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| Registros recuperados: 48 | |
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| Maggi, Pierre; Truquet, Philippe; Mornet, Francoise; Lassus, Patrick. |
| Une campagne de prélèvements hydrologiques a été réalisée en juillet 1991 en baie de Seine sur le N/O THALIA. Elle visait à connaître la distribution du phytoplancton en fonction des paramètres physicochimiques du milieu et plus particulièrement par rapport à l'extension du panache de la Seine. Bien que les espèces toxiques recherchées (Dinophysis spp.) aient été absentes au cours de cette opération, l'analyse et le traitement des données recueillies sur une radiale côtière située entre le Havre et Antifer ont permis de discriminer les espèces à affinité estuarienne ou marine présentes pendant cette période. Les diatomées étaient largement dominantes, en particulier Skeletonema costatum et Rhizosolenia stolterfothii, alors que les dinoflagellés étaient... |
| Tipo: Text |
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| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00064/17535/15053.pdf |
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| Marcaillou-le Baut, Claire; Masselin, Pierre; Bohec, Madeleine; Truquet, Philippe; Amzil, Zouher; Pouchus, Francois; Le Boterff, Jean; Verbist, Jean-françois; Vernoux, Jean-paul; Marais, Chantal; Simon, Jean-françois; Nizard, Gabrielle; Mercier, Géraldine; Choumiloff, Roxelan; Baron, Bruno. |
| Cette étude est le résultat d'un travail de trois équipes: elle porte sur la comparaison du test souris avec différents bioessais déjà pratiqués en routine dans les laboratoires participants. Ces bioessais sont : - un test sur micro crustacé dont la méthodologie est normalisée et qui est très utilisé en écotoxicologie aquatique ; - un test de cytotoxicité à court terme qui a été mis au point pour cette étude. |
| Tipo: Text |
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| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00139/25035/23144.pdf |
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| Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Truquet, Philippe; Haberkorn, Hansy; Lassus, Patrick; Barille, Laurent; Amzil, Zouher. |
| The aim of this study was to evaluate the importance of biotransformation of paralytic shellfish toxins during the detoxification process in contaminated oysters. Mathematical models based upon the detoxification patterns of digestive gland and other tissues were developed. It was demonstrated that biotransformations do not seem to play an important role in digestive gland or other tissue detoxification kinetics with our data set. Moreover, different toxin transfers from digestive gland toward other tissues were investigated. No significant transfer was highlighted in our data set. These first conclusions were drawn after comparing the results obtained from 13 biotransformations and identifiable transfer scenarios. Finally, to determine a more robust... |
| Tipo: Text |
Palavras-chave: Detoxification kinetics modelling; Biotransformation of saxitoxin analogues; Oyster. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00049/16047/14309.pdf |
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| Baron, Regis; Couedel, Marielle; Joret, Camille; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Lassus, Patrick. |
| A simple system was used to simulate the effect of alternating toxic (paralytic shellfish poisoning toxins) and non-toxic microalgal diets on oyster feeding behaviors and rates of toxin accumulation. These experimental conditions were meant to reflect, to some extent, the incoming and outgoing fluxes of toxic algae observed at the mouth of the Penze estuary (Northern Brittany, France). Physiological and toxicological parameters were estimated based oil fluorescence measurements recorded continuously at the outlet of each experimental tank, which contained a single oyster. Q(tox), this variable describes toxin uptake in oysters, it was used (instead of the toxin ingestion rate): i) in simple graphical analyses, ii) as well as in one- and two-compartment... |
| Tipo: Text |
Palavras-chave: Skeletonema costatum; Alexandrium minutum; Fluorescence; Modeling; Kinetics; Toxin uptake; Shellfish toxin; PSP. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1400.pdf |
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| Lassus, Patrick; Chagot, Dominique; Miossec, Laurence; Bocquene, Gilles; Truquet, Philippe. |
