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| Registros recuperados: 49 | |
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| Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie. |
| An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages... |
| Tipo: Text |
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf |
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| Alzieu, Claude; Abarnou, Alain; Bassoullet, Philippe; Boutier, Bernard; Chiffoleau, Jean-francois; Crenn, Isabelle; Derrien, Annick; Erard-le Denn, Evelyne; Gourmelon, Michele; Guiliaud, Jean-françois; Silva Jacinto, Ricardo; Le Cann, Pierre; Le Guyader, Françoise; Le Hir, Pierre; L'Yavanc, Jacky; Mauvais, Jean-louis; Menard, Dominique; Michel, Pierre; Monbet, Yves; Pommepuy, Monique; Quiniou, Francoise. |
| Port activities require continuous or occasional dredging of considerable quantities of sediments : approximately 50 million m3 along the French coastline. A large proportion of the dredgings is authorized for dumping at sea in geographically delimited areas and following an impact evaluation of their potential harmfulness to the marine environment. The environmental risks assessment linked to dredging operations relies on knowledge related to several scientific disciplines contributing to changes in the regulations. This work, intended both for managers and for environmental scientists and engineers, is designed to take stock of the current state-of-the-art in the field of sediment contamination and related ecotoxicological hazards, along with a review of... |
| Tipo: Text |
Palavras-chave: Regulations; Management; Impact; Contaminants; Dredging; Réglementation; Gestion; Impact; Contaminants; Dragages. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-1040.pdf |
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| Gourmelon, Michele; Caprais, Marie-paule; Segura, Raphael; Le Mennec, Cecile; Lozach, Solen; Piriou, Jean-yves; Rince, A. |
| In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the Bacteroidales markers was evaluated on human and animal (bovine, pig, sheep, and bird) feces. Two human-specific markers (HF183 and HF134), one ruminant-specific marker (CF193'), and one pig-specific marker (PF163) showed a high level of specificity (>90%). However, the data suggest that the proposed ruminant-specific CF128 marker would be better described as an animal marker, as it was observed in all bovine and sheep feces and 96% of pig feces. F RNA... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3535.pdf |
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| Mieszkin, Sophie; Furet, Jean-pierre; Corthier, Gerard; Pommepuy, Monique; Le Saux, Jean-claude; Bougeard, Morgane; Hervio Heath, Dominique; Gourmelon, Michele. |
| The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent microbial source tracking method was selected: Bacteroidales host-specific 16S rRNA gene markers by real-time Polymerase Chain Reaction (PCR). A human-specific Bacteroidales marker (Hum-1-Bac) was designed. Tested on faecal samples, the Hum-1-Bac marker showed 95 % sensitivity and 95 % specificity (n= 80). Average values (± STD) of the Hum-1-Bac marker were found to be 7.3 ± 1.4 16S rRNA gene copies per g wet faeces in human faeces samples (n=10) and 5.7 ± 1.3 log10... |
| Tipo: Text |
Palavras-chave: Microbial Source Tracking; Host-Specific Bacteroidales Marker; 16S rRNA Gene; Real-Time PCR; Faecal Contamination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00021/13259/10306.pdf |
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| Petit, Fabienne; Clermont, Olivier; Delannoy, Sabine; Servais, Pierre; Gourmelon, Michele; Fach, Patrick; Oberle, Kenny; Fournier, Matthieu; Denamur, Erick; Berthe, Thierry. |
| The aim of this study was to investigate the diversity of the E. coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E. coli multi-resistant to antibiotics, evidenced a fecal contamination originating from animals at upstream rural sites, and from humans at downstream urban sites. A strong spatial... |
| Tipo: Text |
Palavras-chave: Water; Sediment; Pathogenic E. coli; Virulence gene; Shiga toxin. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00378/48887/49328.pdf |
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| Guillier, Laurent; Gourmelon, Michele; Lozach, Solen; Cadel-six, Sabrina; Vignaud, Marie-léone; Munck, Nanna; Hald, Tine; Palma, Federica. |
