|
|
|
| Registros recuperados: 56 | |
|
|
| Freitas,Suzane Fonseca; Moreira,Heden Luiz Marques; Souza,Daiane Machado; Moreira,Carla Giovane Ávila; Tavares,Rafael Aldrighi; Dionello,Nelson José Laurino. |
| ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Genotyping; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409 |
| |
|
|
| Ganopoulos,Ioannis; Tourvas,Nikolaos; Xanthopoulou,Aliki; Aravanopoulos,Filippos A.; Avramidou,Euaggelia; Zambounis,Antonios; Tsaftaris,Athanasios; Madesis,Panagiotis; Sotiropoulos,Thomas; Koutinas,Nikolaos. |
| ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Genotyping; Microsatellite markers; Multivariate analysis; Phenotyping. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600509 |
| |
|
|
| Ribeiro,Carlos Alexandre Gomes; Pinto,Marcos de Oliveira; Maciel,Talles Eduardo Ferreira; Pastina,Maria Marta; Barros,Everaldo Gonçalves de; Guimarães,Claudia Teixeira. |
| ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Fingerprinting; Genotyping; Cultivar protection. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163 |
| |
|
|
| Machado,Elizabeth S.; Lambert,John S.; Afonso,Adriana O.; Cunha,Silvia M.; Oliveira,Ricardo H.; Tanuri,Amilcar; Sill,Anne M.; Soares,Marcelo A.. |
| Although mother-to-child HIV transmission prevention has slowed down pediatric HIV infection in developed countries, large numbers of infants still become infected in developing nations. Data on pediatric HIV infection is however largely scarce. In this study, we have overviewed clinical, laboratory and genotypic data from a large cohort of HIV-infected infants regularly followed at two pediatric HIV outpatient clinics in Rio de Janeiro, Brazil. Children on antiretroviral therapy, as well as drug-naïve, newly diagnosed infants were analyzed. Prevalence of drug resistance mutations, as well as immunological and virological responses to therapy were evaluated. Additionally, HIV-1 subtype frequencies and their distribution over the course of the epidemic were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Pediatric; Drug resistance; Clinical; Genotyping; Subtype. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400008 |
| |
|
|
| PAIVA,DANIELA IVANA; CASCALES,JIMENA; ROSETTI,MARÍA EVA NATALIA; SCHERER,RAFAEL ALEJANDRO; GAUCHAT,MARÍA ELENA; GOTTLIEB,ALEXANDRA MARINA. |
| Abstract We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chloroplastidic microsatellites; Genetic diversity; Genotyping; ISSR markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100717 |
| |
|
|
| SANTOS,PAULO C.C. DOS; LOPES,HELDER F.S.; ALCALDE,ROSANA; GONSALEZ,CLÁUDIO R.; ABE,JAIR M.; LOPEZ,LUIS F.. |
| ABSTRACT The high variability of HIV-1 as well as the lack of efficient repair mechanisms during the stages of viral replication, contribute to the rapid emergence of HIV-1 strains resistant to antiretroviral drugs. The selective pressure exerted by the drug leads to fixation of mutations capable of imparting varying degrees of resistance. The presence of these mutations is one of the most important factors in the failure of therapeutic response to medications. Thus, it is of critical to understand the resistance patterns and mechanisms associated with them, allowing the choice of an appropriate therapeutic scheme, which considers the frequency, and other characteristics of mutations. Utilizing Paraconsistents Artificial Neural Networks, seated in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial Neural Networks; HIV; Genotyping; Paraconsistent logic; Paraconsistents Artificial Neural Networks; Pattern recognition. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100323 |
| |
|
|
| Trevisani,N.; Barros,L.D.; Vieira-Neto,A.; Sartor,A.A.; Souza,A.P.; Garcia,J.L.; Moura,A.B.. |
| ABSTRACT Toxoplasmosis is a widespread zoonosis that can infect warm-blooded animals including birds and humans, and chickens are considered to be indicators of environmental contamination. In Brazil, Toxoplasma gondii has a non-clonal population structure composed of three lineages (I, II, and III), presenting high recombination, and resulting in wide genetic diversity. This study aimed to genetically characterize T. gondii isolates from naturally infected chickens (Gallus domesticus) in Santa Catarina state, southern Brazil region. Sera from 133 free-range chickens were analyzed by an immunofluorescence assay (IFA) to detect IgG antibodies against T. gondii. Brain and heart from 30 positive animals, based on IFA (≥ 1:64), were used to isolate the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Gallus domesticus; Genotyping; Brazil. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100139 |
