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Characterisation of AC1: a naturally decaffeinated coffee Bragantia
Benatti,Luciana Benjamim; Silvarolla,Maria Bernadete; Mazzafera,Paulo.
We compared the biochemical characteristics of the beans of a naturally decaffeinated Arabica coffee (AC1) discovered in 2004 with those of the widely grown Brazilian Arabica cultivar "Mundo Novo" (MN). Although we observed differences during fruit development, the contents of amino acids, organic acids, chlorogenic acids, soluble sugars and trigonelline were similar in the ripe fruits of AC1 and MN. AC1 beans accumulated theobromine, and caffeine was almost entirely absent. Tests on the supply of [2-14C] adenine and enzymatic analysis of theobromine synthase and caffeine synthase in the endosperm of AC1 confirmed that, as in the leaves, caffeine synthesis is blocked during the methylation of theobromine to caffeine. The quality of the final coffee...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; Caffeine; Decaffeination; Beverage quality.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000200001
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Mineral nutrition and caffeine content in coffee leaves Bragantia
MAZZAFERA,PAULO.
The effect of nutrient supply on the caffeine content of coffee (Coffea arabica L.) leaves was investigated. Seeds were germinated in nutrient-agar media lacking N, P, K, Ca, Mg, S, Zn, B or Mo. The control treatment contained all essential nutrients. The caffeine concentration was determined seven months after seed sowing when the seedlings have 3 to 4 pair of leaves. The omission of K induced the highest caffeine content in the leaves (24.5 g.kg-1). Caffeine in the control treatment was 21.9 g.kg -1. Absence of P induced the lowest content, 17.5 g.kg-1.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Caffeine; Coffea arabica L.; Mineral nutrition.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051999000200018
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Hydroxypropylcellulose controlled release tablet matrix prepared by wet granulation: effect of powder properties and polymer composition BABT
Teixeira,Antonio Zenon Antunes.
The aim of this study was to attain 100% drug release of caffeine after 24 h from hydroxypropylcellulose (HPC) tablet matrices and to investigate the effect of co-excipient. Physical properties of the powders were evaluated and suggested for a wet granulation process. The tablet containing caffeine was formulated by different weight ratios of hydrophilic polymers. The results of polymer evaluation confirmed that the increase of HPC level with the same drug content significantly decreased the rate of drug release. The presence of co-polymer excipients carboxymethylcellulose (CMC) and polyvinylpyrrolidone (PVP) in the tablet matrix was also investigated. The release rate was also controlled by low levels of CMC (<10%) while PVP did not show any...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Controlled release; Caffeine; Hydroxypropylcellulose (HPC); Polyvinylpyrrolidone (PVP); Carboxymethylcellulose (CMC); Lactose.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100021
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Sobre a Padronização de um Método Prático Para a Dosagem da Cafeína BABT
Bührer,Nilton E..
In this work the author puts in evidence a practical and at the same time rapid method for the determination of the cafein quantity in erva-mate, that may be utilized to perfection in food and industrial analyses or others that not demand scientific rigour. The author presents a description of the method and makes a comparison with the classical method of Grandval-Lajoux, showing that the differences between the one and the other are practically nil. In conclusion, the result of the three analyses of the same sample of erva-mate, made by the Grandval-Lajoux method are cited, show that the same differences appear as those shown between the rapid method suggested by the author and the classical method. In this case, the maximum difference was 0,06% and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caffeine; Erva-mate; Caffeine assay.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500007
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The influence of caffeine and thymol on the survival, growth and reproduction of Subulina octona (Brugüière, 1789) (Mollusca, Subulinidae) BABT
Ferreira,Paula; Soares,Geraldo Luiz Gonçalves; D'ávila,Sthefane; Bessa,Elisabeth Cristina de Almeida.
Subulina octona is a terrestrial snail which serves as an intermediate host for the parasites. It is also an agricultural pest. The aim of this work was to assess, during 120 days, the effects of caffeine and thymol at 2.5 g/L and 5 g/L on the hatchability, survival after hatching, growth and reproduction of S. octona under the laboratory conditions. A total of 240 eggs, 240 juveniles aged 10-day-old, and 240 aged 30-day-old were tested. The results showed that thymol (at 2.5 g/L and 5 g/L) and caffeine (at 5 g/L) acted as ovicides. In the 10-day-old juveniles, caffeine at 5 g/L caused 25% mortality and at 2.5 g/L it caused 30% mortality. Thymol at 2.5 and 5 g/L caused 20 and 22.5% mortality, respectively. In the 30-day-old juveniles, caffeine at 5 g/L...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caffeine; Thymol; Molluscicide; Subulina octona; Snail-terrestrial.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400018
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Caffeine formation by suspension cultures of Coffea dewevrei BABT
Sartor,Rosana Mary; Mazzafera,Paulo.