| Tetrachloroethylene, a chlorinated solvent, is detected in the plume of East-Toulon domestic wastes treatment plant. Its subletal toxicity was tested du ring 31 days in a flow-through system at Pen Bron aquaculture centre, using swampshrimp Palaemonetes varians as test organism. Under expérimental conditions : natural variations in temperature and salinity , normal pH, ammonia and dissolved oxyg~n values, running tetrachloroethylene concentrations (10, 100 and 500 µg.l-1) induced neither mortalities nor any change in molting frequencies of experirnented animaIs. Histology of gills in contaminated animaIs showed several non specific deteriorations which could be observed either in control or assay animaIs. Necrosis levels nevertheless, seem to increase in... |
| Tipo: Text |
Palavras-chave: Palaemonetes varians; Crustacés; Tetrachloroethylene; Flux continu; Rejets urbains; Histologie; Nécroses; Palaemonetes varians; Crustacees; Tetrachloroethylene; Flow through system; Urban wastes; Histology; Necrosis. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00156/26707/24782.pdf |
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| Jauffrais, Thierry; Contreras, Andrea; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Tillmann, Urban; Hess, Philipp. |
| Azadinium spinosum, a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic (A. spinosum) or non-toxic (Isochrisis aff. galbana (T-Iso)) diets. Furthermore, AZA... |
| Tipo: Text |
Palavras-chave: Bivalve molluscs; Mussel; Ecophysiology; AZA biotransformation; AZA accumulation; Trophic transfer; Dinoflagellate; Azaspiracid. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20174/17832.pdf |
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| Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Haberkorn, Hansy; Soudant, Philippe; Truquet, Philippe; Lassus, Patrick. |
| The purpose of this study was to assess paralytic phycotoxin uptake in diploid and triploid oysters at two stages of their sexual cycle corresponding to their status in early summer (June) and winter (November). Samples of diploid and triploid oysters were exposed to a toxic culture of Alexandrium minutum for 4 days in each season. No significant differences in filtration or clearance rates were observed during either November or June experiments. When diploid oysters were at resting stage (November), toxin uptake showed no significant difference between the ploidy classes. In contrast, when the diploid oysters were at the peak of their sexual maturation (June), the triploid oysters were seen to accumulate almost double the amount of paralytic toxins as... |
| Tipo: Text |
Palavras-chave: Paralytic shellfish poisoning; Crassostrea gigas; Diploid; Triploid; Contamination. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00087/19796/17487.pdf |
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| Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Naviner, Magali; Truquet, Philippe. |
| A sea water recirculating flume was especially designed in the framework of this study. It improves physiological and ecophysiological evaluation of a 25 oysters (c. gigas) set fed on PSP toxic A. minutum cultures and then detoxified with commonly used aquaculture species (I. galbana, T. suecica, S. costatum, T. weissflogii). Chlorophyll a levels are kept steady in each case and adjusted in order to reach the same food value in each tank (0,5 mg/l TPM). When exposed to the mildly toxic species A. minutum (0,5 pg.eq.STX.cel-1) oysters depict a reduced clearance rate, food uptake and shell-valves activity (compared to non-toxic algal diets). The same physiological parameters do not show any significant differences when oysters are exposed to the control al... |
| Tipo: Text |
Palavras-chave: Épuration; Crassostrea gigas; Alexandrium minutum; PSP; Écophysiologie; Depuration; C. gigas; A. minutum; PSP; Ecophysiology. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00139/25021/23126.pdf |
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| Lassus, Patrick; Le Baut, Claire; Truquet, Philippe; Bardouil, Michèle; Le Dean, Loïc; Bocquene, Gilles. |
