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Registros recuperados: 67 | |
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OKAWARA, Sumika; HAMANO, Seizo; TETSUKA, Masafumi; 手塚, 雅文. |
Glycerol plays multi-functional roles in cellular physiology. Other than forming the backbone molecule for glycerophospholipid and triglyceride (TG), glycerol acts as an energy substrate for glycolysis. Spermatozoa are known to utilize glycerol for energy production, but there are no reports of this in oocytes. In this study, the value of glycerol as an energy substrate for bovine oocyte maturation (Exp. 1) and the gene expression of glycerol kinase (GK), an enzyme crucial for cellular glycerol utilization, in bovine oocytes and early embryos (Exp. 2) were examined. In Exp. 1, in vitro maturation (IVM) was conducted using synthetic oviduct fluid supplemented with/without glucose (1.5 mM) and/or glycerol (1.0 mM), and maturation rate, degree of cumulus... |
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Palavras-chave: Bovine; Embryo; Glycerol; Glycerol kinase (GK); Oocyte maturation. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2829 |
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ABE, Yasuyuki; SUWA, Yoshinori; YANAGIMOTO-UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志. |
Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further... |
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Palavras-chave: Dog; Embryo; Preimplantation development. |
Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3029 |
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Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio. |
Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced... |
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Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus. |
Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532 |
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Fowler, João Antonio Pereira; Sturion, José Alfredo; Zuffellato-Ribas, Katia Christina. |
Ilex paraguariensis St.Hil., popularly named erva-mate in Brazil, is an indigenous species of the Araucaria Forest with a large geographic distribution and a high social and economic importance. The seeds of this species exhibits physiologic and seed coat impermeability dormancies and needs stratification on sand in order to break them. This work aimed to evaluate the influence of genotype -environment interaction on embryo development of erva-mate seeds formed under different environmental conditions. The fruits were collected in Cascavel, Toledo and Campo Mourão, State of Paraná, and in Soledade, State of Rio Grande do Sul, in February 2003. The experiment was carried out on Forest Seed Laboratory of Embrapa Florestas, in a completely randomized design... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ilex paraguariensis; Embryo; Germination; Dormancy. Embrião; Germinação; Dormência; Erva-mate.. |
Ano: 2010 |
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/134 |
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Oyama,Sayuri de Oliveira; Souza,Luiz Antonio de. |
(Morphology and anatomy of the developing fruit of Maclura tinctoria, Moraceae). Maclura tinctoria (L.) D. Don ex Steudel was selected for the present study due of its economic and medicinal importance. The purpose of this investigation is to present a detailed description of the fruit development, specially by: (a) defining the fruit type presented by the species, and (b) characterizing the seed type of the species based upon the presence or not of mechanical tissue on the seed-coat. The fruit originates from the subglobose female inflorescence which consists of small unipistillate flowers with superior ovary, unilocular and uniovular apical placentation. The mature fruit is multiple, constituted of small drupes. The ovule is ana-campylotropous,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drupe; Embryo; Integument; Pericarp; Perigone. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000200006 |
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Sivasubramaniam,K.; Selvarani,K.. |
Jamun (Syzygium cumini L. Skeels) (Black plum, Damson plum) fruits weigh between 2-5 g at maturity. Fresh seeds represented 20-80% of the total fruit weight; the seed coat and cotyledons contributed 6% and 94% to the total seed weight respectively, while the weight of the embryonic axis was insignificant. Only the embryonic axis stained with Tetrazolium, not the cotyledons. The seeds are polyembryonic with up to four embryos, of which at most three germinate. Decoated seeds germinated faster than coated seeds under nursery conditions, with high significant germination percentages, dry matter production rates and vigor indices. The lack of staining of the cotyledon by tetrazolium was probably due to the presence of an impermeable layer. Decoating seeds for... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Decoated seeds; Embryo; Myrtaceae; Polyembryony; Tetrazolium. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400012 |
