|
|
|
| Registros recuperados: 101 | |
|
| |
|
|
| Lin,Ling; Kan,Xianzhao; Yan,Hao; Wang,Danni. |
| The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from... |
| Tipo: Journal article |
Palavras-chave: Bacillus thuringiensis; Cellulase; Cellulose-degrading; Characterization. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002 |
| |
|
| |
|
| |
|
|
| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
| |
|
| |
|
|
| Purquerio,Luis Felipe V; Lago,Antonio A do; Passos,Francisco Antonio. |
| The occurrence of hardseedness and the low percentage of seed germination are major challenges when growing okra. For this reason, five elite lines of okra developed at the Campinas Agronomic Institute (IAC), three with angular pods (lines 8.1, 20.1.2 and 47.1) and two with cylindrical pods (lines 13.1.2 and 20.1.R), were evaluated for seed quality and compared to two commercial seed samples (Horticeres Sementes and Sakata Seed Sudamerica) of cultivar Santa Cruz 47. Seeds were field produced in an experiment set in the Technological Park of the Horticulture Center of IAC, in Campinas, São Paulo State, Brazil, in a randomized block design, with three replications and 20-plant plots. Fruits were harvested 25, 35, 45, 55, 65 and 75 days after anthesis (DAA).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abelmoschus esculentus Moench; Advanced generation lines; Characterization; Seed quality. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362010000200017 |
| |
|
|
| Nascimento,Naysa Flávia F do; Rêgo,Elizanilda R do; Nascimento,Mayana F; Bruckner,Cláudio H; Finger,Fernando L; Rêgo,Mailson M do. |
| ABSTRACT The cultivation of potted peppers as ornamental plants has increased considerably throughout the planet. In Brazil, this crop is more recent and still lacks cultivars. In order to increase the available variability and obtain new cultivars, the interspecific hybridization is very useful in add a desirable attribute that occurs in one species to another species, resulting in a new cultivar of agronomic interest. The objective of this study was to characterize parents and interspecific hybrids based on 27 quantitative traits and evaluate their genetic diversity by multivariate procedures. Parents of seven pepper genotypes were crossed, and, seven hybrids were generated. The experiment was conducted in a completely randomized design. Data were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum spp.; Hybridization; Relative importance; Breeding; Characterization. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300315 |
| |
|
|
| Santiago-Luna,Eric G; Carrillo-Rodríguez,José C; Chávez-Servia,José L; Perales-Segovia,Catarino; Heredia-Garcia,Elena. |
| ABSTRACT In a collection of costeño peppers conserved by indigenous producers from the municipalities of Santa Maria Tonameca and Santo Domingo de Morelos, Oaxaca, Mexico, a description and classification of agromorphological variation was undertaken by characterization of 46 populations in a greenhouse distributed under complete randomized block design with three replicates. Days to first flowering, plant and fruit traits and yield per plant were evaluated. Significant differences were detected for all traits except for plant height at 120 days after planting. In a principal component analysis, the variables of green and dry weight of 15 fruits, average fruit weight, number of fruits and yield per plant were the characteristics with major descriptive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum annuum; Phenotypic diversity; Plant genetic resources; Characterization; Multivariate analysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300399 |
| |
|
| |
|
| |
|
|
| FACCIOLI-MARTINS, P. Y.; SOUZA, V. de; PINHEIRO, R. R.. |
| Resumo: Esta publicação tem por objetivo descrever a história, a composição da Coleção de trabalho de Microrganismos Patogênicos a Caprinos e Ovinos, e de que forma ela tem conservado, caracterizado, documentado e utilizado seus isolados. A coleção foi criada em 2012 e atualmente é composta por 141 cepas bacterianas e seis cepas virais, pertencentes às espécies Corynebacterium pseudotuberculosis, Staphylococcus aureus e ao gênero Lentivirus. Esses isolados são utilizados para produção de antígenos para imunodiagnósticos, em diagnósticos moleculares e na avaliação de resistência a antimicrobianos e ação de anti-sépticos e antimicrobianos. Participa de projetos das coleções institucionais e de gestão da qualidade. [Collection of Goat and Sheep Pathogenic... |
| Tipo: Artigo de divulgação na mídia (INFOTECA-E) |
Palavras-chave: Caracterização; Germplasm bank; Characterization; Diagnosis; Quality; Pathogenic Microorganisms; Caprino; Ovino; Banco de Germoplasma; Conservação; Resistência Genética; Diagnostico; Qualidade; Sheep diseases; Goat diseases; Germplasm conservation; Conservation banks; Genetic resistance; Small ruminants; Brazil. |
| Ano: 2020 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1123793 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia. |
| Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared... |
| Tipo: Text |
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf |
| |
|
|
| Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine. |
| Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf |
| |
|
|
| Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques. |
| This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively.... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf |
| |
|
|
| Dufourcq, R.; Chalkiadakis, Eletherios; Fauchon, M.; Deslandes, Eric; Kerjean, V.; Chanteau, S.; Petit, Elodie; Guezennec, Jean; Dupont-rouzeyrol, M.. |
| Marine bacteria are a rich source of bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of New Caledonia. We obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). Ten out of the most promising strains were cultured, fractionated and screened for antibacterial activity. Four of them (NC282, NC412, NC272 and NC120) showed at least an activity against reference and... |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Characterization; Marine bacteria; New Caledonia; Screening. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00174/28552/26994.pdf |
| |
| Registros recuperados: 101 | |
|
|
|