The low caffeine content in leaves of C. dewevrei (~ 0.5 mg/g) is due to a low biosynthesis associated with a fast degradation. On the other hand, high biosynthesis and low degradation confer a higher content (~ 8 mg/g) in leaves of C. arabica. In this work it was observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with labelled carbon dioxide showed a significant accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves, and therefore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Caffeine; Cell suspension culture; Methylxanthines.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100009
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Stability of phenolic compounds and antioxidant capacity of regular and decaffeinated coffees BABT
Vicente,Silvio José Valadão; Queiroz,Yara Severino; Gotlieb,Sabina Léa Davidson; Torres,Elizabeth Aparecida Ferraz da Silva.
This study compared the regular and decaffeinated coffees in relation to antioxidant capacity, levels of some antioxidant molecules and stability of these parameters over a six-month period under different storage conditions. The regular coffee samples analyzed right after the industrial production showed higher antioxidant capacity (ORAC and DPPH), the same levels of phenolic compounds and higher levels of phenolic acids than decaffeinated coffee. After six months, the closed packs of both the grades kept under vacuum at 20°C did not show significant changes from the initial results; the open packs stored at 4°C showed small but statistically significant reductions and the open packs stored at 20°C showed higher and statistically significant losses (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Antioxidant capacity; Phenolic compounds; Caffeine; ORAC; DPPH.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100016
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Spectrophotometric determination of tannins and caffeine in preparations from Paullinia cupana var. sorbilis BABT
Pelozo,Maria Inez de Godoy; Cardoso,Mara Lane Carvalho; Mello,João Carlos Palazzo de.
The present work was designed to quantify the caffeine and total polyphenols in the extractive solution and the granulated form from the seeds of Paullinia cupana var. sorbilis, by the spectrophotometric method. The method showed linearity for the caffeine and polyphenols in the range of 5-25 µg/ml and 2.4-5.6 µg/ml respectively. The solutions of the semipurified fraction (EPA) and granulated form (GRA) showed linear responses in the range of 0.288-0.672 and 0.4-1.2 µg/ml, respectively. The precision and accuracy were determined for the EPA solution at a concentration of 100 µg/ml. The spectrophotometric method performed well in quantifying the caffeine and total polyphenols.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caffeine; Paullinia cupana; Total polyphenols; Spectrophotometric quantification assay.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300002
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Influence of integral and decaffeinated coffee brews on metabolic parameters of rats fed with hiperlipidemic diets BABT
Gomes,Júlia Ariana de Souza; Faria,Bruno Generoso; Silva,Viviam de Oliveira; Zangerônimo,Márcio Gilberto; Miranda,José Rafael; Lima,Adriene Ribeiro de; Pereira,Rosemary Gualberto Fonseca Alvarenga; Pereira,Luciano José.
The objective of this study was to evaluate the influence of integral and decaffeinated coffee brews (Coffea arabica L and C. canephora Pierre) on the metabolic parameters of rats fed with hyperlipidemic diet. Thirty male Wistar rats (initial weight of 270 g ± 20 g) were used in the study, which were divided into six groups five each. The treatments were normal diet, hyperlipidemic diet, hyperlipidemic diet associated with integral coffee arabica or canephora brews (7.2 mL/kg/day) and hyperlipidemic diet associated to decaffeinated arabica, or canephora brews, using the same dosage. After 41 days, performance analyses were conducted.The rats were then euthanized and the carcasses were used for the analysis of dried ether extract and crude protein....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipids; Adipocytes; Hyperlipidemia; Caffeine; Decaffeination.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500015
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Coffee has hepatoprotective benefits in Brazilian patients with chronic hepatitis C even in lower daily consumption than in American and European populations BJID
Machado,Silmara Rodrigues; Parise,Edison Roberto; Carvalho,Luciana de.
