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Biodegradation and toxicological evaluation of lubricant oils BABT
Tamada,Ivo Shodji; Lopes,Paulo Renato Matos; Montagnolli,Renato Nallin; Bidoia,Ederio Dino.
The aim of this work was to compare different toxicity levels of lubricant oils. The tests were performed using the earthworm (Eisenia andrei), arugula seeds (Eruca sativa) and lettuce seeds (Lactuca sativa), with three types of contaminants (mineral lubricant oil, synthetic lubricant oil and used lubricant oil) for various biodegradation periods in the soil. The toxicity tests indirectly measured the biodegradation of the contaminants. The samples were analyzed at t0, t60, t120 and t180 days of biodegradation. The used lubricant oil was proved very toxic in all the tests and even after biodegradation its toxicity was high. The mineral and synthetic oils were biodegraded efficiently in the soil although their toxicity did not disappear completely after 180...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxicology; Biodegradation; Lubricant oil; Toxicity bioassay.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600020
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Anti-Fusarium moniliforme activity and fumonisin biodegradation by corn and silage microflora BABT
Camilo,Simone B.; Ono,Carlos José; Ueno,Yoshio; Hirooka,Elisa Y..
Studies were carried out to isolate microorganisms from corn and silage screened for their ability to inhibit F. moniliforme growth (strain 113F) in association with fumonisin detoxification. Among 150 isolates four Gram-positive bacilli and one yeast with inhibitory activity were selected. The inhibition zone ranged from 50 to 72.5 mm using cultures, and from 25 to 52.5mm for crude alcoholic extract. The isolates S9, S10, S69 (sporulated bacilli) and SE3071 (yeast) degraded 43, 48, 83 and 57% of the initial FB1 concentration, respectively. The pH increased gradually in the medium during incubation for biodegradation process.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium moniliforme; Fumonisin; Biodegradation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200004
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Effect of biologically treated petroleum sludge on seed germination and seedling growth of Vigna unguiculata (L.) Walp. (Fabaceae) BABT
Sangeetha,Jeyabalan; Thangadurai,Devarajan.
The present investigation was carried out to study the response of different concentrations of treated petroleum sludge on seed germination, root and shoot length and tolerance of Vigna unguiculata (L.) Walp. The biologically treated petroleum sludge with bacterial consortium showed 54.8% reduction in total petroleum hydrocarbons. Treated sludge was utilized with agricultural soil in known concentration for the assessment of growth of V. unguiculata. A remarkable absence of seed germination was observed at higher sludge concentration. The different concentrations of treated petroleum sludge showed severe decline on the length, weight and vigour index of the tested seedlings with increasing sludge concentrations. The results showed that the difference in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial consortium; Biodegradation; Petroleum sludge; Seed germination; Total petroleum hydrocarbon; Vigna unguiculata.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300017
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Biodegradation of stored jet fuel by a Nocardia sp. isolated from contaminated soil BABT
Gomes,Edelvio de Barros; Soriano,Adriana Ururahy; Miranda,Rita de Cássia Mendonça de; Sousa,Maria de Fátima Vieira de Queiroz; Pereira Jr.,Nei.
The aim of this study was to investigate the potential of degradation of an autochthonous bacterial strain, isolated from petroleum derivatives contaminated soil samples against jet fuel hydrocarbons. The autochthonous bacterial strain was characterized as Nocardia sp. Evaluation of their degrading abilities was carried out by presumptive assays as redox indicator test and by observations of surface tension decreases in aqueous medium. Degradation of jet fuel hydrocarbons was evaluated by chromatographic methods. Experiments were performed in flasks at two biostimulation rates. A bacterial strain of Pseudomonas aeruginosa UFPEDA 39 was utilized as a reference microorganism. The bacterial strain, identified as Nocardia sp, demonstrate high ability to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Nocardia; Jet fuel.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500027
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Biodegradation of erythrosin B dye by paramorphic Neurospora crassa 74A BABT
Jesus,Gisele Jane de; Corso,Carlos Renato; Campos,Adriana de; Franchetti,Sandra Mara Martins.