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Gualhanone,A; Furlan,RL; Fernandez-Alarcon,MF; Macari,M. |
Two experiments were carried out to study the effect of breeder age on incubation parameters (hatchability, eggshell thickness, egg surface temperature and chick weight). In Exp. 1, fertile eggs (30- and 60-wk-old breeders) were incubated at three different temperatures (36.8, 37.8 and 38.8 ºC). Eggshell surface temperature was measured by attaching a thermocouple to the shell and data were collected in a datalogger every ten minutes. This study was conducted according to a 3 x 2 factorial design (three temperatures and two breeder ages). Data revealed that eggshell surface temperature changed according to incubation temperature, with the main increase occurring between 10 and 13 days of incubation, and that the maximum increase in eggshell surface... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeder; Development; Embryo; Temperature; Incubation. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000100002 |
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Roriz,BC; Sgavioli,S; Garcia,RG; Nääs,IA; Domingues,CHF; Caldara,FR; Rombola,LG; Ayla,CM; Bernnecke,K. |
ABSTRACT Long storage periods may increase embryo mortality. The objective of this study was to evaluate the effects of storage period on the weight loss, eggshell surface temperature, hatchability, and embryonic mortality of Japanese quail eggs. Two hundred fertile eggs were collected from a flock of 30-week-old Japanese quails (Coturnix coturnix japonica). The eggs were collected for 10 consecutive days after lay, and immediately incubated. A completely randomized experimental design with 10 treatments, corresponding to the number of days eggs were stored between egg collection and setting, with 20 replicates each, was applied. Egg weight loss increased with storage period duration, starting on day 6 (2.1%, on average) and reached 3.26%, on average, in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Hatchability; Incubation; Laying; Temperature. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400589 |
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Maiorka,A; Silva,AVF; Santin,E; Bruno,LDG; Boleli,IC; Macari,M. |
ABSTRACT This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeder age; Embryo; Microvillus; Small intestine; Villus. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600079 |
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Onbaşılar,EE; Güngör,ÖF; Demir,T; Erbay,FK Elibol; Kahraman,M; Taban,S; Çalık,A; Pirpanahi,M. |
ABSTRACT Layer hybrids are more superior in the egg yield than pure breeds. But this superiority causes some problems in the bones during the production period. There are a lot of differences among genotypes in the production period that have been examined extensively, however, the differences throughout incubation period aren’t known yet. Thus, the objective of this study was to evaluate the differences in femur properties of pure breed and layer hybrid embryos during the incubation period. A total of 354 fresh hatching eggs were used from one hybrid (Lohman White) and two pure breeds (Denizli & Gerze). The eggs were incubated. Hatching eggs from each genotype at the beginning of embryonic days (E) 19 and E21 were examined. At these embryonic ages, 12... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Layer; Pure breed; Hybrid; Embryo; Femur. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400805 |
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Araújo,ICS; Leandro,NSM; Mesquita,MA; Café,MB; Mello,HHC; Gonzales,E. |
ABSTRACT Two experiments were conducted to evaluate the effect of broiler breeder age and incubator type on hatching parameters, hatch window, embryo diagnosis results, and hatchling physical quality. The treatments consisted of a combination of three broiler breeder ages (29, 35 and 59 weeks of age) and two incubator types (single stage, SS; or and multiple stage, MS). A completely randomized design in a 3x2 factorial arrangement was applied. In Experiment I, 1,896 eggs were used and 360 eggs in Experiment II. There was an interaction between breeder age and incubator type only for hatchling physical quality score. Independently of incubator type, hatchability rate, late embryo mortality, and egg contamination were higher in the eggs laid by older... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Hatch window; Multiple-stage and single-stage incubation. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600017 |
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Onbaşilar,EE; Erdoğan,M; Erol,S; Kahraman,M; Güngör,ÖF. |
