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| Registros recuperados: 40 | |
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| Ky, Chin-long; Le Pabic, Lore; Koua, Manaarii Sham; Molinari, Nicolas; Nakasai, Seiji; Devaux, Dominique. |
| The black-lipped pearl oyster, Pinctada margaritifera is the most important farmed mollusc species in French Polynesia. Donor oyster selection among wild P. margaritifera individuals, chosen according to their inner shell colour, makes it possible to obtain the broadest range of cultured pearl colours of any species. This study demonstrates the relative influence of using black [B] or red [R] outer shell phenotypes, combined with green [G] or yellow [Y] inner shell phenotypes, on pearl darkness level, colour categories and lustre. A large scale grafting experiment was designed and carried out over five grow-out locations, covering three archipelagos: Tuamotus, Society and Gambier. Results revealed that the [B + G] phenotypes may be used as donors to... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Shell colour phenotype; Pearl colour; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00285/39637/38129.pdf |
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| Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe. |
| Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of... |
| Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf |
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| Le Luyer, Jeremy; Auffret, Pauline; Quillien, Virgile; Leclerc, Nicolas; Reisser, Celine; Vidal-dupiol, Jeremie; Ky, Chin-long. |
| Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the Pinctada genus, through the biomineralization properties of pearl sac tissue. Improvement of P. margaritifera pearl quality is one of the biggest challenges that Polynesian research has faced to date. To achieve this goal, a better understanding of the complex mechanisms related to nacre and pearl formation is essential and can now be approached through the use of massive parallel sequencing technologies. The aim of this study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that had producing pearls with high and low quality. For this purpose, a comprehensive reference transcriptome of P. margaritifera was built based on... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Biomineralization; Pearl quality; Pearl sac; RNA-seq. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00475/58695/61207.pdf |
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| Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Duboisset, Julien; Dicko, Hamadou; Durand, Patrick; Quintric, Laure; Planes, Serge; Vidal-dupiol, Jeremie. |
| The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Color; Pigment; Transcriptomics; Raman spectroscopy. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00685/79671/82446.pdf |
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| Ky, Chin-long; Le Moullac, Gilles. |
| Size is the most important and valuable quality trait of cultured pearls produced by the black-lipped pearl oyster, Pinctada margaritifera. In French Polynesia, several breeding programmes have been started that aim to improve this size trait, which is highly related to shell growth rate in both recipient and donor oysters. Shell growth rate dictates the time of grafting, size of implanted nuclei and bio-mineralisation potential of the mantle and pearl sac. We assessed shell growth rate through routine digital shell biometric analysis on 22 hatchery families produced between 2005 and 2008. These included full-sib families and half-sib families derived from polyandry (one dam crossed with two or more sires). Results showed that: 1) a significant family... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Hatchery-produced families; Growth performance; Shell diameter; Cultured pearl size. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49810/50384.pdf |
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| Blay, Carole; Planes, Serge; Ky, Chin-long. |
| Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters. Several phenotypic measurements were made: 1) donor family growth performance from shell size records, 2) pearl size and corresponding quality traits, and 3) expression of some genes related to... |
| Tipo: Text |
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| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00444/55593/57220.pdf |
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| Dubé, Caroline; Ky, Chin-long; Planes, Serge. |
| Elucidating the role of prokaryotic symbionts in mediating host physiology has emerged as an important area of research. Since oysters are the world’s most heavily cultivated bivalve molluscs, numerous studies have applied molecular techniques to understand the taxonomic and functional diversity of their associated bacteria. Here, we expand on this research by assessing the composition and putative functional profiles of prokaryotic communities from different organs/compartments of the black-lipped pearl oyster Pinctada margaritifera, a commercially important shellfish valued for cultured pearl production in the Pacific region. Seven tissues, in addition to mucous secretions, were targeted from P. margaritifera individuals: the gill, gonad, byssus gland,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Microbiome; Tissue-specific bacterial communities; 16S rRNA gene sequencing; Functional profiling prediction. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61670/65589.pdf |
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| Molinari, Nicolas; Abou-badra, Mathieu; Marin, Gregory; Ky, Chin-long; Amador, Noemi; Gamez, Anne Sophie; Vachier, Isabelle; Bourdin, Arnaud. |