The present work used paramorphic forms of Neurospora crassa 74A to remove erythrosine. The fungus culture was grown in medium containing the dye, as only carbon source for 2 and 90 h of interaction. A washing process using distilled water isolated the cellular mass mycelia was dried for 12 h at 105ºC and transformed in fine powder and analyzed in FTIR. The supernatant was analyzed through spectrophotometer UV-Vis and FTIR. Significant differences in the spectrum of UV-VIS and FTIR were observed between the control and the supernatant and between wall control and the walls colored by red, in FTIR for 2 and 90 h. Some significant bands were modified, suggesting the possibility of enzymatic biodegradation in proportion to the time of contact between the dye...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; FTIR; Neurospora crassa; Paramorphic; Erythrosin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200028
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Evaluation of the biodegradation of different types of lubricant oils in liquid medium BABT
Lopes,Paulo Renato Matos; Bidoia,Ederio Dino.
The aim of this work was to study the biodegradation of different types of automotive lubricant oils adapted to the aqueous medium using a base inoculum and an aqueous inoculum. Four treatments were carried out in two consecutive and similar experiments: T1 (control); T2 (half-synthetic oil); T3 (mineral oil); T4 (used oil). The results showed the following decreasing order of CO2 production in the Bartha and Pramer respirometers: T4 > T2 > T3 > T1. Thus, the used lubricant oil showed with highest biodegradability, followed by the half-synthetic one and the mineral oil. It was also observed that the mineral lubricant presented a longer period of adaptation compared to the half-synthetic one.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Lubricant oil; Respirometry; Liquid medium.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500028
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Biodegradation of oil refinery residues using mixed-culture of microorganisms isolated from a landfarming BABT
Morais,Eduardo Beraldo de; Tauk-Tornisielo,Sâmia Maria.
In this study, the potential for using an inoculum composed of a mixed-culture of bacteria and fungi, isolated from a landfarming at the Paulínia Oil Refinery, Brazil, to degrade oil residues generated in the process of petroleum refinement was investigated. The isolation of these microorganisms was carried out beforehand, assuming that they would be better adapted to petroleum hydrocarbons, as the landfarming consisted of an area impacted by the deposit of such compounds. The Bartha and Pramer respirometric test was used to measure the rate of biodegradation of the hydrocarbons by the mixed-culture of microorganisms via the evolution of CO2. The results obtained with respect to the efficiency of biodegradation showed no significant differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Oil residue; Petroleum; Inoculum; Mixed-culture.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600029
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Start-up and steady-state conditions of an Anaerobic Hybrid Reactor (AHR) using mini-filters composed with two types of support medium operating under low loading rates BABT
Silva,Vivian Galdino da; Campos,Cláudio Milton Montenegro; Pereira,Erlon Lopes; Silva,Júlia Ferreira da.
The present work aimed at studying the operational parameters of an Anaerobic Hybrid Reactor (AHR) removing organic matter of coffee wastewater with low concentration. The AHR was built similar to an UASB reactor, however the interior was filled with mini-filters composed by two types of support materials: expanded clay and rolled pebble. Three start-ups were accomplished in order to achieve the stationary state (steady-state). Three hydraulic retention times were appraised: 28.5; 24.0 and 18.0 h, obtaining a volumetric loading rate (VLR) of 0.70; 0.56; 0.54 kg COD m-3 d-1 and a biological organic loading rate (BOLR) of 0.0156; 0.0103 and 0.01213 kg BOD5 kg TVS-1 d-1. Due to the decrease in the concentration of organic matter in the influent, the endogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental chemistry; Coffee wastewater; Biodegradation; Optimization process.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500015
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Properties of catechol 1,2-dioxygenase in the cell free extract and immobilized extract of Mycobacterium fortuitum BJM
Silva,A.S.; Jacques,R.J.S.; Andreazza,R.; Bento,F.M.; Roesch,L.F.W.; Camargo,F.A.O..
Polycyclic aromatic hydrocarbons (PAH) are carcinogenic compounds which contaminate water and soil, and the enzymes can be used for bioremediation of these environments. This study aimed to evaluate some environmental conditions that affect the production and activity of the catechol 1,2-dioxygenase (C12O) by Mycobacterium fortuitum in the cell free and immobilized extract in sodium alginate. The bacterium was grown in mineral medium and LB broth containing 250 mg L-1 of anthracene (PAH). The optimum conditions of pH (4.0-9.0), temperature (5-70 ºC), reaction time (10-90 min) and the effect of ions in the enzyme activity were determined. The Mycobacterium cultivated in LB shown higher growth and the C12O activity was two-fold higher to that in the mineral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracene; Enzyme activity; Enzyme immobilization; Biodegradation; Waste treatment.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100043
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Isolation and preliminary characterization of a o-nitrobenzaldehyde-degrading Alcaligenes sp. ND1 BJM
Fang-Bo,Yu; Li-Bo,Guan; Shan,Zhou.