The potential role of coffee as a hepatoprotective substance for chronic liver diseases has been widely discussed. Our main aim was to evaluate the effect of coffee intake regarding clinical, biochemical tests and liver biopsy data in treatment naïve patients with chronic hepatitis C. One hundred and thirty-six patients with chronic hepatitis C, diagnosed through liver biopsy, or by means of clinical, ultrasound or endoscopic signs of cirrhosis, were assessed by determination of biochemical tests, metabolic and morphological alterations. Food frequency was scrutinized by using a structured questionnaire. Coffee intake represented more than 90% of the total daily caffeine, and the 75th percentile was 4-Brazilian coffee-cup/day (>255mL/day or >123mg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Caffeine; Hepatitis C; Fibrosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200170
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Bovine serum albumin potentiates caffeine- or ATP-induced tension in human skinned skeletal muscle fibers BJMBR
Human skinned muscle fibers were used to investigate the effects of bovine serum albumin (BSA) on the tension/pCa relationship and on the functional properties of the Ca2+-release channel of the sarcoplasmic reticulum (SR). In both fast- and slow-type fibers, identified by their tension response to pSr 5.0, BSA (0.7-15 µM) had no effect on the Ca2+ affinity of the contractile proteins and elicited no tension per se in Ca2+-loaded fibers. In contrast, BSA (&gt;1.0 µM) potentiated the caffeine-induced tension in Ca2+-loaded fibers, this effect being more intense in slow-type fibers. Thus, BSA reduced the threshold caffeine concentration required for eliciting detectable tension, and increased the amplitude, the rate of rise and the area under the curve...
Palavras-chave: Calcium-release channel; Sarcoplasmic reticulum; Human muscle fiber; Bovine serum albumin; Caffeine.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500017
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Effects of caffeine on learning and memory in rats tested in the Morris water maze BJMBR
Angelucci,M.E.M.; Cesário,C.; Hiroi,R.H.; Rosalen,P.L.; Cunha,C. Da.
We studied some of the characteristics of the improving effect of the non-specific adenosine receptor antagonist, caffeine, using an animal model of learning and memory. Groups of 12 adult male Wistar rats receiving caffeine (0.3-30 mg/kg, ip, in 0.1 ml/100 g body weight) administered 30 min before training, immediately after training, or 30 min before the test session were tested in the spatial version of the Morris water maze task. Post-training administration of caffeine improved memory retention at the doses of 0.3-10 mg/kg (the rats swam up to 600 cm less to find the platform in the test session, P<=0.05) but not at the dose of 30 mg/kg. Pre-test caffeine administration also caused a small increase in memory retrieval (the escape path of the rats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Memory; Learning; Caffeine; Methylxanthines; Adenosine receptor antagonist; Morris water maze.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000013
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Effects of caffeine on time to exhaustion in exercise performed below and above the anaerobic threshold BJMBR
Denadai,B.S.; Denadai,M.L.D.R..
Controversy still exists concerning the potential ergogenic benefit of caffeine (CAF) for exercise performance. The purpose of this study was to compare the effects of CAF ingestion on endurance performance during exercise on a bicycle ergometer at two different intensities, i.e., approximately 10% below and 10% above the anaerobic threshold (AT). Eight untrained males, non-regular consumers of CAF, participated in this study. AT, defined as the intensity (watts) corresponding to a lactate concentration of 4 mM, was determined during an incremental exercise test from rest to exhaustion on an electrically braked cycle ergometer. On the basis of these measurements, the subjects were asked to cycle until exhaustion at two different intensities, i.e.,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Caffeine; Anaerobic threshold; Endurance; Lactate; Perceived exertion.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400017
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Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA BJMBR
Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A..
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019
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Caffeine intake by patients with autosomal dominant polycystic kidney disease BJMBR
Vendramini,L.C.; Nishiura,J.L.; Baxmann,A.C.; Heilberg,I.P..
Because caffeine may induce cyst and kidney enlargement in autosomal dominant polycystic kidney disease (ADPKD), we evaluated caffeine intake and renal volume using renal ultrasound in ADPKD patients. Caffeine intake was estimated by the average of 24-h dietary recalls obtained on 3 nonconsecutive days in 102 ADPKD patients (68 females, 34 males; 39 ± 12 years) and compared to that of 102 healthy volunteers (74 females, 28 males; 38 ± 14 years). The awareness of the need for caffeine restriction was assessed. Clinical and laboratory data were obtained from the medical records of the patients. Mean caffeine intake was significantly lower in ADPKD patients versus controls (86 vs 134 mg/day), and 63% of the ADPKD patients had been previously aware of caffeine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polycystic kidneys; ADPKD; Cyclic AMP; Caffeine; Nutrition; Renal volume.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900007
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Inhibition of the sarcoplasmic reticulum Ca2+ pump with thapsigargin to estimate the contribution of Na+-Ca2+ exchange to ventricular myocyte relaxation BJMBR
Bassani,R.A.; Bassani,J.W.M..
