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Bacterial communities and syntrophic associations involved in anaerobic oxidation of methane process of the Sonora Margin cold seeps, Guaymas Basin ArchiMer
Vigneron, Adrien; Cruaud, Perrine; Pignet, Patricia; Caprais, Jean-claude; Gayet, Nicolas; Cambon-bonavita, Marie-anne; Godfroy, Anne; Toffin, Laurent.
The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previous study on the same samples identified anaerobic oxidation of methane (AOM) as the potential dominant archaeal process in these Sonora Margin sediments, probably catalysed by three clades of archaeal anaerobic methanotrophs (ANME-1, ANME-2 and ANME-3) associated with bacterial syntrophs. In this study, molecular surveys and microscopic observations investigating the diversity of Bacteria involved in AOM...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00168/27921/27279.pdf
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Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys ArchiMer
Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf
Registros recuperados: 22
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