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Effect of 2 % Chlorhexidine on Dentin Shear Bond Strength of Different Adhesive Systems: A 6 Months Evaluation International Journal of Morphology
Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
This study evaluated the effect of chlorhexidine dentin treatment on shear bond strength (SBS) of adhesive systems after different storages. The work included 144 third molars that had their dentin exposed and were divided in 6 groups: G1 (ASB+CHX: Adper Scotchbond 1XT + chlorhexidine 2 % prior Primer); G2(ASB); G3 (APP+CHX: Adper Prompt L-Pop + CHX); G4(APP); G5 (SBU+CHX: Single Bond Universal + CHX); and G6(SBU). Resin build-up was performed and teeth were subdivided regarding storage times (n=8): 72 h, 3 and 6 months. Next, SBS test was performed. At 72 hours, all equivalent groups (same adhesive system, different dentin treatment) showed no significant difference in SBS (P.05). Self-etch adhesive groups (with or without CHX) presented lower SBS...
Tipo: Journal article Palavras-chave: Shear bond strength; Dentin bond strength; Chlorhexidine; Adhesive systems; Adhesion.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300052
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Gross and Histologic Evaluation of Abdominal Adhesions Associated with Chromic Catgut and Polypropylene Sutured Enteropexies in Dog International Journal of Morphology
Akinrinmade,J. F; Lawal,A. O.
The gross and histologic patterns of abdominal adhesions formed by the use of chromic catgut and polypropylene suture materials were evaluated in dogs. Gross and histologic examinations did not reveal significant difference (p>0.05) in the degree of adhesions observed at enteropexy sites in both suture materials. The use of polypropylene with superior suture qualities should be considered instead of chromic catgut for procedures in which adhesion is desired in the dog.
Tipo: Journal article Palavras-chave: Adhesion; Chromic catgut; Polypropylene; Eneropexy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400037
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In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages Rev. Bras. Ciênc. Avic.
Lee,HY; Biswas,D; Ahn,J.
ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427
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Handling sticky resin by stingless bees (Hymenoptera, Apidae) Rev. Bras. entomol.
Gastauer,Markus; Campos,Lucio A. O.; Wittmann,Dieter.
For their nest defense, stingless bees (Meliponini) collect plant resins which they stick on intruders like ants or cleptobiotic robber bees causing their immobilization. The aim of this article is to identify all parts of stingless bee workers contacting these sticky resins. Of special interest are those body parts with anti-adhesive properties to resin, where it can be removed without residues. For that, extensive behavioral observations during foraging flight, handling and application of the resin have been carried out. When handling the resin, all tarsi touch the resin while walking above it. For transportation from plants to the nest during foraging flight, the resin is packed to the corbicula via tarsi and basitarsi of front and middle legs. Once...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Meliponini; Nest defense; Surface properties.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000200014
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Flocculation of fine fluorite particles with Corynebacterium xerosis Rev. Microbiol.
Haas,Sandra R.; Nascimento,Fábio R.; Schneider,Ivo André H.; Gaylarde,Christine.
The treatment of fine particles dispersed in liquids is common in several industries and especially important in mineral processing. The efficiency of settling operations can be substantially increased by flocculation. The aim of this work was to study the flocculation of fine fluorite particles by the bacterium Corynebacterium xerosis. Flocculation tests, microelectrophoresis measurements and optical microscopy were used to evaluate flocculation. The results showed that C. xerosis cells adhere to the fluorite surfaces promoting the aggregation of the particles. High quality flocs can be obtained rapidly at pH 7.0 using a cell concentration of 40 mg/l, considerably lower than previously reported in the literature. The results are discussed with reference...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Corynebacterium xerosis; Flocculation; Fluorite.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300007
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The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function Anais da ABC (AABC)
Santos,André L.S.; Branquinha,Marta H.; D'Avila-Levy,Claudia M..
Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of Leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosomatidae; Lower trypanosomatids; Gp63; Invertebrate host; Adhesion; Nutrition; Protease.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006
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The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1 Anais da ABC (AABC)
DRAKULIC,DANIJELA; VICENTIC,JELENA MARJANOVIC; SCHWIRTLICH,MARIJA; TOSIC,JELENA; KRSTIC,ALEKSANDAR; KLAJN,ANDRIJANA; STEVANOVIC,MILENA.