| |
|
|
| Santos,L.M.; Messas,N.B.; Palumbo,M.I.P.; Miyasato,L.A.; Leal,P.V.; Martins,T.B.; Ramos,C.A.N.. |
| ABSTRACT Mutant color alopecia is an ectodermical defection of color dilution, characterized by partial alopecia, dry, shine-less hair, and peeling and papule. Melanization damages also occur on the cortical structure of the affected hair. The animals affected have big melanin grains with irregular shape on the basal keratinocytes, also on the hair matrix cells and rod. Therefore, there is not a specific treatment that makes any difference on the syndrome evolution. Although in some animals, it is possible to use weekly showers with benzyl peroxide to reduce seborrhea formation and secondary infections. There is evidence that the condition in dogs is caused by a single nucleotide polymorphism in the gene encoding the melanophilin protein. In the present... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotyping; Dermatology; Skin diseases; Mendelian inheritance. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601503 |
| |
|
|
| Dulgheroff,A.C.B.; Pereira,W.A.B.; Sarmento,R.R.; Silva,G.A.V.; Naveca,F.G.; Domingues,A.L.S.. |
| RESUMO O objetivo deste estudo foi detectar rotavírus em fezes de bezerros com diarreia em Uberaba, MG, e caracterizar os genes VP7 e VP4 por meio da genotipagem e da análise filogenética. Setenta e quatro amostras foram coletadas entre novembro de 2008 e setembro de 2009. A detecção do vírus foi feita por teste de aglutinação e as amostras positivas foram submetidas à transcrição reversa, seguida de reação em cadeia da polimerase (RT-PCR), tipagem por PCR e sequenciamento. A taxa de detecção de rotavírus foi de 6,8% e todas as amostras apresentaram o genótipo G6P[5]. A análise filogenética mostrou que as amostras do genótipo G6 pertencem à linhagem IV e que, para ambos os genes (VP7 e VP4), as amostras deste estudo compõem um sub-cluster à parte daquele... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine rotavirus; Genotyping; Genetic lineage. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000401090 |
| |
|
|
| Caballero,Susana; Steel,Debbie; Pallin,Logan; Botero-Acosta,Natalia; Felix,Fernando; Olavarría,Carlos; Diaz granados,Maria Claudia; Bessudo,Sandra; Friedlander,Ari; Scott Baker,C.. |
| ABSTRACT We analysed 114 DNA samples collected in different locations within the stock G breeding grounds, including Ecuador and the northern and southern Pacific of Colombia. We genotyped 15 microsatellite loci, sexed all samples, and performed genotype comparisons. Genotype comparisons were done using a DNA register of previously genotyped individuals from the Colombian breeding ground and the Antarctic Peninsula feeding Area. We confirmed connectivity between feeding grounds in Antarctic Peninsula and breeding grounds in Colombia and Ecuador using microsatellite loci. We found recaptures within each sampling location in the same year, but we also found a few recaptures in the same locations between years both in breeding and feeding grounds.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Megaptera novaeangliae; Southeastern Pacific; Genotyping; Microsatellite; Migratory connectivity.. |
| Ano: 2021 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300031 |
| |
|
|
| Lima,Eleonidas Moura; Lopes,Otávio Sérgio; Soares,Leonardo Ferreira; Arruda,Talitta Dantas; Gigek,Carolina Oliveira; Melo,Cynthia Germoglio Farias; Smith,Marilia Arruda Cardoso; Oliveira,João Ricardo Gonçalves; Medeiros,Arnaldo; Delatorre,Plínio; Burbano,Rommel Rodriguez. |
| Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DSASP method; Genotyping; Gastric Cancer. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414 |
| |
|
|
| Tupinambás,Unaí; Ribeiro,Flávia Andrade; Aleixo,Agdemir; Greco,Dirceu. |
| We assessed the performance of HIV-1 genotyping tests in rescue therapy. Patients were divided into two groups: group 1 (genotyped), included those switching to new antiretroviral drugs based on HIV-1 genotyping data, and group 2 (standard of care -SOC), comprised those in rescue therapy who had not used this test. This was an open and non-randomized study, with 74 patients, followed up for a mean period of 12 months, from February 2002 to May 2003. The groups differed in the duration of antiretroviral use, experience with diverse drug classes (non-nucleoside reverse transcriptase inhibitors and protease inhibitors) and viral load <2.6 log10 copies/mL at any time during treatment. In 23 patients (group 1), the switch in antiretroviral (ARV) regimen was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Genotyping; Antiretroviral resistance testing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200003 |
