/24708/22762.pdf |
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| Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja. |
| Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order.... |
| Tipo: Text |
Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf |
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| Baron, Regis; Socol, Marius; Arhaliass, A.; Bruzac, Sandrine; Le Roux, Karine; Del Pino, J. Rodriguez; Berge, Jean-pascal; Kaas, Raymond. |
| We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99 % of demineralization yield... |
| Tipo: Text |
Palavras-chave: Bio-refinery; Acidic enzymatic proteolysis; Shrimp cuticles; Demineralization kinetics and modeling. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00281/39244/37809.pdf |
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| Dubois, Nolwenn; Barnathan, Gilles; Gouygou, Jean-paul; Berge, Jean-pascal. |
| The gas chromatographic properties of four derivatives of fatty acids (FA), namely fatty acid methyl esters, picolinyl esters, N-acyl pyrrolidides and 4,4-dimethyloxazoline derivatives, which contain various structural features (double bonds, branching, hydroxyl group) in their acyl chains have been compared on a low-polarity capillary column with a mass spectrometer as detector. Temperature programming rates yielding the highest resolution were optimized for each derivative by means of computer-assisted column temperature optimization software. Indeed, the Drylab software represents a valuable assistance for estimating the optimum analysis conditions. Time and efforts required for such method development can greatly be reduced. Different parameters... |
| Tipo: Text |
Palavras-chave: Picolinyl esters N Acyl pyrrolidides Methyl esters GC MS Fatty acid derivatives 4; 4 Dimethyloxazoline. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6846.pdf |
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| Perez-galvez, Raul; Chopin, Christine; Mastail, Max; Ragon, Jean-yves; Guadix, Antonio; Berge, Jean-pascal. |
| Fresh sardine (employed as discard model species) was pressed with a hydraulic press in order to obtain a partially dewatered cake (whose volume represents between 55% and 60% of the initial Volume of raw material) and a 3-phase press liquor comprising an aqueous fraction, an oil fraction and a variable amount of suspended solids. Pressing conditions, such as final pressure to which the sardine was subjected, compression speed, number of pressing stages and time of relaxation between two consecutive pressing stages, were optimised by means of a statistically designed experiment. The goal of the optimisation problem was to achieve maximal yield of press liquor with a minimum load of suspended solids. The Suspended solid content in the press liquor Must be... |
| Tipo: Text |
Palavras-chave: Multiobjective optimisation; Response surface methodology; Hydraulic pressing; Fish discards; Sardine. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7415.pdf |
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| Bazes, Alexandra; Silkina, Alla; Douzenel, Philippe; Fay, Fabienne; Kervarec, Nelly; Morin, Dimitri; Berge, Jean-pascal; Bourgougnon, Nathalie. |
| One of the most promising alternatives to toxic heavy metal-based paints is offered by the development of antifouling coatings in which the active ingredients are compounds naturally occurring in marine organisms and operating as natural antisettlement agents. Sessile marine macroalgae are remarkably free from settlement by fouling organisms. They produce a wide variety of chemically active metabolites in their surroundings, potentially as an aid to protect themselves against other settling organisms. In this study, a dichloromethane extract from the brown seaweed Sargassum muticum was tested in situ and, after 2 months of immersion, showed less fouling organisms on paints in which the extract was included, compared to paints containing only copper after 2... |
| Tipo: Text |
Palavras-chave: Sargassum muticum; Palmitic acid; Dioctyl phthalate; Dichloromethane extract; Brown algae; Antifouling. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6601.pdf |
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| Ruiz, Nicolas; Dubois, Nolwenn; Wielgosz Collin, Gaetane; Du Pont, Thibaut; Berge, Jean-pascal; Pouchus, Yves-francois; Barnathan, Gilles. |
| A marine-related Trichoderma longibrachiatum strain isolated from mussels in a farming shellfish area was investigated for total lipid (TL) production, total lipid fatty acids (TLFA), and phospholipid fatty acids (PLFA). Fungal biomass was produced from two different marine-like culture ways, on agar surface and submerged fermentations (ASF and SmF, respectively), allowing useful comparisons. ASF produced a rather higher biomass amount than SmF with similar TL content. All fatty acids (FA), identified by gas chromatography-mass spectrometry (GC-MS), were ranged from 14 to 30 carbon atoms. Similar FA compositions were found in TL and phospholipids (PL) from biomasses obtained by both fermentation systems, including oleic (up to 15.3% of total FA mixture for... |
| Tipo: Text |
Palavras-chave: Conjugated fatty acids; Fatty acids; Lipids; Submerged fermentation; Agar surface fermentation; Trichoderma longibrachiatum. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4199.pdf |
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| Registros recuperados: 44 | |
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