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| Registros recuperados: 56 | |
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| Santos, Fabrine A.; Azevedo, Sergio S.; Mota, Rinaldo Aparecido; Kim, Pommy C.P.; Gomes, A. L. V.; Alves, Clebert José. |
| É relatado o primeiro isolamento de Actinobacillus seminis em um caprino no município de Patos, Estado da Paraíba, região semiárida do Brasil. Em um rebanho com 70 caprinos e 65 ovinos criados juntos, um reprodutor da espécie caprina da raça Moxotó, com quatro anos de idade, apresentou um quadro clínico de orquite e epididimite unilateral com consistência firme. O diagnóstico da infecção por A. seminis foi confirmado pela associação dos achados clínicos, isolamento bacteriano e, PCR e sequenciamento do gene 16S do rRNA. O resultado obtido sugere que A. seminis pode ser uma importante causa de infertilidade em caprinos da região. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation. |
| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25222 |
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| QUINTERO-GIL,Carolina; MIRAZO,Santiago; PARRA-SUESCÚN,Jaime; LÓPEZ-HERRERA,Albeiro; MAINARDI,Victoria; ARBIZA,Juan; ORDÚZ,Sergio. |
| ABSTRACT Hepatitis E virus (HEV) is considered one of the leading causes of acute viral hepatitis worldwide, and about 20 million infections and approximately 57 000 deaths occurred every year. However, little is known about the replicative virus cycle due to the absence of a consensus cell culture model. A549 cell line is considered susceptible to HEV genotype 3, however, both viral strain and cell culture conditions could affect the viral isolation in vitro. The objective of this work was to isolate in vitro an HEV-3 strain obtained from human feces. To this, a genotype 3 HEV strain previously identified by genetic characterization was inoculated in A549 monolayers, and incubated for two hours at 37 °C. Five days post-infection, cells were passaged... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A549 cells; Hepevirus; Isolation; RNA virus. |
| Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300503 |
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| Rocha-e-Silva,R.C.; Cardoso,W.M.; Teixeira,R.S.C.; Albuquerque,Á.H.; Horn,R.V.; Lopes,E.S.; Gomes Filho,V.J.R.; Almeida,C.P.; Santos,I.C.L.; Machado,D.N.; Lima,S.V.G.; Carneiro,I.S.. |
| The present study reports the isolation of Salmonellaenterica in organs of free-living domestic pigeons. In the clinic examination, the presence of feces in the peri-cloacal and abdominal regions were observed, as well as symptoms such as cachexy, incoordination and opisthotonos. Before any therapeutic protocol was applied the bird died and a necropsy was then performed for the removal of spleen, liver, kidney and intestine for bacteriological examination and antibiotic sensitivity test. Salmonella enterica subsp.enterica (O:4,5:i-) and Salmonellaenterica subsp. enterica serovar Typhimurium were isolated from the liver and intestine and the sensitivity test demonstrated that these strains are sensitive to several antibiotics. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Necropsy; Bacteriological examination; Sensitivity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501435 |
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| Pereira,C.G.; Saraiva,G.L.; Santos,M.R.; Assao,V.S.; Fietto,J.L.R.; Bressan,G.C.; Almeida,M.R.; Moreira,M.A.S.; Silva Júnior,A.. |
| RESUMO O vírus da bronquite infecciosa (IBV) é um importante patógeno respiratório presente na avicultura comercial e tem provocado grandes perdas econômicas em todo o mundo. A vacinação é realizada pela indústria produtora de aves, mas continuam surgindo novos sorotipos e variações antigênicas, dificultando o controle de IBV. Nós realizamos uma caracterização molecular de uma cepa de IBV obtida diretamente de tecidos e comparamos com a mesma cepa que havia sido passada três vezes em ovo embrionado. Nós mostramos uma variação significante na sequência viral depois de ter sido isolada em ovo embrionado. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Infectious bronchitis virus; Isolation; Characterization; Stability. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401333 |
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| Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M.. |
| The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monkey; Preantral follicle; Isolation; Ovary; Cebus apella. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009 |
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| Genaro,G.; Schmidek,W.R.; Franci,C.R.. |
| Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008 |
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| Ren,H.J.; Zhang,C.L.; Liu,R.D.; Li,N.; Li,X.G.; Xue,H.K.; Guo,Y.; Wang,Z.Q.; Cui,J.; Ming,L.. |
| The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present study, we established a reproducible method for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (≤20 min). With this method, continuously growing mouse IECs, which can be subcultured over a number of passages, were obtained. The obtained cell lines formed a tight cobblestone-like arrangement, displayed long and... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Intestinal epithelial cells; Crypt; Isolation; Primary culture; Collagenase I; Hyaluronidase. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500604 |
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| Aliabadi,Nasrin; Aminzadeh,Saeed; Karkhane,Ali Asghar; Haghbeen,Kamahldin. |
| Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemical characterization; Chitinase; Cohnella sp. A01; Isolation; Thermostable. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400931 |
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| Silva,Maria Luana Cristiny Rodrigues; Lima,Fabiano da Silva; Gomes,Albério Antônio de Barros; Azevedo,Sérgio Santos de; Alves,Clebert José; Bernardi,Fernanda; Ito,Fumio Honma. |
| To determine the presence of rabies virus in the parotid salivary glands, 12 road-killed rabies-positive hoary foxes (Pseudoalopex vetulus) were tested by using the fluorescent antibody test (FAT) and mouse inoculation test (MIT). All 12 parotid salivary glands were positive for both tests, although in some cases several passages were required. The findings of this study support the importance of the hoary fox as rabies reservoir in the semi-arid region of Paraíba State, Northeastern Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parotid gland; Fox; Rabies virus; Isolation; Paraíba State. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300004 |
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| Puentes,R; Eliopulos,N; Pérez,R; Franco,G; Sosa,K; Bianchi,P; Furtado,A; Hübner,S.O.; Esteves,P.A.. |
| Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine Parvovirus 2c; Isolation; Molecular characterization; South America. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300022 |
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| Issa,Marina de Azevedo; Soares Filho,Paulo Martins; Fonseca Júnior,Antônio Augusto; Hodon,Mikael Arrais; Santos,Lílian Cristian dos; Reis,Jenner Karlisson Pimenta dos; Cerqueira Leite,Rômulo. |
| Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement “A” (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared. Regarding the isolation, there were no significant differences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium bovis; Diagnosis; Middlebrook 7H11; Isolation; Sulphuric acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100139 |
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| Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano. |
| To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016 |
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| Santos,Fabrine Alexandre dos; Azevedo,Edísio Oliveira de; Azevedo,Sérgio Santos de; Garino Júnior,Felício; Mota,Rinaldo Aparecido; Kim,Pomy de Cássia Peixoto; Gomes,Ana Lisa Vale; Alves,Clebert José. |
| The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100027 |
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| Registros recuperados: 56 | |
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