|
|
|
| Registros recuperados: 47 | |
|
|
| Cunha,E.M.S.; Nassar,A.F.C.; Lara,M.C.C.S.H.; Bersano,J.G.; Villalobos,E.M.C.; Oliveira,J.C.F.. |
| Serum samples collected from 358 wild boars (Sus scrofa) in breeding farms in São Paulo, southeast Brazil, from 1998 to 2001, were tested for antibodies against pseudorabies virus (PRV) by means of serum neutralization (SN) and enzyme-linked immunobsorbent assay (ELISA). Seropositive animals were detected in three of seven herds analyzed. Overall seroprevalence as assessed by SN was 30.7%, ranging from 25.2% to 100% for the herds that presented seropositive animals. Indirect ELISA detected lower seroprevalence (19.3%). Sensitivity and specificity of ELISA were equal to 57.3% and 97.6%, respectively. Agreement was equal to 85.2% (P<0.0001). These results showed that PRV infections occurred in farmed feral swine in southeast Brazil, and affect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wild boar; Sus scrofa; Pseudorabies virus; Aujeszkys disease virus; Antibodies. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400002 |
| |
|
| |
|
|
| Passos,L.M.F.; Ribeiro,M.F.B.; Anderegg,P.I.; Böse,R.. |
| O objetivo do presente estudo foi determinar a dinâmica da produção de anticorpos anti-Babesia equi e B. caballi em éguas gestantes em condições naturais de campo em Minas Gerais e avaliar a eficiência da transferência passiva de anticorpos via colostro. Foram analisadas, pela imunofluorescência indireta, amostras de soros colhidas de 34 fêmeas aos 290, 305 e 320 dias de gestação, e no momento do parto, e de seus respectivos potros até 36 horas após o nascimento. Aos 290 dias de gestação, todas as fêmeas estavam positivas para B. equi e apenas duas estavam negativas para B. caballi. Todos os potros tornaram-se positivos para B. equi após ingestão de colostro, entretanto 42% permaneceram negativos para B. caballi 36 horas após o nascimento. Não se observou... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Babesia equi; Babesia caballi; Pregnant mares; Antibodies. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600003 |
| |
|
|
| Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos. |
| In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010 |
| |
|
| |
|
| |
|
|
| Nunes,Cristina Freitas; Fonseca,Fabiane; Leite,Alice Teixeira Meirelles; Silva Filho,Rodolfo Pinho da; Finger,Paula Fonseca; Castro,Clarissa Caetano; Fischer,Geferson; Vargas,Gilberto D'avila; Hübner,Silvia de Oliveira. |
| To investigate the exposure of the Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian poxvirus (APV) in Magellanic penguins found on the beaches in Southern regions of Brazil, the frequency of serum antibodies was estimated in 89 samples taken during 2005 and 2006. All the penguins were negative for the presence of antibodies against NDV by hemagglutination inhibition test and to APV by indirect ELISA. The reactivity was similar to the positives controls using ELISA kit for the IBDV made in the chickens in 50 samples. This reactivity also was demonstrated in 42 samples using agar gel immunodiffusion. No clinical signs related to IBDV infection were observed. The results indicated the absence of infection by NDV and APV but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Magellanic penguins; Antibodies; Newcastle disease virus; Infectious bursal disease virus; Avian poxvirus. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400008 |
| |
|
|
| Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio. |
| Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003 |
| |
|
| |
|
|
| Barone,Antonio Alci; Tosta,Rose Aparecida Borges; Tengan,Fátima Mitiko; Marins,José Humberto Caetano; Cavalheiro,Norma de Paula; Cardi,Bruno Andrade. |
| A follow-up study was made of 94 chronic hepatitis C patients at a hepatitis clinic in Brazil, after interferon alpha (IFN-alpha) therapy, to determine the influence of anti-interferon antibodies on treatment outcome. Patients diagnosed as having chronic hepatitis C, confirmed by PCR (HCV RNA) and liver biopsy, were treated with interferon alpha 2a or 2b for at least six months, and were followed up for 24 weeks after termination of treatment in order to assess biochemical, virological and clinical pathology responses. Only 6% of the 94 patients developed anti-IFN antibodies, 70% presented a biochemical response and 23% maintained a sustained virological response. Clinical evaluation revealed that in only 2 patients was there progression of fibrosis; the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Interferon-alpha; Antibodies; Chronic HCV; Treatment. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100002 |
| |
|
| |
|
| |
|
| |
|
|
| Rodríguez,Islay; Fernández,Carmen; Sánchez,Lizet; Martínez,Bárbara; Siegrist,Hans H.; Lienhard,Reto. |
| Lyme disease has not been officially reported in Cuba. However, clinical cases have been serologically reported. Seroprevalence survey of Borrelia burgdorferi sensu stricto antibodies in humans in the country has not been conducted. OBJECTIVE: To estimate the prevalence of borrelial antibodies in inhabitants of a village with historically high level of tick infestation. METHODS: Serum specimens from 247 persons randomly selected from the population of the village were examined by IgG Western blot using B31 strain for estimating the prevalence of antibodies profile. RESULTS: A seroprevalence value interval (95% CI) of 0.6%-7.2% was estimated for the studied population. The prevalent borrelial protein bands on immunoblots were 41, 72, 90/93, 34, 47, 60, 58,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Borrelia burgdorferi; Prevalence; Lyme disease; Antibodies. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100015 |
| |
|
|
| Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo. |
| S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a 23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of antibodies found only against... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005 |
| |
|
| |
|
|
| Kiffer,Carlos Roberto Veiga; Conceição,Orlando Jorge; Santos,Edgar Bortholi; Sabino,Ester; Focaccia,Roberto. |
| OBJECTIVES: Estimate the prevalence of immunity to poliomyelitis (anti-polio antibodies) in the city of São Paulo/Brazil through a population-based survey. METHODS: A quantitative and inductive method was used to draw a representative sample of the population. Randomization and stratification (based on sex, age and residence region) was done, and 1,059 individuals were studied on a home-visit basis (structured questionnaires and blood samples). A microneutralization test was performed to detect anti-polio antibodies against serotypes 1, 2 and 3. RESULTS: The estimated prevalence of immunity to poliomyelitis was high, with 94.6% prevalence of anti-polio 1 antibodies, 98.8% anti-polio 2 and 91.9% anti-polio 3. Despite this high prevalence, there were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunity prevalence; Poliomyelitis; Poliviruses; Seroepidemiologic studies; Antibodies; Viral blood. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500004 |
| |
|
|
| dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.. |
| Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616 |
| |
|
|
| Veloso,D.. |
| Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 <FONT FACE="Symbol">®</FONT> stop) has been blamed for the absence of LK and HK. However, using Western blots... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004 |
| |
|
|
| Ouverney,E.P.; Teixeira,S.L.M.; Silva-de-Jesus,C.; Hacker,M.A.; Morgado,M.G.; Bastos,F.I.; Bongertz,V.. |
| Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Injecting drug users; Antibodies; Neutralization. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900004 |
| |
| Registros recuperados: 47 | |
|
|
|