| Background: COPD is largely under-diagnosed and once diagnosed usually at a late stage. Early diagnosis is thoroughly recommended but most attempts failed as the disease is marginally known and screening marginally accepted. It is a rare cause of concern in primary care and spirometry is not very common. Exhaled carbon monoxide (eCO) is a 5-seconds easy-to-use device dedicated to monitor cigarette smoke consumption. We aimed to assess whether systematic eCO measurement in primary care is a useful tool to improve acceptance for early COPD diagnosis. Methods: This was a two-center randomized controlled trial enrolling 410 patients between March and May, 2013. Whatever was the reason of attendance to the clinic, all adults were proposed to measure eCO during... |
| Tipo: Text |
Palavras-chave: COPD; Screening; Exhaled CO; Smoking; Behaviour. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38142/36280.pdf |
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| Ky, Chin-long; Nakasai, Seiji; Parrad, Sophie; Broustal, Floriane; Devaux, Dominique; Louis, Patricia. |
| Cultured pearl production from Pinctada margaritifera uses the biomineralization capacities of the mantle graft, the saibo, which is usually obtained from only the middle mantle section of the donor oyster. To evaluate the potential for using other parts of the mantle, this study explores and describes the cultured pearl quality traits, pearl size, shape, surface defects and colour parameters obtained with saibo from the entire length of the mantle, comprising the four following sections: 1) posterior, 2) connection with the gills, 3) middle, (the section usually used commercially), and 4) anterior. Rates of nucleus retention and oyster mortality were also recorded and compared between sections. For this, two experimental grafts were designed and conducted... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Pearl quality traits; Saibo position; Mantle tissue; Phenotype. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00439/55030/56461.pdf |
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| Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Lo, Cédrik; Cabral, Philippe. |
| The top aquaculture species in French Polynesia is Pinctada margaritifera, a mollusc grown for the production of a unique gem: the black pearl. One of the challenges facing the pearl farming industry is to "produce less but better pearls" through genetic improvement. An experimental hatchery system was used to generate full-sib families to be tested for their potential as donor "oysters". A large-scale grafting experiments was done and seven cultured pearl quality traits: grade, surface defects, lustre, darkness level, visual colour categories, circles and shape categories were recorded. Our results revealed, for the first time, significant phenotypic relationships between these quality traits. The grade A cultured pearl class had the largest proportion of... |
| Tipo: Text |
Palavras-chave: Pearl "oyster"; Pinctada margaritifera; Donor "oyster"; Cultured pearl quality; Pearl grade; Pearl colour; Pearl shape; Selective breeding. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30130/28592.pdf |
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| Ky, Chin-long; Okura, Retsu; Nakasai, Seiji; Devaux, Dominique. |
| In French Polynesia, the aquaculture of Pinctada margaritifera (Linnaeus, 1758) covers a large maritime exploitation area, spread over nearly 20 degrees latitude and longitude, with numerous pearl farms located in three archipelagos (Gambier, Society, and Tuamotu). As these archipelagos have specific seasonal temperature patterns each year, pearl oysters are subject to disparate and contrasting environmental regimes. This study aimed to examine the specificity of commercial pearl quality traits (n ¼ 2,236 samples) at the archipelago scale, in such a way as to provide preliminary data to design the most appropriate strategy for the distribution of hatchery-produced phenotypes. A large and standardized grafting experiment using the same donor phenotype was... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Large-scale geographic effects; Pearl color; Pearl grade; Pearl shape; Pearl size. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00357/46798/46665.pdf |
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| Latchere, Oihana; Fievet, Julie; Lo, Cedrik; Schneider, Denis; Dieu, Stephanie; Cabral, Philippe; Belliard, Corinne; Ky, Chin-long; Gueguen, Yannick; Saulnier, Denis. |
| The present study investigated the effect of electrolysis on the biomineralization capacities of juveniles of the mollusk Pinctada margaritifera for the first time. Size-selected individuals from two groups, “Medium” and “Large”, from a multi-parental family produced in a hatchery system were subjected to electrolysis under a low voltage current over a nine-week experimental period. The growth of the juveniles was individually monitored and assessed weekly by wet weight and shell height measurements. At the end of the experiment, mantle tissue was sampled for biomineralization-related gene expression analysis. Electrolysis significantly increased pearl oyster growth in terms of shell height and wet weight for Large juveniles from the 5th and the 2nd week,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Electrolysis; Biomineralization; Gene expression. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00343/45410/44924.pdf |
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| Blay, Carole; Planes, Serge; Ky, Chin-long. |