The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; HDM2; Migration; P53; SOX2; Wound healing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100389
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Association of number of tandem repeats in two important adhesins in Mycoplasma hyopneumoniae Arq. Bras. Med. Vet. Zootec.
Santos,L. F. dos; Alvarez,J.; Perez,A.; Moreira,M. A. S.; Pieters,M..
RESUMODiversidade genética de Mycoplasma hyopneumoniae tem sido relatada em análise múltipla de repetições em tandem em número variável (MLVA). O objetivo deste estudo foi descrever a distribuição espacial e a heterogeneidade genética de tipos de M. hyopneumoniae no Brasil, bem como investigar a correlação entre regiões de repetição 1 (RR1) e 3 (RR3) de duas adesinas importantes (P97 e P146). Foram identificados 39 tipos de MLVA baseados no número de repetições em tandem em P97 RR1 e RR3 P146. A correlação negativa significativa (Spearman's rho = -0,26; P = 0,022) entre P97 RR1 e RR3 P146 foi observada, o que sugere um possível mecanismo compensatório que permitiria a bactéria manter a sua capacidade de adesão. Os resultados contribuem para compreender a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Adhesion; VNTR; MLVA; Swine; Enzootic pneumonia.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501461
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Regulation of Rho Family GTPases by Cell-Cell and Cell-Matrix Adhesion Biol. Res.
ARTHUR,WILLIAM T.; NOREN,NICOLE K.; BURRIDGE,KEITH.
Integrins and cadherins are transmembrane adhesion receptors that are necessary for cells to interact with the extracellular matrix or adjacent cells, respectively. Integrins and cadherins initiate signaling pathways that modulate the activity of Rho family GTPases. The Rho proteins Cdc42, Rac1, and RhoA regulate the actin cytoskeleton. Cdc42 and Rac1 are primarily involved in the formation of protrusive structures, while RhoA generates myosin-based contractility. Here we examine the differential regulation of RhoA, Cdc42, and Rac1 by integrin and cadherin signaling. Integrin and cadherin signaling leads to a decrease in RhoA activity and activation of Cdc42 and Rac1. When the normal RhoA suppression is antagonized or RhoA signaling is increased, cells...
Tipo: Journal article Palavras-chave: Integrins; Cadherins; Adhesion; Rho; Rac; Cdc42.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200016
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The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro Biol. Res.
Beltran,Sebastian; Munoz-Bergmann,Cristian A; Elola-Lopez,Ana; Quintana,Javiera; Segovia,Cristopher; Trombert,Annette N.
BACKGROUND: Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3:K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases....
Tipo: Journal article Palavras-chave: Lactobacillus; V. parahaemolyticus; Adhesion; Recombinants probiotics.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100002
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In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata) BABT
Balakrishna,Aparna.
The aim of this work was to study the probiotic-related characteristics of four strains of bacteria isolated from the normal flora of the guppy, Poecilia reticulata. In vitro results showed that the strains, namely, MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 had higher adhesion abilities than the tested indicator strains. However, an association between the cell-surface hydrophobicity and the ability to adhere to the intestinal mucus was not observed for these strains. Further, the selected strains were strongly autoaggregating (autoaggregation percentage ≥ 80) and also showed strain-specific coaggregation abilities with the tested indicator strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotics; Adhesion; Aggreggation; Hydrophobicity; Poecilia reticulata.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500010
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Xylitol inhibits J774A.1 macrophage adhesion in vitro BABT
Ferreira,Aline Siqueira; Souza,Maria Aparecida de; Raposo,Nádia Rezende Barbosa; Ferreira,Ana Paula; Silva,Silvio Silvério da.
