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Registros recuperados: 35
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Do river discharge rates drive the overall functioning of the pelagic ecosystem over the continental shelf of the Bay of Biscay (NE Atlantic)? A comparison of two contrasting years with special reference to anchovy (Engraulis encrasicolus L.) nutritional state ArchiMer
Bergeron, Jean-pierre; Delmas, Daniel; Koueta, Noussithe.
In the 2000 and 2001 springtime periods, mesozooplankton samples were systematically taken during research cruises devoted to the study of the distribution and abundance of small pelagic fishes in the French part of the Bay of Biscay, NE Atlantic, continental shelf. Simultaneous activities of three enzymes were measured in these mesozooplankton samples, to characterize the main features of ecosystem function at a regional scale. The aim was to assess assimilation rates of carbohydrates, through pyruvate kinase activity, and protein, through trypsin activity, by the mesozooplankton community and the resulting overall productivity, through aspartate transcarbamylase activity. The highest influence on the pelagic environment was the strong difference in the...
Tipo: Text Palavras-chave: Rivers discharge; Phytoplankton distribution; Anchovy; Nutritional state; RNA/DNA ratio; Mesozooplankton; Metabolic descriptors; North-East Atlantic; Bay of Biscay.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00012/12290/9089.pdf
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Etude chémotaxonomique de la campagne TIC-MOC (mars 2015) – Projet CALHIS ArchiMer
Lampert, Luis; Perrot, Laurie; Delmas, Daniel.
A partir des échantillons collectés lors de la campagne TIC-MOC (mars 2015) sur le plateau continental argentin, des analyses en pigments phytoplanctoniques permettent de quantifier la distribution des principales classes algales par la méthode CHEMTAX. Les Prochlorophycées et cyanobactéries sont essentiellement dominantes dans les eaux chaudes du courant du Brésil, tandis que dans les eaux plus froides du courant des Malouines et la zone de mélange (T°< 20°C), c’est les Haptophytes (Phaeocystis et Emiliania huxleyi) qui dominent la biomasse chlorophyllienne. Des dominances moyennes en dinoflagellés supérieures 30% sont observées dans les deux masses d’eau.
Tipo: Text Palavras-chave: Chémotaxonomie; Coccolithophoridés; HPLC; Pigments phytoplanctoniques; Patagonie; Plateau continental.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00327/43784/43342.pdf
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High alkaline phosphatase activity in phosphate replete waters: The case of two macrotidal estuaries ArchiMer
Labry, Claire; Delmas, Daniel; Youenou, Agnes; Quere, Julien; Leynaert, Aude; Fraisse, Stephane; Raimonet, Melanie; Ragueneau, Olivier.
The occurrence of alkaline phosphatase activity (APA) that hydrolyses organic phosphorus into phosphate (PO4) is commonly related to PO4 deficiency of oceanic, coastal and fresh waters. APA is almost never investigated in PO4-rich estuaries, since very low activities are expected to occur. As a consequence, microbial mineralization of organic phosphorus into PO4 has often been ignored in estuaries. In this study, we examined the importance of potential APA and the associated microbial dynamics in two estuaries, the Aulne and the Elorn (Northwestern France), presenting two different levels of PO4 concentrations. Unexpected high potential APA was observed in both estuaries. Values ranged from 50 to 506 nmol L−1 h−1, which range is usually found in very...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00337/44840/44428.pdf
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Potential Effect of Freshwater Virus on the Structure and Activity of Bacterial Communities in the Marennes-Oléron Bay (France) ArchiMer
Auguet, Jean-christophe; Montanie, Helene; Hartmann, Hans; Lebaron, P.; Casamayor, E. O.; Catala, P.; Delmas, Daniel.
Batch culture experiments using viral enrichment were conducted to test the response of a coastal bacterial community to autochthonous (i.e., co-existing) or allochthonous riverine viruses. The effects of viral infections on bacterial dynamics and activity were assessed by epifluorescence microscopy and thymidine incorporation, respectively, whereas the effect of viral infection on bacterial community composition was examined by polymerase chain reaction-single strand conformation polymorphism 16S ribosomal RNA fingerprinting. The percentages of high nucleic acid-containing cells, evaluated by flow cytometry, were significantly correlated (r (2) = 0.91, n = 12, p < 0.0001) to bacterial production, making this value a good predictor of active cell...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6636.pdf
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Effect of P depletion on the functional pools of diatom carbohydrates, and their utilization by bacterial communities ArchiMer
Labry, Claire; Delmas, Daniel; Moriceau, Brivaela; Gallinari, Morgane; Quere, Julien; Youenou, Agnes.
