SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Bala, K.; Robideau, G.P.; Désaulniers, N.; Cock, A.W.A.M. de; Lévesque, C.A..

Three new species of Pythium, namely, P. oopapillum, P. emineosum and P. camurandrum are presented in this paper based on morphological descriptions and molecular phylogenetic characterisation. These new species were isolated from various ecological regions in Canada. They have unique morphological features in the genus Pythium, and form distinct clades in maximum parsimony analyses, which are also supported by maximum likelihood phylogeny using general time reversible model (GTR), and Bayesian inference (BI) phylogeny using Markov Chain Monte Carlo (MCMC) analysis methods. A comparative study of the new species with closely related taxa, their clade positions, and morphological features are described in this paper.

Antispila oinophylla new species (Lepidoptera, Heliozelidae), a new North American grapevine leafminer invading Italian vineyards: taxonomy, DNA barcodes and life cycle

Palavras-chave: COI; ITS rDNA; LSU rDNA; Oomycete; Peronosporales; Pythium; Taxonomy.

Ano: 2010

URL: http://www.repository.naturalis.nl/record/531810

Nieukerken, E. van; Wagner, D.; Baldessari, M.; Mazzon, L.; Angeli, G.; Girolami, V.; Duso, C.; Doorenweerd, C..

A grapevine leafminer Antispila oinophylla van Nieukerken & Wagner, sp. n., is described both from eastern North America (type locality: Georgia) and as a new important invader in North Italian vineyards (Trentino and Veneto Region) since 2006. The species is closely related to, and previously confused with A. ampelopsifoliella Chambers, 1874, a species feeding on Virginia creeper Parthenocissus quinquefolia (L.) Planchon., and both are placed in an informal A. ampelopsifoliella group. Wing pattern, genitalia, and DNA barcode data all confirm the conspecificity of native North American populations and Italian populations. COI barcodes differ by only 0–1.23%, indicating that the Italian populations are recently established from eastern North America....

Genetic and morphological variation in corallivorous snails (Coralliophila spp.) living on different host corals at Curaçao, southern Caribbean

Palavras-chave: Invasive species; New species; Vitaceae; Viticulture; COI; Leafmines; Venation; Genitalia; Holocacista rivillei; 42.75.

Ano: 2012

URL: http://www.repository.naturalis.nl/record/411010

Potkamp, G.; Vermeij, M.J.A.; Hoeksema, B.W..

Snails of the genus Coralliophila (Muricidae: Coralliophilinae) are common corallivores in the Caribbean, feeding on a wide range of host species. In the present study, the morphological and genetic variation in C. galea and C. caribaea were studied in relation to their association with host coral species at Curaçao. Differences in shell shape among snails living on different hosts were quantified using geometric morphometric and phylogenetic relationships were studied using two mitochondrial markers (12S and COI). Based on these analyses, a new species, C. curacaoensis sp. nov., was found in association with the scleractinian coral Madracis auretenra. Both C. galea and C. caribaea showed host-specific differences in shell shape, size, and shell allometry...

A molecularly based phylogeny reconstruction of mushroom corals (Scleractinia: Fungiidae) with taxonomic consequences and evolutionary implications for life history traits

Palavras-chave: Genetic differentiation; Phylogenetics; 12S; COI; Corallivory; Morphology; Geometric morphometrics; Host race.

Ano: 2017

URL: http://www.repository.naturalis.nl/record/630190

Gittenberger, A.; Reijnen, B.T.; Hoeksema, B.W..

The phylogenetic relationships of the Fungiidae, a family of predominantly free-living, zooxanthellate, reef corals, were studied by sequencing a part of the mitochondrial Cytochrome Oxidase I (COI) and the complete ribosomal Internal Transcribed Spacers (ITS) I & II of specimens from various locations in the Indo- West Pacific. Some sequences were retrieved by using fungiidspecific primers on DNA-extracts from parasitic gastropods living with these corals. The analyses were performed both including and excluding intraspecific variation to investigate the potential effect of saturation. Even though the present molecular phylogeny reconstructions largely reflect those based on morphological characters, there are some distinct differences. Three major...

Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida: Isopoda) systematics

Palavras-chave: COI; Evolutionary history; ITS 1 & 2; Maximum coral size; Polystomatism; Reproduction strategy; 42.72.

Ano: 2011

URL: http://www.repository.naturalis.nl/record/376596

Wetzer, R..

Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...

Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home

Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.