| Grafting associates two distinct genotypes, each of which maintains its own genetic identity throughout the life of the grafted organism. Grafting technology is well documented in the plant kingdom, but much less so in animals. The pearl oyster, Pinctada margaritifera, produces valuable pearls as a result of the biomineralisation process of a mantle graft from a donor inserted together with a nucleus into the gonad of a recipient oyster. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed using donor and recipient oysters monitored for their growth traits. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00387/49809/50382.pdf |
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| Ky, Chin-long; Demmer, Jonathan; Blay, Carole; Lo, Cedrik. |
| Pinctada margaritifera is an economically important marine bivalve species for cultured pearl production in French Polynesian aquaculture. In order to evaluate the influence of donor oyster age on pearl quality traits, experiments were conducted over 6 years using both grafts and surgreffe operations. At harvest, 6 pearl quality traits were recorded and compared: surface defects, luster, grade, darkness level, and visual color. Analyzing the quality traits of pearls harvested in the initial graft process and those of pearls obtained from surgreffe experiments allowed a comparison of the influence of pearl sac cells originating from the initial mantle graft, which aged together with their recipient oysters. The results demonstrated a significant decrease... |
| Tipo: Text |
Palavras-chave: Pearl oyster age; Pinctada margaritifera; Surgreffe; Pearl grade; Pearl colour. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00283/39472/37953.pdf |
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| Ky, Chin-long; Broustal, Floriane; Koua, Manaarii Sham; Quillien, Virgile; Beliaeff, Benoit. |
| Understanding the respective roles played by donor and recipient pearl oysters in pearl quality determination in relation to the environment is a challenge for the pearl industry. In most Pinctada species, pearl size is mainly related to recipient oyster growth performance, but also relies to some extent on the biomineralisation activity of the pearl sac, a tissue that originates from the donor oyster mantle. We examined donor effect on pearl size in response to culture in the lagoons on Arutua and Apataki atolls. Overall nacre weight and thickness were greater in Arutua than in Apataki, but sensitivity to the environment differed between donors. Some donors were associated with significantly heavier and thicker nacre in Arutua (I group), while others had... |
| Tipo: Text |
Palavras-chave: Biomineralisation; Environment; Pearl oyster; Pearl size; Pinctada margaritifera. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00425/53699/54610.pdf |
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| Ky, Chin-long; Blay, Carole; Aiho, Vaite; Cabral, Philippe; Le Moullac, Gilles; Lo, Cedrik. |
| In French Polynesia, the aquaculture of P. margaritifera is carried out in numerous grow-out sites, located over three archipelagos (Gambier, Society and Tuamotu). To evaluate the impact of macro-geographical effects of these growing sites on pearl quality traits, five hatcheries produced families were used as homogeneous donor oysters in an experimental graft. The molluscs were then reared in two commercial locations: Tahaa island (Society) and Rangiroa atoll (Tuamotu). At harvest, eight pearl quality traits were recorded and compared: surface defects, lustre, grade, circles, shape categories, darkness level, body and secondary colour and visual colour categories. Overall inter-site comparison revealed that: 1) all traits were affected by grow-out... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl quality; Grow-out location; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00276/38687/37186.pdf |
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| Ky, Chin-long; Lo, Cedrik; Planes, Serge. |
| The pearl oyster Pinctada margaritifera has the specific ability to produce pearls with the widest range of colours among all pearl oyster species. This pearl colour diversity originates from the mantle biomineralising tissue (graft) of the donor oyster, which is originally responsible for the variety of colours of the inner shell surface. This study aimed to: 1) assess the geographic distribution and establish a first stocklist of the colourful oyster phenotypes used as donors in French Polynesia, and 2) investigate the phenotypic relation between inner shell colouration and the corresponding colour of harvested pearls. With the support of a pearl farmers' network, we investigated the different donor phenotype frequencies among five collection sites (Ahe,... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00354/46474/46249.pdf |
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| Le Pabic, Lore; Parrad, Sophie; Sham Koua, Manaarii; Nakasai, Seiji; Saulnier, Denis; Devaux, Dominique; Ky, Chin-long. |
| Size is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera. As this pearl aquaculture is carried out at numerous grow-out sites, this study analyzes the environmental influence on pearl size parameters (nacre weight and thickness) in relation to the recipient oyster biometric parameters (shell thickness, height, width, and oyster weight) at harvest time. Toward this end, an experimental graft was designed by using a homogeneous donor oyster phenotype. The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations. Overall inter-site comparisons revealed that the cultured pearl size (N=2168) and the biometric parameters of the recipient oysters were... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl size; Donor oysters; Recipient oysters; Gene expression; Environmental influences. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00321/43191/42738.pdf |
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| Registros recuperados: 40 | |
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