Phosphorus (P) limitation of phytoplankton growth is known to affect the accumulation and release of carbohydrates (CHO) by micro-algae. However, relatively little is known about the fate of algal exudates, notably their bacterial degradation. The CHO chemical characterization is also not exhaustive, especially in ‘functional’ pools relevant for phytoplankton physiology (particulate reserve [R] or structural [S] CHO) and for bacterial degradation (dissolved mono- [MDCHO] and polysaccharides [P-DCHO]). In this study, we investigated how P depletion and repletion affect the CHO composition in diatom Thalassiosira weissflogii cultures, and the shortterm response of free and diatom-attached bacteria in terms of abundance and potential βglucosidase activity...
Tipo: Text Palavras-chave: P limitation; Phytoplankton; Diatom; Bacteria; Mineralization; Carbohydrate; Glucosidase.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00628/74045/73532.pdf
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The role of phosphorus on planktonic production of the Gironde plume waters in the Bay of Biscay ArchiMer
Labry, Claire; Herbland, Alain; Delmas, Daniel.
More and more studies emphasize the status of phosphorus (P) as the principal limiting nutrient of phytoplankton growth, especially in coastal waters under the influence of freshwater discharges. The purpose of the present paper is to investigate the role of P on planktonic production in the waters influenced by the Gironde discharges; the Gironde being one of the two largest rivers on the French Atlantic coast. The survey is based on several cruises made in 1998 and 1999. Two different patterns were observed for waters with salinity below and above 34.5. For waters with salinity <34.5. P was found to be the first limiting nutrient of winter and spring phytoplankton blooms, based on undetectable phosphate (<20 nM), high NO3 : PO4 ratios, typically...
Tipo: Text Palavras-chave: Bay of Biscay; River plume; Salinity; Water quality analysis; Phytoplankton; Planktonic production.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-690.pdf
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Microbial enzymatic assays in environmental water samples: impact of Inner Filter Effect and substrate concentrations ArchiMer
Urvoy, Marion; Labry, Claire; Delmas, Daniel; Creac'H, Layla; L Helguen, Stephane.
As microbial enzymatic activities initiate the mineralization of organic matter through the microbial loop, it is important to correctly measure those activities and be able to perform inter-study comparisons. Enzymatic activity assays are typically carried out using fluorogenic substrate analogs, such as 4-Methylumbelliferone and 7-Amino-4-methylcoumarin linked to sugar monomers, phosphate group or amino acids. However, methodological divergences can be found in aquatic science literature, potentially leading to misestimated activities. To highlight some of those methodological key points, we first addressed the potential occurrence of an Inner Filter Effect (IFE), a fluorometric artifact that affects the relationship between fluorophore concentration and...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00653/76524/77614.pdf
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Seasonal variation of riverine nutrient inputs in the northern Bay of Biscay (France), and patterns of marine phytoplankton response ArchiMer
Guillaud, Jean-francois; Aminot, Alain; Delmas, Daniel; Gohin, Francis; Lunven, Michel; Labry, Claire; Herbland, Alain.
Seasonal variations in nutrient inputs are described for the main rivers (Loire and Vilaine) flowing into the northern Bay of Biscay. The river plumes are high in N/P ratio in late winter and spring, but not in the inner plume during the summer. Conservative behavior results in most nutrients entering the estuary and eventually reaching the coastal zone. Temporal and spatial aspects of phytoplankton growth and nutrient uptake in the northern Bay of Biscay distinguish the central area of salinity 34 from the plume area. The first diatom bloom appears offshore in late winter, at the edge of the river plumes, taking advantage of haline stratification and anticyclonic "weather windows." In spring, when the central area of the northern shelf is...
Tipo: Text Palavras-chave: Northern Bay of Biscay; Phytoplankton; Continental shelf; Riverine inputs; Nutrients.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2007/publication-2354.pdf
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Nitrogenous nutrient transfers in oyster ponds role of sediment in deferred primary production ArchiMer
Sornin, Jean-marc; Collos, Yves; Delmas, Daniel; Feuillet-girard, Michèle; Gouleau, Daniel.
The oyster pond under study has a natural sediment bottom and water is changed only once or twice every month depending on tidal height. Between successive water renewals, the pond is a closed system, equivalent to a batch culture in which sinking and biodeposition of particulate matter are the only sources of organic matter to the bottom. Particulate organic matter enrichment of the sediment in winter is followed by intensive ammonification in summer with very limited nitrification. Seasonal nitrogen budgets involving particulate and dissolved phases show that in summer during batch mode period, ammonium enrichment from the sediment to the overlying water is an order of magnitude higher than the initial nutrient supply in the feed water. This allows a...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00178/28892/27749.pdf
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In situ structuring of virioplankton through bacterial exoenzymatic activity: interaction with phytoplankton ArchiMer
Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene.
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using...