Ano: 2001

URL: http://www.repository.naturalis.nl/record/534397

Benzoni, F.; Arrigoni, R.; Stefani, F.; Reijnen, B.T.; Montano, S.; Hoeksema, B..

The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...

Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)

Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA; 42.72.

Ano: 2012

URL: http://www.repository.naturalis.nl/record/421558

Arrigoni, R.; Richards, Z.T.; Chen, C.A.; Baird, A.H.; Benzoni, F..

Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...

Phytopythium: molecular phylogeny and systematics

Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision; 42.72.

Ano: 2014

URL: http://www.repository.naturalis.nl/record/499033

Cock, A.W.A.M. de; Lodhi, A.M.; Rintoul, T.L.; Bala, K.; Robideau, G.P.; Gloria Abad, Z.; Coffey, M.D.; Shahzad, S.; Lévesque, C.A..

The genus Phytopythium (Peronosporales) has been described, but a complete circumscription has not yet been presented. In the present paper we provide molecular-based evidence that members of Pythium clade K as described by Lévesque & de Cock (2004) belong to Phytopythium. Maximum likelihood and Bayesian phylogenetic analysis of the nuclear ribosomal DNA (LSU and SSU) and mitochondrial DNA cytochrome oxidase subunit 1 (COI) as well as statistical analyses of pairwise distances strongly support the status of Phytopythium as a separate phylogenetic entity. Phytopythium is morphologically intermediate between the genera Phytophthora and Pythium. It is unique in having papillate, internally proliferating sporangia and cylindrical or lobate antheridia. The...

The enigmatic Marmorkrebs (marbled crayfish) is the parthenogenetic form of Procambarus fallax (Hagen, 1870)

Palavras-chave: COI; LSU; Oomycetes; Oomycota; Peronosporales; Phytopythium; Pythiales; SSU.

Ano: 2015

URL: http://www.repository.naturalis.nl/record/560055

Martin, P.; Dorn, N.J.; Kawai, T.; Heiden, C. van der; Scholtz, G..

A mysterious parthenogenetic cambarid crayfish (the Marmorkrebs) has been spreading across the globe for the past decade. We compare this crayfish directly to two other cambarids, Procambarus fallax and P. alleni, that have been suggested to be related or even identical to the Marmorkrebs. Using external morphology and sequences of two mitochondrial genes we show clear correspondences between Marmorkrebs and P. fallax, a species found natively throughout peninsular Florida, USA. Based on these congruent results we suggest that the Marmorkrebs is the parthenogenetic form of P. fallax. This finding has potential evolutionary and ecological implications at several levels. The Marmorkrebs might be a type of geographical parthenogenesis, but a natural...

Phylogenetic Relationships Among Species of the fraterculus Group (Anastrepha: Diptera: Tephritidae) Inferred from DNA Sequences of Mitochondrial Cytochrome Oxidase I

Palavras-chave: 12S rRNA; Annulus ventralis; COI; DNA barcoding; Species concept; Thelytoky; 42.74.

Ano: 2010

URL: http://www.repository.naturalis.nl/record/355212

Neotropical Entomology

SMITH-CALDAS,MARTHA R.B.; MCPHERON,BRUCE A.; SILVA,JANISETE G.; ZUCCHI,ROBERTO A..

A fragment of 808 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analyzed for 15 species of Anastrepha: 12 within the fraterculus group, one unplaced species and two outgroups. Phylogenetic relationships among the included taxa were inferred using neighbor-joining and maximum parsimony methods. The average Jukes-Cantor genetic distance among the species was 0.033±0.006 and the level of sequence divergence ranged from 0.0 to 0.083. Our results of COI indicate the placement of A. acris Stone, an unplaced species, in the fraterculus group. The membership of A. barbiellinii Lima in the fraterculus group and the monophyly of the aforementioned group are also discussed. Moreover, the presence of multiple gene pools in the nominal species A....

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Insecta; Fruit flies; Anastrepha fraterculus; COI.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000400009

Molecules, wing pattern and distribution: an approach to species delimitation in the "loxurina group" (Lepidoptera: Lycaenidae: Penaincisalia)

Neotropical Entomology

Prieto,C; Micó,E; Galante,E.