Relaxation in the mammalian ventricle is initiated by Ca2+ removal from the cytosol, which is performed by three main transport systems: sarcoplasmic reticulum Ca2+-ATPase (SR-A), Na+-Ca2+ exchanger (NCX) and the so-called slow mechanisms (sarcolemmal Ca2+-ATPase and mitochondrial Ca2+ uptake). To estimate the relative contribution of each system to twitch relaxation, SR Ca2+ accumulation must be selectively inhibited, usually by the application of high caffeine concentrations. However, caffeine has been reported to often cause changes in membrane potential due to NCX-generated inward current, which compromises the reliability of its use. In the present study, we estimated integrated Ca2+ fluxes carried by SR-A, NCX and slow mechanisms during twitch...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Myocardium; Relaxation; Calcium fluxes; Sodium-calcium exchanger; Sarcoplasmic reticulum calcium ATPase; Caffeine.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200014
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Effects of caffeine on the electrophysiological, cognitive and motor responses of the central nervous system BJMBR
Deslandes,A.C.; Veiga,H.; Cagy,M.; Piedade,R.; Pompeu,F.; Ribeiro,P..
Caffeine is the most consumed psychoactive substance in the world. The effects of caffeine have been studied using cognitive and motor measures, quantitative electroencephalography (qEEG) and event-related potentials. However, these methods are not usually employed in combination, a fact that impairs the interpretation of the results. The objective of the present study was to analyze changes in electrophysiological, cognitive and motor variables with the ingestion of caffeine, and to relate central to peripheral responses. For this purpose we recorded event-related potentials and eyes-closed, resting EEG, applied the Stroop test, and measured reaction time. Fifteen volunteers took caffeine (400 mg) or placebo in a randomized, crossover, double-blind...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative electroencephalography; P300; Caffeine; Cognitive and motor measures; Psychostimulants.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700011
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Effect of caffeine and tannins on cultivation and fructification of Pleurotus on coffee husks BJM
Fan,Leifa; Soccol,Andréa Thomaz; Pandey,Ashok; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
The objective of this work was to study the effect of caffeine and tannins on Pleurotus sp. cultivation and to evaluate the feasibility of using coffee husks as substrate for mushroom cultivation. Eight strains of P. ostreatus and two strains of P. sajor-caju were screened on a medium prepared from agar extract of coffee husk. Based on best mycelial growth and biomass production, the strain P. ostreatus LPB 09 was selected for detailed studies. With the increase of caffeine concentration, the mycelial growth and the biomass production decreased, and no growth was observed when concentration of caffeine was 2500 mg/L. Furthermore, Pleurotus did not degrade the caffeine, but absorbed it. Tannin under 100 mg/L in the medium stimulated the growth of mycelia,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cultivation; Pleurotus; Caffeine; Tannin; Detoxification.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400003
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Caffeine degradation by Rhizopus delemar in packed bed column bioreactor using coffee husk as substrate BJM
Tagliari,Cristiane Vanessa; Sanson,Raquel K.; Zanette,André; Franco,Telma Teixeira; Soccol,Carlos Ricardo.
Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10(6) spores/g substrate, aeration rate 60 mL/min...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decaffeination; Fermentation; Caffeine; Theophylline; Bioreactor; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500035
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Neuroprotective and MAOB inhibitory effects of a series of caffeine-8-thioglycolic acid amides BJPS
Kasabova-Angelova,Alexandra; Kondeva-Burdina,Magdalena; Mitkov,Javor; Georgieva,Maya; Tzankova,Virginia; Zlatkov,Alexander.
The effects of new derivatives of caffeine-8-thioglycolic acid (100 µM) on isolated rat brain synaptosomes, human neuroblastoma cell line SH-SY5Y and human recombinant MAOB enzyme (hMAOB) (1 µM) were evaluated. Most of the compounds, administered alone, didn’t show statistically significant neurotoxic effects on SH-SY5Y, when compared to the control (non-treated cells). Of all studied structures JTA-2Ox, JTA-11, JTA-12 and JTA-13 decreased cell viability. In combination with 6-hydroxydopamine (6-OHDA) (100 µM), only JTA-1 and JTA-2 revealed neuroprotective effects, stronger than those of caffeine. All compounds administered alone revealed, neurotoxic effects on synaptosomes, as compared to non-treated synaptosomes. JTA-1, JTA-2 and JTA-3 showed lowest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neuroprotection; Caffeine; Synaptosomes; SH-SY5Y; HMAOB.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100529
Registros recuperados: 43
Primeira ... 123 ... Última
 

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