| The growing development of thermonuclear electric plants on coastal areas drove the I.S.T.P.M. to carry out a number of impact studies. Previous works on the impact of the transit in the condensers on the phytoplankton have been published by the Pollution Biological Effects laboratory (Maggi el al., 1979 and 1980; Lassus and Maggi, 1980). Our results on the chlorophycea Dunaliella terliolecla demonstrated that thermic elevations from low temperatures stimulated its cellular development. Therefore, it seemed interesting to measure the effects of these thermic shocks on dinophyceae (or dinoflagellates), components, with diatoms, of the coastal phytoplankton, and main agents of the summer phenomena known as "red waters". |
| Tipo: Text |
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| Ano: 1982 |
URL: http://archimer.ifremer.fr/doc/1982/publication-6674.pdf |
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| Lassus, Patrick; Truquet, Philippe; Le Dean, Loic; Bardouil, Michele; Truquet, Isabelle. |
| As a part of hydrological sampLing operation in South Brittany, 8 transects for 26 sampling stations were made in order to determine horizontal, and vertical distribution of Dinophysis sacculus in the 10 m layer. Un like to 1986, results do not show any correlations between Dinophysis concentrations and stability index. That may, nevertheless, be due to lower cells densities in 1987 when compared to 1986. A thermal, gradient, lower than in 1986, is still observable in the Bouthern of Loire Mouth. And, as a last point, when chlorophyll a concentrations are higher in - 10 m layer than in surface layer (Audierne and Noirmoutier areas), it can be observed hight densities of an other toxie Dinoflagellate : Gyrodinium aureolum . |
| Tipo: Text |
Palavras-chave: Bretagne sud; Hydrologie; D. sacculus; G. aureolum; South Brittany; Hydrology; D. sacculus; G. aureolum. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00138/24921/23015.pdf |
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| Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp. |
| Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum. Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent... |
| Tipo: Text |
Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf |
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| Medhioub, Walid; Sechet, Veronique; Truquet, Philippe; Bardouil, Michele; Amzil, Zouher; Lassus, Patrick; Soudant, Philippe. |
| Growth and spirolide production of the toxic dinoflagellate Alexandrium ostenfeldii (Danish strain CCMP1773) were studied in batch culture and a photobioreactor (continuous cultures). First, batch cultures were grown in 450 mL flasks without aeration and under varying conditions of temperature (16 and 22 degrees C) and culture medium (L1, f/2 and L1 with addition of soil extract). Second, cultures were grown at 16 degrees C in 8 L aerated flat-bottomed vessels using L1 with soil extract as culture medium. Finally, continuous cultures in a photobioreactor were conducted at 18 degrees C in L1 with soil extract; pH was maintained at 8.5 and continuous stirring was applied. This study showed that A. ostenfeldii growth was significantly affected by temperature.... |
| Tipo: Text |
Palavras-chave: Alexandrium ostenfeldii; Batch; Growth; Photobioreactor; Spirolide production. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00049/16050/15784.pdf |
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| Medhioub, Walid; Gueguen, Marielle; Lassus, Patrick; Bardouil, Michele; Truquet, Philippe; Sibat, Manoella; Nejib, Medhioub; Philippe, Soudant; Mejdeddine, Kraiem; Amzil, Zouher. |
| In the Gulf of Gabes (Tunisia, Eastern Mediterranean sea), the grooved carpet shell Ruditapes decussatus has been seen to contain persistent levels of gymnodimine (GYM) for several years. The present experimental work represents the first attempt to assess detoxification kinetics of fast-acting toxins (FAT) in marine molluscs fed specific diets of non-toxic algae (Isochrysis galbana). To find an optimal detoxification method, two experiments were performed in which clams were first fed the toxic dinoflagellate Karenia selliformis to artificially contaminate them with GYM, thus simulating the effect of natural toxic episodes. As a second step, the same clams were fed a non-toxic algae, I. galbana. to speed up the detoxification process. Changes in toxin... |
| Tipo: Text |
Palavras-chave: Ruditapes decussates; Grooved carpet shell; Karenia selliformis; Gymnodimine; Toxicity; Detoxification. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2010/publication-7488.pdf |
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| Registros recuperados: 48 | |
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