The wide range of morphological variations in the "loxurina group" makes taxa identification difficult, and despite several reviews, serious taxonomical confusion remains. We make use of DNA data in conjunction with morphological appearance and available information on species distribution to delimit the boundaries of the "loxurina" group species previously established based on morphology. A fragment of 635 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analysed for seven species of the "loxurina group". Phylogenetic relationships among the included taxa were inferred using maximum parsimony and maximum likelihood methods. Penaincisalia sigsiga (Bálint et al), P. cillutincarae (Draudt), P. atymna (Hewitson) and P. loxurina (C. Felder...

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin

Palavras-chave: COI; Eumaeini; Thecloxurina; Andes; Butterfly.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000500006

Ribolli,Josiane; Scaranto,Bianca Maria; Shibatta,Oscar Akio; Bombardelli,Robie Allan; Zaniboni-Filho,Evoy.

ABSTRACT DNA barcoding is a widely utilized molecular-based identification of species and taxonomic resolutions. Until recently, Rhamdia voulezi and Rhamdia branneri were considered species synonyms of Rhamdia quelen; however, morphological and cytogenetic analyses have suggested the validity of distinct species. Due to the absence of molecular taxonomy of R. voulezi and R. branneri, the objective of this study was to test its validity through traditional DNA barcoding and the GMYC (General Mixed Yule Coalescent) COI-based analyses in 19 specimens from the Iguaçu River Basin. In both methodologies, three MOTUs (Molecular Operational Taxonomic Units) were identified based on the estimated optimum threshold (OT = 0.77). The average inter-MOTU distance (NJ,...

Cytogenetic and DNA barcoding reveals high divergence within the trahira, Hoplias malabaricus (Characiformes: Erythrinidae) from the lower Amazon River

Palavras-chave: Aquaculture; COI; Jundiá; MOTU; Species complex.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100217

Marques,Diego Ferreira; Santos,Fabíola Araújo dos; Silva,Simoni Santos da; Sampaio,Iracilda; Rodrigues,Luís Reginaldo Ribeiro.

Molecular and cytogenetic data have provided evidence of cryptic speciation in the widespread South American trahira, Hoplias malabaricus. In the present study, karyotypes and DNA barcode sequences of specimens from seven populations inhabiting the lower Amazon River were analyzed in order to characterize the levels of genetic divergence within a single karyomorph. All the specimens presented karyotypes with 2n = 40 chromosomes (20m+20sm) that were consistent with the species' C karyomorph. The DNA barcodes revealed six haplogroups, with clear divergence between populations from Brazil and Argentina. The results support the species complex hypothesis and indicate that a single karyomorph of H. malabaricus may harbor more than one species

Phylogeography and conservation genetics of the Amazonian freshwater stingray Paratrygon aiereba Müller & Henle, 1841 (Chondrichthyes: Potamotrygonidae)

Palavras-chave: Amazon basin; COI; Karyomorph; Species complex; Trahiras.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200459

Frederico,Renata G.; Farias,Izeni P.; Araújo,Maria Lúcia Góes de; Charvet-Almeida,Patricia; Alves-Gomes,José A..

The family Potamotrygonidae is monophyletic comprising three genera: Paratrygon Duméril, Potamotrygon Garman and Plesiotrygon Rosa, Castello & Thorson. The distribution of most species in this family is restricted to a single basin or fluvial system. Only Potamotrygon motoro, Potamotrygon orbignyi and Paratrygon aiereba are found in more than one river basin. In this study we investigate genetic structuring of Paratrygon aiereba, from five rivers of the Amazon region: Negro, Solimões-Amazon-Estuary system, Tapajós, Xingu and Araguaia. Sixty-three individuals were sequenced for ATPase 6, and a representative subsample of 27 individuals was sequenced for COI. The COI dataset analysis indicated that Paratrygon is sister to all other potamotrygonid...

Morphometric variation of the Herichthys bartoni (Bean, 1892) species group (Teleostei: Cichlidae): How many species comprise H. labridens (Pellegrin, 1903)?

Palavras-chave: Amazon basin; ATPase; COI; Population genetics.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100007

Mejía,Omar; Pérez-Miranda,Fabián; León-Romero,Yatzil; Soto-Galera,Eduardo; Luna,Efraín de.

Cichlids of the tribe Heroini have long been a source of taxonomical conflict. In particular, the species included in the Herichthys bartoni group have failed to be recovered as monophyletic in different molecular studies. In this paper we use traditional and geometric morphometrics to evaluate morphological variation in the species included in the H. bartoni complex in order to evaluate the number of species it contains. An update of a previously published DNA barcoding study suggests the existence of three genetic clusters that included the six recognized species analyzed in this study, none of them recovered as monophyletic. On the other hand, geometric morphometrics arise as a useful tool to discriminate species due that traditional morphometrics...

Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family

Palavras-chave: Cichlids; COI; DNA barcode; Geometric morphometrics; Mexico.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100061

Hett,Anne Kathrin; Nirchio,Mauro; Oliveira,Claudio; Siccha,Zoila Raquel; Rossi,Anna Rita; Sola,Luciana.

This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA...

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

Palavras-chave: C-banding; COI; 5S rDNA; Mullet; NORs.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100008

Damasceno,Júnio S.; Siccha-Ramirez,Raquel; Oliveira,Claudio; Mendonça,Fernando F.; Lima,Arthur C.; Machado,Leonardo F.; Tosta,Vander C.; Farro,Ana Paula C.; Hostim-Silva,Maurício.

ABSTRACT The Atlantic goliath grouper, Epinephelus itajara , is a critically endangered species, threatened by illegal fishing and the destruction of its habitats. A number of other closely related grouper species found in the western Atlantic are also fished intensively. While some countries apply rigorous legislation, illegal harvesting followed by the falsification of fish products, which impedes the correct identification of the species, is a common practice, allowing the catch to be marketed as a different grouper species. In this case, molecular techniques represent an important tool for the monitoring and regulation of fishery practices, and are essential for the forensic identification of a number of different species. In the present study,...

REDESCRIPCIÓN DE POTAMOLITHUS SUPERSULCATUS PILSBRY, 1896 (GASTROPODA, TATEIDAE) DEL SUR DE LA CUENCA DEL PLATA

Palavras-chave: Conservation genetics; COI; Endangered species; Fishery management; Forensic genetics.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000300203

Pap. Avulsos de Zool. (São Paulo)

LUCÍA,MICAELA DE; GREGORIC,DIEGO EDUARDO GUTIÉRREZ.

RESUMEN El género Potamolithus Pilsbry, 1896 (Gastropoda; Tateidae), posee 31 especies, 22 de las cuales se hayan en la Argentina distribuidas principalmente en la cuenca Del Plata, definiendo al río Uruguay y al Río de la Plata como una zona caliente de diversidad en gasterópodos dulceacuícolas. Sin embargo, la mayoría de sus especies han sido descriptas solo por caracteres conquilógicos y, unas pocas tienen datos anatómicos, conllevando a la descripción de subespecies o morfos que se superponen unos a otros. A Potamolithus lapidum algunos autores le atribuyen cuatro subespecies (con datos conquiológicos, y una con datos parciales de anatomía blanda), sin embargo otros incluyen ocho “morfos”. Aquí nosotros damos un comienzo en el estudio de Potamolithus...

Heterobathmia pseuderiocrania Kristensen & Nielsen (Lepidoptera, Heterobathmiidae): identificación basada en DNA-barcoding y notas morfológicas e historia de vida de los estados inmaduros

Palavras-chave: Anatomía; COI; Conquilogía; Potamolithus lapidum; Especies vulnerables.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492017001700207

Rev. Bras. entomol.

Ramos,Rosa A.; Parra,Luis E..

Heterobathmia pseuderiocrania Kristensen & Nielsen (Lepidoptera, Heterobathmiidae): identification based on DNA-barcoding and notes on the morphology and life history of the immature stages. The larva morphology of the species Heterobathmia pseuderiocrania (Lepidoptera, Heterobathmiidae), a Nothofagus obliqua leafminer in Chile, is described. The tissue-feeding first and last instars are described. Also, the number of larval stages, some aspects of the biology and life cycle of the species are provided.

Male and female association in Trichomyia Haliday in Curtis, 1839 using a molecular approach (Diptera, Psychodidae, Trichomyiinae), and description of new species from Brazil

Palavras-chave: Biology; COI; Egg; Insecta; Morphology.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000100005

Rev. Bras. entomol.

Araújo,Maíra Xavier; Aragão,Marcos; Cordeiro,Danilo; Bravo,Freddy; Carvalho,Claudio José Barros de; Andena,Sergio R..

ABSTRACT A new species of Trichomyia from the state of Bahia, Brazil, is described and illustrated, and male and female are associated using DNA barcoding. Additionally, fragments of the COI of two other species, Trichomyia cerdosa Araújo & Bravo, 2016 and Trichomyia ituberensis Araújo & Bravo, 2016, and the females of two unidentified species, are sequenced.