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Palavra-chave Immobilization (54) Biodegradation (3) Lipase (3) Saccharomyces cerevisiae (3) Alginate (2) Anaerobiosis (2) Cyclodextrin glucanotransferase (2) Ethanol (2) Morphometry (2) Response surface methodology (2) Β-galactosidase (2) (hyper)thermophilic marine micro-organisms (1) 16S rRNA (1) 5.01.00.00-9 Agronomia biomassa microbiana (1) ATPase (1) Acetonitrile (1) Activity (1) Activity and Stability (1) Adsorption (1) Adubos fosfatados (1) Mais... Tipo do documento Info:eu-repo/semantics/article (39) Journal article (9) Text (3) Info:eu-repo/semantics/other (1) Preprint (1) Ano 2019 (1) 2018 (3) 2017 (4) 2016 (9) 2015 (2) 2014 (5) 2013 (3) 2012 (2) 2011 (2) 2010 (1) 2009 (2) 2008 (5) 2007 (1) 2006 (6) 2005 (2) 2004 (4) 2000 (1) 1998 (1) Mais... País Brazil (40) Chile (9) France (3) Belgium (1) Mexico (1) Idioma Inglês (52) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 54 Primeira ... 1 2 3 ... Última Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption BABT Sugahara,Vanessa Hitomi; Varéa,Geni da Silva. Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842 Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems BABT Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed. ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447 Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone. This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003 A comparative study on fungal laccases immobilized on chitosan BABT Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria. The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccases; Immobilization; Chitosan. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001 Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents BABT Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil. ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462 Use of Coffee Capsules as Support Material in Upflow Anaerobic Fixed Bed Reactors BABT Souza,Gabriela Rezende de; Oliveira,Luiz Fernando Coutinho de; Bello,Italoema Pinheiro; Siniscalchi,Luciene Alves Batista; Fia,Ronaldo; Gandia,Rômulo Marçal. Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic filter; Biomass; Immobilization; Support material; Domestic sewage. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100223 Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles BABT Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de. Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003 Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells BABT Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A.. ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical... Tipo: Info:eu-repo/semantics/article Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405 The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose BABT Vignoli,Josiane Alessandra; Celligoi,Maria Antonia Colabone; Silva,Rui Sérgio Ferreira da; Barros,Márcio de. The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol. Tipo: Info:eu-repo/semantics/article Palavras-chave: Permeabilization; Immobilization; Sucrose; Z. mobilis. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500020 Hydrolysis of whey lactose by immobilized β-Galactosidase BABT Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso. Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019 Characteristics of Immobilized Urease on Grafted Alginate Bead Systems BABT Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H.. This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications... Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Immobilization; Kinetic; Reusability; Storage stability. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147 Histomorphometric analysis of the response of rat skeletal muscle to swimming, immobilization and rehabilitation BJMBR Nascimento,C.C.F.; Padula,N.; Milani,J.G.P.O.; Shimano,A.C.; Martinez,E.Z.; Mattiello-Sverzut,A.C.. The objective of the present study was to determine to what extent, if any, swimming training applied before immobilization in a cast interferes with the rehabilitation process in rat muscles. Female Wistar rats, mean weight 260.52 ± 16.26 g, were divided into 4 groups of 6 rats each: control, 6 weeks under baseline conditions; trained, swimming training for 6 weeks; trained-immobilized, swimming training for 6 weeks and then immobilized for 1 week; trained-immobilized-rehabilitated, swimming training for 6 weeks, immobilized for 1 week and then remobilized with swimming for 2 weeks. The animals were then sacrificed and the soleus and tibialis anterior muscles were dissected, frozen in liquid nitrogen and processed histochemically (H&E and mATPase).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swimming training; Female rat skeletal muscles; Immobilization; Morphometry; ATPase. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900013 Rat hindlimb joint immobilization with acrylic resin orthoses BJMBR da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q.. The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016 Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery BJMBR Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S.. Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011 Limb immobilization alters functional electrophysiological parameters of sciatic nerve BJMBR Alves,J.S.M.; Leal-Cardoso,J.H.; Santos-Júnior,F.F.U.; Carlos,P.S.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Ceccatto,V.M.; Barbosa,R.. Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Sciatic nerve; Compound action potential; Conduction velocity; Rheobase; Chronaxy. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715 Analysis of fracture healing in osteopenic bone caused by disuse: experimental study BJMBR Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C.. Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail... Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708 Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats BJMBR Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C.. The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603 Sulfated fucan as support for antibiotic immobilization BJMBR Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C.. Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C<FONT FACE=Symbol>Ý</FONT>-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives... Tipo: Info:eu-repo/semantics/other Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002 Immobilization and therapeutic passive stretching generate thickening and increase the expression of laminin and dystrophin in skeletal muscle BJMBR Cação-Benedini,L.O.; Ribeiro,P.G.; Prado,C.M.; Chesca,D.L.; Mattiello-Sverzut,A.C.. Extracellular matrix and costamere proteins transmit the concentric, isometric, and eccentric forces produced by active muscle contraction. The expression of these proteins after application of passive tension stimuli to muscle remains unknown. This study investigated the expression of laminin and dystrophin in the soleus muscle of rats immobilized with the right ankle in plantar flexion for 10 days and subsequent remobilization, either by isolated free movement in a cage or associated with passive stretching for up to 10 days. The intensity of the macrophage response was also evaluated. One hundred and twenty-eight female Wistar rats were divided into 8 groups: free for 10 days; immobilized for 10 days; immobilized/free for 1, 3, or 10 days; or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Muscle stretching; Soleus muscle; Laminin; Dystrophin; Mechanotransduction. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600483 Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology BJMBR Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F.. We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P... Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005 Registros recuperados: 54 Primeira ... 1 2 3 ... Última

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