| The partitioning of pathogenic strains isolated in environmental or human cases to their sources is challenging. The pathogens usually colonize multiple animal hosts, including livestock, which contaminate the food-production chain and the environment (e.g. soil and water), posing an additional public-health burden and major challenges in the identification of the source. Genomic data opens up new opportunities for the development of statistical models aiming to indicate the likely source of pathogen contamination. Here, we propose a computationally fast and efficient multinomial logistic regression source-attribution classifier to predict the animal source of bacterial isolates based on ‘source-enriched’ loci extracted from the accessory-genome profiles... |
| Tipo: Text |
Palavras-chave: Environmental contamination; Multinomial logistic regression; Pangenome-wide enrichment analysis; Source attribution; Salmonella Typhimurium.. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73632/73072.pdf |
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| Mauffret, Aourell; Mieszkin, Sophie; Morizur, Mael; Alfiansah, Yustian Rovi; Lozach, Solen; Gourmelon, Michele. |
| We assessed the capacity of real-time PCR markers to identify the origin of contamination in shellfish. Oyster, cockles or clams were either contaminated with fecal materials and host-associated markers designed from Bacteroidales or Catellicoccus marimammalium 16S RNA genes were extracted from their intravalvular liquid, digestive tissues or shellfish flesh. Extraction of bacterial DNA from the oyster intravalvular liquid with FastDNA spin kit for soil enabled the selected markers to be quantified in 100% of artificially contaminated samples, and the source of contamination to be identified in 13 out of 38 naturally contaminated batches from European Class B and Class C areas. However, this protocol did not enable the origin of the contamination to be... |
| Tipo: Text |
Palavras-chave: Microbial source tracking; Shellfish; Bacteroidales; Real-time PCR; Intravalvular liquid; Digestive tissues. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00137/24776/25188.pdf |
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| Gourmelon, Michele; Derrien, A.; Crenn, Isabelle; Loaec, Solen. |
| Des bactéries fécales telles que les Escherichia coli ou les coliformes thermotolérants ont été recherchées dans des sédiments côtiers vaseux (prélevés sur deux sites différents: Morlaix et St Pol de Léon; Finistère). Ces vases, sous influence de rejets polluants, de par leur richesse en matière organique et en particules de faible taille sont susceptibles d'héberger ces bactéries. Une partie de l'étude a consisté à comparer différentes techniques de relargage des bactéries des particules de sédiment décrites dans la littérature. |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00081/19213/16808.pdf |
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| Breton, Marguerite; Caprais, Marie-paule; Derrien, A.; Douillet, P; Dubois, E; Dupray, E; Gourmelon, Michele; Guillerm, Denise; Kempf, Marc; Lazure, P.; Le Guyader, Soizick; Le Menn, Remi; Menard, Dominique; Pommepuy, Monique; Salomon, Jean-claude. |
| Les départements et territoires d'outre mer sont généralement l'objet d'enjeux économiques importants, et de conflits d'activités liés au développement touristique, aquacole, agricole et industriel. Les études font généralement état d'une lente dégradation de l'environnement marin avec diminution des coraux et de la faune associée, au profit d'une augmentation de la couverture algale. On constate parfois un envasement ou un ensablement des sites, la présence dans l'eau de polluants organiques, dont des pesticides, des métaux ainsi que des microorganismes d'origine fécale. En Martinique, ce dernier aspect, lié à l'assainissement des zones rurales, urbaines et touristiques est actuellement considéré comme une préoccupation majeure pour les prochaines années.... |
| Tipo: Text |
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| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00132/24303/22301.pdf |
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| Pourcher, Anne-marie; Jarde, Emilie; Jeanneau, Laurent; Solecki, Olivia; Jadas-hecart, Alain; Caprais, Marie-paule; Durand, Gaëlle; Gourmelon, Michele. |
| La dégradation de la qualité des eaux par la pollution microbiologique représente un problème majeur de santé publique, notamment dans les eaux ou s'exercent des activité humaines. Afin de définir les actions prioritaires à mener, une meilleure connaissance de l'origine des pollution fécales est nécessaire. Cet article nous présente le développement d'une méthode innovante utilisant des marqueurs chimiques et microbiologiques permettant de différencier trois sources majeures de contamination des eaux de baignade en Bretagne: les déjections humaines, bovines et porcines. |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00315/42604/41980.pdf |
