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Registros recuperados: 26
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Population structure and impact of recurrent selection on popcorn using EST-SSR markers Agronomy
Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga.
The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.05-9; 2.08.04.00-8 Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium. Melhoramento Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218
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Genetic variability and evidence of founder effect in Hemiodus orthonops (Characiformes: Hemiodontidae) from the upper Paraná River basin, Brazil Biological Sciences
Marques, Liégie Alher; Renesto, Erasmo.
 Hemiodus orthonops is a small fish of the Hemiodontidae family, order Characiformes, with a maximum of 25 cm standard length. Until recently, H. orthonops was an endemic species from the Paraná-Paraguay basin and it was absent from the upper Paraná River basin. Since 2008, it has started to be collected in the upper Paraná River, representing up to 10% of catches. Two population samples of H. orthonops from two localities of the upper Parana River basin (Porto Camargo and Porto Figueira) were analyzed using the allozymes electrophoresis technique. Twenty-one enzymatic loci were detected. The population sample from Porto Camargo displayed a genetic variability (He = 0.1061) higher than that from Porto Figueira (He = 0.0580) and homozygote excess in both of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Fish; Genetic distance; Heterozygosity; Protein polymorphism. genética de populações.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31793
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Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179 Biological Sciences
Santos, Michele Rocha dos; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14)....
Palavras-chave: Allozyme; Genetic diversity; Heterozygosity; Polymorphism; Pisces.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14179
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Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas Biological Sciences
Xavier, Keyla Vitoria Marques; Souza, Eden Silva e; Santos, Erick de Aquino; Diniz, Michely Correia.
Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877
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Allozyme analysis of the four species of Hypostomus (Teleostei: Loricariidae) from the Ivaí river, upper Paraná river basin, Brazil - doi: 10.4025/actascibiolsci.v35i4.16355 Biological Sciences
Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199 in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355
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TP53 mutations and loss of heterozygosity of chromosome 17 in colorectal tumors Braz. J. Genet.
Yamamoto,Lidia; Lopes,Ademar A.; Harb-Gama,Angelito; Nagai,Maria A..
The incidence of TP53 point mutations and loss of heterozygosity (LOH) of chromosome 17 in colorectal tumors was determined in a group of Brazilian patients. We screened DNA samples from tumors and distal normal mucosa of 39 patients with colorectal cancer, for TP53 mutations by PCR-SSCP (single-strand conformation polymorphism) analysis. Chromosome 17 LOH was investigated using six PCR-based polymorphic markers and one VNTR probe. TP53 mutations were demonstrated in 15/39 of the cases. Mutations were distributed among all exons examined (five to eight), the majority of them being G/C to A/T transitions. LOH of chromosome 17p and 17q was detected in 70 and 46% of the tumors, respectively. There was a significant association between TP53 mutations and LOH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TP53 mutations; Heterozygosity; Chromosome 17; Colorectal tumors.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400022
Registros recuperados: 26
Primeira ... 12 ... Última
 

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