Tipo: Text Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf
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Phytoplankton spring bloom of the Gironde plume waters in the Bay of Biscay: early phosphorus limitation and food-web consequences ArchiMer
Herbland, Alain; Delmas, Daniel; Laborde, P; Sautour, B; Artigas, F.
During the spring 1995 (2-25 May), a cruise was carried on the RV Poseidon (Germany) on the continental shelf of the south Bay of Biscay. The objective was a comprehensive study of the planktonic food web within the Gironde plume waters. In these waters phosphate was present at very low concentrations (undetectable to < 0.1 mu mol.L-1), whereas nitrate, silicate and ammonium concentrations were much higher (several mu mol.L-1 for nitrate and silicate and 0.5 to 1.0 mu mol.L-1 for ammonium). The size distribution of the phytoplankton biomass (estimated from chlorophyll a measurements by high performance liquid chromatography) and primary production (measured by C-14 in situ method) showed a great proportion of small (40 to 70 % < 3 mu m) and active...
Tipo: Text Palavras-chave: Phosphate; Phytoplancton; Facteur limitant; Estuaire; Eau côtière; Phosphate; Phytoplankton; Limiting factor; Estuarine water; Coastal water.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-850.pdf
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Initiation of winter phytoplankton blooms within the Gironde plume waters in the Bay of Biscay ArchiMer
Labry, Claire; Herbland, Alain; Delmas, Daniel; Laborde, P; Lazure, Pascal; Froidefond, J; Jegou, Anne-marie; Sautour, B.
Thermostratification and seasonal Light increase are generally considered the first causes of phytoplankton spring blooms in temperate waters. The objective of this study is to confirm the existence of winter phytoplankton blooms, responsible for the early exhaustion of phosphate, within the Gironde plume waters (southeast Bay of Biscay), and to understand what may initiate them so early. Two cruises, BIOMET 2 and BIOMET 3, were carried out respectively in early (8 to 21 January) and late winter 1998 (25 February to 11 March). An increase of phytoplankton biomass (chl a) between the 2 cruises and non-conservative nutrients observed in late winter confirm an early phytoplankton growth both in the Gironde plume and adjacent oceanic waters. Among factors that...
Tipo: Text Palavras-chave: Vertical attenuation coefficient; Halostratification; Light availability; Winter blooms.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-810.pdf
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Use of SeaWiFS data for light availability and parameter estimation of a phytoplankton production model of the Bay of Biscay ArchiMer
Huret, Martin; Gohin, Francis; Delmas, Daniel; Lunven, Michel; Garcon, Véronique.
Processing SeaWiFS (Sea-viewing Wide Field-of-view Sensor) data provides useful information for the observation and modelling of the phytoplankton production of the Bay of Biscay. Empirical algorithms allow the retrieval of chlorophyll a and non-living Suspended Particulate Matter (SPM) concentrations. These data are used to constrain a coupled 3D physical-biogeochemical model of the Bay of Biscay continental shelf Two issues are investigated, depending on the variable used, to constrain the winter to spring phytoplankton production for the year 2001. First, SPM data is used as forcing data to correct the corresponding state variable of our model. This allows the realistic simulation of the light limited bloom at the end of February 2001, as observed with...
Tipo: Text Palavras-chave: Bay of Biscay; SeaWiFS; Parameter estimation; Chlorophyll; Suspended Particulate Matter; Physical biogeochemical modelling.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2493.pdf
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Growth and phosphorus uptake by the toxic dinoflagellate Alexandrium catenella (dinophyceae) in response to phosphate limitation ArchiMer
Jauzein, Cecile; Labry, Claire; Youenou, Agnes; Quere, Julien; Delmas, Daniel; Collos, Yves.
Alexandrium catenella (Whedon et Kof.) Balech has exhibited seasonal recurrent blooms in the Thau lagoon (South of France) since first reported in 1995. Its appearance followed a strong decrease (90%) in phosphate (PO(4)3-) concentrations in this environment over the 1970-1995 period. To determine if this dinoflagellate species has a competitive advantage in PO(4)3--limited conditions in terms of nutrient acquisition, semicontinuous cultures were carried out to characterize phosphorus (P) uptake by A. catenella cells along a P-limitation gradient using different dilution rates (DRs). Use of both inorganic and organic P was investigated from measurements of 33PO(4)3- uptake and alkaline phosphatase activity (APA), respectively. P status was estimated from...
Tipo: Text Palavras-chave: Alexandrium catenella; Alkaline phosphatase; Limitation; Phosphorus; Semicontinuous cultures.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12641/9570.pdf
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Microbial interactions in marine water amended by eroded benthic biofilm: A case study from an intertidal mudflat ArchiMer
Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J..
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (< 0.6 μ- and < 10 μ filtrates) were diluted either with < 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with < 30 kDa-benthic compounds (BC). The kinetics of heterotrophic...
Tipo: Text Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf
Registros recuperados: 35
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