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| Registros recuperados: 57 | |
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| Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas. |
| Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the... |
| Tipo: Text |
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf |
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| Leprêtre, Maxime; Faury, Nicole; Segarra, Amelie; Claverol, Stéphane; Degremont, Lionel; Palos-ladeiro, Mélissa; Armengaud, Jean; Renault, Tristan; Morga, Benjamin. |
| Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and... |
| Tipo: Text |
Palavras-chave: Proteomics; Interactions; Antiviral response; OsHV-1; Pacific oysters. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00669/78075/80307.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan. |
| Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen |
| Tipo: Text |
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf |
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| Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio. |
| Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland.... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf |
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| Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
| The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
| Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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| Dégremont, Lionel; Morga, Benjamin; Trancart, Suzanne; Pépin, Jean-francois. |
| The ostreid herpesvirus(OsHV-1) is one of the major diseases that affect the Pacific oyster Crassostrea gigas. Selective breeding programs were recently shown to improve resistance easily to OsHV-1 infections in spat, juvenile, and adult oysters. Nevertheless, this resistance has never been investigated in larvae, whereas this developmental stage has crucial importance for the production of commercial hatcheries, as well as explaining the abundance of spatfall. A first trial tested several viral suspensions at several concentrations using contaminated water with OsHV-1 in 4- and 10-day-old larvae that we reproduced from an unselected broodstock. In follow up on the results, one viral suspension at a final concentration of 10+6 OsHV-1 DNA copies per L was... |
| Tipo: Text |
Palavras-chave: Larvae; Crassostrea gigas; OsHV-1; Disease resistance; Mortality. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00313/42441/41796.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
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| Martenot, Claire; Faury, Nicole; Morga, Benjamin; Degremont, Lionel; Lamy, Jean-baptiste; Houssin, Maryline; Renault, Tristan. |
| Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection.... |
| Tipo: Text |
Palavras-chave: OsHV-1; Antiviral antibodies; Dextran sulfate; Interaction; Crassostrea gigas; Infection. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00501/61273/64847.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle. |
| Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf |
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| Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Dégremont, Lionel; Burge, Colleen A.; Mancilla Cortez, Daniel; Friedman, Carolyn S.; Fleener, Gary B.; Dumbauld, Brett R.; Langdon, Chris. |
| Background Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in... |
| Tipo: Text |
Palavras-chave: Ostreid herpesvirus 1; Crassostrea gigas; Heritability; Breeding. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00598/70974/69206.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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| Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A.. |
| Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the... |
| Tipo: Text |
Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf |
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| Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Costes, Louis; Degremont, Lionel; Garcia, Celine; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Morga, Benjamin; Normand, Julien; Robert, Stephane; Saunier, Alice; Soletchnik, Patrick; Travers, Marie-agnes. |
| Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015 et 2016 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2017, les trois objectifs principaux étaient de : i) poursuivre la description du phénomène de mortalité ainsi que le contexte environnemental associé en effectuant une nouvelle campagne d’études et d’observations in situ, ii) tester des hypothèses... |
| Tipo: Text |
Palavras-chave: Moules; Mytilus edulis; Mortalité; Statut cytogénétique; Anomalies génomiques; Populations; Facteurs environnementaux; Ecologie microbienne. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00503/61503/65337.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
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| Bai, Chang-ming; Morga, Benjamin; Rosani, Umberto; Shi, Jie; Li, Chen; Xin, Lu-sheng; Wang, Chong-ming. |
| Ostreid herpesvirus 1 (OsHV-1) is an important pathogen associated with mass mortalities of cultivated marine mollusks worldwide. Since no cell line allows OsHV-1 replication in vitro, it is difficult to isolate enough high-purity viral DNA for High-Throughput Sequencing (HTS). We developed an efficient approach for the enrichment of OsHV-1 DNA for HTS with long-range PCR. Twenty-three primer pairs were designed to cover 99.3% of the reference genome, and their performances were examined on ten OsHV-1 infected samples. Amplicon mixtures from six successfully amplified samples were sequenced with Illumina platform, and one of them (ZK0118) was also sequenced with the PacBio platform. PacBio reads were assembled into 2 scaffolds compared to 9−68 scaffolds... |
| Tipo: Text |
Palavras-chave: Long-range PCR; OsHV-1; High-throughput sequencing; Evolution; Diversity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00464/57566/64184.pdf |
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| Degremont, Lionel; Maurouard, Elise; Godfrin, Yoann; Travers, Marie-agnes; Morga, Benjamin; Lamy, Jean-baptiste; Benabdelmouna, Abdellah. |
| Depuis 2012, le nombre de cas de mortalités d’huîtres creuses adultes (Crassostrea gigas) rapportés dans le cadre des réseaux de surveillance de l’Ifremer, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Les objectifs de cette étude étaient (1) d’étudier la sensibilité des huîtres C. gigas à une infection à V. aestuarianus pour des lots diploïdes et triploïdes et (2) déterminer la réponse à la sélection pour une meilleure résistance à V. aestuarianus pour des huîtres C. gigas. Neuf stocks ont été utilisés, répartis en trois niveaux de sélection, chacun étant donc répliqué par trois pontes. Pour chaque stock, un lot diploïde (2n), un lot triploïde (3n_chim) produit par la rétention d’un globule polaire, et trois lots... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; OsHV-1; Sélection; Résistance; Triploïdes; Huître creuse; Crassostrea gigas; Vibrio aestuarianus; OsHV-1; Selective breeding; Disease resistance Pacific oyster; Crassostrea gigas; Triploids. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51530/52140.pdf |
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| Bai, Chang-ming; Wang, Qing-chen; Morga, Benjamin; Shi, Jie; Wang, Chong-ming. |
| We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3 days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24 months old). |
| Tipo: Text |
Palavras-chave: Scapharca broughtonii; Ostreid herpesvirus SB strain; OsHV-1-SB; Experimental infection; Viral quantity. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00360/47119/47081.pdf |
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| Registros recuperados: 57 | |
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