The aim of this work was to evaluate the effect of xylitol on J774A.1 macrophage adhesion. Adhesion consisted of a three-hour interval, at room temperature, followed by washing and cell incubation at 37ºC/5% CO2/ 48h. Xylitol was used to treat the cells either before (for 24h) or after the cell incubation (for 48h) at 5% as final concentration in both the situations. It was found that xylitol was effective in preventing the adhesion in both the conditions in spite of the former being 100-fold greater and significant (p < 0.001). The results pointed to an important xylitol action on macrophage adhesion, which should be further investigated as an inflammatory control.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; J774A; 1 macrophages; Xylitol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600017
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Influence of gastrointestinal system conditions on adhesion of exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to caco-2 cells BABT
Darilmaz,Derya Onal; Aslım,Belma; Suludere,Zekiye; Akca,Gülcin.
This study aimed to assess the transit tolerance of potential probiotic dairy Lactobacillus strains in human uppergastrointestinal tract in vitro, and to evaluate the effect of EPS production on the viability and adhesion of these strains. Survival and adhesion of two exopolysaccharide (EPS)-producing L. delbrueckii subsp. bulgaricus strains (B3 and B2) and E. coli ATCC11229 were assessed after the exposure of different pH (gastric juice) and gastric plus pancreatic juice challenges. In the artificial gastric juice (pH 2), both the viability of the strain B3 and B2 was decreased. Artificial juice treatments significantly reduced the adhesion to caco-2 cells (P< 0.05). High EPS-producing B3 survived better in the adverse gastrointestinal conditions and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Exopolysaccharide; Caco-2 Cells; Gastric-pancreatic juices; Lactobacillus delbrueckii subsp. bulgaricus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500009
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Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion BJMBR
Zhang,J.P.; Li,N.; Bai,W.Z.; Qiu,X.C.; Ma,B.A.; Zhou,Y.; Fan,Q.Y.; Shan,L.Q..
Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Delta-like 1; Notch signaling; Melanoma; Metastasis; Adhesion; N-cadherin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400299
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Galectins: a key intersection between glycobiology and immunology BJMBR
Rabinovich,G.A.; Riera,C.M.; Landa,C.A.; Sotomayor,C.E..
Galectins are a family of evolutionarily conserved animal lectins, widely distributed from lower invertebrates to mammals. They share sequence and structure similarities in the carbohydrate recognition domain and specificity for polylactosamine-enriched glycoconjugates. In the last few years significant experimental data have been accumulated concerning their participation in different biological processes requiring carbohydrate recognition such as cell adhesion, cell growth regulation, inflammation, immunomodulation, apoptosis and metastasis. In the present review we will discuss some exciting questions and advances in galectin research, highlighting the significance of these proteins in immunological processes and their implications in biomedical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Galectins; Immunomodulation; Apoptosis; Adhesion; Embryogenesis; Metastasis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400002
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Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture BJMBR
Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T..
The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007
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Effect of photodynamic therapy on the extracellular matrix and associated components BJMBR
Pazos,M.d.C.; Nader,H.B..
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002
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CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell BJMBR
Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G..
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004
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Further biochemical characterization of Mycobacterium leprae laminin-binding proteins BJMBR
Marques,M.A.M.; Mahapatra,S.; Sarno,E.N.; Santos,S.; Spencer,J.S.; Brennan,P.J.; Pessolani,M.C.V..
It has been demonstrated that the alpha2 chain of laminin-2 present on the surface of Schwann cells is involved in the process of attachment of Mycobacterium leprae to these cells. Searching for M. leprae laminin-binding molecules, in a previous study we isolated and characterized the cationic proteins histone-like protein (Hlp) and ribosomal proteins S4 and S5 as potential adhesins involved in M. leprae-Schwann cell interaction. Hlp was shown to bind alpha2-laminins and to greatly enhance the attachment of mycobacteria to ST88-14 Schwann cells. In the present study, we investigated the laminin-binding capacity of the ribosomal proteins S4 and S5. The genes coding for these proteins were PCR amplified and their recombinant products were shown to bind...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium leprae; Laminin; Adhesion; Schwann cell; Ribosomal protein; Histone.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400004
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Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins BJMBR
Lima,O.C.; Figueiredo,C.C.; Pereira,B.A.S.; Coelho,M.G.P.; Morandi,V.; Lopes-Bezerra,L.M..
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sporothrix schenckii; Fibronectin; Laminin; Type II collagen; Adhesion.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500020
Registros recuperados: 53
Primeira ... 123 ... Última
 

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