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| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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| Mauffret, Aourell; Caprais, Marie-paule; Gourmelon, Michele. |
| The relevance of three host-associated Bacteroidales markers (HF183, Rum2Bac, and Pig2Bac) and four F-specific RNA bacteriophage genogroups (FRNAPH I to IV) as microbial source tracking markers was assessed at the level of a catchment (Daoulas, France). They were monitored together with fecal indicators (Escherichia coli and enterococci) and chemophysical parameters (rainfall, temperature, salinity, pH, and turbidity) by monthly sampling over 2 years (n = 240 water samples) and one specific sampling following an accidental pig manure spillage (n = 5 samples). During the 2-year regular monitoring, levels of E. coil, enterococci, total F-specific RNA bacteriophages, and the general Bacteroidales marker AllBac were strongly correlated with one another and... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00089/20009/17680.pdf |
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| Baliere, Charlotte; Rince, A.; Delannoy, S.; Fach, P.; Gourmelon, Michele. |
| Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00344/45514/45076.pdf |
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| Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele. |
| During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods.... |
| Tipo: Text |
Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf |
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| Gourmelon, Michele. |
| L’originalité de cette étude était de rechercher si ces bactéries pouvaient être présentes dans l’environnement marin (tout particulièrement dans les coquillages) en développant ou adaptant des méthodes de détection. La présence de ces bactéries pourrait peut-être expliquer certaines TIACs liées à la consommation de coquillages dont l’agent responsable n’a pas été identifié. |
| Tipo: Text |
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| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00066/17752/15274.pdf |
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| Mieszkin, Sophie; Yala, Jean Fabrice; Joubrel, Rozenn; Gourmelon, Michele. |
| Aims: The aims of this study were to evaluate the host-specific distribution of Bacteroidales 16S rRNA gene sequences from human- and animal-related effluents and faeces, and to define a ruminant-specific marker. Methods and Results: Bacteroidales 16S rRNA gene clone libraries were constructed from samples of effluent (sewage, bovine manure and pig slurry) and faeces (human, bovine, pig and wild bird), using PCR primers targeting order Bacteroidales. The phylogenetic analysis revealed six main distinct human-, bovine-, pig- and wild bird-specific clusters. From the bovine-specific cluster II, we designed a ruminant-specific marker, Rum-2-Bac, and this showed 97% sensitivity (n = 30) and 100% specificity (n = 40) when tested by TaqMan (R) real-time PCR.... |
| Tipo: Text |
Palavras-chave: 16S rRNA gene; Bacteroidales; Faecal contamination; Microbial Source Tracking; Sewage and manure. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00000/11156/8235.pdf |
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| Gourmelon, Michele; Lozach, Solen; Garry, Pascal; Balliere, Clémence; Sauvageot, Nicolas; Le Hello, Simon; Rince, Alain. |
| Shellfish farming is an important economic activity in the Brittany and Normandy regions. However, a part of the production sites corresponds to relatively sensitive areas where the presence of faecal microorganisms is a major concern for shellfish and constitutes a possible health risk. Indeed, shellfish bioaccumulates in their tissues pathogenic contaminants present in water and can cause food-borne diseases such as salmonellosis. During a two-year study, we evaluated the presence of faecal indicators, measured the prevalence of Salmonella spp., isolated and characterized Salmonella spp. from three French shellfish-harvesting areas (shellfish and sediment) and their watersheds (from river water samples). |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00341/45199/44609.pdf |
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| Registros recuperados: 49 | |
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