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| Registros recuperados: 34 | |
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| Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie. |
| The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf |
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| Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie. |
| The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end... |
| Tipo: Text |
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf |
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| Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre. |
| La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf |
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| Cornette, Florence. |
| L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR... |
| Tipo: Text |
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf |
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| Marandel, Florianne; Biais, Gerard; Bidault, Adeline; Charrier, Grégory; Cornette, Florence; Lapegue, Sylvie; Laroche, Jean; Le Cam, Sabrina; Stephan, Eric; Trenkel, Verena; Lorance, Pascal. |
| At the ecosystem level, sustainable exploitation of fisheries resources depends not only on the status of target species but also on that of bycatch species, some of which are even more sensitive to exploitation. This is the case for a number of elasmobranchs (skates, rays and sharks) species whose abundance declined during the 20th century. Further, the biology of elamobranchs is still poorly known and traditional fisheries stock assessment methods using fisheries catches and scientific survey data for estimating abundance are expensive or even inapplicable due to the small numbers observed. The GenoPopTaille project attempts to apply to the case of the thornback ray (Raja clavata) recent genetic-based methods for absolute population abundance estimation... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00343/45439/44960.pdf |
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| Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre. |
| Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program... |
| Tipo: Text |
Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf |
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| Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie. |
| es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)... |
| Tipo: Text |
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois. |
| Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each... |
| Tipo: Text |
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf |
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| Cornette, Florence; Blin, J.l.; Pacary, S; Pien, S; Le Gagneur, Eric; Kopp, Joel; Richard, O. |
| At the end of its third year, the regional network for monitoring REMONOR, confirms the particularities of oyster from Normandy in matter of growth and quality. Results from 2 000 are globally very close of those from 1998. Mortality of adults reached 14% and juvenile 6% which represent a low death rate. For this year the most blow area is the Baie des Veys with 31% death rate at Géfosse. Observed growth this year had been weaker than the past two years despite the spring and automnal growth. At spring, the higher recorded growth rate on the West coast with 0.42% (regional average : 0.29%). This seasonal trend seems more and more obvious every year. Regarding the classification of the special oysters, despite the change of the standard, the Baie des Veys... |
| Tipo: Text |
Palavras-chave: REMORA; Quality; Growth; Mortality; Normandy; REMONOR; Crassostrea gigas; REMORA; Qualité; Croissance; Mortalité; Basse Normandie; REMONOR; Crassostrea gigas. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/rapport-2251.pdf |
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| Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean. |
| We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. |
| Tipo: Text |
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf |
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| Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre. |
| Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids bred in this species. Tetraploid animals with lower chromosome counts in some or all of their somatic tissue are considered undesirable as parents either for commercial triploids (produced via a cross with diploids) or for tetraploid breeding because (1) they may confer the tendency to lose chromosomes and revert to lower ploidy levels to their offspring or (2) their offspring could have a lower ploidy directly because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in bi-parental tetraploid families bred from parents of differing ploidy quality, including a... |
| Tipo: Text |
Palavras-chave: Tetraploid; Chromosome; Genetic; Crassostrea gigas; Oyster. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3435.pdf |
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| Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max. |
| The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae. |
| Tipo: Text |
Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf |
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| Registros recuperados: 34 | |
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