This paper reports the isolation and characterization of a new o-nitrobenzaldehyde (ONBA)-degrading bacterium, Alcaligenes sp. ND1. ND1 degraded almost all ONBA (100 mg L-1) in M9 medium within 36 hours. The key enzyme(s) involved in the initial biodegradation was a constitutively intracellular enzyme(s). This bacterium has great potential utility for bioremediation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; O-nitrobenzaldehyde.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400033
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Degradation of h-acid by free and immobilized cells of Alcaligenes latus BJM
Usha,M.S.; Sanjay,M.K.; Gaddad,S.M.; Shivannavar,C.T..
Alcaligenes latus, isolated from industrial effluent, was able to grow in mineral salts medium with 50 ppm (0.15 mM) of H-acid as a sole source of carbon. Immobilization of Alcaligenes latus in Ca-alginate and polyurethane foam resulted in cells embedded in the matrices. When free cells and immobilized cells were used for biodegradation studies at concentration ranging from 100 ppm (0.3 mM) to 500 ppm (1.15 mM) degradation rate was enhanced with immobilized cells. Cells immobilized in polyurethane foam showed 100% degradation up to 350 ppm (1.05 mM) and 57% degradation at 500 ppm (1.5 mM). Degradation rate of Ca-alginate immobilized cells was less as compared to that of polyurethane foam immobilized cells. With Ca-alginate immobilized cells 100%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: H-acid; Biodegradation; Immobilized cells; Alcaligenes latus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400012
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Best conditions for biodegradation of diesel oil by chemometric tools BJM
Kaczorek,Ewa; Bielicka-Daszkiewicz,Katarzyna; Héberger,Károly; Kemény,Sándor; Olszanowski,Andrzej; Voelkel,Adam.
Diesel oil biodegradation by different bacteria-yeast-rhamnolipids consortia was tested. Chromatographic analysis of post-biodegradation residue was completed with chemometric tools (ANOVA, and a novel ranking procedure based on the sum of ranking differences). These tools were used in the selection of the most effective systems. The best results of aliphatic fractions of diesel oil biodegradation were observed for a yeast consortia with Aeromonas hydrophila KR4. For these systems the positive effect of rhamnolipids on hydrocarbon biodegradation was observed. However, rhamnolipids addition did not always have a positive influence on the biodegradation process (e.g. in case of yeast consortia with Stenotrophomonas maltophila KR7). Moreover, particular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Chemometrics; Systems ranking; Variance analysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100017
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Thiocyanate degradation by pure and mixed cultures of microorganisms BJM
Souza-Fagundes,Elaine M.; Rosa,Luiz H.; Gomes,Newton C.M.; Santos,Mércia H.; Pimentel,Patricia F..
A mixed culture and a pure bacterial strain (BMV8) were isolated from a bioreactor for thiocyanate treatment. Both cultures removed 5 mM of thiocyanate from the medium in 36 hours. The mixed culture was able to tolerate concentrations up to 60 mM. The efficiency of thiocyanate degradation decreased when the cells were immobilized.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Cyanate; Immobilization; Microorganisms; Thiocyanate.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300012
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GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium BJM
Luo,Qun; He,Ying; Hou,Deng-Yong; Zhang,Jian-Guo; Shen,Xian-Rong.
To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkane hydroxylase; Expression; Escherichia coli DH5α; Biodegradation; Diesel oil.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300649
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Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp: ONBA-17 and deduction on its metabolic pathway BJM
Yu,Fang-Bo; Li,Xiao-Dan; Ali,Shinawar Waseem; Shan,Sheng-Dao; Luo,Lin-Ping; Guan,Li-Bo.
A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas putida; Biodegradation; Metabolic pathway; Nitroaromatics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400021
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Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852 BJM
Otenio,Marcelo Henrique; Silva,Maria Teresa Lopes da; Marques,Maria Luiza Oliveira; Roseiro,José Carlos; Bidoia,Ederio Dino.
