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| Registros recuperados: 35 | |
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| Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P.. |
| Rationale for EST and genome sequencing of the Pacific oyster: its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), its high fecundity, with concomitantly high DNA polymorphism, its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST... |
| Tipo: Text |
Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf |
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| Chong-robles, Jennyfers; Charmantier, Guy; Boulo, Viviane; Lizarraga-valdez, Joel; Enriquez-paredes, Luis M.; Giffard-mena, Ivone. |
| The euryhaline white shrimp Litopenaeus vannamei lives in both coastal and oceanic areas through ontogeny. Its osmoregulation pattern and variations in its tolerance to salinity are partially known from several studies under different experimental conditions (developmental stages, salinities and acclimation procedures). Although L. vannamei is recognized as one of the most euryhaline penaeid species, with adults and juveniles exhibiting an hyper-hypo-osmoregulatory pattern and being able to tolerate a wide salinity range, little is known on larval and early postlarval strategies to cope with salinity fluctuations. In order to establish their euryhalinity range and to fully understand the ontogenetic changes in L. vannamei osmoregulatory pattern, we... |
| Tipo: Text |
Palavras-chave: Osmotic pressure; Hypo-hyper osmoregulation; Osmotic stress; Survival; Crustacean; Larvae. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28068/26283.pdf |
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| Labreuche, Yannick; Lambert, Christophe; Soudant, Philippe; Boulo, Viviane; Huvet, Arnaud; Nicolas, Jean-louis. |
| The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Pathogenesis; Vibrio aestuarianus; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2182.pdf |
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| Bossus, Maryline; Charmantier, Guy; Blondeau-bidet, Eva; Valletta, Bianca; Boulo, Viviane; Lorin-nebel, Catherine. |
| Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW,... |
| Tipo: Text |
Palavras-chave: ClC-3 chloride channel; Na+/K+-ATPase; Dicentrarchus labrax; Osmoregulation; Osmosensing. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00149/26044/25185.pdf |
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| Giffard Mena, I; Lorin Nebel, C; Charmantier, G; Castille, R; Boulo, Viviane. |
| Sea-bass (Dicentrarchus labrax) grow under different salinity regimes, from the open sea to lagoons and even rivers, but some mortality has been recorded in juvenile stages when exposed to low salinity water. Changes in water permeability of different osmoregulatory tissues could be the cause of reduction in blood osmotic pressure and death in some fish in fresh water (FW). In order to explore this condition, we have studied the changes of aquaporins (AQP1 and AQP3), alpha 1 and alpha 4 Na+/K(+-)ATPase transcript levels in the digestive tract, kidney and gills after a long-term exposure of juvenile sea-bass to sea water (SW) and FW fish able to survive in SW and FW are called SW-adapted fish (SWS), FW successfully-adapted fish (FWS) respectively, while... |
| Tipo: Text |
Palavras-chave: Salinity adaptation; Juvenile sea bass; Differential mortality; Alpha 4 Na+/K+ ATPase; Alpha l Na+/K+ ATPase; AQP3; AQP1. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4656.pdf |
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| Lemonnier, Hugues; Barri, Kiam; Pham, Dominique; Wabete, Nelly; Boulo, Viviane; Lignot, Jean-herve; Royer, Florence; Hubert, Morgane; Blockmans, Bernard; Pierrot, Thomas; Laugier, Thierry; Tostin, Nathalie. |
| L'aquaculture est une activité importante du paysage économique de la Nouvelle-Calédonie., La crevette Bleue du Pacifique, Litopenaeus stylirostris est aujourd’hui l’espèce cible, élevée en semi-intensif, dans des bassins de terre de 4 à 10 hectares. Une coloration orange prononcée au niveau des branchies, comparable à celle observée suite à un stress hypoxique alors qu’aucun déficit en oxygène n’est constaté, est apparue récemment engendrant une décote du produit à la vente et donc un risque économique supplémentaire pour cette filière déjà fragilisée par des mortalités à vibrioses. Le phénomène se déclare à un poids moyen des animaux compris entre 10 et 18 g pour une durée moyenne d’élevage de 70 jours. L'intensité de la couleur évolue avec le stade de... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00248/35909/34462.pdf |
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| Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge. |
| The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and... |
| Tipo: Text |
Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf |
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| Noel, Danielle; Boulo, Viviane; Chagot, Dominique; Mialhe, Eric; Paolucci, Francis; Clavies, Christine; Hervaud, Eric; Elston, Ralph. |
| Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2757.pdf |
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| Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G. |
| This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after... |
| Tipo: Text |
Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf |
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| Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane. |
| The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval... |
| Tipo: Text |
Palavras-chave: Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2612.pdf |
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| Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. |
| The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... |
| Tipo: Text |
Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf |
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| Registros recuperados: 35 | |
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