ABSTRACT Pure breeds are important due to the fact that they adapt well to poor husbandry conditions. However, gene expression of digestive enzymes and nutrient transporters of pure breeds and layer hybrids have not been studied yet. Therefore, this study was directed to detect the gene expression of digestive enzymes and nutrient transporters in the yolk sac membrane and small intestine of the embryos in layer hybrid and pure breeds. A total of 354 hatching eggs were collected from 29 wk old Denizli, Gerze and Lohman White (LW) breeder flocks. Twelve eggs from each genotype were selected at embryonic day 19 (E19) and day 21 (E21). APN, SI, PepT1, EAAT3, CAT1, B0AT, SGLT1 and GLUT5 mRNA expressions were evaluated in the yolk sac membrane (YSM) and small... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Gene expression; Layer hybrid; Pure breed. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300435 |
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Cordeiro,Maria Cristina Rocha; Pinto,Alberto Carlos Queiróz; Ramos,Víctor Hugo Vargas; Faleiro,Fábio Gelape; Fraga,Lilia Marta Santos. |
Mango can be propagated by seeds or by grafting. For commercial purpose, grafting is the most appropriate method because it maintains the genetic characters from the propagated variety. To obtain grafted mango it is important to use polyembryonic varieties as rootstock since they produce a zygotic and many nucellar plantlets. The nucellar plantlets maintain the genetics of the mother-plant thus, are preferred for grafting since they supposedly give more uniformity to the orchard. In general, nurserymen use the most vigorous plantelet to graft, believing that they are nucellar. But, orchard disuniformities on height and yield are very common among mango trees of commercial orchards in Northeast region. The objective of this paper was to identify the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plantlet identification; Embryo; Rootstock; Orchard uniformity. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300025 |
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IOSSI,EMERSON; MORO,FABIOLA VITTI; VIEIRA,BRUNO GUILHERME TORRES LICURSI; BARBOSA,RAFAEL MARANI; VIEIRA,ROBERVAL DAITON. |
ABSTRACT Queen palm is a tropical species of ecological importance and economic potential in various uses, including food. However, the knowledge about the nutritional value of fruits and the estimation of seed viability are still scarce. The aims of this work were to study the biometrics and chemical composition of fruits and seeds; describe the anatomy of embryos and assess the viability of the tetrazolium test. Mature fruits were collected, processed and submitted to biometry, water content, pulp and seeds chemical composition, germination and emergence in the laboratory evaluations. For the tetrazolium test, whole embryos were immersed in solution at 40 °C for one, two, four and six hours and compared with germination. The fruit pulp is rich in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Queen palm; Embryo; Germination; Palm; Pyrene. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000402001 |
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Silva,Vanessa Neumann; Cicero,Silvio Moure; Bennett,Mark. |
Structural differences such as abnormalities, damage and free spaces in seeds may affect germination. The aim of this study was to study the relationship between eggplant seed morphology and seed germination. Ten seed lots of the eggplant cultivar Embu were evaluated by X-ray image analysis and the germination test. Seed image analysis was performed by Image Pro Plus® software and the whole seed area and free space between the embryo and endosperm were measured. The internal seed area filled by the embryo and endosperm was calculated from the difference between the whole seed and free space areas. Based on these results and visual seed analysis, seeds were classified into three categories and information on germination was obtained for each one. X-ray... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: X-ray; Solanum melongena L.; Embryo. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000400010 |
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Amorim,Lincoln da Silva; Torres,Ciro Alexandre Alves; Siqueira,Luiz Gustavo Bruno; Fonseca,Jeferson Ferreira da; Guimarães,José Domingos; Carvalho,Giovanni Ribeiro de; Alves,Nadja Gomes; Oliveira,Margarida Maria Nascimento Figueiredo de. |
The effects of feeding urea on embryo production, quality and developmental stages and follicular status at the beginning of a superovulatory treatment of Toggenburg does fed urea diet were investigated in this study. Eighteen females were randomly allocated to receive diets with: no urea (control, n=8), and 2.4% urea dietary dry matter (n=10) UDM. The embryo recovery was performed at day 7 or 8 of estrus cycle by transcervical technique and classified according to quality and developmental stage. The follicular status was determined by transrectal ultrasonography at the first day of FSH injection. The number and quality of embryos were not affected by dietary urea concentration. Embryos from does treated with 2.4% UDM were recovered at advanced stage.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Follicle; Goat; Superovulation; Urea. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000200007 |
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Registros recuperados: 67 | |
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