| |
|
|
| Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho. |
| Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002 |
| |
|
| |
|
|
| Hua,Wenhao; Zhang,Guanbin; Guo,Shujun; Weijie,Li; Sun,Lanhua; Xiang,Guangxin. |
| Background: Research has shown that hepatitis B virus (HBV) genotypes are closely linked to the clinical manifestations, treatment, and prognosis of the disease.Objective: To study the association between genotype and drug-resistant HBV mutations in 620 Chinese patients with chronic HBV infection.Methods: HBV DNA levels were determined using real-time quantitative PCR in plasma samples. Microarrays were performed for the simultaneous detection of HBV genotypes (HBV/B, C, and D) and drug-resistance-related hotspot mutations. A portion of the samples analyzed using microarrays was selected randomly and the data were confirmed using direct DNA sequencing.Results: Most samples were genotype C (471/620; 76.0%), followed by genotype B (149/620; 24.0%). Among the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chronic HBV; Microarray; Genotyping; Drug-resistance mutations. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300291 |
| |
|
|
| Abreu,Juliana Costa de; Vaz,Sara Nunes; Martins Netto,Eduardo; Brites,Carlos. |
| ABSTRACT Objective: To evaluate the virological outcomes in children and adolescents infected with HIV-1 in Salvador, Bahia according to genotyping results. Methods: We retrospectively evaluated the rates of virological suppression of children and adolescents submitted to HIV-1 genotyping test from January/2008 to December/2012. The participants were followed in the two referral centers for pediatric AIDS care, in Salvador, Brazil. Resistance mutations, drug sensitivity profiles, and viral subtypes were analyzed using the Stanford HIV-1 Drug Resistance Database. Adherence was estimated by drugs withdrawal at pharmacies of the two sites. Results: 101 subjects were included: 35 (34.6%) were drug-naïve, and the remaining 66 were failing ART. In drug-naïve... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Children; Virological suppression; Genotyping; Adherence. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300219 |
| |
|
|
| Duarte,Tonya Azevedo; Nery,Joilda Silva; Boechat,Neio; Pereira,Susan Martins; Simonsen,Vera; Oliveira,Martha; Gomes,Maria Gabriela Miranda; Penha-Gonçalves,Carlos; Barreto,Mauricio Lima; Barbosa,Theolis. |
| ABSTRACT Introduction: The Mycobacterium tuberculosis East African-Indian (EAI) spoligotyping family (belonging to lineage 1, Indo-Oceanic, defined by the region of deletion RD239) is distributed worldwide, but is more prevalent in Southeast Asia, India, and East Africa. Studies in Latin America have rarely identified EAI. In this study, we describe the occurrence of the EAI family in Brazil. Methods: EAI was identified in a systematic literature review of genetic diversity studies pertaining to M. tuberculosis in Brazil, as well as in a survey conducted in Salvador, Bahia, located in the northeastern region of this country. Results: The EAI6-BGD1 spoligotyping family and the EAI5 Spoligotype International Type (SIT) 1983 clade were the most frequently... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Phylogeography; Genotyping; Epidemiology. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300317 |
| |
|
| |
|
| |
|
|
| Raka,Lul; Kalenc,Smilja; Bonjak,Zrinka; Budimir,Ana; Katić,Stjepan; ijak,Dubravko; Mulliqi-Osmani,Gjyle; Zoutman,Dick; Jaka,Arbëresha. |
| Infections caused by bacteria of genus Acinetobacter pose a significant health care challenge worldwide. Information on molecular epidemiological investigation of outbreaks caused by Acinetobacter species in Kosova is lacking. The present investigation was carried out to enlight molecular epidemiology of Acinetobacterbaumannii in the Central Intensive Care Unit (CICU) of a University hospital in Kosova using pulse field gel electrophoresis (PFGE). During March - July 2006, A. baumannii was isolated from 30 patients, of whom 22 were infected and 8 were colonised. Twenty patients had ventilator-associated pneumonia, one patient had meningitis, and two had coinfection with bloodstream infection and surgical site infection. The most common diagnoses upon... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotyping; Acinetobacter baumannii; Kosova; Intensive care unit. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600004 |
| |
| Registros recuperados: 56 | |
|
|
|
