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| Registros recuperados: 25 | |
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| Hao,Zhikui; Cai,Yujie; Liao,Xiangru; Zhang,Xiaoli; Fang,Zhiyou; Zhang,Dabing. |
| The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Medium optimization; Response surface method; Inulin; Chitinolyticbacter meiyuanensis SYBC-H1. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019 |
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| Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali. |
| Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022 |
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| Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V.. |
| In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002 |
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| Registros recuperados: 25 | |
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