A minimal liquid medium containing benzene (B), toluene (T) and xylene (X) and mixtures thereof, was used to evaluate degradation activity of Pseudomonas putida CCMI 852 containing a TOL plasmid. Experiments were developed with B, T and X (100 mg L-1), with mixtures of BT, BX, and TX (50 + 50 mg L-1 each) and BTX (33.3 + 33.3 + 33.3 mg L-1 each), added to 500 mL of medium. After 18 to 24 hours, the inoculum was added and solvent disappearance was determined after 24 to 25 hours by GC. Results showed that P. putida CCMI 852 was able to metabolize T and X, but B was not metabolized. In a BTX mixture, B was not metabolized and T and X degradation rate decreased 50%.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas putida; Biodegradation; BTX; Plasmid TOL.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300010
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Carbaryl degradation by bacterial isolates from a soil ecosystem of the Gaza Strip BJM
Hamada,Mazen; Matar,Ammar; Bashir,Abdallah.
Abstract Carbaryl is an important and widely used insecticide that pollutes soil and water systems. Bacteria from the local soil ecosystem of the Gaza Strip capable of utilizing carbaryl as the sole source of carbon and nitrogen were isolated and identified as belonging to Bacillus, Morganella, Pseudomonas, Aeromonas and Corynebacterium genera. Carbaryl biodegradation by Bacillus, Morganella and Corynebacterium isolates was analyzed in minimal liquid media supplemented with carbaryl as the only source of carbon and nitrogen. Bacillus and Morganella exhibited 94.6% and 87.3% carbaryl degradation, respectively, while Corynebacterium showed only moderate carbaryl degradation at 48.8%. These results indicate that bacterial isolates from a local soil ecosystem...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Soil bacterial isolates; Carbaryl; TLC.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401087
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Isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of Zarand, Kerman BJM
Karimi,Maryam; Hassanshahian,Mehdi.
Abstract Phenol and phenolic compounds are environmental pollutants present in industrial wastewaters such as coal tar, oil refineries and petrochemical plants. Phenol removal from industrial effluents is extremely important for the protection of environment. Usually, phenol degradation is carried out by physicochemical methods that are costly and produce hazardous metabolites. Recently, phenol biodegradation has been considered. Yeasts are the most important phenol biodegraders. In this study, the phenol-degrading yeast from environmental samples (soil and wastewater) was isolated from the coking plant of Zarand, Kerman. Then total heterotrophic yeasts were counted. The soil samples had higher rates of yeast degrader, in comparison to wastewater samples....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Coal tar; Coking plant; Yeast; Zarand.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100018
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Biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoides BJM
Bisht,Sandeep; Pandey,Piyush; Sood,Anchal; Sharma,Shivesh; Bisht,N. S..
Several naphthalene and anthracene degrading bacteria were isolated from rhizosphere of Populus deltoides, which were growing in non-contaminated soil. Among these, four isolates, i.e. Kurthia sp., Micrococcus varians, Deinococcus radiodurans and Bacillus circulans utilized chrysene, benzene, toluene and xylene, in addition to anthracene and naphthalene. Kurthia sp and B. circulans showed positive chemotactic response for naphthalene and anthracene. The mean growth rate constant (K) of isolates were found to increase with successive increase in substrate concentration (0.5 to 1.0 mg/50ml). B. circulans SBA12 and Kurthia SBA4 degraded 87.5% and 86.6% of anthracene while, Kurthia sp. SBA4, B. circulans SBA12, and M. varians SBA8 degraded 85.3 %, 95.8 % and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Chemotaxis; Anthracene; Naphthalene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400011
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CULTIVATION OF THE EDIBLE MUSHROOM OUDEMANSIELLA CANARII (JUNGH.) HÖHN. IN LIGNOCELLULOSIC SUBSTRATES BJM
Ruegger,Marcelo José Silveira; Tornisielo,Sâmia Maria Tauk; Bononi,Vera Lúcia Ramos; Capelari,Marina.
The edible mushroom Oudemansiella canarii (Jungh.) Höhn is common in the Brazilian territory, being found in different biomas, where they colonize several plant species. In this study, the O. canarii cultivation was evaluated in polypropilene bags containing sugar-cane bagasse (200 g) or eucalyptus sawdust (200 g) supplemented with wheat bran (50 g). The composts were sterilized at 121ºC for 1 hour, after cooling they were inoculated with 3 g of spawn and then remained incubated at 25ºC until the basidiomata primordia formation. The mushrooms, harvested after the pilei opening, presented varied sizes reaching 9 cm of diameter and 10 cm of height. The fresh mushrooms presented mild taste and soft consistency. When kept at 4ºC, they maintained good...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oudemansiella canarii; Basidiomycetes; Mushroom; Solid substrates; Biodegradation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300009
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