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Registros recuperados: 62 | |
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Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah. |
Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg... |
Tipo: Text |
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf |
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Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier. |
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All... |
Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology. |
Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf |
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Hylland, Ketil; Skei, Bjorn Borge; Brunborg, Gunnar; Lang, Thomas; Gubbins, Matthew J.; Le Goff, Jeremie; Burgeot, Thierry. |
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and... |
Tipo: Text |
Palavras-chave: ICON; Comet; DNA adducts; Genotoxicity; Haddock; Dab; European seas. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00309/42064/44971.pdf |
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Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean. |
Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All... |
Tipo: Text |
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Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf |
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Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre. |
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials... |
Tipo: Text |
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf |
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Barranger, Audrey; Benabdelmouna, Abdellah; Degremont, Lionel; Burgeot, Thierry; Akcha, Farida. |
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in... |
Tipo: Text |
Palavras-chave: Aneuploidy; FISH; Diuron; Crassostrea gigas. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00238/34911/33216.pdf |
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Sanchez, Wilfried; Burgeot, Thierry; Porcher, Jean-marc. |
Multi-biomarker approaches are used to assess ecosystem health and identify impacts of environmental stress on organisms. However, exploration of large datasets by environmental managers represents a major challenge for regulatory application of this tool. Several integrative tools were developed to summarize biomarker responses. The aim of the present paper is to update calculation of the "Integrated Biological Response" (IBR) described by Beliaeff and Burgeot (Environ Toxicol Chem 21:1316-1322, 2002) to avoid weaknesses of this integrative tool. In the present paper, a novel index named "Integrated Biological Responses version 2" based on the reference deviation concept is presented. It allows a clear discrimination of sampling sites as for the IBR, but... |
Tipo: Text |
Palavras-chave: Biomarkers; Data analysis; Graphic tools; Three-spined stickleback. |
Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00139/25069/23191.pdf |
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Huvet, Arnaud; Royer, J.; Moal, J.; Burgeot, Thierry; Lapègue, Sylvie; Boulo, Viviane; Nicolas, Jean-louis; Lambert, C.. |
Les résultats du programme MOREST démontrent l'existence d'une composante génétique à la survie estivale du naissain et nous permettent de disposer aujourd'hui d'huTtres « résistantes» (R) et « susceptibles» (S) aux mortalités estivales (Dégremont et aL, 2002, 2005). La forte héritabilité estimée face à un tel système multifactoriel permet de suspecter que le caractère sélectionné puisse être gouverné par un ou un trés petit nombre de gènes sur une région chromosomique donnée. D'après le modèle d'interaction suspecté entre l'environnement, l'huître et des pathogènes opportunistes, une hiérarchie des facteurs, conduisant aux mortalités, permet de montrer qu'aucun d'entre eux ne peut provoquer à lui seul les mortalités mais que leur combinaison est... |
Tipo: Text |
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Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00306/41706/40917.pdf |
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Mauffret, Aourell; Chiffoleau, Jean-francois; Burgeot, Thierry; Wessel, Nathalie; Brun, Melanie. |
Good Environmental Status (GES) for descriptor 8 of MSFD is reached when concentrations of contaminants are at levels not giving rise to pollution effects. According to the decision 2017/848/CE, descriptor 8 is defined by four criteria: concentration in the relevant matrices (sediment, biota) (D8C1), effects of chronic contamination to the marine organisms (D8C2), duration and spatial extent of acute pollution events (D8C3), effects of acute contamination to the marine organisms (D8C4). The GES for descriptor 8 is assessed at the level of four marine subregions (SRM), including: Channel – North Sea (MMN), Celtics Seas (MC), Bay of Biscay (GdG) and the Occidental Mediterranean Sea (MO). In order to achieve GES, each indicator of descriptor 8 should respect... |
Tipo: Text |
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Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57294/59374.pdf |
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Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil. |
The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons... |
Tipo: Text |
Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf |
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Akcha, Farida; Burgeot, Thierry; Budzinski, H; Pfohla Leszkowicz, A; Narbonne, J. |
With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg(-1) dry wt mussel d(-1)) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was... |
Tipo: Text |
Palavras-chave: DNA repair; 8 oxodGuo; DNA adducts; Mussel; BaP. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-813.pdf |
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Poisson, Emeline; Fisson, Cédric; Amiard-triquet, Claude; Burgeot, Thierry; Couteau, Jérôme; Dur, Gael; Durand, Fabrice; Forget-leray, Joelle; Letendre, Julie; Souissi, Sami; Xuereb, Benoit. |
Le présent fascicule a pour objectif d’apporter les éléments de réponse disponibles pour l’estuaire de la Seine sur ce questionnement. Dans la première partie écotoxicologie. Les effets de la contamination chimique à différents niveaux d’organisation biologique, sur les organismes présents dans l’estuaire de la Seine, seront présentés dans la deuxième partie puis synthétisés dans notion d’analyse du risque environnemental. |
Tipo: Text |
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Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00319/43019/42534.pdf |
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Simon, Alexis; Arbiol, Christine; Nielsen, Einar Eg; Couteau, Jérôme; Sussarellu, Rossana; Burgeot, Thierry; Bernard, Ismaël; Coolen, Joop W. P.; Lamy, Jean-baptiste; Robert, Stephane; Skazina, Maria; Strelkov, Petr; Queiroga, Henrique; Cancio, Ibon; Welch, John J.; Viard, Frédérique; Bierne, Nicolas. |
Human‐mediated transport creates secondary contacts between genetically differentiated lineages, bringing new opportunities for gene exchange. When similar introductions occur in different places, they provide informally replicated experiments for studying hybridisation. We here examined 4,279 Mytilus mussels, sampled in Europe and genotyped with 77 ancestry‐informative markers. We identified a type of introduced mussels, called “dock mussels,” associated with port habitats and displaying a particular genetic signal of admixture between M. edulis and the Mediterranean lineage of M. galloprovincialis. These mussels exhibit similarities in their ancestry compositions, regardless of the local native genetic backgrounds and the distance separating colonised... |
Tipo: Text |
Palavras-chave: Admixture; Bentho-pelagic species; Biological introductions; Clines; Ports; Secondary contact. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70600/68791.pdf |
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Hylland, Ketil; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Robinson, Craig D.; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Matthew J.. |
An international workshop on marine integrated contaminant monitoring (ICON) was organised to test a framework on integrated environmental assessment and simultaneously assess the status of selected European marine areas. Biota and sediment were sampled in selected estuarine, inshore and offshore locations encompassing marine habitats from Iceland to the Spanish Mediterranean. The outcome of the ICON project is reported in this special issue as method-oriented papers addressing chemical analyses, PAH metabolites, oxidative stress, biotransformation, lysosomal membrane stability, genotoxicity, disease in fish, and sediment assessment, as well as papers assessing specific areas. This paper provides a background and introduction to the ICON project, by... |
Tipo: Text |
Palavras-chave: ICON; Contaminants; North sea; Mediterranean sea; Biological effects; Biomarkers; Monitoring. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00297/40782/44970.pdf |
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Parmentier, Koen F. V.; Verhaegen, Yves; De Witte, Bavo P.; Hoffman, Stefan; Delbare, Daan H. R.; Roose, Patrick M.; Hylland, Ketil D. E.; Burgeot, Thierry; Smagghe, Guy J.; Cooreman, Kris. |
The restrictions and the concerted action of the global ban on the use and presence of tributyltin (TBT) in marine applications to protect ecosystems in the marine environment in 2008 was mainly based on the economic impact on shellfish industries and the dramatic extinction of local mollusk populations in the past. In contrast to the vast datasets on effects on mollusks, the knowledge on impacts on species from other taxa remained in the uncertain until almost two decades ago. The assumption on a long-term TBT-mediated pernicious metabolic bottom–up regulation of the crustacean Crangon crangon population was provoked by the outcome of an EU-project ‘Sources, Consumer Exposure and Risks of Organotin Contamination in Seafood.’ This study reported high TBT... |
Tipo: Text |
Palavras-chave: Tributyltin; Crangon crangon; Endocrine disruption; Ecosystem impact; Ecdysteroids; Ecdysteroid receptor. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00585/69727/67617.pdf |
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Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F.. |
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was... |
Tipo: Text |
Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf |
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Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in... |
Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf |
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Registros recuperados: 62 | |
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